##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccII AccIII AflII AhdI AscI
AseI AsnI AspEI AviII AvrII
BbeI BbrPI BciVI BfrI BglI
BglII BlnI BsaBI BsaI BseAI
BsmBI BspEI BssHII BstEII BstPI
BstUI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
FseI FspI HpaI KasI KspI
MamI MluI MroI MvnI NarI
NotI NruI PacI PmaCI PmeI
PmlI PshBI PvuI RsrII SacII
SalI SbfI SfiI SfoI SnaBI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 971 GACGTC GACGT^C
AclI 4223 AACGTT AA^CGTT
AcyI 971 GACGTC G[GA]^CG[CT]C
AgeI 2619 ACCGGT A^CCGGT
AocI 3272 CCTGAGG CC^T[ACGT]AGG
ApaI 3635 GGGCCC GGGCC^C
AspI 678 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BsaAI 3378 CACGTA [CT]AC^GT[GA]
BsaHI 971 GACGTC G[GA]^CG[CT]C
BsaWI 2619 ACCGGT [AT]^CCGG[AT]
BsiEI 731 CGGTCG CG[GA][CT]^CG
BsiWI 3120 CGTACG C^GTACG
BssSI 4292 CTCGTG CACGAG(-5/-1)
Bsu36I 3272 CCTGAGG CC^T[ACGT]AGG
DraI 4803 TTTAAA TTT^AAA
Eco81I 3272 CCTGAGG CC^T[ACGT]AGG
EcoRI 1702 GAATTC G^AATTC
EcoT22I 3081 ATGCAT ATGCA^T
Hin1I 971 GACGTC G[GA]^CG[CT]C
MfeI 4585 CAATTG C^AATTG
MunI 4585 CAATTG C^AATTG
NaeI 35 GCCGGC GCC^GGC
NdeI 5201 CATATG CA^TATG
NgoMIV 35 GCCGGC G^CCGGC
NheI 1625 GCTAGC G^CTAGC
NsiI 3081 ATGCAT ATGCA^T
PaeR7I 3599 CTCGAG C^TCGAG
PflFI 678 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2773 CCAACTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2619 ACCGGT A^CCGGT
PpuMI 4334 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 794 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4223 AACGTT AA^CGTT
SapI 4084 GCTCTTC GCTCTTC(1/4)
ScaI 3606 AGTACT AGT^ACT
SgrAI 34 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 787 CCCGGG CCC^GGG
SpeI 4899 ACTAGT A^CTAGT
SphI 2337 GCATGC GCATG^C
StuI 3823 AGGCCT AGG^CCT
Tth111I 678 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2773 CCAACTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2690 TCTAGA T^CTAGA
XhoI 3599 CTCGAG C^TCGAG
XmaCI 787 CCCGGG C^CCGGG
XmaI 787 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 1198 GGTACC G^GTACC
2622 GGTACC
Aor51HI 1397 AGCGCT AGC^GCT
1577 AGCGCT
Asp700I 2603 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2858 GAACGGATTC
Asp718I 1198 GGTACC G^GTACC
2622 GGTACC
AvaI 787 CCCGGG C^[CT]CG[GA]G
3599 CTCGAG
BbsI 675 GAAGAC GAAGAC(2/6)
2744 GTCTTC
BcgI 1606 GCAGGTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4980 GCAGAAGTTTCG
BclI 607 TGATCA T^GATCA
4628 TGATCA
BpuAI 675 GAAGAC GAAGAC(2/6)
2744 GTCTTC
BsmI 1804 GAATGC GAATGC(1/-1)
3083 GCATTC
BsoBI 787 CCCGGG C^[CT]CG[GA]G
3599 CTCGAG
BspHI 487 TCATGA T^CATGA
2833 TCATGA
BsrBI 79 CCGCTC CCGCTC(-3/-3)
4329 GAGCGG
BsrFI 35 GCCGGC [GA]^CCGG[CT]
2619 ACCGGT
Bst1107I 4476 GTATAC GTA^TAC
4598 GTATAC
BstBI 2610 TTCGAA TT^CGAA
3561 TTCGAA
BstXI 1092 CCAAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1477 CCAATATTATGG
BstZ17I 4476 GTATAC GTA^TAC
4598 GTATAC
Cfr10I 35 GCCGGC [GA]^CCGG[CT]
2619 ACCGGT
DraII 3634 GGGGCCC [GA]G^G[ACGT]CC[CT]
4334 GGGACCC
Eco47III 1397 AGCGCT AGC^GCT
1577 AGCGCT
EcoO109I 3634 GGGGCCC [GA]G^G[ACGT]CC[CT]
4334 GGGACCC
FbaI 607 TGATCA T^GATCA
4628 TGATCA
HincII 1294 GTTGAC GT[CT]^[GA]AC
5102 GTCAAC
HindII 1294 GTTGAC GT[CT]^[GA]AC
5102 GTCAAC
HindIII 2413 AAGCTT A^AGCTT
2922 AAGCTT
KpnI 1198 GGTACC GGTAC^C
2622 GGTACC
MslI 3448 CACCATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4896 CACACTAGTG
NcoI 1146 CCATGG C^CATGG
1496 CCATGG
NspV 2610 TTCGAA TT^CGAA
3561 TTCGAA
RcaI 487 TCATGA T^CATGA
2833 TCATGA
SacI 778 GAGCTC GAGCT^C
1830 GAGCTC
SexAI 459 ACCAGGT A^CC[AT]GGT
679 ACCTGGT
SfuI 2610 TTCGAA TT^CGAA
3561 TTCGAA
SspI 595 AATATT AAT^ATT
1479 AATATT
XmnI 2603 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2858 GAACGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AvaII BalI BamHI
BanI BanII BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspDI BspLU11I BspMI BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
ClaI DdeI DpnII DsaI EaeI
EarI EcoNI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII