AP2A1 ORF clone pF1KB8539

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Flexi ORF Clone pF1KB8539

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Clone info.

Species

human

Clone Name

pF1KB8539 Sequence (with vector) Restriction Map

Product ID

FXC03782

HaloTag Clone PID

FHC03782

ORF Sequence

DNA sequence (2865 bp)
Protein sequence (955 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527855]

Gene

KIBB8539

Symbol

AP2A1

Alias : ADTAA, AP2-ALPHA, CLAPA1

Description

adaptor-related protein complex 2, alpha 1 subunit, transcript variant 2

Original Clone ID

cs01870

Features of the cloned ORF sequence

Description

Length: 2865 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 955 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS46149.1 0 100.0 AP2A1 CCDS46148.1 1.1e-175 97.7 AP2A1 CCDS73234.1 1.5e-151 82.3 AP2A2 CCDS44512.1 7.9e-151 82.2 AP2A2 CCDS45522.1 2.1e-17 28.6 AP1G1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR002553 31 589 PF01602 Clathrin/coatomer adaptor IPR008152 724 834 PF02883 Clathrin adaptor IPR003164 842 950 PF02296 Clathrin adaptor HMMSmart IPR008152 723 836 SM00809 Clathrin adaptor
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_570603 955 601026 100.0 100.0 XP_011524859 972 601026 98.9 98.5 NP_055018 977 601026 97.7 100.0 XP_011524858 994 601026 96.7 98.5 NP_001229766 940 607242 82.3 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KB8539 Download

