Restriction map for pF1KE2327
 (Linear) (Six-base) MAPSORT of: KIEE2327.seq  Check: 9619  from: 1  to: 2937



KIEE2327.orf

 With 182 enzymes: SgfI * 

                        February 11, 2011 17:15  ..

AccI GT'mk_AC

Cuts at:      0    2813    2937 
   Size:       2813     124
 
AclI AA'CG_TT

Cuts at:      0    1323    2937 
   Size:       1323    1614
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     398     931    2937 
   Size:        398     533    2006

  Fragments arranged by size: 

                2006     533     398
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2151    2937 
   Size:       2151     786
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     897     929    2937 
   Size:        897      32    2008

  Fragments arranged by size: 

                2008     897      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     294    2292    2840    2937 
   Size:        294    1998     548      97

  Fragments arranged by size: 

                1998     548     294      97
 
ApoI r'AATT_y

Cuts at:      0     297     651     878    1416    1552    1889    2382    2447 
   Size:        297     354     227     538     136     337     493      65

Cuts at:   2447    2457    2937 
   Size:         10     480

  Fragments arranged by size: 

                 538     493     480     354     337     297     227     136
                  65      10
 
AseI AT'TA_AT

Cuts at:      0    1972    2937 
   Size:       1972     965
 
AvaI C'yCGr_G

Cuts at:      0     160    2937 
   Size:        160    2777
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     365     398    2937 
   Size:        365      33    2539

  Fragments arranged by size: 

                2539     365      33
 
BanI G'GyrC_C

Cuts at:      0     164    2937 
   Size:        164    2773
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1855    2264    2937 
   Size:       1855     409     673

  Fragments arranged by size: 

                1855     673     409
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     361    2937 
   Size:        361    2576
 
BclI T'GATC_A

Cuts at:      0    2870    2937 
   Size:       2870      67
 
BlpI GC'TnA_GC

Cuts at:      0    2318    2937 
   Size:       2318     619
 
Bme1580I G_kGCm'C

Cuts at:      0     167    2937 
   Size:        167    2770
 
BmgBI CAC'GTC

Cuts at:      0    2227    2937 
   Size:       2227     710
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      33    2143    2212    2937 
   Size:         33    2110      69     725

  Fragments arranged by size: 

                2110     725      69      33
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     871     940    2711    2937 
   Size:        871      69    1771     226

  Fragments arranged by size: 

                1771     871     226      69
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     698    2937 
   Size:        698    2239
 
BsaBI GATnn'nnATC

Cuts at:      0     665    1874    2681    2937 
   Size:        665    1209     807     256

  Fragments arranged by size: 

                1209     807     665     256
 
BsaWI w'CCGG_w

Cuts at:      0      52     106    1173    2937 
   Size:         52      54    1067    1764

  Fragments arranged by size: 

                1764    1067      54      52
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      88     118    2937 
   Size:         88      30    2819

  Fragments arranged by size: 

                2819      88      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     125    2937 
   Size:        125    2812
 
BseYI C'CCAG_C

Cuts at:      0    1490    2207    2255    2937 
   Size:       1490     717      48     682

  Fragments arranged by size: 

                1490     717     682      48
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2601    2864    2937 
   Size:       2601     263      73

  Fragments arranged by size: 

                2601     263      73
 
BsiHKAI G_wGCw'C

Cuts at:      0    2130    2937 
   Size:       2130     807
 
BsmI GAATG_Cn'

Cuts at:      0     638    1113    2937 
   Size:        638     475    1824

  Fragments arranged by size: 

                1824     638     475
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     889    1714    2937 
   Size:        889     825    1223

  Fragments arranged by size: 

                1223     889     825
 
Bsp1286I G_dGCh'C

Cuts at:      0     167    2130    2937 
   Size:        167    1963     807

  Fragments arranged by size: 

                1963     807     167
 
BspEI T'CCGG_A

Cuts at:      0     106    2937 
   Size:        106    2831
 
BsrDI GCAATG_nn'

Cuts at:      0    1655    1827    2937 
   Size:       1655     172    1110

  Fragments arranged by size: 

                1655    1110     172
 
BsrFI r'CCGG_y

Cuts at:      0     152    2937 
   Size:        152    2785
 
BsrGI T'GTAC_A

Cuts at:      0     285     928    2937 
   Size:        285     643    2009

  Fragments arranged by size: 

                2009     643     285
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2292    2737    2937 
   Size:       2292     445     200

  Fragments arranged by size: 

                2292     445     200
 
BstEII G'GTnAC_C

Cuts at:      0      94    2937 
   Size:         94    2843
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2207    2937 
   Size:       2207     730
 
BstYI r'GATC_y

Cuts at:      0     760    2184    2364    2937 
   Size:        760    1424     180     573

  Fragments arranged by size: 

                1424     760     573     180
 
Bsu36I CC'TnA_GG

Cuts at:      0     356    2937 
   Size:        356    2581
 
BtsI GCAGTG_nn'

Cuts at:      0     861    2003    2281    2937 
   Size:        861    1142     278     656

  Fragments arranged by size: 

                1142     861     656     278
 
DraI TTT'AAA

Cuts at:      0    1542    1611    2937 
   Size:       1542      69    1326

  Fragments arranged by size: 

                1542    1326      69
 
EaeI y'GGCC_r

Cuts at:      0    1327    2937 
   Size:       1327    1610
 
EarI CTCTTCn'nnn_

Cuts at:      0    1451    1806    2207    2937 
   Size:       1451     355     401     730

  Fragments arranged by size: 

                1451     730     401     355
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     140    2897    2937 
   Size:        140    2757      40

  Fragments arranged by size: 

                2757     140      40
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     398     871     931     940    2711    2937 
   Size:        398     473      60       9    1771     226

  Fragments arranged by size: 

                1771     473     398     226      60       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0      40    2937 
   Size:         40    2897
 
EcoRI G'AATT_C

Cuts at:      0     297    2937 
   Size:        297    2640
 
EcoRV GAT'ATC

Cuts at:      0     341     874    2937 
   Size:        341     533    2063

  Fragments arranged by size: 

                2063     533     341
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     310     342    1785    1817    1969    2001    2356    2388 
   Size:        310      32    1443      32     152      32     355      32

Cuts at:   2388    2784    2815    2816    2847    2937 
   Size:        396      31       1      31      90

  Fragments arranged by size: 

                1443     396     355     310     152      90      32      32
                  32      32      31      31       1
 
HincII GTy'rAC

Cuts at:      0    1593    2937 
   Size:       1593    1344
 
HpaI GTT'AAC

Cuts at:      0    1593    2937 
   Size:       1593    1344
 
MscI TGG'CCA

Cuts at:      0    1329    2937 
   Size:       1329    1608
 
MslI CAynn'nnrTG

Cuts at:      0     260    2937 
   Size:        260    2677
 
MspA1I CmG'CkG

Cuts at:      0    1762    2937 
   Size:       1762    1175
 
NaeI GCC'GGC

Cuts at:      0     154    2937 
   Size:        154    2783
 
NgoMIV G'CCGG_C

Cuts at:      0     152    2937 
   Size:        152    2785
 
NspI r_CATG'y

Cuts at:      0     192    1279    2937 
   Size:        192    1087    1658

  Fragments arranged by size: 

                1658    1087     192
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2104    2937 
   Size:       2104     833
 
PfoI T'CCnGG_A

Cuts at:      0     425    2918    2937 
   Size:        425    2493      19

  Fragments arranged by size: 

                2493     425      19
 
PpuMI rG'GwC_Cy

Cuts at:      0      40    2937 
   Size:         40    2897
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     269     301    1904    1936    2937 
   Size:        269      32    1603      32    1001

  Fragments arranged by size: 

                1603    1001     269      32      32
 
PstI C_TGCA'G

Cuts at:      0     834    1025    2288    2937 
   Size:        834     191    1263     649

  Fragments arranged by size: 

                1263     834     649     191
 
SanDI GG'GwC_CC

Cuts at:      0      40    2937 
   Size:         40    2897
 
ScaI AGT'ACT

Cuts at:      0     867    1371    2153    2849    2937 
   Size:        867     504     782     696      88

  Fragments arranged by size: 

                 867     782     696     504      88
 
SexAI A'CCwGG_T

Cuts at:      0    1158    1821    2937 
   Size:       1158     663    1116

  Fragments arranged by size: 

                1158    1116     663
 
SfcI C'TryA_G

Cuts at:      0     248     830    1021    1373    1586    2284    2699    2810 
   Size:        248     582     191     352     213     698     415     111

Cuts at:   2810    2937 
   Size:        127

  Fragments arranged by size: 

                 698     582     415     352     248     213     191     127
                 111
 
SmaI CCC'GGG

Cuts at:      0     162    2937 
   Size:        162    2775
 
SmlI C'TyrA_G

Cuts at:      0     713    2937 
   Size:        713    2224
 
SspI AAT'ATT

Cuts at:      0    1088    1129    2263    2937 
   Size:       1088      41    1134     674

  Fragments arranged by size: 

                1134    1088     674      41
 
StyI C'CwwG_G

Cuts at:      0     901    2937 
   Size:        901    2036
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     506    2937 
   Size:        506    2431
 
TatI w'GTAC_w

Cuts at:      0     252     285     781     865     928    1369    1750    2151 
   Size:        252      33     496      84      63     441     381     401

Cuts at:   2151    2847    2937 
   Size:        696      90

  Fragments arranged by size: 

                 696     496     441     401     381     252      90      84
                  63      33
 
XbaI T'CTAG_A

Cuts at:      0    1866    2937 
   Size:       1866    1071
 
XmaI C'CCGG_G

Cuts at:      0     160    2937 
   Size:        160    2777
 
XmnI GAAnn'nnTTC

Cuts at:      0    1359    2264    2937 
   Size:       1359     905     673

  Fragments arranged by size: 

                1359     905     673
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AlfI     AloI    AlwNI     ApoI     AseI
     AvaI     BaeI     BanI     BbsI    BciVI     BclI     BlpI Bme1580I
    BmgBI     BmrI     BpmI    BpuEI    BsaBI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BsrDI
    BsrFI    BsrGI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtsI
     DraI     EaeI     EarI     EciI  Eco57MI EcoO109I    EcoRI    EcoRV
    Hin4I   HincII     HpaI     MscI     MslI   MspA1I     NaeI   NgoMIV
     NspI    PflMI     PfoI    PpuMI     PsrI     PstI    SanDI     ScaI
    SexAI     SfcI     SmaI     SmlI     SspI     StyI    TaqII     TatI
     XbaI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     ApaI    ApaLI     AscI    AsiSI    AvrII    BamHI    BanII
     BbeI    BbvCI     BcgI    BfrBI     BglI    BglII     BmtI     BplI
   Bpu10I     BsaI    BsaAI    BsaHI    BsiEI    BsiWI    BspHI    BspMI
    BsrBI   BssHII    BssSI    BstBI  BstZ17I     BtgI    BtgZI     ClaI
    CspCI   DraIII     DrdI     EagI  EcoICRI    EcoNI     FalI     FseI
     FspI    FspAI    HaeII  HindIII     KasI     KpnI     MfeI     MluI
     MmeI     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PvuI    PvuII    RsrII     SacI    SacII     SalI
     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI     TstI  Tth111I     XcmI     XhoI
     ZraI
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