Restriction map for pF1KB8539
##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AclI        AflII       AflIII      AgeI        AhdI        
AscI        AseI        AsnI        AspEI       AvrII       
BamHI       BbrPI       BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BsaBI       BsiWI       
BspDI       BspHI       BspLU11I    Bst1107I    BstBI       
BstYI       BstZ17I     ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HpaI        
KspI        MaeI        MamI        MfeI        MflI        
MluI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SapI        ScaI        SfuI        SgrAI       
SnaBI       SpeI        SphI        SspI        SwaI        
XbaI        XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI         1953  GTCGAC               GT^[AC][GT]AC
AcsI         2301  GAATTT               [GA]^AATT[CT]
Alw44I       1059  GTGCAC               G^TGCAC
AocI         2122  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      2564  AGCGCT               AGC^GCT
ApaI         2086  GGGCCC               GGGCC^C
ApaLI        1059  GTGCAC               G^TGCAC
ApoI         2301  GAATTT               [GA]^AATT[CT]
Asp700I      2604  GAAAATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI         2476  GGCGCC               GGCGC^C
BbsI         2053  GTCTTC               GAAGAC(2/6)
BcgI          432  CGAGGCCTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         2577  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2577  GCTGAGC              GC^T[ACGT]AGC
BpuAI        2053  GTCTTC               GAAGAC(2/6)
BsmI          903  GCATTC               GAATGC(1/-1)
Bsp1407I      524  TGTACA               T^GTACA
BsrGI         524  TGTACA               T^GTACA
BssHII       2390  GCGCGC               G^CGCGC
BssSI        1477  CACGAG               CACGAG(-5/-1)
BstAPI       2400  GCAGCAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        280  GGTTACC              G^GT[ACGT]ACC
BstPI         280  GGTTACC              G^GT[ACGT]ACC
Bsu36I       2122  CCTGAGG              CC^T[ACGT]AGG
CelII        2577  GCTGAGC              GC^T[ACGT]AGC
Eco47III     2564  AGCGCT               AGC^GCT
Eco81I       2122  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       280  GGTTACC              G^GT[ACGT]ACC
HincII       1953  GTCGAC               GT[CT]^[GA]AC
HindII       1953  GTCGAC               GT[CT]^[GA]AC
HindIII      2653  AAGCTT               A^AGCTT
KasI         2476  GGCGCC               G^GCGCC
MseI           89  TTAA                 T^TAA
NarI         2476  GGCGCC               GG^CGCC
PshAI         669  GACGTGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI         1953  GTCGAC               G^TCGAC
SbfI         2742  CCTGCAGG             CCTGCA^GG
SexAI         542  ACCTGGT              A^CC[AT]GGT
SfiI         1284  GGCCATCCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         2476  GGCGCC               GGC^GCC
SmaI         2325  CCCGGG               CCC^GGG
SmlI         1129  CTCAAG               C^T[CT][GA]AG
Sse8387I     2742  CCTGCAGG             CCTGCA^GG
SspBI         524  TGTACA               T^GTACA
Tru9I          89  TTAA                 T^TAA
XcmI         2618  CCAACCACCCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        2325  CCCGGG               C^CCGGG
XmaI         2325  CCCGGG               C^CCGGG
XmnI         2604  GAAAATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

Acc65I       1232  GGTACC               G^GTACC
             1385  GGTACC              
AccIII         59  TCCGGA               T^CCGGA
             2145  TCCGGA              
Asp718I      1232  GGTACC               G^GTACC
             1385  GGTACC              
AspI          484  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1111  GACACCGTC           
AviII        1706  TGCGCA               TGC^GCA
             2807  TGCGCA              
BbvCI        1755  CCTCAGC              CCTCAGC(-5/-2)
             2109  CCTCAGC             
BsaAI        1324  TACGTG               [CT]AC^GT[GA]
             1366  TACGTG              
BseAI          59  TCCGGA               T^CCGGA
             2145  TCCGGA              
BspEI          59  TCCGGA               T^CCGGA
             2145  TCCGGA              
FspI         1706  TGCGCA               TGC^GCA
             2807  TGCGCA              
KpnI         1232  GGTACC               GGTAC^C
             1385  GGTACC              
MroI           59  TCCGGA               T^CCGGA
             2145  TCCGGA              
NcoI          551  CCATGG               C^CATGG
             2627  CCATGG              
NspI         1055  GCATGT               [GA]CATG^[CT]
             2258  GCATGT              
PflFI         484  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1111  GACACCGTC           
PflMI         594  CCAGCACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2622  CCACCCCATGG         
TfiI           95  GAATC                G^A[AT]TC
              240  GAATC               
Tsp509I        87  AATT                 ^AATT
             2302  AATT                
Tth111I       484  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1111  GACACCGTC           
Van91I        594  CCAGCACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2622  CCACCCCATGG         


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AatII       AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbvI        
BcnI        BglI        BmyI        BpmI        BsaHI       
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrDI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EagI        EarI        
EclXI       Eco52I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StuI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     
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