(Circular) (Six-base) MAPSORT of: pFN21AE9404.seq Check: 8806 from: 1 to: 5746 pFN21AE9404 5746 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3556 278 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3089 1937 Size: 1152 4594 Acc65I G'GTAC_C Cuts at: 2868 3067 2868 Size: 199 5547 AclI AA'CG_TT Cuts at: 1563 3873 4246 1563 Size: 2310 373 3063 Fragments arranged by size: 3063 2310 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2733 3809 5193 5461 1632 Size: 1101 1076 1384 268 1917 Fragments arranged by size: 1917 1384 1101 1076 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5549 AflIII A'CryG_T Cuts at: 1203 4645 5622 1203 Size: 3442 977 1327 Fragments arranged by size: 3442 1327 977 AgeI A'CCGG_T Cuts at: 4577 4577 Size: 5746 AhdI GACnn_n'nnGTC Cuts at: 4475 4475 Size: 5746 AleI CACnn'nnGTG Cuts at: 1166 2834 1166 Size: 1668 4078 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5746 AlwNI CAG_nnn'CTG Cuts at: 3489 5061 3489 Size: 1572 4174 ApaLI G'TGCA_C Cuts at: 3803 4959 3803 Size: 1156 4590 ApoI r'AATT_y Cuts at: 1493 3055 3281 3433 5542 1493 Size: 1562 226 152 2109 1697 Fragments arranged by size: 2109 1697 1562 226 152 AseI AT'TA_AT Cuts at: 160 1032 4300 160 Size: 872 3268 1606 Fragments arranged by size: 3268 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 AvaI C'yCGr_G Cuts at: 1870 1943 2775 3071 1870 Size: 73 832 296 4545 Fragments arranged by size: 4545 832 296 73 BamHI G'GATC_C Cuts at: 3076 3443 3076 Size: 367 5379 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2020 2134 2627 2786 2868 Size: 325 217 627 233 114 493 159 82 Cuts at: 2868 3021 3067 4522 618 Size: 153 46 1455 1842 Fragments arranged by size: 1842 1455 627 493 325 233 217 159 153 114 82 46 BanII G_rGCy'C Cuts at: 1391 2256 3065 1391 Size: 865 809 4072 Fragments arranged by size: 4072 865 809 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2380 928 Size: 971 481 4294 Fragments arranged by size: 4294 971 481 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3937 3971 1903 Size: 34 2000 34 3678 Fragments arranged by size: 3678 2000 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3638 4848 3638 Size: 1210 4536 BclI T'GATC_A Cuts at: 1548 2811 1548 Size: 1263 4483 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2034 2195 2340 Size: 107 122 71 631 605 362 161 145 Cuts at: 2340 2504 4357 136 Size: 164 1853 1525 Fragments arranged by size: 1853 1525 631 605 362 164 161 145 122 107 71 BglII A'GATC_T Cuts at: 5741 5741 Size: 5746 BlpI GC'TnA_GC Cuts at: 2561 3163 2561 Size: 602 5144 Bme1580I G_kGCm'C Cuts at: 1790 2039 2139 2630 2789 3807 4963 1790 Size: 249 100 491 159 1018 1156 2573 Fragments arranged by size: 2573 1156 1018 491 249 159 100 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5746 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3477 4435 442 Size: 416 53 83 399 24 2060 958 1753 Fragments arranged by size: 2060 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5746 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2311 2606 2743 4406 1239 Size: 1072 295 137 1663 2579 Fragments arranged by size: 2579 1663 1072 295 137 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2155 3871 4736 5034 2155 Size: 1716 865 298 2867 Fragments arranged by size: 2867 1716 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4409 882 Size: 617 2910 2219 Fragments arranged by size: 2910 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2824 3537 493 Size: 711 1620 713 2702 Fragments arranged by size: 2702 1620 713 711 BsaBI GATnn'nnATC Cuts at: 5740 5740 Size: 5746 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3553 3935 4570 275 Size: 53 83 186 936 2020 382 635 1451 Fragments arranged by size: 2020 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4179 4577 4851 4998 5409 4179 Size: 398 274 147 411 4516 Fragments arranged by size: 4516 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5521 Fragments arranged by size: 5521 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 2314 2603 1186 Size: 1111 17 289 4329 Fragments arranged by size: 4329 1111 289 17 BseYI C'CCAG_C Cuts at: 2138 2343 2350 2580 2631 4949 5445 2138 Size: 205 7 230 51 2318 496 2439 Fragments arranged by size: 2439 2318 496 230 205 51 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2383 2420 2680 1163 Size: 573 647 37 260 4229 Fragments arranged by size: 4229 647 573 260 37 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3196 3957 4985 1540 Size: 275 186 1195 761 1028 2301 Fragments arranged by size: 2301 1195 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2831 3807 3892 4963 2831 Size: 976 85 1071 3614 Fragments arranged by size: 3614 1071 976 85 BsmI GAATG_Cn' Cuts at: 2370 3257 3350 2370 Size: 887 93 4766 Fragments arranged by size: 4766 887 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2139 2256 2630 2789 2831 3065 Size: 399 249 100 117 374 159 42 234 Cuts at: 3065 3807 3892 4963 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 374 249 234 159 117 100 85 42 BspEI T'CCGG_A Cuts at: 5409 5409 Size: 5746 BspHI T'CATG_A Cuts at: 2928 3635 5365 2928 Size: 707 1730 3309 Fragments arranged by size: 3309 1730 707 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2271 2973 3101 844 Size: 1427 702 128 3489 Fragments arranged by size: 3489 1427 702 128 BsrBI CCG'CTC Cuts at: 1394 1823 3633 1394 Size: 429 1810 3507 Fragments arranged by size: 3507 1810 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2706 4241 4415 65 Size: 1183 1458 1535 174 1396 Fragments arranged by size: 1535 1458 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2050 2976 4390 4577 1700 Size: 110 240 926 1414 187 2869 Fragments arranged by size: 2869 1414 926 240 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5746 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5746 BssSI C'ACGA_G Cuts at: 3806 4818 3806 Size: 1012 4734 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5746 BstBI TT'CG_AA Cuts at: 4550 4550 Size: 5746 BstEII G'GTnAC_C Cuts at: 1184 2218 1184 Size: 1034 4712 BstXI CCAn_nnnn'nTGG Cuts at: 2335 2335 Size: 5746 BstYI r'GATC_y Cuts at: 1972 2727 3076 3443 3828 3845 5297 5383 5395 Size: 755 349 367 385 17 1452 86 12 Cuts at: 5395 5741 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 755 385 367 349 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2129 2129 Size: 5746 BtgI C'CryG_G Cuts at: 513 1065 2004 2488 513 Size: 552 939 484 3771 Fragments arranged by size: 3771 939 552 484 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5007 Fragments arranged by size: 5007 683 56 BtsI GCAGTG_nn' Cuts at: 2462 2514 3262 4056 4076 2462 Size: 52 748 794 20 4132 Fragments arranged by size: 4132 794 748 52 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5711 DraI TTT'AAA Cuts at: 3050 3050 Size: 5746 DraIII CAC_nnn'GTG Cuts at: 2806 2806 Size: 5746 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4753 809 Size: 507 3437 1802 Fragments arranged by size: 3437 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2048 3193 4082 Size: 54 1425 50 275 18 218 1145 889 Cuts at: 4082 5711 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1145 889 275 218 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3193 1537 Size: 275 1381 4090 Fragments arranged by size: 4090 1381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3676 1353 Size: 2323 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4333 4707 4853 387 Size: 1064 138 2744 374 146 1280 Fragments arranged by size: 2744 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2311 2606 2733 2743 3809 4406 5193 Size: 393 679 295 127 10 1066 597 787 Cuts at: 5193 5461 1239 Size: 268 1524 Fragments arranged by size: 1524 1066 787 679 597 393 295 268 127 10 EcoO109I rG'GnC_Cy Cuts at: 2125 2125 Size: 5746 EcoRI G'AATT_C Cuts at: 3055 3433 3055 Size: 378 5368 FseI GG_CCGG'CC Cuts at: 2054 2054 Size: 5746 FspI TGC'GCA Cuts at: 4252 4252 Size: 5746 HaeII r_GCGC'y Cuts at: 1678 4893 1678 Size: 3215 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2631 2663 4387 4419 4461 Size: 32 135 32 1059 32 1724 32 42 Cuts at: 4461 4493 1373 Size: 32 2626 Fragments arranged by size: 2626 1724 1059 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3090 3336 669 Size: 988 281 1152 246 3079 Fragments arranged by size: 3079 1152 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5746 HpaI GTT'AAC Cuts at: 3336 3336 Size: 5746 KpnI G_GTAC'C Cuts at: 2872 3071 2872 Size: 199 5547 MfeI C'AATT_G Cuts at: 3345 3345 Size: 5746 MluI A'CGCG_T Cuts at: 5622 5622 Size: 5746 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4860 5044 1305 Size: 3555 184 2007 Fragments arranged by size: 3555 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3924 Fragments arranged by size: 3924 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2834 3704 4063 4222 518 Size: 648 1668 870 359 159 2042 Fragments arranged by size: 2042 1668 870 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2584 2983 3162 3839 4987 5232 1606 Size: 978 399 179 677 1148 245 2120 Fragments arranged by size: 2120 1148 978 677 399 245 179 NaeI GCC'GGC Cuts at: 2052 2052 Size: 5746 NcoI C'CATG_G Cuts at: 513 1065 2004 2488 513 Size: 552 939 484 3771 Fragments arranged by size: 3771 939 552 484 NdeI CA'TA_TG Cuts at: 387 387 Size: 5746 NgoMIV G'CCGG_C Cuts at: 2050 2050 Size: 5746 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5746 NotI GC'GGCC_GC Cuts at: 3193 3193 Size: 5746 NspI r_CATG'y Cuts at: 1132 1229 2089 2317 3104 4649 1132 Size: 97 860 228 787 1545 2229 Fragments arranged by size: 2229 1545 860 787 228 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5746 PciI A'CATG_T Cuts at: 4645 4645 Size: 5746 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5746 PfoI T'CCnGG_A Cuts at: 3014 3014 Size: 5746 PmeI GTTT'AAAC Cuts at: 3050 3050 Size: 5746 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3815 3847 5328 5360 3815 Size: 32 1481 32 4201 Fragments arranged by size: 4201 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2125 2125 Size: 5746 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5746 PsiI TTA'TAA Cuts at: 3316 3316 Size: 5746 PstI C_TGCA'G Cuts at: 830 2188 3098 830 Size: 1358 910 3478 Fragments arranged by size: 3478 1358 910 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5746 PvuII CAG'CTG Cuts at: 2584 2983 2584 Size: 399 5347 SalI G'TCGA_C Cuts at: 1936 3088 1936 Size: 1152 4594 SbfI CC_TGCA'GG Cuts at: 3098 3098 Size: 5746 ScaI AGT'ACT Cuts at: 3994 5706 3994 Size: 1712 4034 SexAI A'CCwGG_T Cuts at: 2864 2864 Size: 5746 SfcI C'TryA_G Cuts at: 826 1045 2040 2184 3094 4229 4910 5101 826 Size: 219 995 144 910 1135 681 191 1471 Fragments arranged by size: 1471 1135 995 910 681 219 191 144 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5746 SmaI CCC'GGG Cuts at: 1872 2777 3073 1872 Size: 905 296 4545 Fragments arranged by size: 4545 905 296 SmlI C'TyrA_G Cuts at: 820 1017 1943 2170 3850 4751 5013 820 Size: 197 926 227 1680 901 262 1553 Fragments arranged by size: 1680 1553 926 901 262 227 197 SnaBI TAC'GTA Cuts at: 493 3537 493 Size: 3044 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5746 SphI G_CATG'C Cuts at: 1132 2089 3104 1132 Size: 957 1015 3774 Fragments arranged by size: 3774 1015 957 SspI AAT'ATT Cuts at: 5 52 3670 5 Size: 47 3618 2081 Fragments arranged by size: 3618 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5746 StyI C'CwwG_G Cuts at: 513 1065 2004 2112 2488 3185 513 Size: 552 939 108 376 697 3074 Fragments arranged by size: 3074 939 697 552 376 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2327 2645 3773 3790 3943 4128 4573 Size: 1255 747 318 1128 17 153 185 445 Cuts at: 4573 325 Size: 1498 Fragments arranged by size: 1498 1255 1128 747 445 318 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2151 3992 5704 Size: 274 80 33 51 1443 174 1841 1712 Cuts at: 5704 96 Size: 138 Fragments arranged by size: 1841 1712 1443 274 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2996 3028 5706 5738 2996 Size: 32 2678 32 3004 Fragments arranged by size: 3004 2678 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5746 XbaI T'CTAG_A Cuts at: 3082 3082 Size: 5746 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2639 2882 2639 Size: 243 5503 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5746 XmaI C'CCGG_G Cuts at: 1870 2775 3071 1870 Size: 905 296 4545 Fragments arranged by size: 4545 905 296 XmnI GAAnn'nnTTC Cuts at: 3875 3875 Size: 5746 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3554 276 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI