NET1 ORF clone FHC11951

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC11951

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Clone info.

Species

human

Clone Name

pFN21AB5507 Sequence (with vector) Restriction Map

Product ID

FHC11951

Flexi Clone PID

FXC11951

ORF Sequence

DNA sequence (1788 bp)
Protein sequence (596 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB587387]

Gene

KIBB5507

Symbol

NET1

Alias : ARHGEF8, NET1A

Description

neuroepithelial cell transforming 1, transcript variant 1

Original Clone ID

ee00630b

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1788 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 596 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS41483.1 0 100.0 NET1 CCDS7067.1 1.1e-194 97.5 NET1 CCDS46854.1 1.2e-109 62.5 ARHGEF3 CCDS74948.1 2e-108 64.1 ARHGEF3 CCDS2878.1 1.1e-104 62.3 ARHGEF3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000219 179 354 PF00621 Dbl homology (DH) domain IPR001849 393 500 PF00169 Pleckstrin homology domain HMMSmart IPR000219 178 355 SM00325 Dbl homology (DH) domain IPR001849 387 503 SM00233 Pleckstrin homology domain ProfileScan IPR000219 174 356 PS50010 Dbl homology (DH) domain IPR001849 386 501 PS50003 Pleckstrin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001040625 596 606450 100.0 100.0 NP_005854 542 606450 97.5 88.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5507 Download

>KIBB5507  1788 bp
ATGGAGCCCGAGCTGGCGGCTCAGAAGCAGCCTCGACCGCGGAGGCGAAGCCGCCGGGCC
TCTGGGCTCAGCACGGAGGGAGCGACGGGGCCTTCGGCCGACACCTCCGGGTCGGAGCTG
GACGGGAGATGTTCCCTTCGGAGAGGCAGCTCCTTCACATTCTTAACACCTGGCCCCAAC
TGGGACTTCACTTTGAAAAGAAAACGCAGAGAGAAAGATGATGATGTTGTAAGCCTTAGC
AGCCTTGATCTGAAGGAGCCAAGCAATAAAAGAGTTCGACCTCTGGCTCGTGTCACGTCC
TTGGCAAATTTAATCTCTCCTGTAAGAAATGGAGCTGTCAGACGTTTTGGTCAAACAATA
CAGTCATTTACCCTTCGTGGTGACCACAGATCCCCAGCCTCTGCCCAGAAGTTTTCTAGC
AGGTCAACAGTCCCAACACCCGCCAAGAGAAGGAGCAGTGCACTGTGGTCAGAGATGCTG
GACATCACCATGAAGGAGTCTCTCACCACCAGGGAGATCAGACGGCAGGAGGCAATATAT
GAAATGTCCCGAGGTGAACAGGATTTAATTGAGGATCTCAAACTTGCAAGAAAGGCCTAC
CATGACCCCATGTTAAAGTTGTCCATCATGTCAGAAGAGGAACTCACACATATATTTGGT
GATCTGGACTCTTACATACCTCTGCATGAAGATTTGTTGACAAGAATAGGAGAAGCAACC
AAGCCTGATGGAACAGTGGAGCAGATTGGTCACATTCTCGTGAGCTGGTTACCGCGCTTG
AATGCCTACAGAGGTTACTGTAGTAACCAGCTGGCAGCCAAAGCTCTTCTTGATCAAAAG
AAACAGGATCCAAGAGTCCAAGACTTCCTCCAGCGATGTCTCGAGTCTCCCTTCAGTCGA
AAACTAGATCTTTGGAGTTTCCTAGATATCCCTCGAAGTCGCCTAGTCAAATACCCTTTA
CTGTTAAAAGAAATTCTTAAACACACTCCAAAAGAGCACCCTGATGTTCAGCTTCTGGAG
GATGCTATATTGATAATACAGGGAGTCCTCTCTGATATCAACTTGAAGAAAGGTGAATCC
GAGTGCCAGTATTACATCGACAAGCTGGAGTACCTGGATGAAAAGCAGAGGGACCCCAGA
ATCGAAGCGAGCAAAGTGCTGCTGTGCCATGGGGAGCTGCGGAGCAAGAGTGGACATAAA
CTTTACATTTTCCTGTTTCAAGACATCTTGGTTCTGACTCGGCCCGTCACACGGAACGAA
CGGCACTCTTACCAGGTTTACCGGCAGCCAATCCCAGTCCAAGAGCTAGTCCTAGAAGAC
CTGCAGGATGGAGATGTGAGAATGGGAGGCTCCTTTCGAGGAGCTTTCAGTAACTCAGAG
AAAGCTAAAAATATCTTTAGAATTCGCTTCCATGACCCCTCTCCAGCCCAGTCTCACACT
CTGCAAGCCAATGACGTGTTCCACAAGCAGCAGTGGTTCAACTGTATTCGAGCGGCCATT
GCCCCCTTCCAGTCGGCAGGCAGTCCACCTGAGCTGCAGGGCCTGCCGGAGCTGCACGAA
GAGTGTGAGGGGAACCACCCCTCTGCGAGGAAACTCACAGCCCAGAGGAGGGCATCCACA
GTTTCCAGTGTTACTCAGGTAGAAGTTGATGAAAACGCTTACAGATGTGGCTCTGGCATG
CAGATGGCAGAGGACAGCAAGAGCTTAAAGACACACCAGACACAGCCCGGCATCCGAAGA
GCGAGGGACAAAGCCCTTTCTGGTGGCAAACGGAAAGAGACTTTGGTG

ORF Protein

Cloned ORF protein sequence for pFN21AB5507 Download

>KIBB5507  596 aa
MEPELAAQKQPRPRRRSRRASGLSTEGATGPSADTSGSELDGRCSLRRGSSFTFLTPGPN
WDFTLKRKRREKDDDVVSLSSLDLKEPSNKRVRPLARVTSLANLISPVRNGAVRRFGQTI
QSFTLRGDHRSPASAQKFSSRSTVPTPAKRRSSALWSEMLDITMKESLTTREIRRQEAIY
EMSRGEQDLIEDLKLARKAYHDPMLKLSIMSEEELTHIFGDLDSYIPLHEDLLTRIGEAT
KPDGTVEQIGHILVSWLPRLNAYRGYCSNQLAAKALLDQKKQDPRVQDFLQRCLESPFSR
KLDLWSFLDIPRSRLVKYPLLLKEILKHTPKEHPDVQLLEDAILIIQGVLSDINLKKGES
ECQYYIDKLEYLDEKQRDPRIEASKVLLCHGELRSKSGHKLYIFLFQDILVLTRPVTRNE
RHSYQVYRQPIPVQELVLEDLQDGDVRMGGSFRGAFSNSEKAKNIFRIRFHDPSPAQSHT
LQANDVFHKQQWFNCIRAAIAPFQSAGSPPELQGLPELHEECEGNHPSARKLTAQRRAST
VSSVTQVEVDENAYRCGSGMQMAEDSKSLKTHQTQPGIRRARDKALSGGKRKETLV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5507 Download

>pFN21AB5507  6493 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGCCCGAGCTGGCGGCTCAGAAGCAGCCTCG
ACCGCGGAGGCGAAGCCGCCGGGCCTCTGGGCTCAGCACGGAGGGAGCGACGGGGCCTTC
GGCCGACACCTCCGGGTCGGAGCTGGACGGGAGATGTTCCCTTCGGAGAGGCAGCTCCTT
CACATTCTTAACACCTGGCCCCAACTGGGACTTCACTTTGAAAAGAAAACGCAGAGAGAA
AGATGATGATGTTGTAAGCCTTAGCAGCCTTGATCTGAAGGAGCCAAGCAATAAAAGAGT
TCGACCTCTGGCTCGTGTCACGTCCTTGGCAAATTTAATCTCTCCTGTAAGAAATGGAGC
TGTCAGACGTTTTGGTCAAACAATACAGTCATTTACCCTTCGTGGTGACCACAGATCCCC
AGCCTCTGCCCAGAAGTTTTCTAGCAGGTCAACAGTCCCAACACCCGCCAAGAGAAGGAG
CAGTGCACTGTGGTCAGAGATGCTGGACATCACCATGAAGGAGTCTCTCACCACCAGGGA
GATCAGACGGCAGGAGGCAATATATGAAATGTCCCGAGGTGAACAGGATTTAATTGAGGA
TCTCAAACTTGCAAGAAAGGCCTACCATGACCCCATGTTAAAGTTGTCCATCATGTCAGA
AGAGGAACTCACACATATATTTGGTGATCTGGACTCTTACATACCTCTGCATGAAGATTT
GTTGACAAGAATAGGAGAAGCAACCAAGCCTGATGGAACAGTGGAGCAGATTGGTCACAT
TCTCGTGAGCTGGTTACCGCGCTTGAATGCCTACAGAGGTTACTGTAGTAACCAGCTGGC
AGCCAAAGCTCTTCTTGATCAAAAGAAACAGGATCCAAGAGTCCAAGACTTCCTCCAGCG
ATGTCTCGAGTCTCCCTTCAGTCGAAAACTAGATCTTTGGAGTTTCCTAGATATCCCTCG
AAGTCGCCTAGTCAAATACCCTTTACTGTTAAAAGAAATTCTTAAACACACTCCAAAAGA
GCACCCTGATGTTCAGCTTCTGGAGGATGCTATATTGATAATACAGGGAGTCCTCTCTGA
TATCAACTTGAAGAAAGGTGAATCCGAGTGCCAGTATTACATCGACAAGCTGGAGTACCT
GGATGAAAAGCAGAGGGACCCCAGAATCGAAGCGAGCAAAGTGCTGCTGTGCCATGGGGA
GCTGCGGAGCAAGAGTGGACATAAACTTTACATTTTCCTGTTTCAAGACATCTTGGTTCT
GACTCGGCCCGTCACACGGAACGAACGGCACTCTTACCAGGTTTACCGGCAGCCAATCCC
AGTCCAAGAGCTAGTCCTAGAAGACCTGCAGGATGGAGATGTGAGAATGGGAGGCTCCTT
TCGAGGAGCTTTCAGTAACTCAGAGAAAGCTAAAAATATCTTTAGAATTCGCTTCCATGA
CCCCTCTCCAGCCCAGTCTCACACTCTGCAAGCCAATGACGTGTTCCACAAGCAGCAGTG
GTTCAACTGTATTCGAGCGGCCATTGCCCCCTTCCAGTCGGCAGGCAGTCCACCTGAGCT
GCAGGGCCTGCCGGAGCTGCACGAAGAGTGTGAGGGGAACCACCCCTCTGCGAGGAAACT
CACAGCCCAGAGGAGGGCATCCACAGTTTCCAGTGTTACTCAGGTAGAAGTTGATGAAAA
CGCTTACAGATGTGGCTCTGGCATGCAGATGGCAGAGGACAGCAAGAGCTTAAAGACACA
CCAGACACAGCCCGGCATCCGAAGAGCGAGGGACAAAGCCCTTTCTGGTGGCAAACGGAA
AGAGACTTTGGTGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT