ROUGE

Gene/Protein Characteristic Table for mKIAA0235

Link to : HUGE \| SWISS-PROT/TrEMBL
Features : DNA sequence \| Protein sequence

Accession No. :	AK122225
Description :	Pumilio homolog 2.
Clone Name :	mbh00517 [Vector Info]
Source :	Mouse brain

Features of the cloned DNA sequence	Description

Length: 3570 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
YES

Integrity of 3' end

Length of 3'UTR 287 bp

Genome contig ID gi65532617f_7890684
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
TGATGTAACTGTGTCCCCCCCTCCCGCCCCCCACC
Flanking genome sequence
(175636 - 175685)
----+----*----+----*----+----*----+----*----+----*
AAAAAAAAAAAAGGAAAAAAAGTTGTCACATATTTTGTAAATTTATACCT

Genomic structure deduced by Blast search against draft genome sequences
Comparison with the corresponding KIAA cDNA.

KIAA Alignment based on:

KIAA0235 DNA sequence, AA sequence, Physical map

KIAA	Alignment based on:
KIAA0235	DNA sequence, AA sequence, Physical map

Features of the protein sequence	Description

Coding region: 374..3283
Length: 969 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAH41773	99.8	Pum2 protein [M...	Mus musculus
BAE37556	99.3	unnamed protein...	Mus musculus
XP_858023	97.5	similar to pumi...	Canis lupus fam...
XP_001140429	97.2	similar to Pum2...	Pan troglodytes
XP_001095426	97.0	similar to pumi...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other ROUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	ROUGE_ID
AK122205	2.9e-80	71.9	mKIAA0099

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR000104 308 322 PR00308 Antifreeze protein

IPR000104 344 353 PR00308 Antifreeze protein

HMMPfam IPR001313 631 665 PF00806 Pumilio/Puf RNA-binding

IPR001313 667 701 PF00806 Pumilio/Puf RNA-binding

IPR001313 703 737 PF00806 Pumilio/Puf RNA-binding

IPR001313 739 773 PF00806 Pumilio/Puf RNA-binding

IPR001313 775 809 PF00806 Pumilio/Puf RNA-binding

IPR001313 811 845 PF00806 Pumilio/Puf RNA-binding

IPR001313 847 881 PF00806 Pumilio/Puf RNA-binding

IPR001313 890 924 PF00806 Pumilio/Puf RNA-binding

HMMSmart IPR001313 631 666 SM00025 Pumilio/Puf RNA-binding

IPR001313 667 702 SM00025 Pumilio/Puf RNA-binding

IPR001313 703 738 SM00025 Pumilio/Puf RNA-binding

IPR001313 739 774 SM00025 Pumilio/Puf RNA-binding

IPR001313 775 810 SM00025 Pumilio/Puf RNA-binding

IPR001313 811 846 SM00025 Pumilio/Puf RNA-binding

IPR001313 847 882 SM00025 Pumilio/Puf RNA-binding

IPR001313 890 925 SM00025 Pumilio/Puf RNA-binding

ProfileScan NULL 261 470 PS50310 NULL

NULL 342 388 PS50322 NULL

NULL 504 592 PS50324 NULL

IPR001313 631 666 PS50303 Pumilio/Puf RNA-binding

IPR001313 631 947 PS50302 Pumilio/Puf RNA-binding

IPR001313 667 702 PS50303 Pumilio/Puf RNA-binding

IPR001313 703 738 PS50303 Pumilio/Puf RNA-binding

IPR001313 739 774 PS50303 Pumilio/Puf RNA-binding

IPR001313 775 810 PS50303 Pumilio/Puf RNA-binding

IPR001313 811 846 PS50303 Pumilio/Puf RNA-binding

IPR001313 847 882 PS50303 Pumilio/Puf RNA-binding

IPR001313 883 925 PS50303 Pumilio/Puf RNA-binding

Prediction of transmembrane (TM) segments

Method From To amino acid sequence

- - - -

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR000104	308	322	PR00308	Antifreeze protein
IPR000104	344	353	PR00308	Antifreeze protein
HMMPfam	IPR001313	631	665	PF00806	Pumilio/Puf RNA-binding
IPR001313	667	701	PF00806	Pumilio/Puf RNA-binding
IPR001313	703	737	PF00806	Pumilio/Puf RNA-binding
IPR001313	739	773	PF00806	Pumilio/Puf RNA-binding
IPR001313	775	809	PF00806	Pumilio/Puf RNA-binding
IPR001313	811	845	PF00806	Pumilio/Puf RNA-binding
IPR001313	847	881	PF00806	Pumilio/Puf RNA-binding
IPR001313	890	924	PF00806	Pumilio/Puf RNA-binding
HMMSmart	IPR001313	631	666	SM00025	Pumilio/Puf RNA-binding
IPR001313	667	702	SM00025	Pumilio/Puf RNA-binding
IPR001313	703	738	SM00025	Pumilio/Puf RNA-binding
IPR001313	739	774	SM00025	Pumilio/Puf RNA-binding
IPR001313	775	810	SM00025	Pumilio/Puf RNA-binding
IPR001313	811	846	SM00025	Pumilio/Puf RNA-binding
IPR001313	847	882	SM00025	Pumilio/Puf RNA-binding
IPR001313	890	925	SM00025	Pumilio/Puf RNA-binding
ProfileScan	NULL	261	470	PS50310	NULL
NULL	342	388	PS50322	NULL
NULL	504	592	PS50324	NULL
IPR001313	631	666	PS50303	Pumilio/Puf RNA-binding
IPR001313	631	947	PS50302	Pumilio/Puf RNA-binding
IPR001313	667	702	PS50303	Pumilio/Puf RNA-binding
IPR001313	703	738	PS50303	Pumilio/Puf RNA-binding
IPR001313	739	774	PS50303	Pumilio/Puf RNA-binding
IPR001313	775	810	PS50303	Pumilio/Puf RNA-binding
IPR001313	811	846	PS50303	Pumilio/Puf RNA-binding
IPR001313	847	882	PS50303	Pumilio/Puf RNA-binding
IPR001313	883	925	PS50303	Pumilio/Puf RNA-binding

Method	From	To	amino acid sequence
-	-	-	-

How to obtain mKIAA clone(s) How to obtain anti mKIAA antibodies Back to the ROUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	YES

Length of 3'UTR	287 bp
Genome contig ID	gi65532617f_7890684
PolyA signal sequence (None)	+--------+--------+----*----+---- TGATGTAACTGTGTCCCCCCCTCCCGCCCCCCACC
Flanking genome sequence (175636 - 175685)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAGGAAAAAAAGTTGTCACATATTTTGTAAATTTATACCT

Gene/Protein Characteristic Table for mKIAA0235

Features of the cloned DNA sequence

Features of the protein sequence