Restriction map for
pFN21AE9404
##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BfaI BfrI BglII
BlnI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I ClaI CpoI
DraI DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FauI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI Ksp632I KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MseI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I Van91I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 863 GGTACC G^GTACC
AocI 123 CCTCAGG CC^T[ACGT]AGG
Asp718I 863 GGTACC G^GTACC
AspHI 822 GAGCAC G[AT]GC[AT]^C
AvaI 770 CCCGGG C^[CT]CG[GA]G
BanII 247 GAGCCC G[GA]GC[CT]^C
BbsI 382 GTCTTC GAAGAC(2/6)
BclI 806 TGATCA T^GATCA
BlpI 555 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 555 GCTCAGC GC^T[ACGT]AGC
BpuAI 382 GTCTTC GAAGAC(2/6)
BsaAI 817 TACGTG [CT]AC^GT[GA]
BsiHKAI 822 GAGCAC G[AT]GC[AT]^C
BsmI 365 GCATTC GAATGC(1/-1)
BsoBI 770 CCCGGG C^[CT]CG[GA]G
BspHI 923 TCATGA T^CATGA
BsrDI 702 CATTGC GCAATG(2/0)
BstEII 213 GGTGACC G^GT[ACGT]ACC
BstPI 213 GGTGACC G^GT[ACGT]ACC
BstXI 323 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 722 GGATCT [GA]^GATC[CT]
Bsu36I 123 CCTCAGG CC^T[ACGT]AGG
CelII 555 GCTCAGC GC^T[ACGT]AGC
CfoI 462 GCGC GCG^C
DraII 119 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 796 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 483 CCATGG C^C[GA][CT]GG
EaeI 43 TGGCCG [CT]^GGCC[GA]
Eco81I 123 CCTCAGG CC^T[ACGT]AGG
EcoO109I 119 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 213 GGTGACC G^GT[ACGT]ACC
FbaI 806 TGATCA T^GATCA
FseI 44 GGCCGGCC GGCCGG^CC
HhaI 462 GCGC GCG^C
HinP1I 462 GCGC G^CGC
KpnI 863 GGTACC GGTAC^C
MaeII 818 ACGT A^CGT
MboII 383 TCTTC GAAGA(8/7)
MflI 722 GGATCT [GA]^GATC[CT]
MslI 825 CACAGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 45 GCCGGC GCC^GGC
NcoI 483 CCATGG C^CATGG
NgoMIV 45 GCCGGC G^CCGGC
PpuMI 119 GGGACCT [GA]G^G[AT]CC[CT]
PstI 179 CTGCAG CTGCA^G
RcaI 923 TCATGA T^CATGA
SexAI 859 ACCAGGT A^CC[AT]GGT
SmaI 770 CCCGGG CCC^GGG
SmlI 165 CTCAAG C^T[CT][GA]AG
SphI 80 GCATGC GCATG^C
Tsp45I 214 GTGAC ^GT[GC]AC
XhoII 722 GGATCT [GA]^GATC[CT]
XmaCI 770 CCCGGG C^CCGGG
XmaI 770 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaII 120 GGACC G^G[AT]CC
990 GGTCC
BspMI 257 ACCTGC ACCTGC(4/8)
959 ACCTGC
BsrFI 45 GCCGGC [GA]^CCGG[CT]
971 ACCGGC
BsrI 149 ACTGG ACTGG(1/-1)
189 ACTGG
Cfr10I 45 GCCGGC [GA]^CCGG[CT]
971 ACCGGC
EcoT14I 107 CCTTGG C^C[AT][AT]GG
483 CCATGG
HinfI 877 GACTC G^A[ACGT]TC
885 GACTC
HphI 213 GGTGA GGTGA(8/7)
416 TCACC
MaeIII 214 GTGAC ^GT[ACGT]AC
655 GTTAC
MspA1I 577 CAGCTG C[AC]G^C[GT]G
976 CAGCTG
NspI 80 GCATGC [GA]CATG^[CT]
308 ACATGC
PleI 877 GACTC GAGTC(4/5)
885 GACTC
PvuII 577 CAGCTG CAG^CTG
976 CAGCTG
SfcI 35 CTACAG C^T[GA][CT]AG
179 CTGCAG
StyI 107 CCTTGG C^C[AT][AT]GG
483 CCATGG
XcmI 627 CCAGCCGGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
870 CCACATTGACTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI AlwI BanI
BbvI BcnI BglI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmFI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HpaII
ItaI MboI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TseI TspRI