(Linear) (Six-base) MAPSORT of: KIEE5782.seq Check: 3185 from: 1 to: 2052 KIEE5782.orf With 182 enzymes: SgfI * December 5, 2014 13:11 .. AccI GT'mk_AC Cuts at: 0 1622 2052 Size: 1622 430 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 569 2052 Size: 337 232 1483 Fragments arranged by size: 1483 337 232 AflII C'TTAA_G Cuts at: 0 1285 2052 Size: 1285 767 AgeI A'CCGG_T Cuts at: 0 699 2052 Size: 699 1353 AhdI GACnn_n'nnGTC Cuts at: 0 1174 2052 Size: 1174 878 ApoI r'AATT_y Cuts at: 0 1772 2052 Size: 1772 280 AseI AT'TA_AT Cuts at: 0 301 1024 2052 Size: 301 723 1028 Fragments arranged by size: 1028 723 301 BanII G_rGCy'C Cuts at: 0 1749 2052 Size: 1749 303 BbvCI CC'TCA_GC Cuts at: 0 721 2052 Size: 721 1331 BclI T'GATC_A Cuts at: 0 228 255 2052 Size: 228 27 1797 Fragments arranged by size: 1797 228 27 BfrBI ATG'CAT Cuts at: 0 876 928 1921 2052 Size: 876 52 993 131 Fragments arranged by size: 993 876 131 52 BglII A'GATC_T Cuts at: 0 177 1363 2052 Size: 177 1186 689 Fragments arranged by size: 1186 689 177 Bme1580I G_kGCm'C Cuts at: 0 1280 2052 Size: 1280 772 BmrI ACTGGGnnnn_n' Cuts at: 0 1635 2052 Size: 1635 417 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 557 772 1019 1166 2052 Size: 557 215 247 147 886 Fragments arranged by size: 886 557 247 215 147 Bpu10I CC'TnA_GC Cuts at: 0 721 2052 Size: 721 1331 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 646 1978 2052 Size: 646 1332 74 Fragments arranged by size: 1332 646 74 BsaI GGTCTCn'nnnn_ Cuts at: 0 1342 2052 Size: 1342 710 BsaBI GATnn'nnATC Cuts at: 0 56 1672 2052 Size: 56 1616 380 Fragments arranged by size: 1616 380 56 BsaWI w'CCGG_w Cuts at: 0 699 2052 Size: 699 1353 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 269 299 2052 Size: 269 30 1753 Fragments arranged by size: 1753 269 30 BseYI C'CCAG_C Cuts at: 0 693 811 1439 1983 2052 Size: 693 118 628 544 69 Fragments arranged by size: 693 628 544 118 69 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 355 394 2052 Size: 355 39 1658 Fragments arranged by size: 1658 355 39 BsmI GAATG_Cn' Cuts at: 0 1923 2052 Size: 1923 129 Bsp1286I G_dGCh'C Cuts at: 0 1280 1749 2052 Size: 1280 469 303 Fragments arranged by size: 1280 469 303 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1285 2052 Size: 1285 767 BsrDI GCAATG_nn' Cuts at: 0 605 1571 2052 Size: 605 966 481 Fragments arranged by size: 966 605 481 BsrFI r'CCGG_y Cuts at: 0 699 2052 Size: 699 1353 BssSI C'ACGA_G Cuts at: 0 1157 2052 Size: 1157 895 BstEII G'GTnAC_C Cuts at: 0 1549 2052 Size: 1549 503 BstYI r'GATC_y Cuts at: 0 177 1363 1498 2052 Size: 177 1186 135 554 Fragments arranged by size: 1186 554 177 135 BtgI C'CryG_G Cuts at: 0 974 2052 Size: 974 1078 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 437 2052 Size: 437 1615 DraI TTT'AAA Cuts at: 0 1977 2052 Size: 1977 75 DraIII CAC_nnn'GTG Cuts at: 0 133 2052 Size: 133 1919 EaeI y'GGCC_r Cuts at: 0 66 2052 Size: 66 1986 EarI CTCTTCn'nnn_ Cuts at: 0 671 2052 Size: 671 1381 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 760 1099 2052 Size: 760 339 953 Fragments arranged by size: 953 760 339 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 557 569 772 1019 1166 2052 Size: 337 220 12 203 247 147 886 Fragments arranged by size: 886 337 247 220 203 147 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 581 2052 Size: 581 1471 EcoRV GAT'ATC Cuts at: 0 1674 2052 Size: 1674 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 256 2052 Size: 256 1796 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1160 1192 1483 1515 1736 1768 2052 Size: 1160 32 291 32 221 32 284 Fragments arranged by size: 1160 291 284 221 32 32 32 HincII GTy'rAC Cuts at: 0 104 1623 2052 Size: 104 1519 429 Fragments arranged by size: 1519 429 104 HindIII A'AGCT_T Cuts at: 0 911 1727 2052 Size: 911 816 325 Fragments arranged by size: 911 816 325 MslI CAynn'nnrTG Cuts at: 0 14 806 881 927 2052 Size: 14 792 75 46 1125 Fragments arranged by size: 1125 792 75 46 14 MspA1I CmG'CkG Cuts at: 0 109 2052 Size: 109 1943 NcoI C'CATG_G Cuts at: 0 974 2052 Size: 974 1078 NdeI CA'TA_TG Cuts at: 0 836 924 2052 Size: 836 88 1128 Fragments arranged by size: 1128 836 88 NruI TCG'CGA Cuts at: 0 456 2052 Size: 456 1596 NsiI A_TGCA'T Cuts at: 0 878 930 1923 2052 Size: 878 52 993 129 Fragments arranged by size: 993 878 129 52 PvuII CAG'CTG Cuts at: 0 109 2052 Size: 109 1943 SalI G'TCGA_C Cuts at: 0 1621 2052 Size: 1621 431 SapI GCTCTTCn'nnn_ Cuts at: 0 671 2052 Size: 671 1381 ScaI AGT'ACT Cuts at: 0 822 2052 Size: 822 1230 SfcI C'TryA_G Cuts at: 0 74 410 1933 2052 Size: 74 336 1523 119 Fragments arranged by size: 1523 336 119 74 SgrAI Cr'CCGG_yG Cuts at: 0 699 2052 Size: 699 1353 SmlI C'TyrA_G Cuts at: 0 625 1285 1957 2052 Size: 625 660 672 95 Fragments arranged by size: 672 660 625 95 SspI AAT'ATT Cuts at: 0 299 1767 2052 Size: 299 1468 285 Fragments arranged by size: 1468 299 285 StyI C'CwwG_G Cuts at: 0 974 2052 Size: 974 1078 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1568 2052 Size: 1568 484 TatI w'GTAC_w Cuts at: 0 820 2052 Size: 820 1232 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 269 301 515 547 1781 1813 2052 Size: 269 32 214 32 1234 32 239 Fragments arranged by size: 1234 269 239 214 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 217 2052 Size: 217 1835 XmnI GAAnn'nnTTC Cuts at: 0 311 1250 2052 Size: 311 939 802 Fragments arranged by size: 939 802 311 Enzymes that do cut: AccI AcuI AflII AgeI AhdI ApoI AseI BanII BbvCI BclI BfrBI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseYI BsgI BsmI Bsp1286I BspMI BsrDI BsrFI BssSI BstEII BstYI BtgI BtgZI DraI DraIII EaeI EarI EciI Eco57MI EcoNI EcoRV FalI Hin4I HincII HindIII MslI MspA1I NcoI NdeI NruI NsiI PvuII SalI SapI ScaI SfcI SgrAI SmlI SspI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflIII AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI BglI BlpI BmgBI BmtI BplI BsaAI BsaHI BseRI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I BtsI ClaI CspCI DrdI EagI EcoICRI EcoO109I EcoRI FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NgoMIV NheI NotI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SanDI SbfI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI XhoI XmaI ZraI