>KIBB8539  2865 bp
ATGCCGGCCGTGTCCAAGGGCGATGGGATGCGGGGGCTCGCGGTGTTCATCTCCGACATC
CGGAACTGTAAGAGCAAAGAGGCGGAAATTAAGAGAATCAACAAGGAACTGGCCAACATC
CGCTCCAAGTTCAAAGGAGACAAAGCCTTGGATGGCTACAGTAAGAAAAAATATGTGTGT
AAACTGCTTTTCATCTTCCTGCTTGGCCATGACATTGACTTTGGGCACATGGAGGCTGTG
AATCTGTTGAGTTCCAATAAATACACAGAGAAGCAAATAGGTTACCTGTTCATTTCTGTG
CTGGTGAACTCGAACTCGGAGCTGATCCGCCTCATCAACAACGCCATCAAGAATGACCTG
GCCAGCCGCAACCCCACCTTCATGTGCCTGGCCCTGCACTGCATCGCCAACGTGGGCAGC
CGGGAGATGGGCGAGGCCTTTGCCGCTGACATCCCCCGCATCCTGGTGGCCGGGGACAGC
ATGGACAGTGTCAAGCAGAGTGCGGCCCTGTGCCTCCTTCGACTGTACAAGGCCTCGCCT
GACCTGGTGCCCATGGGCGAGTGGACGGCGCGTGTGGTACACCTGCTCAATGACCAGCAC
ATGGGTGTGGTCACGGCCGCCGTCAGCCTCATCACCTGTCTCTGCAAGAAGAACCCAGAT
GACTTCAAGACGTGCGTCTCTCTGGCTGTGTCGCGCCTGAGCCGGATCGTCTCCTCTGCC
TCCACCGACCTCCAGGACTACACCTACTACTTCGTCCCAGCACCCTGGCTCTCGGTGAAG
CTCCTGCGGCTGCTGCAGTGCTACCCGCCTCCAGAGGATGCGGCTGTGAAGGGGCGGCTG
GTGGAATGTCTGGAGACTGTGCTCAACAAGGCCCAGGAGCCCCCCAAATCCAAGAAGGTG
CAGCATTCCAACGCCAAGAACGCCATCCTCTTCGAGACCATCAGCCTCATCATCCACTAT
GACAGTGAGCCCAACCTCCTGGTTCGGGCCTGCAACCAGCTGGGCCAGTTCCTGCAGCAC
CGGGAGACCAACCTGCGCTACCTGGCCCTGGAGAGCATGTGCACGCTGGCCAGCTCCGAG
TTCTCCCATGAAGCCGTCAAGACGCACATTGACACCGTCATCAATGCCCTCAAGACGGAG
CGGGACGTCAGCGTGCGGCAGCGGGCGGCTGACCTCCTCTACGCCATGTGTGACCGGAGC
AATGCCAAGCAGATCGTGTCGGAGATGCTGCGGTACCTGGAGACGGCAGACTACGCCATC
CGCGAGGAGATCGTCCTGAAGGTGGCCATCCTGGCCGAGAAGTACGCCGTGGACTACAGC
TGGTACGTGGACACCATCCTCAACCTCATCCGCATTGCGGGCGACTACGTGAGTGAGGAG
GTGTGGTACCGTGTGCTACAGATCGTCACCAACCGTGATGACGTCCAGGGCTATGCCGCC
AAGACCGTCTTTGAGGCGCTCCAGGCCCCTGCCTGTCACGAGAACATGGTGAAGGTTGGC
GGCTACATCCTTGGGGAGTTTGGGAACCTGATTGCTGGGGACCCCCGCTCCAGCCCCCCA
GTGCAGTTCTCCCTGCTCCACTCCAAGTTCCATCTGTGCAGCGTGGCCACGCGGGCGCTG
CTGCTGTCCACCTACATCAAGTTCATCAACCTCTTCCCCGAGACCAAGGCCACCATCCAG
GGCGTCCTGCGGGCCGGCTCCCAGCTGCGCAATGCTGACGTGGAGCTGCAGCAGCGAGCC
GTGGAGTACCTCACCCTCAGCTCAGTGGCCAGCACCGACGTCCTGGCCACGGTGCTGGAG
GAGATGCCGCCCTTCCCCGAGCGCGAGTCGTCCATCCTGGCCAAGCTGAAACGCAAGAAG
GGGCCAGGGGCCGGCAGCGCCCTGGACGATGGCCGGAGGGACCCCAGCAGCAACGACATC
AACGGGGGCATGGAGCCCACCCCCAGCACTGTGTCGACGCCCTCGCCCTCCGCCGACCTC
CTGGGGCTGCGGGCAGCCCCTCCCCCGGCAGCACCCCCGGCTTCTGCAGGAGCAGGGAAC
CTTCTGGTGGACGTCTTCGATGGCCCGGCCGCCCAGCCCAGCCTGGGGCCCACCCCCGAG
GAGGCCTTCCTCAGCCCAGGTCCTGAGGACATCGGCCCTCCCATTCCGGAAGCCGATGAG
TTGCTGAATAAGTTTGTGTGTAAGAACAACGGGGTCCTGTTCGAGAACCAGCTGCTGCAG
ATCGGAGTCAAGTCAGAGTTCCGACAGAACCTGGGCCGCATGTATCTCTTCTATGGCAAC
AAGACCTCGGTGCAGTTCCAGAATTTCTCACCCACTGTGGTTCACCCGGGAGACCTCCAG
ACTCAGCTGGCTGTGCAGACCAAGCGCGTGGCGGCGCAGGTGGACGGCGGCGCGCAGGTG
CAGCAGGTGCTCAATATCGAGTGCCTGCGGGACTTCCTGACGCCCCCGCTGCTGTCCGTG
CGCTTCCGGTACGGTGGCGCCCCCCAGGCCCTCACCCTGAAGCTCCCAGTGACCATCAAC
AAGTTCTTCCAGCCCACCGAGATGGCGGCCCAGGATTTCTTCCAGCGCTGGAAGCAGCTG
AGCCTCCCTCAACAGGAGGCGCAGAAAATCTTCAAAGCCAACCACCCCATGGACGCAGAA
GTTACTAAGGCCAAGCTTCTGGGGTTTGGCTCTGCTCTCCTGGACAATGTGGACCCCAAC
CCTGAGAACTTCGTGGGGGCGGGGATCATCCAGACTAAAGCCCTGCAGGTGGGCTGTCTG
CTTCGGCTGGAGCCCAATGCCCAGGCCCAGATGTACCGGCTGACCCTGCGCACCAGCAAG
GAGCCCGTCTCCCGTCACCTGTGTGAGCTGCTGGCACAGCAGTTC

ORF Protein

Cloned ORF protein sequence for pF1KB8539 Download

>KIBB8539  955 aa
MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVC
KLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDL
ASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASP
DLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSA
STDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKV
QHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSE
FSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAI
REEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAA
KTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRAL
LLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLE
EMPPFPERESSILAKLKRKKGPGAGSALDDGRRDPSSNDINGGMEPTPSTVSTPSPSADL
LGLRAAPPPAAPPASAGAGNLLVDVFDGPAAQPSLGPTPEEAFLSPGPEDIGPPIPEADE
LLNKFVCKNNGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFSPTVVHPGDLQ
TQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGAPQALTLKLPVTIN
KFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPN
PENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

ORF+Vector

Nucleotide Sequence (with vector) for pF1KB8539 Download

>pF1KB8539  5978 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGCCGGCCGTG
TCCAAGGGCGATGGGATGCGGGGGCTCGCGGTGTTCATCTCCGACATCCGGAACTGTAAG
AGCAAAGAGGCGGAAATTAAGAGAATCAACAAGGAACTGGCCAACATCCGCTCCAAGTTC
AAAGGAGACAAAGCCTTGGATGGCTACAGTAAGAAAAAATATGTGTGTAAACTGCTTTTC
ATCTTCCTGCTTGGCCATGACATTGACTTTGGGCACATGGAGGCTGTGAATCTGTTGAGT
TCCAATAAATACACAGAGAAGCAAATAGGTTACCTGTTCATTTCTGTGCTGGTGAACTCG
AACTCGGAGCTGATCCGCCTCATCAACAACGCCATCAAGAATGACCTGGCCAGCCGCAAC
CCCACCTTCATGTGCCTGGCCCTGCACTGCATCGCCAACGTGGGCAGCCGGGAGATGGGC
GAGGCCTTTGCCGCTGACATCCCCCGCATCCTGGTGGCCGGGGACAGCATGGACAGTGTC
AAGCAGAGTGCGGCCCTGTGCCTCCTTCGACTGTACAAGGCCTCGCCTGACCTGGTGCCC
ATGGGCGAGTGGACGGCGCGTGTGGTACACCTGCTCAATGACCAGCACATGGGTGTGGTC
ACGGCCGCCGTCAGCCTCATCACCTGTCTCTGCAAGAAGAACCCAGATGACTTCAAGACG
TGCGTCTCTCTGGCTGTGTCGCGCCTGAGCCGGATCGTCTCCTCTGCCTCCACCGACCTC
CAGGACTACACCTACTACTTCGTCCCAGCACCCTGGCTCTCGGTGAAGCTCCTGCGGCTG
CTGCAGTGCTACCCGCCTCCAGAGGATGCGGCTGTGAAGGGGCGGCTGGTGGAATGTCTG
GAGACTGTGCTCAACAAGGCCCAGGAGCCCCCCAAATCCAAGAAGGTGCAGCATTCCAAC
GCCAAGAACGCCATCCTCTTCGAGACCATCAGCCTCATCATCCACTATGACAGTGAGCCC
AACCTCCTGGTTCGGGCCTGCAACCAGCTGGGCCAGTTCCTGCAGCACCGGGAGACCAAC
CTGCGCTACCTGGCCCTGGAGAGCATGTGCACGCTGGCCAGCTCCGAGTTCTCCCATGAA
GCCGTCAAGACGCACATTGACACCGTCATCAATGCCCTCAAGACGGAGCGGGACGTCAGC
GTGCGGCAGCGGGCGGCTGACCTCCTCTACGCCATGTGTGACCGGAGCAATGCCAAGCAG
ATCGTGTCGGAGATGCTGCGGTACCTGGAGACGGCAGACTACGCCATCCGCGAGGAGATC
GTCCTGAAGGTGGCCATCCTGGCCGAGAAGTACGCCGTGGACTACAGCTGGTACGTGGAC
ACCATCCTCAACCTCATCCGCATTGCGGGCGACTACGTGAGTGAGGAGGTGTGGTACCGT
GTGCTACAGATCGTCACCAACCGTGATGACGTCCAGGGCTATGCCGCCAAGACCGTCTTT
GAGGCGCTCCAGGCCCCTGCCTGTCACGAGAACATGGTGAAGGTTGGCGGCTACATCCTT
GGGGAGTTTGGGAACCTGATTGCTGGGGACCCCCGCTCCAGCCCCCCAGTGCAGTTCTCC
CTGCTCCACTCCAAGTTCCATCTGTGCAGCGTGGCCACGCGGGCGCTGCTGCTGTCCACC
TACATCAAGTTCATCAACCTCTTCCCCGAGACCAAGGCCACCATCCAGGGCGTCCTGCGG
GCCGGCTCCCAGCTGCGCAATGCTGACGTGGAGCTGCAGCAGCGAGCCGTGGAGTACCTC
ACCCTCAGCTCAGTGGCCAGCACCGACGTCCTGGCCACGGTGCTGGAGGAGATGCCGCCC
TTCCCCGAGCGCGAGTCGTCCATCCTGGCCAAGCTGAAACGCAAGAAGGGGCCAGGGGCC
GGCAGCGCCCTGGACGATGGCCGGAGGGACCCCAGCAGCAACGACATCAACGGGGGCATG
GAGCCCACCCCCAGCACTGTGTCGACGCCCTCGCCCTCCGCCGACCTCCTGGGGCTGCGG
GCAGCCCCTCCCCCGGCAGCACCCCCGGCTTCTGCAGGAGCAGGGAACCTTCTGGTGGAC
GTCTTCGATGGCCCGGCCGCCCAGCCCAGCCTGGGGCCCACCCCCGAGGAGGCCTTCCTC
AGCCCAGGTCCTGAGGACATCGGCCCTCCCATTCCGGAAGCCGATGAGTTGCTGAATAAG
TTTGTGTGTAAGAACAACGGGGTCCTGTTCGAGAACCAGCTGCTGCAGATCGGAGTCAAG
TCAGAGTTCCGACAGAACCTGGGCCGCATGTATCTCTTCTATGGCAACAAGACCTCGGTG
CAGTTCCAGAATTTCTCACCCACTGTGGTTCACCCGGGAGACCTCCAGACTCAGCTGGCT
GTGCAGACCAAGCGCGTGGCGGCGCAGGTGGACGGCGGCGCGCAGGTGCAGCAGGTGCTC
AATATCGAGTGCCTGCGGGACTTCCTGACGCCCCCGCTGCTGTCCGTGCGCTTCCGGTAC
GGTGGCGCCCCCCAGGCCCTCACCCTGAAGCTCCCAGTGACCATCAACAAGTTCTTCCAG
CCCACCGAGATGGCGGCCCAGGATTTCTTCCAGCGCTGGAAGCAGCTGAGCCTCCCTCAA
CAGGAGGCGCAGAAAATCTTCAAAGCCAACCACCCCATGGACGCAGAAGTTACTAAGGCC
AAGCTTCTGGGGTTTGGCTCTGCTCTCCTGGACAATGTGGACCCCAACCCTGAGAACTTC
GTGGGGGCGGGGATCATCCAGACTAAAGCCCTGCAGGTGGGCTGTCTGCTTCGGCTGGAG
CCCAATGCCCAGGCCCAGATGTACCGGCTGACCCTGCGCACCAGCAAGGAGCCCGTCTCC
CGTCACCTGTGTGAGCTGCTGGCACAGCAGTTCGTTTAAACGAATTCGAGCTCGGTACCC
GGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCG
AAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGC
CTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGTTCGCTTGC
TGTCCATAAAACCGCCCAGTCTAGCTATCGCCATGTAAGCCCACTGCAAGCTACCTGCTT
TCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGG
TCAGCACCGTTTCTGCGGACTGGCTTTCTACGTGTTCCGCTTCCTTTAGCAGCCCTTGCG
CCCTGAGTGCTTGCGGCAGCGTGAGCTTCAAAAGAATTGCCAGCTGGGGCGCCCTCTGGT
AAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGG
CGCAGGGGATCAAGATCTGATCAAGAGACAGGATGACGGTCGTTTCGCATGCTTGAACAA
GATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGG
GCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGC
CCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCA
GCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTC
ACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCA
TCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCAT
ACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCA
CGCACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGG
CTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGTATGCCGGATGGTGAGGATCTC
GTCGTGACTCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCT
GGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCT
ACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTAC
GGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTC
TGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCA
CTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTG
AGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCA
TAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAA
CCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCC
TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGC
GCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCT
GGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCG
TCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAG
GATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTA
CGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGG
AAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTT
TGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTT
TTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAG
ATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGC
TGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGT
GGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCA
GGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTG
ATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTAC
TGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGT
AGAGGATCGAGATCTTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGAT
AAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTC
AGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAA
CTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCT
GTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACG
TTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATC
AAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCAT