(Circular) (Six-base) MAPSORT of: pF1KE2522.seq Check: 5618 from: 1 to: 5885 pF1KE2522 5885 bp With 182 enzymes: SgfI * April 28, 2014 10:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 435 435 Size: 5885 AccI GT'mk_AC Cuts at: 1307 1748 2923 1307 Size: 441 1175 4269 Fragments arranged by size: 4269 1175 441 Acc65I G'GTAC_C Cuts at: 2901 2901 Size: 5885 AclI AA'CG_TT Cuts at: 5725 5725 Size: 5885 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 665 1002 3710 4142 4889 5157 665 Size: 337 2708 432 747 268 1393 Fragments arranged by size: 2708 1393 747 432 337 268 AflIII A'CryG_T Cuts at: 3237 4341 5318 3237 Size: 1104 977 3804 Fragments arranged by size: 3804 1104 977 AgeI A'CCGG_T Cuts at: 4273 4273 Size: 5885 AleI CACnn'nnGTG Cuts at: 501 501 Size: 5885 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1441 1441 Size: 5885 AlwNI CAG_nnn'CTG Cuts at: 681 1025 1482 1872 1965 2381 2565 2693 3190 Size: 344 457 390 93 416 184 128 497 Cuts at: 3190 4757 681 Size: 1567 1809 Fragments arranged by size: 1809 1567 497 457 416 390 344 184 128 93 ApaI G_GGCC'C Cuts at: 173 326 173 Size: 153 5732 ApaLI G'TGCA_C Cuts at: 2425 4655 2425 Size: 2230 3655 ApoI r'AATT_y Cuts at: 2889 5238 5494 2889 Size: 2349 256 3280 Fragments arranged by size: 3280 2349 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5885 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5885 AvaI C'yCGr_G Cuts at: 1801 2364 2905 1801 Size: 563 541 4781 Fragments arranged by size: 4781 563 541 BamHI G'GATC_C Cuts at: 451 929 2910 451 Size: 478 1981 3426 Fragments arranged by size: 3426 1981 478 BanI G'GyrC_C Cuts at: 180 1393 2261 2294 2335 2901 3315 3563 3598 Size: 1213 868 33 41 566 414 248 35 Cuts at: 3598 180 Size: 2467 Fragments arranged by size: 2467 1213 868 566 414 248 41 35 33 BanII G_rGCy'C Cuts at: 173 326 653 1084 1459 1919 2000 2458 2899 Size: 153 327 431 375 460 81 458 441 Cuts at: 2899 3929 173 Size: 1030 2129 Fragments arranged by size: 2129 1030 460 458 441 431 375 327 153 81 BbeI G_GCGC'C Cuts at: 1397 2265 3319 3567 1397 Size: 868 1054 248 3715 Fragments arranged by size: 3715 1054 868 248 BbsI GAAGACnn'nnnn_ Cuts at: 747 1482 747 Size: 735 5150 BbvCI CC'TCA_GC Cuts at: 909 1282 909 Size: 373 5512 BciVI GTATCCnnnnn_n' Cuts at: 3783 4544 5885 3783 Size: 761 1341 3783 Fragments arranged by size: 3783 1341 761 BclI T'GATC_A Cuts at: 3405 3405 Size: 5885 BglI GCCn_nnn'nGGC Cuts at: 122 1928 2344 2410 122 Size: 1806 416 66 3597 Fragments arranged by size: 3597 1806 416 66 BglII A'GATC_T Cuts at: 162 701 2448 3400 5437 162 Size: 539 1747 952 2037 610 Fragments arranged by size: 2037 1747 952 610 539 BlpI GC'TnA_GC Cuts at: 1078 1453 2374 2997 1078 Size: 375 921 623 3966 Fragments arranged by size: 3966 921 623 375 Bme1580I G_kGCm'C Cuts at: 173 185 326 508 2055 2276 2297 2429 3510 Size: 12 141 182 1547 221 21 132 1081 Cuts at: 3510 3603 4659 173 Size: 93 1056 1399 Fragments arranged by size: 1547 1399 1081 1056 221 182 141 132 93 21 12 BmgBI CAC'GTC Cuts at: 1488 1761 1488 Size: 273 5612 BmrI ACTGGGnnnn_n' Cuts at: 909 1035 2561 3097 3178 3513 5657 909 Size: 126 1526 536 81 335 2144 1137 Fragments arranged by size: 2144 1526 1137 536 335 126 81 BmtI G_CTAG'C Cuts at: 1768 1768 Size: 5885 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1098 4252 1098 Size: 3154 2731 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 589 766 921 2011 2077 2682 2742 589 Size: 177 155 1090 66 605 60 3732 Fragments arranged by size: 3732 1090 605 177 155 66 60 Bpu10I CC'TnA_GC Cuts at: 909 1282 909 Size: 373 5512 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3061 3940 4432 4730 3061 Size: 879 492 298 4216 Fragments arranged by size: 4216 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 1986 5589 1986 Size: 3603 2282 BsaAI yAC'GTr Cuts at: 1503 1779 3238 1503 Size: 276 1459 4150 Fragments arranged by size: 4150 1459 276 BsaBI GATnn'nnATC Cuts at: 6 253 3399 5436 6 Size: 247 3146 2037 455 Fragments arranged by size: 3146 2037 455 247 BsaHI Gr'CG_yC Cuts at: 1394 2262 3316 3564 4266 5762 1394 Size: 868 1054 248 702 1496 1517 Fragments arranged by size: 1517 1496 1054 868 702 248 BsaWI w'CCGG_w Cuts at: 1836 3074 3595 4273 4547 4694 5105 1836 Size: 1238 521 678 274 147 411 2616 Fragments arranged by size: 2616 1238 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 474 504 1987 2017 474 Size: 30 1483 30 4342 Fragments arranged by size: 4342 1483 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 178 200 203 206 288 631 946 1087 1927 Size: 22 3 3 82 343 315 141 840 Cuts at: 1927 2002 2008 2743 178 Size: 75 6 735 3320 Fragments arranged by size: 3320 840 735 343 315 141 82 75 22 6 3 3 BseYI C'CCAG_C Cuts at: 125 147 387 762 1352 1409 1533 2098 2527 Size: 22 240 375 590 57 124 565 429 Cuts at: 2527 2570 3310 4068 4645 5141 125 Size: 43 740 758 577 496 869 Fragments arranged by size: 869 758 740 590 577 565 496 429 375 240 124 57 43 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1042 1123 1168 1468 2367 2605 1042 Size: 81 45 300 899 238 4322 Fragments arranged by size: 4322 899 300 238 81 45 BsiEI CG_ry'CG Cuts at: 104 3427 3473 4681 104 Size: 3323 46 1208 1308 Fragments arranged by size: 3323 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 378 1084 1742 1778 1784 2000 2375 2429 2899 Size: 706 658 36 6 216 375 54 470 Cuts at: 2899 3677 3867 4659 378 Size: 778 190 792 1604 Fragments arranged by size: 1604 792 778 706 658 470 375 216 190 54 36 6 Bsp1286I G_dGCh'C Cuts at: 173 185 326 378 508 653 1084 1459 1742 Size: 12 141 52 130 145 431 375 283 Cuts at: 1742 1778 1784 1919 2000 2055 2276 2297 2375 Size: 36 6 135 81 55 221 21 78 Cuts at: 2375 2429 2458 2899 3510 3603 3677 3867 3929 Size: 54 29 441 611 93 74 190 62 Cuts at: 3929 4659 173 Size: 730 1399 Fragments arranged by size: 1399 730 611 441 431 375 283 221 190 145 141 135 130 93 81 78 74 62 55 54 52 36 29 21 12 6 BspEI T'CCGG_A Cuts at: 1836 5105 1836 Size: 3269 2616 BspHI T'CATG_A Cuts at: 5061 5061 Size: 5885 BspMI ACCTGCnnnn'nnnn_ Cuts at: 423 435 1319 1467 2627 2935 3149 3451 3832 Size: 12 884 148 1160 308 214 302 381 Cuts at: 3832 423 Size: 2476 Fragments arranged by size: 2476 1160 884 381 308 302 214 148 12 BsrBI CCG'CTC Cuts at: 50 564 935 1133 1978 2010 4177 4231 5715 Size: 514 371 198 845 32 2167 54 1484 Cuts at: 5715 5880 50 Size: 165 55 Fragments arranged by size: 2167 1484 845 514 371 198 165 55 54 32 BsrDI GCAATG_nn' Cuts at: 425 3797 425 Size: 3372 2513 BsrFI r'CCGG_y Cuts at: 973 1239 1946 3883 4064 4273 973 Size: 266 707 1937 181 209 2585 Fragments arranged by size: 2585 1937 707 266 209 181 BssHII G'CGCG_C Cuts at: 1405 1529 1540 1561 1605 1607 1609 1405 Size: 124 11 21 44 2 2 5681 Fragments arranged by size: 5681 124 44 21 11 2 2 BssSI C'ACGA_G Cuts at: 1597 2048 4156 4514 1597 Size: 451 2108 358 2968 Fragments arranged by size: 2968 2108 451 358 BstAPI GCAn_nnn'nTGC Cuts at: 630 1482 1602 1872 1965 3262 630 Size: 852 120 270 93 1297 3253 Fragments arranged by size: 3253 1297 852 270 120 93 BstBI TT'CG_AA Cuts at: 4246 4246 Size: 5885 BstEII G'GTnAC_C Cuts at: 1806 2832 1806 Size: 1026 4859 BstXI CCAn_nnnn'nTGG Cuts at: 724 724 Size: 5885 BstYI r'GATC_y Cuts at: 1 162 248 451 701 929 2448 2910 3377 Size: 161 86 203 250 228 1519 462 467 Cuts at: 3377 3400 3735 3981 4993 5079 5091 5437 1 Size: 23 335 246 1012 86 12 346 449 Fragments arranged by size: 1519 1012 467 462 449 346 335 250 246 228 203 161 86 86 23 12 Bsu36I CC'TnA_GG Cuts at: 584 2399 584 Size: 1815 4070 BtgI C'CryG_G Cuts at: 107 609 1153 1287 1323 1344 1769 2145 2656 Size: 502 544 134 36 21 425 376 511 Cuts at: 2656 107 Size: 3336 Fragments arranged by size: 3336 544 511 502 425 376 134 36 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3835 4016 4179 3835 Size: 181 163 5541 Fragments arranged by size: 5541 181 163 BtsI GCAGTG_nn' Cuts at: 199 508 1120 1275 1852 3128 199 Size: 309 612 155 577 1276 2956 Fragments arranged by size: 2956 1276 612 577 309 155 DraI TTT'AAA Cuts at: 2884 2884 Size: 5885 DrdI GACnn_nn'nnGTC Cuts at: 3591 4449 3591 Size: 858 5027 EaeI y'GGCC_r Cuts at: 1220 1433 2411 2480 3470 3644 4035 4062 5407 Size: 213 978 69 990 174 391 27 1345 Cuts at: 5407 1220 Size: 1698 Fragments arranged by size: 1698 1345 990 978 391 213 174 69 27 EagI C'GGCC_G Cuts at: 3470 3470 Size: 5885 EarI CTCTTCn'nnn_ Cuts at: 1021 1324 1345 1726 1835 2425 3908 4118 1021 Size: 303 21 381 109 590 1483 210 2788 Fragments arranged by size: 2788 1483 590 381 303 210 109 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2032 4093 4403 4549 5695 2032 Size: 2061 310 146 1146 2222 Fragments arranged by size: 2222 2061 1146 310 146 EcoICRI GAG'CTC Cuts at: 1082 1998 2897 1082 Size: 916 899 4070 Fragments arranged by size: 4070 916 899 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 589 665 766 921 1002 2011 2077 2682 2742 Size: 76 101 155 81 1009 66 605 60 Cuts at: 2742 3710 4142 4889 5157 589 Size: 968 432 747 268 1317 Fragments arranged by size: 1317 1009 968 747 605 432 268 155 101 81 76 66 60 EcoNI CCTnn'n_nnAGG Cuts at: 239 1909 1990 2398 239 Size: 1670 81 408 3726 Fragments arranged by size: 3726 1670 408 81 EcoO109I rG'GnC_Cy Cuts at: 169 232 322 323 535 590 1053 1137 2523 Size: 63 90 1 212 55 463 84 1386 Cuts at: 2523 2690 3024 169 Size: 167 334 3030 Fragments arranged by size: 3030 1386 463 334 212 167 90 84 63 55 1 EcoRI G'AATT_C Cuts at: 2889 2889 Size: 5885 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3904 3904 Size: 5885 FspI TGC'GCA Cuts at: 1651 1678 3666 1651 Size: 27 1988 3870 Fragments arranged by size: 3870 1988 27 HaeII r_GCGC'y Cuts at: 1219 1300 1397 1453 2145 2265 3319 3567 4589 Size: 81 97 56 692 120 1054 248 1022 Cuts at: 4589 5568 1219 Size: 979 1536 Fragments arranged by size: 1536 1054 1022 979 692 248 120 97 81 56 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 233 265 1090 1122 1987 2019 2515 2547 2722 Size: 32 825 32 865 32 496 32 175 Cuts at: 2722 2754 2851 2883 3727 3757 3759 3789 233 Size: 32 97 32 844 30 2 30 2329 Fragments arranged by size: 2329 865 844 825 496 175 97 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1308 2924 1308 Size: 1616 4269 KasI G'GCGC_C Cuts at: 1393 2261 3315 3563 1393 Size: 868 1054 248 3715 Fragments arranged by size: 3715 1054 868 248 KpnI G_GTAC'C Cuts at: 2905 2905 Size: 5885 MluI A'CGCG_T Cuts at: 5318 5318 Size: 5885 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1014 4556 4740 1014 Size: 3542 184 2159 Fragments arranged by size: 3542 2159 184 MscI TGG'CCA Cuts at: 1222 2413 3646 1222 Size: 1191 1233 3461 Fragments arranged by size: 3461 1233 1191 MslI CAynn'nnrTG Cuts at: 501 1036 1789 3874 4001 501 Size: 535 753 2085 127 2385 Fragments arranged by size: 2385 2085 753 535 127 MspA1I CmG'CkG Cuts at: 720 762 945 1128 1155 1247 1254 1289 1325 Size: 42 183 183 27 92 7 35 36 Cuts at: 1325 1346 1389 1461 1572 1657 1962 2022 2326 Size: 21 43 72 111 85 305 60 304 Cuts at: 2326 2476 2996 3310 3670 4683 4928 720 Size: 150 520 314 360 1013 245 1677 Fragments arranged by size: 1677 1013 520 360 314 305 304 245 183 183 150 111 92 85 72 60 43 42 36 35 27 21 7 NaeI GCC'GGC Cuts at: 975 1241 4066 975 Size: 266 2825 2794 Fragments arranged by size: 2825 2794 266 NarI GG'CG_CC Cuts at: 1394 2262 3316 3564 1394 Size: 868 1054 248 3715 Fragments arranged by size: 3715 1054 868 248 NcoI C'CATG_G Cuts at: 107 107 Size: 5885 NgoMIV G'CCGG_C Cuts at: 973 1239 4064 973 Size: 266 2825 2794 Fragments arranged by size: 2825 2794 266 NheI G'CTAG_C Cuts at: 1764 1764 Size: 5885 NspI r_CATG'y Cuts at: 1899 2938 3438 4345 1899 Size: 1039 500 907 3439 Fragments arranged by size: 3439 1039 907 500 PasI CC'CwG_GG Cuts at: 1091 1259 2269 1091 Size: 168 1010 4707 Fragments arranged by size: 4707 1010 168 PciI A'CATG_T Cuts at: 4341 4341 Size: 5885 PflMI CCAn_nnn'nTGG Cuts at: 681 2565 681 Size: 1884 4001 PfoI T'CCnGG_A Cuts at: 1907 1988 1907 Size: 81 5804 PmeI GTTT'AAAC Cuts at: 2884 2884 Size: 5885 PmlI CAC'GTG Cuts at: 1503 1779 1503 Size: 276 5609 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5024 5056 5024 Size: 32 5853 PpuMI rG'GwC_Cy Cuts at: 232 590 2523 232 Size: 358 1933 3594 Fragments arranged by size: 3594 1933 358 PspOMI G'GGCC_C Cuts at: 169 322 169 Size: 153 5732 PstI C_TGCA'G Cuts at: 1127 1880 1970 2673 2932 3617 1127 Size: 753 90 703 259 685 3395 Fragments arranged by size: 3395 753 703 685 259 90 PvuI CG_AT'CG Cuts at: 104 104 Size: 5885 PvuII CAG'CTG Cuts at: 762 1572 1962 2326 2476 3310 3670 762 Size: 810 390 364 150 834 360 2977 Fragments arranged by size: 2977 834 810 390 364 360 150 RsrII CG'GwC_CG Cuts at: 4080 4080 Size: 5885 SacI G_AGCT'C Cuts at: 1084 2000 2899 1084 Size: 916 899 4070 Fragments arranged by size: 4070 916 899 SacII CC_GC'GG Cuts at: 1156 1290 1326 1347 1156 Size: 134 36 21 5694 Fragments arranged by size: 5694 134 36 21 SalI G'TCGA_C Cuts at: 1306 2922 1306 Size: 1616 4269 SanDI GG'GwC_CC Cuts at: 590 590 Size: 5885 SapI GCTCTTCn'nnn_ Cuts at: 1324 1345 1835 3908 4118 1324 Size: 21 490 2073 210 3091 Fragments arranged by size: 3091 2073 490 210 21 SbfI CC_TGCA'GG Cuts at: 2932 2932 Size: 5885 ScaI AGT'ACT Cuts at: 5402 5402 Size: 5885 SexAI A'CCwGG_T Cuts at: 1208 1208 Size: 5885 SfcI C'TryA_G Cuts at: 33 340 705 1123 1494 1876 1966 2669 2928 Size: 307 365 418 371 382 90 703 259 Cuts at: 2928 3613 4606 4797 33 Size: 685 993 191 1121 Fragments arranged by size: 1121 993 703 685 418 382 371 365 307 259 191 90 SfiI GGCCn_nnn'nGGCC Cuts at: 2410 2410 Size: 5885 SfoI GGC'GCC Cuts at: 1395 2263 3317 3565 1395 Size: 868 1054 248 3715 Fragments arranged by size: 3715 1054 868 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5885 SmaI CCC'GGG Cuts at: 2366 2907 2366 Size: 541 5344 SmlI C'TyrA_G Cuts at: 3040 3955 4447 4709 3040 Size: 915 492 262 4216 Fragments arranged by size: 4216 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5885 SphI G_CATG'C Cuts at: 2938 3438 2938 Size: 500 5385 StuI AGG'CCT Cuts at: 1232 2185 2404 1232 Size: 953 219 4713 Fragments arranged by size: 4713 953 219 StyI C'CwwG_G Cuts at: 107 3019 3373 107 Size: 2912 354 2619 Fragments arranged by size: 2912 2619 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 618 2860 3468 3601 4060 4269 618 Size: 2242 608 133 459 209 2234 Fragments arranged by size: 2242 2234 608 459 209 133 TatI w'GTAC_w Cuts at: 2310 5400 2310 Size: 3090 2795 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1831 1863 5402 5434 1831 Size: 32 3539 32 2282 Fragments arranged by size: 3539 2282 32 32 Tth111I GACn'n_nGTC Cuts at: 888 3682 888 Size: 2794 3091 XbaI T'CTAG_A Cuts at: 2916 2916 Size: 5885 XcmI CCAnnnn_n'nnnnTGG Cuts at: 468 517 721 859 945 1209 3380 468 Size: 49 204 138 86 264 2171 2973 Fragments arranged by size: 2973 2171 264 204 138 86 49 XmaI C'CCGG_G Cuts at: 2364 2905 2364 Size: 541 5344 XmnI GAAnn'nnTTC Cuts at: 2812 2812 Size: 5885 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AloI AscI AvrII BaeI BcgI BfrBI BsiWI BsmI BsmBI BsrGI BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI NruI NsiI PacI PshAI PsiI PspXI PsrI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI