GPR148 ORF clone FHC21173

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC21173

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Clone info.

Species

human

Clone Name

pFN21AE9404 Sequence (with vector) Restriction Map

Product ID

FHC21173

ORF Sequence

DNA sequence (1041 bp)
Protein sequence (347 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9404

Symbol

GPR148

Alias : BTR, PGR6

Description

G protein-coupled receptor 148

Original Clone ID

cp99404

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1041 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 347 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2163.1 9.5e-121 99.7 GPR148 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000276 69 250 PF00001 GPCR ProfileScan IPR017452 66 320 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 3 DELAPCPVGTTAWPALIQLISKT 25 SECONDARY 23 2 51 WLFLPSSLLAAATLAVSPLLLVT 73 PRIMARY 23 3 88 LPANILLSDLAYILLHMLISSSS 110 SECONDARY 23 4 124 LTDAVFAACTSTILSFTAIVLHT 146 PRIMARY 23 5 165 AWKAVALIWLVACCFPTFLIWL 186 PRIMARY 22 6 213 CGLLVIVTYTSILCVLFLCTALI 235 PRIMARY 23 7 264 LIHSVLITLYVSTGVVFSLDMVL 286 SECONDARY 23 8 301 LAANSEVLMMLPRAMLPYLYLLR 323 SECONDARY 23 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9404 Download

>KIEE9404  1041 bp
ATGGGGGATGAGCTGGCACCTTGCCCTGTGGGCACTACAGCTTGGCCGGCCCTGATCCAG
CTCATCAGCAAGACACCCTGCATGCCCCAAGCAGCCAGCAACACTTCCTTGGGCCTGGGG
GACCTCAGGGTGCCCAGCTCCATGCTGTACTGGCTTTTCCTTCCCTCAAGCCTGCTGGCT
GCAGCCACACTGGCTGTCAGCCCCCTGCTGCTGGTGACCATCCTGCGGAACCAACGGCTG
CGACAGGAGCCCCACTACCTGCTCCCGGCTAACATCCTGCTCTCAGACCTGGCCTACATT
CTCCTCCACATGCTCATCTCCTCCAGCAGCCTGGGTGGCTGGGAGCTGGGCCGCATGGCC
TGTGGCATTCTCACTGATGCTGTCTTCGCCGCCTGCACCAGCACCATCCTGTCCTTCACC
GCCATTGTGCTGCACACCTACCTGGCAGTCATCCATCCACTGCGCTACCTCTCCTTCATG
TCCCATGGGGCTGCCTGGAAGGCAGTGGCCCTCATCTGGCTGGTGGCCTGCTGCTTCCCC
ACATTCCTTATTTGGCTCAGCAAGTGGCAGGATGCCCAGCTGGAGGAGCAAGGAGCTTCA
TACATCCTACCACCAAGCATGGGCACCCAGCCGGGATGTGGCCTCCTGGTCATTGTTACC
TACACCTCCATTCTGTGCGTTCTGTTCCTCTGCACAGCTCTCATTGCCAACTGTTTCTGG
AGGATCTATGCAGAGGCCAAGACTTCAGGCATCTGGGGGCAGGGCTATTCCCGGGCCAGG
GGCACCCTGCTGATCCACTCAGTGCTGATCACATTGTACGTGAGCACAGGGGTGGTGTTC
TCCCTGGACATGGTGCTGACCAGGTACCACCACATTGACTCTGGGACTCACACATGGCTC
CTGGCAGCTAACAGTGAGGTACTCATGATGCTTCCCCGTGCCATGCTCCCATACCTGTAC
CTGCTCCGCTACCGGCAGCTGTTGGGCATGGTCCGGGGCCACCTCCCATCCAGGAGGCAC
CAGGCCATCTTTACCATTTCC

ORF Protein

Cloned ORF protein sequence for pFN21AE9404 Download

>KIEE9404  347 aa
MGDELAPCPVGTTAWPALIQLISKTPCMPQAASNTSLGLGDLRVPSSMLYWLFLPSSLLA
AATLAVSPLLLVTILRNQRLRQEPHYLLPANILLSDLAYILLHMLISSSSLGGWELGRMA
CGILTDAVFAACTSTILSFTAIVLHTYLAVIHPLRYLSFMSHGAAWKAVALIWLVACCFP
TFLIWLSKWQDAQLEEQGASYILPPSMGTQPGCGLLVIVTYTSILCVLFLCTALIANCFW
RIYAEAKTSGIWGQGYSRARGTLLIHSVLITLYVSTGVVFSLDMVLTRYHHIDSGTHTWL
LAANSEVLMMLPRAMLPYLYLLRYRQLLGMVRGHLPSRRHQAIFTIS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9404 Download

>pFN21AE9404  5746 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGGGATGAGCTGGCACCTTGCCCTGTGGGCAC
TACAGCTTGGCCGGCCCTGATCCAGCTCATCAGCAAGACACCCTGCATGCCCCAAGCAGC
CAGCAACACTTCCTTGGGCCTGGGGGACCTCAGGGTGCCCAGCTCCATGCTGTACTGGCT
TTTCCTTCCCTCAAGCCTGCTGGCTGCAGCCACACTGGCTGTCAGCCCCCTGCTGCTGGT
GACCATCCTGCGGAACCAACGGCTGCGACAGGAGCCCCACTACCTGCTCCCGGCTAACAT
CCTGCTCTCAGACCTGGCCTACATTCTCCTCCACATGCTCATCTCCTCCAGCAGCCTGGG
TGGCTGGGAGCTGGGCCGCATGGCCTGTGGCATTCTCACTGATGCTGTCTTCGCCGCCTG
CACCAGCACCATCCTGTCCTTCACCGCCATTGTGCTGCACACCTACCTGGCAGTCATCCA
TCCACTGCGCTACCTCTCCTTCATGTCCCATGGGGCTGCCTGGAAGGCAGTGGCCCTCAT
CTGGCTGGTGGCCTGCTGCTTCCCCACATTCCTTATTTGGCTCAGCAAGTGGCAGGATGC
CCAGCTGGAGGAGCAAGGAGCTTCATACATCCTACCACCAAGCATGGGCACCCAGCCGGG
ATGTGGCCTCCTGGTCATTGTTACCTACACCTCCATTCTGTGCGTTCTGTTCCTCTGCAC
AGCTCTCATTGCCAACTGTTTCTGGAGGATCTATGCAGAGGCCAAGACTTCAGGCATCTG
GGGGCAGGGCTATTCCCGGGCCAGGGGCACCCTGCTGATCCACTCAGTGCTGATCACATT
GTACGTGAGCACAGGGGTGGTGTTCTCCCTGGACATGGTGCTGACCAGGTACCACCACAT
TGACTCTGGGACTCACACATGGCTCCTGGCAGCTAACAGTGAGGTACTCATGATGCTTCC
CCGTGCCATGCTCCCATACCTGTACCTGCTCCGCTACCGGCAGCTGTTGGGCATGGTCCG
GGGCCACCTCCCATCCAGGAGGCACCAGGCCATCTTTACCATTTCCGTTTAAACGAATTC
GGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTG
CTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT
AACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAA
ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT
TTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT
ATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAA
TGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTA
ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT
CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA
AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGC
GGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAA
GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGC
CGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT
ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACT
GCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATA
CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTA
TTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT
AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT
AAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA
AATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACC
GACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC
GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACA
AAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC
TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC
CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT
TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTG
CTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTA
TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAA
AAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG
AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCC
TTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAAC
CATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTT
GCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGT
TTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGT
ACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAAC
CGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT