ATG9B ORF clone pF1KE2522

KOP
KOP Flexi HaloTag ORF List search

Flexi ORF Clone pF1KE2522

How to Order Order Information

Clone info.

Species

human

Clone Name

pF1KE2522 Sequence (with vector) Restriction Map

Product ID

FXC31728

HaloTag Clone PID

ORF Sequence

DNA sequence (2772 bp)
Protein sequence (924 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE2522

Symbol

ATG9B

Alias : APG9L2, NOS3AS, SONE

Description

autophagy related 9B, transcript variant 1

Original Clone ID

cp01650

Note

We obtained this clone using the E.coli strain DH10B at 25 deg C.

Features of the cloned ORF sequence

Description

Length: 2772 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 924 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS83242.1 9.3e-177 100.0 ATG9B CCDS42820.1 6.4e-55 47.5 ATG9A The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR007241 322 683 PF04109 Autophagy-related protein 9
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 213 LEDVFQLGQFIFIVTFTTFLLRC 235 SECONDARY 23 2 278 SPLLVLLLVLAAGFWLVQLLRSV 300 PRIMARY 23 3 441 LLAALNLALSPLVLAWQVLHVFY 463 PRIMARY 23 4 527 LARQLVFFAGALFAALLVLTVYD 549 PRIMARY 23 5 554 AVEHVLTAMTALGVTATVARSFI 576 SECONDARY 23 6 625 VSLLEELLSPLLTPLFLLFWFR 646 PRIMARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001303985 924 612205 100.0 100.0 XP_011514367 924 612205 100.0 100.0 XP_011514368 924 612205 100.0 100.0 NP_001070666 839 612204 47.5 82.4 NP_076990 839 612204 47.5 82.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KE2522 Download

>KIEE2522  2772 bp
ATGGTGAGCCGAATGGGCTGGGGGGGGAGAAGAAGGCGGCTGGGGCGGTGGGGAGATCTG
GGGCCCGGATCGGTGCCCCTCCTCCCCATGCCACTGCCACCTCCTCCTCCTCCTTCATGC
CGGGGACCTGGGGGAGGGAGGATCTCCATCTTCTCTCTGTCCCCTGCCCCTCATACAAGA
AGCTCCCCCTCCTCATTTTCCCCTCCCACCGCAGGGCCCCCTTGCTCAGTGCTACAGGGG
ACAGGGGCTTCTCAGTCTTGCCACAGTGCTCTCCCTATCCCAGCCACCCCCCCAACACAG
GCTCAACCTGCAATGACACCTGCCTCTGCATCTCCCTCCTGGGGATCCCACTCCACCCCA
CCCCTGGCCCCGGCAACCCCCACTCCCTCACAGCAGTGCCCCCAGGACTCTCCTGGGCTG
CGGGTAGGCCCTTTGATCCCTGAACAGGATTATGAGCGGCTGGAGGACTGTGACCCTGAG
GGGTCCCAAGACTCACCCATCCACGGGGAGGAGCAGCAACCCCTGCTTCATGTCCCTGAA
GGGCTCCGAGGCTCCTGGCATCACATCCAGAACCTGGACAGTTTCTTCACCAAGATCTAC
AGCTACCACCAGCGGAATGGCTTTGCCTGCATCTTGCTGGAGGATGTCTTCCAGCTGGGA
CAATTTATTTTCATTGTCACCTTCACAACCTTCCTCCTTCGATGCGTGGATTACAATGTT
CTCTTTGCCAACCAACCAAGTAACCATACCAGACCTGGGCCGTTCCACAGCAAAGTGACC
CTGTCAGATGCCATCCTACCCTCAGCCCAGTGTGCTGAGAGGATCCGCTCCAGCCCGCTG
CTGGTCCTCCTCCTGGTCCTGGCTGCCGGCTTCTGGCTGGTCCAACTGCTTCGCTCAGTC
TGCAACCTCTTCAGCTACTGGGACATCCAGGTGTTTTACAGGGAGGCCCTGCACATCCCC
CCGGAGGAGCTGAGCTCGGTTCCCTGGGCAGAGGTGCAGTCCCGCCTCTTGGCACTGCAG
CGGAGCGGGGGCCTGTGCGTGCAGCCGCGGCCCCTGACGGAGCTGGACATCCACCACCGC
ATCCTGCGCTACACCAACTACCAGGTGGCGCTGGCCAACAAAGGCCTGCTGCCGGCCCGC
TGCCCGCTGCCCTGGGGAGGCAGTGCGGCTTTCCTCAGCCGCGGCCTGGCGCTCAATGTC
GACCTGCTGCTCTTCCGCGGTCCCTTCTCGCTCTTCCGCGGGGGCTGGGAGCTGCCGCAC
GCCTACAAGCGCAGCGACCAGCGGGGCGCCCTAGCAGCGCGCTGGGGGCGCACAGTGCTG
CTGCTGGCCGCCCTGAACCTGGCGCTGAGCCCGCTGGTGCTGGCCTGGCAGGTTCTGCAC
GTCTTCTATAGCCACGTGGAGCTGCTGCGGCGCGAGCCTGGCGCGCTGGGGGCGCGCGGC
TGGTCCCGCCTGGCGCGCTTGCAGCTGCGCCACTTCAACGAGCTGCCGCACGAGCTGCGC
GCGCGCCTGGCCCGCGCCTACCGCCCCGCCGCCGCCTTCCTGCGCACCGCTGCGCCCCCC
GCGCCCCTGCGCACGCTGCTGGCCCGCCAGCTCGTTTTCTTCGCGGGCGCACTCTTCGCC
GCGCTGCTTGTGCTCACCGTCTACGACGAGGACGTGCTAGCCGTGGAGCACGTGCTCACC
GCCATGACCGCGCTCGGGGTCACCGCCACCGTCGCCAGGTCTTTCATTCCGGAAGAGCAG
TGCCAGGGTCGTGCGCCGCAGCTCCTGCTGCAGACAGCCCTGGCCCACATGCACTACCTC
CCGGAGGAGCCCGGCCCCGGCGGCAGGGACCGCGCCTACCGGCAGATGGCGCAGCTGCTG
CAGTACCGAGCGGTCTCCCTCCTGGAGGAGCTCCTGTCCCCGCTCCTCACCCCGCTGTTT
CTGCTTTTCTGGTTCCGCCCTCGTGCCCTGGAGATTATCGACTTTTTTCATCACTTCACT
GTGGATGTGGCTGGGGTTGGGGACATCTGTTCCTTTGCCCTTATGGATGTGAAGCGCCAC
GGACACCCTCAGTGGCTCTCGGCGGGACAGACTGAGGCCTCGCTGTCTCAGCGTGCGGAG
GACGGCAAGACTGAGCTTTCTTTGATGCGGTTCTCCCTGGCGCATCCACTCTGGCGCCCC
CCAGGGCACAGCTCTAAGTTTCTTGGGCACCTCTGGGGCCGAGTACAACAAGATGCAGCT
GCCTGGGGTGCCACCTCGGCTCGCGGCCCCTCCACCCCGGGGGTGCTCAGCAACTGCACC
TCGCCCCTGCCTGAGGCCTTCCTGGCCAACCTCTTCGTGCACCCTCTCCTGCCTCCGAGA
GATCTGAGCCCGACAGCCCCCTGTCCAGCTGCGGCCACAGCCAGCCTCCTTGCCTCCATT
TCCCGAATTGCCCAGGACCCCAGCTCTGTGTCCCCAGGAGGCACTGGGGGCCAGAAGCTG
GCCCAGCTCCCAGAACTTGCTTCTGCCGAGATGAGTCTCCATGTCATCTACCTGCACCAG
CTTCACCAGCAGCAGCAGCAGCAGGAGCCGTGGGGTGAGGCTGCAGCCTCCATCCTGTCC
AGGCCCTGCTCCAGCCCCTCACAGCCACCCTCGCCTGATGAGGAGAAGCCATCCTGGTCA
AGTGACGGCTCCAGTCCTGCCTCTAGCCCCAGACAACAGTGGGGAACCCAGAAGGCCCGG
AATCTGTTCCCCGGAGGGTTTCAGGTGACCACAGACACCCAGAAGGAGCCTGACCGGGCC
TCTTGCACTGAC

ORF Protein

Cloned ORF protein sequence for pF1KE2522 Download

>KIEE2522  924 aa
MVSRMGWGGRRRRLGRWGDLGPGSVPLLPMPLPPPPPPSCRGPGGGRISIFSLSPAPHTR
SSPSSFSPPTAGPPCSVLQGTGASQSCHSALPIPATPPTQAQPAMTPASASPSWGSHSTP
PLAPATPTPSQQCPQDSPGLRVGPLIPEQDYERLEDCDPEGSQDSPIHGEEQQPLLHVPE
GLRGSWHHIQNLDSFFTKIYSYHQRNGFACILLEDVFQLGQFIFIVTFTTFLLRCVDYNV
LFANQPSNHTRPGPFHSKVTLSDAILPSAQCAERIRSSPLLVLLLVLAAGFWLVQLLRSV
CNLFSYWDIQVFYREALHIPPEELSSVPWAEVQSRLLALQRSGGLCVQPRPLTELDIHHR
ILRYTNYQVALANKGLLPARCPLPWGGSAAFLSRGLALNVDLLLFRGPFSLFRGGWELPH
AYKRSDQRGALAARWGRTVLLLAALNLALSPLVLAWQVLHVFYSHVELLRREPGALGARG
WSRLARLQLRHFNELPHELRARLARAYRPAAAFLRTAAPPAPLRTLLARQLVFFAGALFA
ALLVLTVYDEDVLAVEHVLTAMTALGVTATVARSFIPEEQCQGRAPQLLLQTALAHMHYL
PEEPGPGGRDRAYRQMAQLLQYRAVSLLEELLSPLLTPLFLLFWFRPRALEIIDFFHHFT
VDVAGVGDICSFALMDVKRHGHPQWLSAGQTEASLSQRAEDGKTELSLMRFSLAHPLWRP
PGHSSKFLGHLWGRVQQDAAAWGATSARGPSTPGVLSNCTSPLPEAFLANLFVHPLLPPR
DLSPTAPCPAAATASLLASISRIAQDPSSVSPGGTGGQKLAQLPELASAEMSLHVIYLHQ
LHQQQQQQEPWGEAAASILSRPCSSPSQPPSPDEEKPSWSSDGSSPASSPRQQWGTQKAR
NLFPGGFQVTTDTQKEPDRASCTD

ORF+Vector

Nucleotide Sequence (with vector) for pF1KE2522 Download

>pF1KE2522  5885 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGGTGAGCCGA
ATGGGCTGGGGGGGGAGAAGAAGGCGGCTGGGGCGGTGGGGAGATCTGGGGCCCGGATCG
GTGCCCCTCCTCCCCATGCCACTGCCACCTCCTCCTCCTCCTTCATGCCGGGGACCTGGG
GGAGGGAGGATCTCCATCTTCTCTCTGTCCCCTGCCCCTCATACAAGAAGCTCCCCCTCC
TCATTTTCCCCTCCCACCGCAGGGCCCCCTTGCTCAGTGCTACAGGGGACAGGGGCTTCT
CAGTCTTGCCACAGTGCTCTCCCTATCCCAGCCACCCCCCCAACACAGGCTCAACCTGCA
ATGACACCTGCCTCTGCATCTCCCTCCTGGGGATCCCACTCCACCCCACCCCTGGCCCCG
GCAACCCCCACTCCCTCACAGCAGTGCCCCCAGGACTCTCCTGGGCTGCGGGTAGGCCCT
TTGATCCCTGAACAGGATTATGAGCGGCTGGAGGACTGTGACCCTGAGGGGTCCCAAGAC
TCACCCATCCACGGGGAGGAGCAGCAACCCCTGCTTCATGTCCCTGAAGGGCTCCGAGGC
TCCTGGCATCACATCCAGAACCTGGACAGTTTCTTCACCAAGATCTACAGCTACCACCAG
CGGAATGGCTTTGCCTGCATCTTGCTGGAGGATGTCTTCCAGCTGGGACAATTTATTTTC
ATTGTCACCTTCACAACCTTCCTCCTTCGATGCGTGGATTACAATGTTCTCTTTGCCAAC
CAACCAAGTAACCATACCAGACCTGGGCCGTTCCACAGCAAAGTGACCCTGTCAGATGCC
ATCCTACCCTCAGCCCAGTGTGCTGAGAGGATCCGCTCCAGCCCGCTGCTGGTCCTCCTC
CTGGTCCTGGCTGCCGGCTTCTGGCTGGTCCAACTGCTTCGCTCAGTCTGCAACCTCTTC
AGCTACTGGGACATCCAGGTGTTTTACAGGGAGGCCCTGCACATCCCCCCGGAGGAGCTG
AGCTCGGTTCCCTGGGCAGAGGTGCAGTCCCGCCTCTTGGCACTGCAGCGGAGCGGGGGC
CTGTGCGTGCAGCCGCGGCCCCTGACGGAGCTGGACATCCACCACCGCATCCTGCGCTAC
ACCAACTACCAGGTGGCGCTGGCCAACAAAGGCCTGCTGCCGGCCCGCTGCCCGCTGCCC
TGGGGAGGCAGTGCGGCTTTCCTCAGCCGCGGCCTGGCGCTCAATGTCGACCTGCTGCTC
TTCCGCGGTCCCTTCTCGCTCTTCCGCGGGGGCTGGGAGCTGCCGCACGCCTACAAGCGC
AGCGACCAGCGGGGCGCCCTAGCAGCGCGCTGGGGGCGCACAGTGCTGCTGCTGGCCGCC
CTGAACCTGGCGCTGAGCCCGCTGGTGCTGGCCTGGCAGGTTCTGCACGTCTTCTATAGC
CACGTGGAGCTGCTGCGGCGCGAGCCTGGCGCGCTGGGGGCGCGCGGCTGGTCCCGCCTG
GCGCGCTTGCAGCTGCGCCACTTCAACGAGCTGCCGCACGAGCTGCGCGCGCGCCTGGCC
CGCGCCTACCGCCCCGCCGCCGCCTTCCTGCGCACCGCTGCGCCCCCCGCGCCCCTGCGC
ACGCTGCTGGCCCGCCAGCTCGTTTTCTTCGCGGGCGCACTCTTCGCCGCGCTGCTTGTG
CTCACCGTCTACGACGAGGACGTGCTAGCCGTGGAGCACGTGCTCACCGCCATGACCGCG
CTCGGGGTCACCGCCACCGTCGCCAGGTCTTTCATTCCGGAAGAGCAGTGCCAGGGTCGT
GCGCCGCAGCTCCTGCTGCAGACAGCCCTGGCCCACATGCACTACCTCCCGGAGGAGCCC
GGCCCCGGCGGCAGGGACCGCGCCTACCGGCAGATGGCGCAGCTGCTGCAGTACCGAGCG
GTCTCCCTCCTGGAGGAGCTCCTGTCCCCGCTCCTCACCCCGCTGTTTCTGCTTTTCTGG
TTCCGCCCTCGTGCCCTGGAGATTATCGACTTTTTTCATCACTTCACTGTGGATGTGGCT
GGGGTTGGGGACATCTGTTCCTTTGCCCTTATGGATGTGAAGCGCCACGGACACCCTCAG
TGGCTCTCGGCGGGACAGACTGAGGCCTCGCTGTCTCAGCGTGCGGAGGACGGCAAGACT
GAGCTTTCTTTGATGCGGTTCTCCCTGGCGCATCCACTCTGGCGCCCCCCAGGGCACAGC
TCTAAGTTTCTTGGGCACCTCTGGGGCCGAGTACAACAAGATGCAGCTGCCTGGGGTGCC
ACCTCGGCTCGCGGCCCCTCCACCCCGGGGGTGCTCAGCAACTGCACCTCGCCCCTGCCT
GAGGCCTTCCTGGCCAACCTCTTCGTGCACCCTCTCCTGCCTCCGAGAGATCTGAGCCCG
ACAGCCCCCTGTCCAGCTGCGGCCACAGCCAGCCTCCTTGCCTCCATTTCCCGAATTGCC
CAGGACCCCAGCTCTGTGTCCCCAGGAGGCACTGGGGGCCAGAAGCTGGCCCAGCTCCCA
GAACTTGCTTCTGCCGAGATGAGTCTCCATGTCATCTACCTGCACCAGCTTCACCAGCAG
CAGCAGCAGCAGGAGCCGTGGGGTGAGGCTGCAGCCTCCATCCTGTCCAGGCCCTGCTCC
AGCCCCTCACAGCCACCCTCGCCTGATGAGGAGAAGCCATCCTGGTCAAGTGACGGCTCC
AGTCCTGCCTCTAGCCCCAGACAACAGTGGGGAACCCAGAAGGCCCGGAATCTGTTCCCC
GGAGGGTTTCAGGTGACCACAGACACCCAGAAGGAGCCTGACCGGGCCTCTTGCACTGAC
GTTTAAACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCA
AGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGA
GCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAA
AGGAGGAACTATATCCGGTTCGCTTGCTGTCCATAAAACCGCCCAGTCTAGCTATCGCCA
TGTAAGCCCACTGCAAGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGAT
AGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGT
GTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCGTGAGCTTCAAAA
GAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATG
GCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGA
TGACGGTCGTTTCGCATGCTTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGG
GTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCC
GTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGT
GCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTT
CCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGC
GAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATC
ATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCAC
CAAGCGAAACATCGCATCGAGCGAGCACGCACTCGGATGGAAGCCGGTCTTGTCGATCAG
GATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAG
GCGCGTATGCCGGATGGTGAGGATCTCGTCGTGACTCATGGCGATGCCTGCTTGCCGAAT
ATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCG
GACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAA
TGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCC
TTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACC
AAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATC
AGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAA
AAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAA
TCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCC
CCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTC
CGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAG
TTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGA
CCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATC
GCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTAC
AGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTG
CGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACA
AACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAA
AGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAA
CTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTT
ATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATG
AAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCAT
AGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGT
TGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAA
TTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCA
GTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTTTTCAGCCTGATACAGAT
TAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGT
GGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGT
GGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGT
CGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGA
CAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAG
GACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCC
TTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCAT