DHX36 ORF clone FXC09599

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Flexi ORF Clone FXC09599

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Clone info.

Species

human

Clone Name

pF1KE2327 Sequence (with vector) Restriction Map

Product ID

FXC09599

HaloTag Clone PID

FHC09599

ORF Sequence

DNA sequence (2937 bp)
Protein sequence (979 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE2327

Symbol

DHX36

Alias : DDX36, G4R1, MLEL1, RHAU

Description

DEAH (Asp-Glu-Ala-His) box polypeptide 36

Original Clone ID

oc01100

Features of the cloned ORF sequence

Description

Length: 2937 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 979 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3171.1 5.2e-166 96.9 DHX36 CCDS54657.1 2.3e-115 95.5 DHX36 CCDS4113.1 2.2e-24 32.3 YTHDC2 CCDS34158.1 6.6e-24 38.8 DHX29 CCDS41444.1 7.4e-24 37.3 DHX9 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR011545 212 373 PF00270 DNA/RNA helicase IPR001650 515 606 PF00271 Helicase IPR007502 670 739 PF04408 Helicase-associated domain IPR011709 774 886 PF07717 Domain of unknown function DUF1605 HMMSmart IPR014001 205 396 SM00487 DEAD-like helicase IPR001650 502 607 SM00490 Helicase IPR007502 669 765 SM00847 Helicase-associated domain ProfileScan IPR014001 217 387 PS51192 DEAD-like helicase IPR001650 477 647 PS51194 Helicase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 703 GKMILFGALFCCLDPVLTIAASL 725 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_065916 1008 612767 96.9 100.0 NP_001107869 994 612767 95.5 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KE2327 Download

>KIEE2327  2937 bp
ATGAGTTATGACTACCATCAGAACTGGGGCCGTGATGGGGGTCCCCGCAGCTCCGGTGGG
GGCTATGGAGGGGGGCCAGCAGGGGGTCATGGAGGTAACCGAGGCTCCGGAGGAGGCGGC
GGCGGCGGAGGGGGTGGTCGAGGCGGCAGGGGCCGGCATCCCGGGCACCTGAAAGGCCGC
GAAATCGGCATGTGGTACGCGAAAAAACAGGGGCAGAAGAACAAGGAAGCGGAGAGGCAA
GAGAGAGCTGTAGTACACATGGATGAACGACGAGAAGAACAAATTGTACAGTTACTGAAT
TCTGTTCAAGCGAAGAATGATAAAGAGTCAGAAGCACAGATATCCTGGTTTGCTCCTGAG
GATCATGGATACGGTACTGAAGTTTCTACTAAGAACACACCATGCTCAGAGAACAAACTT
GACATCCAGGAAAAGAAGTTGATAAATCAAGAAAAAAAAATGTTTAGAATCAGGAACAGA
TCATATATTGACCGAGATTCTGAGTATCTCTTGCAAGAAAATGAACCAGATGGAACTTTA
GACCAAAAATTATTGGAAGATTTACAAAAGAAAAAAAATGACCTTCGGTATATTGAAATG
CAGCATTTCAGAGAAAAGCTGCCTTCGTATGGAATGCAAAAGGAATTGGTAAATTTAATT
GATAACCATCAGGTAACAGTAATAAGTGGTGAAACTGGTTGTGGCAAAACCACTCAAGTT
ACTCAGTTCATTTTGGATAACTACATTGAAAGAGGAAAAGGATCTGCTTGCAGAATAGTT
TGTACTCAGCCAAGAAGAATTAGTGCCATTTCAGTTGCGGAAAGAGTAGCTGCAGAAAGG
GCAGAATCTTGTGGCAGTGGTAATAGTACTGGATATCAAATTCGTCTCCAGAGTCGGTTG
CCAAGGAAACAGGGTTCTATCTTATACTGTACAACAGGAATCATCCTTCAGTGGCTCCAG
TCAGACCCGTATTTGTCCAGTGTTAGTCATATCGTACTTGATGAAATCCATGAAAGAAAT
CTGCAGTCAGATGTTTTAATGACTGTTGTTAAAGACCTTCTCAATTTTCGATCTGACTTG
AAAGTAATATTGATGAGTGCAACATTGAATGCAGAAAAGTTTTCAGAATATTTTGGTAAC
TGTCCAATGATACATATACCTGGTTTTACCTTTCCGGTTGTGGAATATCTTTTGGAAGAT
GTAATTGAAAAAATAAGGTATGTTCCAGAACAAAAAGAACACAGATGCCAGTTTAAGAGG
GGTTTCATGCAAGGGCATGTAAATAGACAAGAAAAAGAAGAAAAAGAAGCAATATATAAA
GAACGTTGGCCAGATTATGTAAGGGAACTGCGAAGAAGGTATTCTGCAAGTACTGTAGAT
GTTATAGAAATGATGGAGGATGATAAAGTTGATCTGAATTTGATTGTTGCCCTCATCCGA
TACATTGTTTTGGAAGAAGAGGATGGTGCGATACTGGTCTTTCTGCCAGGCTGGGACAAT
ATCAGCACTTTACATGATCTCTTGATGTCACAAGTAATGTTTAAATCAGATAAATTTTTA
ATTATACCTTTACATTCACTGATGCCTACAGTTAACCAGACACAGGTGTTTAAAAGAACC
CCTCCTGGTGTTCGGAAAATAGTAATTGCTACCAACATTGCGGAGACTAGCATTACCATA
GATGATGTCGTTTATGTGATAGATGGAGGAAAAATAAAAGAGACGCATTTTGATACTCAG
AACAATAACAGTACAATGTCCGCTGAGTGGGTTAGTAAAGCTAATGCCAAACAGAGAAAA
GGTCGAGCTGGAAGAGTTCAACCTGGTCATTGCTATCATCTGTATAATGGTCTTAGAGCA
AGTCTTCTAGATGACTATCAACTGCCAGAAATTTTGAGAACTCCTTTGGAAGAACTTTGT
TTACAAATAAAGATTTTAAGGCTAGGTGGAATTGCTTATTTTCTGAGTAGATTAATGGAC
CCACCATCAAATGAGGCAGTGTTACTCTCCATAAGACACCTGATGGAGCTGAACGCTTTG
GATAAACAAGAAGAATTGACACCTCTTGGAGTCCACTTGGCACGATTACCCGTTGAGCCA
CATATTGGAAAAATGATTCTTTTTGGAGCACTGTTCTGCTGCTTAGACCCAGTACTCACT
ATTGCTGCTAGTCTCAGTTTCAAAGATCCATTTGTCATTCCACTGGGCTGGGAAGAGGCT
AGGCGACGTGGTTTCAGATACGAAAAGGACTATTGCTGGGAATATTTTCTGTCTTCAAAC
ACACTGCAGATGCTGCATAACATGAAAGGACAGTTTGCTGAGCATCTTCTTGGAGCTGGA
TTTGTAAGCAGTAGAAATCCTAAAGATCCAGAATCTAATATAAATTCAGATAATGAGAAG
ATAATTAAAGCTGTCATCTGTGCTGGTTTATATCCCAAAGTTGCTAAAATTCGACTAAAT
TTGGGTAAAAAAAGAAAAATGGTAAAAGTTTACACAAAAACCGATGGCCTGGTTGCTGTT
CATCCTAAATCTGTTAATGTGGAGCAAACAGACTTTCACTACAACTGGCTTATCTATCAC
CTAAAGATGAGAACAAGCAGTATATACTTGTATGACTGCACAGAGGTTTCCCCATACTGT
CTCTTGTTTTTTGGAGGTGACATTTCCATCCAGAAGGATAACGATCAGGAAACTATTGCT
GTAGATGAGTGGATTGTATTTCAGTCTCCAGCAAGAATTGCCCATCTTGTTAAGGAATTA
AGAAAGGAACTAGATATTCTTCTGCAAGAGAAGATTGAAAGTCCTCATCCTGTAGACTGG
AATGACACTAAATCCAGAGACTGTGCAGTACTGTCAGCTATTATAGACTTGATCAAAACA
CAGGAAAAGGCAACTCCCAGGAACTTTCCGCCACGATTCCAGGATGGATATTACAGC

ORF Protein

Cloned ORF protein sequence for pF1KE2327 Download

>KIEE2327  979 aa
MSYDYHQNWGRDGGPRSSGGGYGGGPAGGHGGNRGSGGGGGGGGGGRGGRGRHPGHLKGR
EIGMWYAKKQGQKNKEAERQERAVVHMDERREEQIVQLLNSVQAKNDKESEAQISWFAPE
DHGYGTEVSTKNTPCSENKLDIQEKKLINQEKKMFRIRNRSYIDRDSEYLLQENEPDGTL
DQKLLEDLQKKKNDLRYIEMQHFREKLPSYGMQKELVNLIDNHQVTVISGETGCGKTTQV
TQFILDNYIERGKGSACRIVCTQPRRISAISVAERVAAERAESCGSGNSTGYQIRLQSRL
PRKQGSILYCTTGIILQWLQSDPYLSSVSHIVLDEIHERNLQSDVLMTVVKDLLNFRSDL
KVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDVIEKIRYVPEQKEHRCQFKR
GFMQGHVNRQEKEEKEAIYKERWPDYVRELRRRYSASTVDVIEMMEDDKVDLNLIVALIR
YIVLEEEDGAILVFLPGWDNISTLHDLLMSQVMFKSDKFLIIPLHSLMPTVNQTQVFKRT
PPGVRKIVIATNIAETSITIDDVVYVIDGGKIKETHFDTQNNNSTMSAEWVSKANAKQRK
GRAGRVQPGHCYHLYNGLRASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLMD
PPSNEAVLLSIRHLMELNALDKQEELTPLGVHLARLPVEPHIGKMILFGALFCCLDPVLT
IAASLSFKDPFVIPLGWEEARRRGFRYEKDYCWEYFLSSNTLQMLHNMKGQFAEHLLGAG
FVSSRNPKDPESNINSDNEKIIKAVICAGLYPKVAKIRLNLGKKRKMVKVYTKTDGLVAV
HPKSVNVEQTDFHYNWLIYHLKMRTSSIYLYDCTEVSPYCLLFFGGDISIQKDNDQETIA
VDEWIVFQSPARIAHLVKELRKELDILLQEKIESPHPVDWNDTKSRDCAVLSAIIDLIKT
QEKATPRNFPPRFQDGYYS

ORF+Vector

Nucleotide Sequence (with vector) for pF1KE2327 Download

>pF1KE2327  6050 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGAGTTATGAC
TACCATCAGAACTGGGGCCGTGATGGGGGTCCCCGCAGCTCCGGTGGGGGCTATGGAGGG
GGGCCAGCAGGGGGTCATGGAGGTAACCGAGGCTCCGGAGGAGGCGGCGGCGGCGGAGGG
GGTGGTCGAGGCGGCAGGGGCCGGCATCCCGGGCACCTGAAAGGCCGCGAAATCGGCATG
TGGTACGCGAAAAAACAGGGGCAGAAGAACAAGGAAGCGGAGAGGCAAGAGAGAGCTGTA
GTACACATGGATGAACGACGAGAAGAACAAATTGTACAGTTACTGAATTCTGTTCAAGCG
AAGAATGATAAAGAGTCAGAAGCACAGATATCCTGGTTTGCTCCTGAGGATCATGGATAC
GGTACTGAAGTTTCTACTAAGAACACACCATGCTCAGAGAACAAACTTGACATCCAGGAA
AAGAAGTTGATAAATCAAGAAAAAAAAATGTTTAGAATCAGGAACAGATCATATATTGAC
CGAGATTCTGAGTATCTCTTGCAAGAAAATGAACCAGATGGAACTTTAGACCAAAAATTA
TTGGAAGATTTACAAAAGAAAAAAAATGACCTTCGGTATATTGAAATGCAGCATTTCAGA
GAAAAGCTGCCTTCGTATGGAATGCAAAAGGAATTGGTAAATTTAATTGATAACCATCAG
GTAACAGTAATAAGTGGTGAAACTGGTTGTGGCAAAACCACTCAAGTTACTCAGTTCATT
TTGGATAACTACATTGAAAGAGGAAAAGGATCTGCTTGCAGAATAGTTTGTACTCAGCCA
AGAAGAATTAGTGCCATTTCAGTTGCGGAAAGAGTAGCTGCAGAAAGGGCAGAATCTTGT
GGCAGTGGTAATAGTACTGGATATCAAATTCGTCTCCAGAGTCGGTTGCCAAGGAAACAG
GGTTCTATCTTATACTGTACAACAGGAATCATCCTTCAGTGGCTCCAGTCAGACCCGTAT
TTGTCCAGTGTTAGTCATATCGTACTTGATGAAATCCATGAAAGAAATCTGCAGTCAGAT
GTTTTAATGACTGTTGTTAAAGACCTTCTCAATTTTCGATCTGACTTGAAAGTAATATTG
ATGAGTGCAACATTGAATGCAGAAAAGTTTTCAGAATATTTTGGTAACTGTCCAATGATA
CATATACCTGGTTTTACCTTTCCGGTTGTGGAATATCTTTTGGAAGATGTAATTGAAAAA
ATAAGGTATGTTCCAGAACAAAAAGAACACAGATGCCAGTTTAAGAGGGGTTTCATGCAA
GGGCATGTAAATAGACAAGAAAAAGAAGAAAAAGAAGCAATATATAAAGAACGTTGGCCA
GATTATGTAAGGGAACTGCGAAGAAGGTATTCTGCAAGTACTGTAGATGTTATAGAAATG
ATGGAGGATGATAAAGTTGATCTGAATTTGATTGTTGCCCTCATCCGATACATTGTTTTG
GAAGAAGAGGATGGTGCGATACTGGTCTTTCTGCCAGGCTGGGACAATATCAGCACTTTA
CATGATCTCTTGATGTCACAAGTAATGTTTAAATCAGATAAATTTTTAATTATACCTTTA
CATTCACTGATGCCTACAGTTAACCAGACACAGGTGTTTAAAAGAACCCCTCCTGGTGTT
CGGAAAATAGTAATTGCTACCAACATTGCGGAGACTAGCATTACCATAGATGATGTCGTT
TATGTGATAGATGGAGGAAAAATAAAAGAGACGCATTTTGATACTCAGAACAATAACAGT
ACAATGTCCGCTGAGTGGGTTAGTAAAGCTAATGCCAAACAGAGAAAAGGTCGAGCTGGA
AGAGTTCAACCTGGTCATTGCTATCATCTGTATAATGGTCTTAGAGCAAGTCTTCTAGAT
GACTATCAACTGCCAGAAATTTTGAGAACTCCTTTGGAAGAACTTTGTTTACAAATAAAG
ATTTTAAGGCTAGGTGGAATTGCTTATTTTCTGAGTAGATTAATGGACCCACCATCAAAT
GAGGCAGTGTTACTCTCCATAAGACACCTGATGGAGCTGAACGCTTTGGATAAACAAGAA
GAATTGACACCTCTTGGAGTCCACTTGGCACGATTACCCGTTGAGCCACATATTGGAAAA
ATGATTCTTTTTGGAGCACTGTTCTGCTGCTTAGACCCAGTACTCACTATTGCTGCTAGT
CTCAGTTTCAAAGATCCATTTGTCATTCCACTGGGCTGGGAAGAGGCTAGGCGACGTGGT
TTCAGATACGAAAAGGACTATTGCTGGGAATATTTTCTGTCTTCAAACACACTGCAGATG
CTGCATAACATGAAAGGACAGTTTGCTGAGCATCTTCTTGGAGCTGGATTTGTAAGCAGT
AGAAATCCTAAAGATCCAGAATCTAATATAAATTCAGATAATGAGAAGATAATTAAAGCT
GTCATCTGTGCTGGTTTATATCCCAAAGTTGCTAAAATTCGACTAAATTTGGGTAAAAAA
AGAAAAATGGTAAAAGTTTACACAAAAACCGATGGCCTGGTTGCTGTTCATCCTAAATCT
GTTAATGTGGAGCAAACAGACTTTCACTACAACTGGCTTATCTATCACCTAAAGATGAGA
ACAAGCAGTATATACTTGTATGACTGCACAGAGGTTTCCCCATACTGTCTCTTGTTTTTT
GGAGGTGACATTTCCATCCAGAAGGATAACGATCAGGAAACTATTGCTGTAGATGAGTGG
ATTGTATTTCAGTCTCCAGCAAGAATTGCCCATCTTGTTAAGGAATTAAGAAAGGAACTA
GATATTCTTCTGCAAGAGAAGATTGAAAGTCCTCATCCTGTAGACTGGAATGACACTAAA
TCCAGAGACTGTGCAGTACTGTCAGCTATTATAGACTTGATCAAAACACAGGAAAAGGCA
ACTCCCAGGAACTTTCCGCCACGATTCCAGGATGGATATTACAGCGTTTAAACGAATTCG
AGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGC
TAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATA
ACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATC
CGGTTCGCTTGCTGTCCATAAAACCGCCCAGTCTAGCTATCGCCATGTAAGCCCACTGCA
AGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGAC
ATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTGTTCCGCTTCCTTTA
GCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCGTGAGCTTCAAAAGAATTGCCAGCTGGG
GCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCA
AGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGACGGTCGTTTCGC
ATGCTTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTC
GGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCA
GCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTG
CAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTG
CTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAG
GATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATG
CGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGC
ATCGAGCGAGCACGCACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAA
GAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGTATGCCGGAT
GGTGAGGATCTCGTCGTGACTCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAAT
GGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGAC
ATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTC
CTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTT
GACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACC
GGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGG
AAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT
GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCA
GAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCT
CGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTC
GGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGT
TCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATC
CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGC
CACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTG
GTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCC
AGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAG
CGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGA
TCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGAT
TTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATAC
AGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTC
AGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGT
TAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTG
TCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGT
AAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGA
TGCGTCCGGCGTAGAGGATCGAGATCTTTTCAGCCTGATACAGATTAAATCAGAACGCAG
AAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCC
CATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGC
GAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCT
TTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAG
CGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAA
CTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTAC
AAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCAT