HBS1L ORF clone FHC14722

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC14722

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Clone info.

Species

human

Clone Name

pFN21AE5782 Sequence (with vector) Restriction Map

Product ID

FHC14722

ORF Sequence

DNA sequence (2052 bp)
Protein sequence (684 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5782

Symbol

HBS1L

Alias : EF-1a, ERFS, HBS1, HSPC276, eRF3c

Description

HBS1-like translational GTPase, transcript variant 1

Original Clone ID

oc95782

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2052 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 684 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5173.1 0 100.0 HBS1L CCDS47479.1 4.1e-215 93.9 HBS1L CCDS14336.1 1.2e-59 37.9 GSPT2 CCDS45414.1 5.1e-59 39.0 GSPT1 CCDS45413.1 6.3e-59 39.0 GSPT1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition BlastProDom IPR015033 1 145 PD278081 Domain of unknown function DUF1916 FPrintScan IPR000795 262 275 PR00315 Protein synthesis factor IPR000795 321 329 PR00315 Protein synthesis factor IPR000795 341 351 PR00315 Protein synthesis factor IPR000795 357 368 PR00315 Protein synthesis factor IPR000795 401 410 PR00315 Protein synthesis factor HMMPfam IPR015033 1 132 PF08938 Domain of unknown function DUF1916 IPR000795 259 476 PF00009 Protein synthesis factor IPR004161 502 569 PF03144 Translation elongation factor EFTu/EF1A IPR004160 574 682 PF03143 Translation elongation factor EFTu/EF1A
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_006611 684 612450 100.0 100.0 NP_001138630 642 612450 93.9 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5782 Download

>KIEE5782  2052 bp
ATGGCCCGGCATCGGAATGTTCGAGGCTATAACTACGATGAAGATTTTGAAGATGATGAT
CTCTACGGCCAGTCTGTAGAGGATGATTATTGTATTTCGCCGTCAACAGCTGCTCAGTTT
ATTTATTCACGGCGTGACAAACCTTCCGTTGAGCCTGTGGAAGAATATGATTATGAAGAT
CTGAAAGAATCTTCCAATTCTGTTTCAAACCATCAGCTCAGTGGATTTGATCAAGCTCGT
CTTTATTCATGCCTTGATCACATGAGAGAGGTACTTGGAGATGCTGTGCCAGATGAAATA
TTAATTGAAGCAGTTCTGAAGAACAAGTTTGATGTGCAGAAGGCTTTGTCAGGGGTTCTG
GAACAAGATAGAGTGCAGAGTTTGAAGGACAAGAATGAGGCAACAGTATCTACAGGAAAG
ATAGCAAAAGGAAAACCAGTAGATTCCCAGACATCGCGAAGTGAATCTGAAATTGTGCCA
AAAGTTGCTAAAATGACTGTATCTGGAAAGAAGCAAACTATGGGATTTGAAGTGCCTGGA
GTATCTTCTGAAGAAAATGGTCATAGTTTCCACACACCTCAAAAAGGACCGCCCATTGAA
GATGCCATTGCTTCTTCCGATGTTCTTGAGACTGCTTCTAAATCTGCTAATCCACCCCAC
ACGATTCAAGCATCAGAAGAGCAGAGTTCAACCCCAGCACCGGTGAAAAAGTCTGGCAAG
CTGAGGCAGCAAATAGATGTGAAGGCGGAACTGGAGAAGCGGCAAGGAGGGAAGCAGCTA
CTCAACTTAGTGGTCATTGGTCATGTTGATGCTGGGAAAAGTACTCTGATGGGCCATATG
CTTTATCTTCTGGGTAATATAAACAAAAGAACTATGCATAAGTATGAACAGGAGTCTAAA
AAGGCTGGCAAAGCTTCGTTTGCATATGCATGGGTCTTGGATGAAACTGGCGAAGAAAGG
GAAAGGGGAGTAACCATGGATGTTGGTATGACAAAGTTTGAAACCACAACCAAAGTTATT
ACATTAATGGATGCTCCAGGCCATAAGGACTTCATTCCAAATATGATTACAGGAGCAGCC
CAGGCGGATGTAGCTGTTTTAGTTGTAGATGCCAGCAGGGGAGAGTTTGAAGCTGGATTT
GAGACTGGAGGACAAACACGAGAGCATGGACTCTTGGTCCGTTCTCTGGGAGTGACGCAG
CTTGCAGTTGCAGTTAATAAAATGGATCAGGTTAATTGGCAACAAGAAAGGTTTCAAGAG
ATTACTGGAAAACTTGGGCACTTTCTTAAGCAAGCAGGTTTTAAGGAGAGTGATGTAGGT
TTTATTCCTACAAGTGGTCTCAGTGGTGAAAATCTAATCACAAGATCTCAGTCAAGTGAA
CTCACAAAATGGTATAAAGGACTATGTTTATTAGAACAAATTGATTCCTTTAAGCCTCCC
CAGCGATCTATTGACAAACCTTTTAGATTATGTGTGTCCGATGTTTTCAAAGATCAAGGA
TCTGGATTTTGCATAACTGGTAAAATAGAAGCTGGTTATATCCAAACTGGTGACCGACTA
CTGGCAATGCCTCCTAATGAAACTTGTACCGTGAAAGGAATCACTCTGCATGATGAACCT
GTCGACTGGGCGGCAGCAGGCGATCATGTTAGTCTTACTTTGGTTGGGATGGATATCATC
AAAATCAATGTTGGCTGCATATTTTGTGGCCCCAAAGTACCCATTAAAGCTTGCACTCGT
TTCAGAGCCCGAATCCTCATCTTTAATATTGAAATTCCTATCACTAAAGGATTTCCTGTG
CTGTTACACTACCAAACTGTCAGTGAACCCGCCGTTATTAAACGATTGATTAGTGTCTTA
AACAAAAGCACGGGTGAAGTCACAAAGAAAAAGCCTAAGTTTTTGACTAAAGGCCAGAAT
GCATTGGTAGAGCTACAGACACAAAGACCAATAGCTCTTGAGCTATATAAAGACTTTAAA
GAGCTGGGGAGGTTCATGCTACGTTACGGTGGTTCTACAATAGCTGCTGGTGTTGTCACT
GAGATAAAAGAA

ORF Protein

Cloned ORF protein sequence for pFN21AE5782 Download

>KIEE5782  684 aa
MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTAAQFIYSRRDKPSVEPVEEYDYED
LKESSNSVSNHQLSGFDQARLYSCLDHMREVLGDAVPDEILIEAVLKNKFDVQKALSGVL
EQDRVQSLKDKNEATVSTGKIAKGKPVDSQTSRSESEIVPKVAKMTVSGKKQTMGFEVPG
VSSEENGHSFHTPQKGPPIEDAIASSDVLETASKSANPPHTIQASEEQSSTPAPVKKSGK
LRQQIDVKAELEKRQGGKQLLNLVVIGHVDAGKSTLMGHMLYLLGNINKRTMHKYEQESK
KAGKASFAYAWVLDETGEERERGVTMDVGMTKFETTTKVITLMDAPGHKDFIPNMITGAA
QADVAVLVVDASRGEFEAGFETGGQTREHGLLVRSLGVTQLAVAVNKMDQVNWQQERFQE
ITGKLGHFLKQAGFKESDVGFIPTSGLSGENLITRSQSSELTKWYKGLCLLEQIDSFKPP
QRSIDKPFRLCVSDVFKDQGSGFCITGKIEAGYIQTGDRLLAMPPNETCTVKGITLHDEP
VDWAAAGDHVSLTLVGMDIIKINVGCIFCGPKVPIKACTRFRARILIFNIEIPITKGFPV
LLHYQTVSEPAVIKRLISVLNKSTGEVTKKKPKFLTKGQNALVELQTQRPIALELYKDFK
ELGRFMLRYGGSTIAAGVVTEIKE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5782 Download

>pFN21AE5782  6757 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCCGGCATCGGAATGTTCGAGGCTATAACTA
CGATGAAGATTTTGAAGATGATGATCTCTACGGCCAGTCTGTAGAGGATGATTATTGTAT
TTCGCCGTCAACAGCTGCTCAGTTTATTTATTCACGGCGTGACAAACCTTCCGTTGAGCC
TGTGGAAGAATATGATTATGAAGATCTGAAAGAATCTTCCAATTCTGTTTCAAACCATCA
GCTCAGTGGATTTGATCAAGCTCGTCTTTATTCATGCCTTGATCACATGAGAGAGGTACT
TGGAGATGCTGTGCCAGATGAAATATTAATTGAAGCAGTTCTGAAGAACAAGTTTGATGT
GCAGAAGGCTTTGTCAGGGGTTCTGGAACAAGATAGAGTGCAGAGTTTGAAGGACAAGAA
TGAGGCAACAGTATCTACAGGAAAGATAGCAAAAGGAAAACCAGTAGATTCCCAGACATC
GCGAAGTGAATCTGAAATTGTGCCAAAAGTTGCTAAAATGACTGTATCTGGAAAGAAGCA
AACTATGGGATTTGAAGTGCCTGGAGTATCTTCTGAAGAAAATGGTCATAGTTTCCACAC
ACCTCAAAAAGGACCGCCCATTGAAGATGCCATTGCTTCTTCCGATGTTCTTGAGACTGC
TTCTAAATCTGCTAATCCACCCCACACGATTCAAGCATCAGAAGAGCAGAGTTCAACCCC
AGCACCGGTGAAAAAGTCTGGCAAGCTGAGGCAGCAAATAGATGTGAAGGCGGAACTGGA
GAAGCGGCAAGGAGGGAAGCAGCTACTCAACTTAGTGGTCATTGGTCATGTTGATGCTGG
GAAAAGTACTCTGATGGGCCATATGCTTTATCTTCTGGGTAATATAAACAAAAGAACTAT
GCATAAGTATGAACAGGAGTCTAAAAAGGCTGGCAAAGCTTCGTTTGCATATGCATGGGT
CTTGGATGAAACTGGCGAAGAAAGGGAAAGGGGAGTAACCATGGATGTTGGTATGACAAA
GTTTGAAACCACAACCAAAGTTATTACATTAATGGATGCTCCAGGCCATAAGGACTTCAT
TCCAAATATGATTACAGGAGCAGCCCAGGCGGATGTAGCTGTTTTAGTTGTAGATGCCAG
CAGGGGAGAGTTTGAAGCTGGATTTGAGACTGGAGGACAAACACGAGAGCATGGACTCTT
GGTCCGTTCTCTGGGAGTGACGCAGCTTGCAGTTGCAGTTAATAAAATGGATCAGGTTAA
TTGGCAACAAGAAAGGTTTCAAGAGATTACTGGAAAACTTGGGCACTTTCTTAAGCAAGC
AGGTTTTAAGGAGAGTGATGTAGGTTTTATTCCTACAAGTGGTCTCAGTGGTGAAAATCT
AATCACAAGATCTCAGTCAAGTGAACTCACAAAATGGTATAAAGGACTATGTTTATTAGA
ACAAATTGATTCCTTTAAGCCTCCCCAGCGATCTATTGACAAACCTTTTAGATTATGTGT
GTCCGATGTTTTCAAAGATCAAGGATCTGGATTTTGCATAACTGGTAAAATAGAAGCTGG
TTATATCCAAACTGGTGACCGACTACTGGCAATGCCTCCTAATGAAACTTGTACCGTGAA
AGGAATCACTCTGCATGATGAACCTGTCGACTGGGCGGCAGCAGGCGATCATGTTAGTCT
TACTTTGGTTGGGATGGATATCATCAAAATCAATGTTGGCTGCATATTTTGTGGCCCCAA
AGTACCCATTAAAGCTTGCACTCGTTTCAGAGCCCGAATCCTCATCTTTAATATTGAAAT
TCCTATCACTAAAGGATTTCCTGTGCTGTTACACTACCAAACTGTCAGTGAACCCGCCGT
TATTAAACGATTGATTAGTGTCTTAAACAAAAGCACGGGTGAAGTCACAAAGAAAAAGCC
TAAGTTTTTGACTAAAGGCCAGAATGCATTGGTAGAGCTACAGACACAAAGACCAATAGC
TCTTGAGCTATATAAAGACTTTAAAGAGCTGGGGAGGTTCATGCTACGTTACGGTGGTTC
TACAATAGCTGCTGGTGTTGTCACTGAGATAAAAGAAGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT