(Circular) (Six-base) MAPSORT of: pFN21AB5507.seq Check: 5271 from: 1 to: 6493 pFN21AB5507 6493 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4303 278 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3836 1937 Size: 1899 4594 Acc65I G'GTAC_C Cuts at: 3814 3814 Size: 6493 AclI AA'CG_TT Cuts at: 1563 4620 4993 1563 Size: 3057 373 3063 Fragments arranged by size: 3063 3057 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2276 2881 4556 5940 6208 1632 Size: 644 605 1675 1384 268 1917 Fragments arranged by size: 1917 1675 1384 644 605 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6296 AflIII A'CryG_T Cuts at: 1203 3459 5392 6369 1203 Size: 2256 1933 977 1327 Fragments arranged by size: 2256 1933 1327 977 AgeI A'CCGG_T Cuts at: 5324 5324 Size: 6493 AhdI GACnn_n'nnGTC Cuts at: 5222 5222 Size: 6493 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6493 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3179 1184 Size: 1995 4498 AlwNI CAG_nnn'CTG Cuts at: 2405 3019 3547 4236 5808 2405 Size: 614 528 689 1572 3090 Fragments arranged by size: 3090 1572 689 614 528 ApaLI G'TGCA_C Cuts at: 2463 4550 5706 2463 Size: 2087 1156 3250 Fragments arranged by size: 3250 2087 1156 ApoI r'AATT_y Cuts at: 1493 2311 2976 3405 3802 4028 4180 6289 1493 Size: 818 665 429 397 226 152 2109 1697 Fragments arranged by size: 2109 1697 818 665 429 397 226 152 AseI AT'TA_AT Cuts at: 160 1032 5047 160 Size: 872 4015 1606 Fragments arranged by size: 4015 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 AvaI C'yCGr_G Cuts at: 1870 1943 2012 2553 2885 3818 1870 Size: 73 69 541 332 933 4545 Fragments arranged by size: 4545 933 541 332 73 69 BamHI G'GATC_C Cuts at: 2851 3823 4190 2851 Size: 972 367 5154 Fragments arranged by size: 5154 972 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3814 5269 618 Size: 325 217 627 2027 1455 1842 Fragments arranged by size: 2027 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2073 3812 1391 Size: 622 60 1739 4072 Fragments arranged by size: 4072 1739 622 60 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3327 928 Size: 971 1428 4094 Fragments arranged by size: 4094 1428 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3142 3176 4684 4718 1903 Size: 34 1205 34 1508 34 3678 Fragments arranged by size: 3678 1508 1205 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4385 5595 4385 Size: 1210 5283 BclI T'GATC_A Cuts at: 1548 2836 1548 Size: 1288 5205 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5104 136 Size: 107 122 71 631 605 3432 1525 Fragments arranged by size: 3432 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2911 6488 2911 Size: 3577 2916 BlpI GC'TnA_GC Cuts at: 2072 3910 2072 Size: 1838 4655 Bme1580I G_kGCm'C Cuts at: 1790 2467 4554 5710 1790 Size: 677 2087 1156 2573 Fragments arranged by size: 2573 2087 1156 677 BmgBI CAC'GTC Cuts at: 1318 2301 3460 1318 Size: 983 1159 4351 Fragments arranged by size: 4351 1159 983 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2194 3293 3427 Size: 416 53 83 399 24 777 1099 134 Cuts at: 3427 4224 5182 442 Size: 797 958 1753 Fragments arranged by size: 1753 1099 958 797 777 416 399 134 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6493 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2858 3041 3131 3411 5153 1239 Size: 1619 183 90 280 1742 2579 Fragments arranged by size: 2579 1742 1619 280 183 90 Bpu10I CC'TnA_GC Cuts at: 2240 3534 2240 Size: 1294 5199 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4618 5483 5781 4618 Size: 865 298 5330 Fragments arranged by size: 5330 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5156 882 Size: 617 3657 2219 Fragments arranged by size: 3657 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4284 493 Size: 711 3080 2702 Fragments arranged by size: 3080 2702 711 BsaBI GATnn'nnATC Cuts at: 6487 6487 Size: 6493 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4300 4682 5317 275 Size: 53 83 186 936 2767 382 635 1451 Fragments arranged by size: 2767 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4926 5324 5598 5745 6156 4926 Size: 398 274 147 411 5263 Fragments arranged by size: 5263 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3178 3208 1178 Size: 30 165 30 1775 30 4463 Fragments arranged by size: 4463 1775 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3378 3625 1186 Size: 2192 247 4054 Fragments arranged by size: 4054 2192 247 BseYI C'CCAG_C Cuts at: 2398 5696 6192 2398 Size: 3298 496 2699 Fragments arranged by size: 3298 2699 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3542 1163 Size: 573 1806 4114 Fragments arranged by size: 4114 1806 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2042 2103 3943 4704 5732 1540 Size: 275 186 41 61 1840 761 1028 2301 Fragments arranged by size: 2301 1840 1028 761 275 186 61 41 BsiHKAI G_wGCw'C Cuts at: 2467 3003 4554 4639 5710 2467 Size: 536 1551 85 1071 3250 Fragments arranged by size: 3250 1551 1071 536 85 BsmI GAATG_Cn' Cuts at: 2791 4004 4097 2791 Size: 1213 93 5187 Fragments arranged by size: 5187 1213 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2073 2467 3003 3812 4554 4639 Size: 399 223 60 394 536 809 742 85 Cuts at: 4639 5710 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 809 742 536 399 394 223 85 60 BspEI T'CCGG_A Cuts at: 6156 6156 Size: 6493 BspHI T'CATG_A Cuts at: 4382 6112 4382 Size: 1730 4763 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2415 3333 3848 844 Size: 1571 918 515 3489 Fragments arranged by size: 3489 1571 918 515 BsrBI CCG'CTC Cuts at: 1394 1823 3497 4380 1394 Size: 429 1674 883 3507 Fragments arranged by size: 3507 1674 883 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3501 4988 5162 65 Size: 1183 2253 1487 174 1396 Fragments arranged by size: 2253 1487 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3285 5137 5324 1700 Size: 110 1475 1852 187 2869 Fragments arranged by size: 2869 1852 1475 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6493 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6493 BssSI C'ACGA_G Cuts at: 2292 2762 4553 5565 2292 Size: 470 1791 1012 3220 Fragments arranged by size: 3220 1791 1012 470 BstAPI GCAn_nnn'nTGC Cuts at: 794 3547 794 Size: 2753 3740 BstBI TT'CG_AA Cuts at: 5297 5297 Size: 6493 BstEII G'GTnAC_C Cuts at: 1184 2384 2772 1184 Size: 1200 388 4905 Fragments arranged by size: 4905 1200 388 BstYI r'GATC_y Cuts at: 1972 2393 2578 2851 2911 3823 4190 4575 4592 Size: 421 185 273 60 912 367 385 17 Cuts at: 4592 6044 6130 6142 6488 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 912 421 385 367 346 273 185 86 60 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2042 3172 513 Size: 552 939 38 1130 3834 Fragments arranged by size: 3834 1130 939 552 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2893 487 Size: 683 56 1667 4087 Fragments arranged by size: 4087 1667 683 56 BtsI GCAGTG_nn' Cuts at: 2467 3482 4009 4803 4823 2467 Size: 1015 527 794 20 4137 Fragments arranged by size: 4137 1015 794 527 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6493 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6458 DraI TTT'AAA Cuts at: 3797 3797 Size: 6493 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2352 5500 809 Size: 507 1036 3148 1802 Fragments arranged by size: 3148 1802 1036 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 3498 3940 Size: 54 1425 50 275 18 270 1398 442 Cuts at: 3940 4829 6458 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1398 889 442 275 270 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2100 3940 1537 Size: 275 288 1840 4090 Fragments arranged by size: 4090 1840 288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2634 2835 3558 3736 4423 1353 Size: 1281 201 723 178 687 3423 Fragments arranged by size: 3423 1281 723 687 201 178 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5080 5454 5600 387 Size: 1064 138 3491 374 146 1280 Fragments arranged by size: 3491 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2276 2858 2881 3041 3131 3411 4556 Size: 393 644 582 23 160 90 280 1145 Cuts at: 4556 5153 5940 6208 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1145 787 644 597 582 393 280 268 160 90 23 EcoNI CCTnn'n_nnAGG Cuts at: 3589 3589 Size: 6493 EcoO109I rG'GnC_Cy Cuts at: 2093 3136 3544 2093 Size: 1043 408 5042 Fragments arranged by size: 5042 1043 408 EcoRI G'AATT_C Cuts at: 3405 3802 4180 3405 Size: 397 378 5718 Fragments arranged by size: 5718 397 378 EcoRV GAT'ATC Cuts at: 2932 3061 2932 Size: 129 6364 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2849 2996 2849 Size: 147 6346 FspI TGC'GCA Cuts at: 4999 4999 Size: 6493 HaeII r_GCGC'y Cuts at: 1678 5640 1678 Size: 3962 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2378 2410 2657 2689 5134 Size: 32 135 32 806 32 247 32 2445 Cuts at: 5134 5166 5208 5240 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2445 806 247 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2430 2703 3837 4083 669 Size: 988 281 492 273 1134 246 3079 Fragments arranged by size: 3079 1134 988 492 281 273 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6493 HpaI GTT'AAC Cuts at: 4083 4083 Size: 6493 KpnI G_GTAC'C Cuts at: 3818 3818 Size: 6493 MfeI C'AATT_G Cuts at: 4092 4092 Size: 6493 MluI A'CGCG_T Cuts at: 6369 6369 Size: 6493 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2097 5607 5791 1305 Size: 792 3510 184 2007 Fragments arranged by size: 3510 2007 792 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4671 Fragments arranged by size: 4671 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2492 2762 3006 3686 4451 4810 4969 Size: 648 1326 270 244 680 765 359 159 Cuts at: 4969 518 Size: 2042 Fragments arranged by size: 2042 1326 765 680 648 359 270 244 159 MspA1I CmG'CkG Cuts at: 1606 2044 2815 3909 4586 5734 5979 1606 Size: 438 771 1094 677 1148 245 2120 Fragments arranged by size: 2120 1148 1094 771 677 438 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3172 513 Size: 552 939 1168 3834 Fragments arranged by size: 3834 1168 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6493 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6493 NotI GC'GGCC_GC Cuts at: 3940 3940 Size: 6493 NspI r_CATG'y Cuts at: 1132 1229 3685 3851 5396 1132 Size: 97 2456 166 1545 2229 Fragments arranged by size: 2456 2229 1545 166 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6493 PciI A'CATG_T Cuts at: 5392 5392 Size: 6493 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6493 PmeI GTTT'AAAC Cuts at: 3797 3797 Size: 6493 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3569 3601 4562 4594 6075 6107 3569 Size: 32 961 32 1481 32 3955 Fragments arranged by size: 3955 1481 961 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3136 3136 Size: 6493 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6493 PsiI TTA'TAA Cuts at: 4063 4063 Size: 6493 PstI C_TGCA'G Cuts at: 830 3330 3543 3845 830 Size: 2500 213 302 3478 Fragments arranged by size: 3478 2500 302 213 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6493 PvuII CAG'CTG Cuts at: 2815 2815 Size: 6493 SacII CC_GC'GG Cuts at: 2045 2045 Size: 6493 SalI G'TCGA_C Cuts at: 1936 3835 1936 Size: 1899 4594 SanDI GG'GwC_CC Cuts at: 3136 3136 Size: 6493 SapI GCTCTTCn'nnn_ Cuts at: 2835 3736 2835 Size: 901 5592 SbfI CC_TGCA'GG Cuts at: 3330 3845 3330 Size: 515 5978 ScaI AGT'ACT Cuts at: 4741 6453 4741 Size: 1712 4781 SexAI A'CCwGG_T Cuts at: 3276 3276 Size: 6493 SfcI C'TryA_G Cuts at: 826 1045 2791 2803 3326 3539 3841 4976 5657 Size: 219 1746 12 523 213 302 1135 681 Cuts at: 5657 5848 826 Size: 191 1471 Fragments arranged by size: 1746 1471 1135 681 523 302 219 213 191 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6493 SmaI CCC'GGG Cuts at: 1872 3820 1872 Size: 1948 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2885 4597 5498 5760 820 Size: 197 926 942 1712 901 262 1553 Fragments arranged by size: 1712 1553 942 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4284 493 Size: 3791 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6493 SphI G_CATG'C Cuts at: 1132 3685 3851 1132 Size: 2553 166 3774 Fragments arranged by size: 3774 2553 166 SspI AAT'ATT Cuts at: 5 52 4417 5 Size: 47 4365 2081 Fragments arranged by size: 4365 2081 47 StuI AGG'CCT Cuts at: 1517 2600 1517 Size: 1083 5410 StyI C'CwwG_G Cuts at: 513 1065 2004 2304 3172 3932 513 Size: 552 939 300 868 760 3074 Fragments arranged by size: 3074 939 868 760 552 300 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4520 4537 4690 4875 5320 325 Size: 1255 2940 17 153 185 445 1498 Fragments arranged by size: 2940 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4739 6451 96 Size: 274 80 33 51 1443 2762 1712 138 Fragments arranged by size: 2762 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3178 3210 6453 6485 3178 Size: 32 3243 32 3186 Fragments arranged by size: 3243 3186 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6493 XbaI T'CTAG_A Cuts at: 3829 3829 Size: 6493 XhoI C'TCGA_G Cuts at: 1943 2885 1943 Size: 942 5551 XmaI C'CCGG_G Cuts at: 1870 3818 1870 Size: 1948 4545 XmnI GAAnn'nnTTC Cuts at: 4622 4622 Size: 6493 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4301 276 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SfiI SfoI SrfI SwaI XcmI