Restriction map for pF1KE2327
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AatII       Acc65I      AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BanII       BbeI        
BbrPI       BbvCI       BcgI        BfrI        BglI        
BglII       BlnI        BsaAI       BsaHI       BsaI        
BsiEI       BsiWI       BspDI       BspHI       BspLU11I    
BspMI       BsrBI       BssHII      BssSI       Bst1107I    
BstBI       BstZ17I     CfoI        ClaI        CpoI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoT22I     
FseI        FspI        HaeII       HhaI        Hin1I       
HinP1I      HindIII     KasI        KpnI        KspI        
MfeI        MluI        MunI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    StuI        SwaI        Tth111I     XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccI         2812  GTAGAC               GT^[AC][GT]AC
AccIII        106  TCCGGA               T^CCGGA
AclI         1322  AACGTT               AA^CGTT
AocI          355  CCTGAGG              CC^T[ACGT]AGG
AseI         1971  ATTAAT               AT^TAAT
AsnI         1971  ATTAAT               AT^TAAT
AspHI        2126  GAGCAC               G[AT]GC[AT]^C
AvaI          160  CCCGGG               C^[CT]CG[GA]G
BalI         1327  TGGCCA               TGG^CCA
BanI          164  GGCACC               G^G[CT][GA]CC
BciVI         367  GGATAC               GTATCC(6/5)
BclI         2870  TGATCA               T^GATCA
BlpI         2317  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2317  GCTGAGC              GC^T[ACGT]AGC
BseAI         106  TCCGGA               T^CCGGA
BseRI         110  GAGGAG               GAGGAG(10/8)
BsiHKAI      2126  GAGCAC               G[AT]GC[AT]^C
BsoBI         160  CCCGGG               C^[CT]CG[GA]G
BspEI         106  TCCGGA               T^CCGGA
BsrFI         152  GCCGGC               [GA]^CCGG[CT]
BstEII         94  GGTAACC              G^GT[ACGT]ACC
BstPI          94  GGTAACC              G^GT[ACGT]ACC
BstXI        2200  CCACTGGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        355  CCTGAGG              CC^T[ACGT]AGG
CelII        2317  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        152  GCCGGC               [GA]^CCGG[CT]
DraII          39  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EaeI         1327  TGGCCA               [CT]^GGCC[GA]
Eco81I        355  CCTGAGG              CC^T[ACGT]AGG
EcoO109I       39  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I        94  GGTAACC              G^GT[ACGT]ACC
EcoRI         297  GAATTC               G^AATTC
EcoT14I       901  CCAAGG               C^C[AT][AT]GG
FauI           44  CCCGC                CCCGC(4/6)
FbaI         2870  TGATCA               T^GATCA
HgaI         1722  GACGC                GACGC(5/10)
HincII       1591  GTTAAC               GT[CT]^[GA]AC
HindII       1591  GTTAAC               GT[CT]^[GA]AC
HpaI         1591  GTTAAC               GTT^AAC
MluNI        1327  TGGCCA               TGG^CCA
MroI          106  TCCGGA               T^CCGGA
MscI         1327  TGGCCA               TGG^CCA
MslI          256  CACATGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1760  CCGCTG               C[AC]G^C[GT]G
NaeI          152  GCCGGC               GCC^GGC
NgoMIV        152  GCCGGC               G^CCGGC
PflMI        2098  CCACATATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI          39  GGGTCCC              [GA]G^G[AT]CC[CT]
PshBI        1971  ATTAAT               AT^TAAT
Psp1406I     1322  AACGTT               AA^CGTT
SmaI          160  CCCGGG               CCC^GGG
SmlI          713  CTCAAG               C^T[CT][GA]AG
StyI          901  CCAAGG               C^C[AT][AT]GG
Van91I       2098  CCACATATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1866  TCTAGA               T^CTAGA
XmaCI         160  CCCGGG               C^CCGGG
XmaI          160  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccII         178  CGCG                 CG^CG
              198  CGCG                
Asp700I      1355  GAAGGTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2260  GAATATTTTC          
AvaII          40  GGTCC                G^G[AT]CC
             1977  GGACC               
BbsI         1862  GTCTTC               GAAGAC(2/6)
             2271  GTCTTC              
BcnI          160  CCCGG                CC^[GC]GG
              161  CCGGG               
BmyI          163  GGGCAC               G[AGT]GC[ACT]^C
             2126  GAGCAC              
BpuAI        1862  GTCTTC               GAAGAC(2/6)
             2271  GTCTTC              
BsgI         2616  CTGCAC               GTGCAG(16/14)
             2843  GTGCAG              
BsmBI         883  CGTCTC               CGTCTC(1/5)
             1720  GAGACG              
BsmFI        1493  GGGAC                GGGAC(10/14)
               41  GTCCC               
BsmI          632  GAATGC               GAATGC(1/-1)
             1107  GAATGC              
Bsp1286I      163  GGGCAC               G[AGT]GC[ACT]^C
             2126  GAGCAC              
Bsp1407I      285  TGTACA               T^GTACA
              928  TGTACA              
BsrDI        1656  CATTGC               GCAATG(2/0)
             1828  CATTGC              
BsrGI         285  TGTACA               T^GTACA
              928  TGTACA              
BstAPI       2286  GCAGATGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2731  GCAAGAATTGC         
BstUI         178  CGCG                 CG^CG
              198  CGCG                
DraI         1540  TTTAAA               TTT^AAA
             1609  TTTAAA              
EcoRV         339  GATATC               GAT^ATC
              872  GATATC              
MaeII        1323  ACGT                 A^CGT
             2226  ACGT                
MvnI          178  CGCG                 CG^CG
              198  CGCG                
NciI          160  CCCGG                CC^[GC]GG
              161  CCGGG               
NspI          188  GCATGT               [GA]CATG^[CT]
             1275  GCATGT              
SexAI        1158  ACCTGGT              A^CC[AT]GGT
             1821  ACCTGGT             
SspBI         285  TGTACA               T^GTACA
              928  TGTACA              
Tsp45I       1527  GTCAC                ^GT[GC]AC
             2657  GTGAC               
XmnI         1355  GAAGGTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2260  GAATATTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        BbvI        BfaI        BpmI        
BsaBI       BsaJI       BsaWI       BsiYI       BslI        
BsmAI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MamI        MboI        
MboII       MflI        MnlI        MseI        MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        PstI        RsaI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       SfcI        SspI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     XhoII       
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