Restriction map for pF1KB8539
 (Linear) (Six-base) MAPSORT of: KIBB8539.seq  Check: 5898  from: 1  to: 2865



KIBB8539.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     590    2367    2385    2394    2736    2865 
   Size:        590    1777      18       9     342     129

  Fragments arranged by size: 

                1777     590     342     129      18       9
 
AatII G_ACGT'C

Cuts at:      0    1148    1424    1781    2054    2865 
   Size:       1148     276     357     273     811

  Fragments arranged by size: 

                1148     811     357     276     273
 
AccI GT'mk_AC

Cuts at:      0    1954    2865 
   Size:       1954     911
 
Acc65I G'GTAC_C

Cuts at:      0    1232    1385    2865 
   Size:       1232     153    1480

  Fragments arranged by size: 

                1480    1232     153
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1297    2518    2865 
   Size:       1297    1221     347

  Fragments arranged by size: 

                1297    1221     347
 
AfeI AGC'GCT

Cuts at:      0    2566    2865 
   Size:       2566     299
 
AleI CACnn'nnGTG

Cuts at:      0     602    2840    2865 
   Size:        602    2238      25

  Fragments arranged by size: 

                2238     602      25
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1011    2122    2214    2250    2865 
   Size:       1011    1111      92      36     615

  Fragments arranged by size: 

                1111    1011     615      92      36
 
ApaI G_GGCC'C

Cuts at:      0    2090    2865 
   Size:       2090     775
 
ApaLI G'TGCA_C

Cuts at:      0    1059    2865 
   Size:       1059    1806
 
ApoI r'AATT_y

Cuts at:      0    2301    2865 
   Size:       2301     564
 
AvaI C'yCGr_G

Cuts at:      0    1657    1816    2095    2325    2865 
   Size:       1657     159     279     230     540

  Fragments arranged by size: 

                1657     540     279     230     159
 
BanI G'GyrC_C

Cuts at:      0     546    1232    1385    2476    2865 
   Size:        546     686     153    1091     389

  Fragments arranged by size: 

                1091     686     546     389     153
 
BanII G_rGCy'C

Cuts at:      0      38     881     971    1937    2090    2774    2825    2865 
   Size:         38     843      90     966     153     684      51      40

  Fragments arranged by size: 

                 966     843     684     153      90      51      40      38
            
 
BbeI G_GCGC'C

Cuts at:      0    2480    2865 
   Size:       2480     385
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2046    2865 
   Size:       2046     819
 
BbvCI CC'TCA_GC

Cuts at:      0    1756    2110    2865 
   Size:       1756     354     755

  Fragments arranged by size: 

                1756     755     354
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     421     455    2865 
   Size:        421      34    2410

  Fragments arranged by size: 

                2410     421      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1291    1612    2485    2865 
   Size:       1291     321     873     380

  Fragments arranged by size: 

                1291     873     380     321
 
BlpI GC'TnA_GC

Cuts at:      0    2578    2865 
   Size:       2578     287
 
Bme1580I G_kGCm'C

Cuts at:      0     227     551    1063    2090    2865 
   Size:        227     324     512    1027     775

  Fragments arranged by size: 

                1027     775     512     324     227
 
BmgBI CAC'GTC

Cuts at:      0     671    1719    2865 
   Size:        671    1048    1146

  Fragments arranged by size: 

                1146    1048     671
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1552    2500    2865 
   Size:       1552     948     365

  Fragments arranged by size: 

                1552     948     365
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2603    2844    2865 
   Size:       2603     241      21

  Fragments arranged by size: 

                2603     241      21
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     715     794     871    1069    1258    1444    1533    1816 
   Size:        715      79      77     198     189     186      89     283

Cuts at:   1816    2320    2788    2865 
   Size:        504     468      77

  Fragments arranged by size: 

                 715     504     468     283     198     189     186      89
                  79      77      77
 
Bpu10I CC'TnA_GC

Cuts at:      0     697    1756    2110    2865 
   Size:        697    1059     354     755

  Fragments arranged by size: 

                1059     755     697     354
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1114    2865 
   Size:       1114    1751
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     928    1018    1654    2324    2865 
   Size:        928      90     636     670     541

  Fragments arranged by size: 

                 928     670     636     541      90
 
BsaAI yAC'GTr

Cuts at:      0    1326    1368    2865 
   Size:       1326      42    1497

  Fragments arranged by size: 

                1497    1326      42
 
BsaHI Gr'CG_yC

Cuts at:      0    1145    1421    1682    1778    1957    2051    2440    2477 
   Size:       1145     276     261      96     179      94     389      37

Cuts at:   2477    2865 
   Size:        388

  Fragments arranged by size: 

                1145     389     388     276     261     179      96      94
                  37
 
BsaWI w'CCGG_w

Cuts at:      0      59    1193    2145    2465    2865 
   Size:         59    1134     952     320     400

  Fragments arranged by size: 

                1134     952     400     320      59
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     106     136    1042    1072    1369    1399    1531    1561 
   Size:        106      30     906      30     297      30     132      30

Cuts at:   1561    2865 
   Size:       1304

  Fragments arranged by size: 

                1304     906     297     132     106      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     702    1165    1279    1390    1813    2113    2865 
   Size:        702     463     114     111     423     300     752

  Fragments arranged by size: 

                 752     702     463     423     300     114     111
 
BseYI C'CCAG_C

Cuts at:      0     756     999    1534    1700    1903    1942    2072    2077 
   Size:        756     243     535     166     203      39     130       5

Cuts at:   2077    2865 
   Size:        788

  Fragments arranged by size: 

                 788     756     535     243     203     166     130      39
                   5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379     919    1582    1617    2311    2374    2419    2865 
   Size:        379     540     663      35     694      63      45     446

  Fragments arranged by size: 

                 694     663     540     446     379      63      45      35
            
 
BsiEI CG_ry'CG

Cuts at:      0       8     617    2069    2865 
   Size:          8     609    1452     796

  Fragments arranged by size: 

                1452     796     609       8
 
BsiHKAI G_wGCw'C

Cuts at:      0     863    1063    2411    2865 
   Size:        863     200    1348     454

  Fragments arranged by size: 

                1348     863     454     200
 
BsmI GAATG_Cn'

Cuts at:      0     903    2865 
   Size:        903    1962
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     681     714    1234    2832    2865 
   Size:        681      33     520    1598      33

  Fragments arranged by size: 

                1598     681     520      33      33
 
Bsp1286I G_dGCh'C

Cuts at:      0      38     227     551     863     881     971    1063    1937 
   Size:         38     189     324     312      18      90      92     874

Cuts at:   1937    2090    2411    2774    2825    2865 
   Size:        153     321     363      51      40

  Fragments arranged by size: 

                 874     363     324     321     312     189     153      92
                  90      51      40      38      18
 
BspEI T'CCGG_A

Cuts at:      0      59    2145    2865 
   Size:         59    2086     720

  Fragments arranged by size: 

                2086     720      59
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     590    1040    2367    2385    2394    2736    2865 
   Size:        590     450    1327      18       9     342     129

  Fragments arranged by size: 

                1327     590     450     342     129      18       9
 
BsrBI CCG'CTC

Cuts at:      0     122    1140    1547    2865 
   Size:        122    1018     407    1318

  Fragments arranged by size: 

                1318    1018     407     122
 
BsrDI GCAATG_nn'

Cuts at:      0    1206    1352    1716    2865 
   Size:       1206     146     364    1149

  Fragments arranged by size: 

                1206    1149     364     146
 
BsrFI r'CCGG_y

Cuts at:      0       3    1693    1870    2795    2865 
   Size:          3    1690     177     925      70

  Fragments arranged by size: 

                1690     925     177      70       3
 
BsrGI T'GTAC_A

Cuts at:      0     524    2865 
   Size:        524    2341
 
BssHII G'CGCG_C

Cuts at:      0    2390    2865 
   Size:       2390     475
 
BssSI C'ACGA_G

Cuts at:      0    1477    2865 
   Size:       1477    1388
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2406    2865 
   Size:       2406     459
 
BstEII G'GTnAC_C

Cuts at:      0     280    2865 
   Size:        280    2585
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     764     978    1794    2367    2541    2658    2865 
   Size:        764     214     816     573     174     117     207

  Fragments arranged by size: 

                 816     764     573     214     207     174     117
 
Bsu36I CC'TnA_GG

Cuts at:      0    2123    2865 
   Size:       2123     742
 
BtgI C'CryG_G

Cuts at:      0     551    1307    1739    1787    2627    2865 
   Size:        551     756     432      48     840     238

  Fragments arranged by size: 

                 840     756     551     432     238      48
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      35     387    2865 
   Size:         35     352    2478

  Fragments arranged by size: 

                2478     352      35
 
BtsI GCAGTG_nn'

Cuts at:      0     396     802    2865 
   Size:        396     406    2063

  Fragments arranged by size: 

                2063     406     396
 
EaeI y'GGCC_r

Cuts at:      0       5     110     204     359     467     614    1067    1283 
   Size:          5     105      94     155     108     147     453     216

Cuts at:   1283    1292    1604    1766    1784    1838    1890    2066    2865 
   Size:          9     312     162      18      54      52     176     799

  Fragments arranged by size: 

                 799     453     312     216     176     162     155     147
                 108     105      94      54      52      18       9       5
            
 
EagI C'GGCC_G

Cuts at:      0       5     614    2066    2865 
   Size:          5     609    1452     799

  Fragments arranged by size: 

                1452     799     609       5
 
EarI CTCTTCn'nnn_

Cuts at:      0     934    1657    2272    2865 
   Size:        934     723     615     593

  Fragments arranged by size: 

                 934     723     615     593
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      97     316    1959    2865 
   Size:         97     219    1643     906

  Fragments arranged by size: 

                1643     906     219      97
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     715     794     871    1069    1258    1297    1444    1533 
   Size:        715      79      77     198     189      39     147      89

Cuts at:   1533    1816    2320    2518    2788    2865 
   Size:        283     504     198     270      77

  Fragments arranged by size: 

                 715     504     283     270     198     198     189     147
                  89      79      77      77      39
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1464    1539    1899    2086    2119    2193    2487    2865 
   Size:       1464      75     360     187      33      74     294     378

  Fragments arranged by size: 

                1464     378     360     294     187      75      74      33
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1464    2865 
   Size:       1464    1401
 
FspI TGC'GCA

Cuts at:      0    1708    2809    2865 
   Size:       1708    1101      56

  Fragments arranged by size: 

                1708    1101      56
 
HaeII r_GCGC'y

Cuts at:      0    1459    1618    1880    2480    2568    2865 
   Size:       1459     159     262     600      88     297

  Fragments arranged by size: 

                1459     600     297     262     159      88
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1531    1563    1810    1842    2865 
   Size:       1531      32     247      32    1023

  Fragments arranged by size: 

                1531    1023     247      32      32
 
HincII GTy'rAC

Cuts at:      0    1955    2865 
   Size:       1955     910
 
HindIII A'AGCT_T

Cuts at:      0    2653    2865 
   Size:       2653     212
 
KasI G'GCGC_C

Cuts at:      0    2476    2865 
   Size:       2476     389
 
KpnI G_GTAC'C

Cuts at:      0    1236    1389    2865 
   Size:       1236     153    1476

  Fragments arranged by size: 

                1476    1236     153
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     932    1199    2265    2865 
   Size:         77     855     267    1066     600

  Fragments arranged by size: 

                1066     855     600     267      77
 
MscI TGG'CCA

Cuts at:      0     112     206     361    1069    1285    1606    1768    1786 
   Size:        112      94     155     708     216     321     162      18

Cuts at:   1786    1840    2865 
   Size:         54    1025

  Fragments arranged by size: 

                1025     708     321     216     162     155     112      94
                  54      18
 
MslI CAynn'nnrTG

Cuts at:      0     295     379     463     602     604    2539    2840    2865 
   Size:        295      84      84     139       2    1935     301      25

  Fragments arranged by size: 

                1935     301     295     139      84      84      25       2
            
 
MspA1I CmG'CkG

Cuts at:      0     445     999    1161    1319    1704    2211    2346    2448 
   Size:        445     554     162     158     385     507     135     102

Cuts at:   2448    2577    2865 
   Size:        129     288

  Fragments arranged by size: 

                 554     507     445     385     288     162     158     135
                 129     102
 
NaeI GCC'GGC

Cuts at:      0       5    1695    1872    2865 
   Size:          5    1690     177     993

  Fragments arranged by size: 

                1690     993     177       5
 
NarI GG'CG_CC

Cuts at:      0    2477    2865 
   Size:       2477     388
 
NcoI C'CATG_G

Cuts at:      0     551    2627    2865 
   Size:        551    2076     238

  Fragments arranged by size: 

                2076     551     238
 
NgoMIV G'CCGG_C

Cuts at:      0       3    1693    1870    2865 
   Size:          3    1690     177     995

  Fragments arranged by size: 

                1690     995     177       3
 
NspI r_CATG'y

Cuts at:      0    1059    2262    2865 
   Size:       1059    1203     603

  Fragments arranged by size: 

                1203    1059     603
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     600    2628    2865 
   Size:        600    2028     237

  Fragments arranged by size: 

                2028     600     237
 
PfoI T'CCnGG_A

Cuts at:      0     731    2678    2865 
   Size:        731    1947     187

  Fragments arranged by size: 

                1947     731     187
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     298     330    2865 
   Size:        298      32    2535

  Fragments arranged by size: 

                2535     298      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1539    1899    2119    2193    2865 
   Size:       1539     360     220      74     672

  Fragments arranged by size: 

                1539     672     360     220      74
 
PshAI GACnn'nnGTC

Cuts at:      0     673    2865 
   Size:        673    2192
 
PspOMI G'GGCC_C

Cuts at:      0    2086    2865 
   Size:       2086     779
 
PstI C_TGCA'G

Cuts at:      0     797    1016    1730    2028    2219    2747    2865 
   Size:        797     219     714     298     191     528     118

  Fragments arranged by size: 

                 797     714     528     298     219     191     118
 
PvuII CAG'CTG

Cuts at:      0     999    1319    1704    2211    2346    2577    2865 
   Size:        999     320     385     507     135     231     288

  Fragments arranged by size: 

                 999     507     385     320     288     231     135
 
SalI G'TCGA_C

Cuts at:      0    1953    2865 
   Size:       1953     912
 
SanDI GG'GwC_CC

Cuts at:      0    1539    1899    2865 
   Size:       1539     360     966

  Fragments arranged by size: 

                1539     966     360
 
SbfI CC_TGCA'GG

Cuts at:      0    2747    2865 
   Size:       2747     118
 
SexAI A'CCwGG_T

Cuts at:      0     542    2865 
   Size:        542    2323
 
SfcI C'TryA_G

Cuts at:      0     156     793    1012    1314    1396    1726    2024    2215 
   Size:        156     637     219     302      82     330     298     191

Cuts at:   2215    2743    2865 
   Size:        528     122

  Fragments arranged by size: 

                 637     528     330     302     298     219     191     156
                 122      82
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1291    2865 
   Size:       1291    1574
 
SfoI GGC'GCC

Cuts at:      0    2478    2865 
   Size:       2478     387
 
SmaI CCC'GGG

Cuts at:      0    2327    2865 
   Size:       2327     538
 
SmlI C'TyrA_G

Cuts at:      0    1129    2865 
   Size:       1129    1736
 
StuI AGG'CCT

Cuts at:      0     436     532    2104    2865 
   Size:        436      96    1572     761

  Fragments arranged by size: 

                1572     761     436      96
 
StyI C'CwwG_G

Cuts at:      0      14     146     551    1509    1664    2627    2865 
   Size:         14     132     405     958     155     963     238

  Fragments arranged by size: 

                 963     958     405     238     155     132      14
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     592    2325    2865 
   Size:        592    1733     540

  Fragments arranged by size: 

                1733     592     540
 
TatI w'GTAC_w

Cuts at:      0     524    2865 
   Size:        524    2341
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     555     587    1042    1074    2865 
   Size:        555      32     455      32    1791

  Fragments arranged by size: 

                1791     555     455      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     487    1114    2865 
   Size:        487     627    1751

  Fragments arranged by size: 

                1751     627     487
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2625    2865 
   Size:       2625     240
 
XmaI C'CCGG_G

Cuts at:      0    2325    2865 
   Size:       2325     540
 
XmnI GAAnn'nnTTC

Cuts at:      0    2608    2865 
   Size:       2608     257
 
ZraI GAC'GTC

Cuts at:      0    1146    1422    1779    2052    2865 
   Size:       1146     276     357     273     813

  Fragments arranged by size: 

                1146     813     357     276     273
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI     AleI    AlwNI
     ApaI    ApaLI     ApoI     AvaI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI   BstEII    BstXI   Bsu36I     BtgI    BtgZI     BtsI     EaeI
     EagI     EarI     EciI  Eco57MI EcoO109I     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI    PflMI     PfoI
     PpiI    PpuMI    PshAI   PspOMI     PstI    PvuII     SalI    SanDI
     SbfI    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AclI    AflII   AflIII     AgeI     AhdI     AlfI     AloI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BciVI     BclI    BfrBI
    BglII     BmtI    BsaBI    BsiWI    BspHI    BstBI    BstYI  BstZ17I
     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI    FspAI     HpaI     MfeI     MluI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SapI
     ScaI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     XbaI     XhoI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com