SEC61A1 ORF clone FHC11190

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC11190

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Clone info.

Species

human

Clone Name

pFN21AE0592 Sequence (with vector) Restriction Map

Product ID

FHC11190

Flexi Clone PID

FXC11190

ORF Sequence

DNA sequence (1428 bp)
Protein sequence (476 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528869]

Gene

KIEE0592

Symbol

SEC61A1

Alias : ADTKD5, HNFJ4, HSEC61, SEC61, SEC61A

Description

Sec61 translocon alpha 1 subunit

Original Clone ID

oc01292

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1428 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 476 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3046.1 1.2e-208 100.0 SEC61A1 CCDS7088.1 6.5e-200 93.5 SEC61A2 CCDS44359.1 8.4e-179 89.1 SEC61A2 CCDS44358.1 3.2e-174 93.3 SEC61A2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR019561 40 74 PF10559 Translocon Sec61/SecY IPR002208 75 459 PF00344 SecY protein HMMTigr IPR002208 29 465 TIGR00967 SecY protein
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 1 MAIKFLEVIKPFCVILPEIQKPE 23 SECONDARY 23 2 33 LWTAITLFIFLVCCQIPLFGIMS 55 PRIMARY 23 3 72 NRGTLMELGISPIVTSGLIMQLL 94 SECONDARY 23 4 117 KLFGMIITIGQSIVYVMTGMYG 138 SECONDARY 22 5 145 AGICLLITIQLFVAGLIVLLLDE 167 PRIMARY 23 6 171 KGYGLGSGISLFIATNICETIVW 193 SECONDARY 23 7 239 LPNLMNLIATIFVFAVVIYFQGF 261 PRIMARY 23 8 286 TSNIPIILQSALVSNLYVISQML 308 SECONDARY 23 9 363 VYIVFMLGSCAFFSKTWIEVSGS 385 SECONDARY 23 10 428 IGALSVLADFLGAIGSGTGILLA 450 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_037468 476 609213,617056 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE0592 Download

>KIEE0592  1428 bp
ATGGCAATCAAATTTCTGGAAGTCATCAAGCCCTTCTGTGTCATCCTGCCGGAAATTCAG
AAGCCAGAGAGGAAGATTCAGTTTAAGGAGAAAGTGCTGTGGACCGCTATCACCCTCTTT
ATCTTCTTAGTGTGCTGCCAGATTCCCCTGTTTGGGATCATGTCTTCAGATTCAGCTGAC
CCTTTCTATTGGATGAGAGTGATTCTAGCCTCTAACAGAGGCACATTGATGGAGCTAGGG
ATCTCTCCTATTGTCACGTCTGGCCTTATAATGCAACTCTTGGCTGGCGCCAAGATAATT
GAAGTTGGTGACACCCCAAAAGACCGAGCTCTCTTCAACGGAGCCCAAAAGTTATTTGGC
ATGATCATTACTATCGGCCAGTCTATCGTGTATGTGATGACCGGGATGTATGGGGACCCT
TCTGAAATGGGTGCTGGAATTTGCCTGCTAATCACCATTCAGCTCTTTGTTGCTGGCTTA
ATTGTCCTACTTTTGGATGAACTCCTGCAAAAAGGATATGGCCTTGGCTCTGGTATTTCT
CTCTTCATTGCAACTAACATCTGTGAAACCATCGTATGGAAGGCATTCAGCCCCACTACT
GTCAACACTGGCCGAGGAATGGAATTTGAAGGTGCTATCATCGCACTTTTCCATCTGCTG
GCCACACGCACAGACAAGGTCCGAGCCCTTCGGGAGGCGTTCTACCGCCAGAATCTTCCC
AACCTCATGAATCTCATCGCCACCATCTTTGTCTTTGCAGTGGTCATCTATTTCCAGGGC
TTCCGAGTGGACCTGCCAATCAAGTCGGCCCGCTACCGTGGCCAGTACAACACCTATCCC
ATCAAGCTCTTCTATACGTCCAACATCCCCATCATCCTGCAGTCTGCCCTGGTGTCCAAC
CTTTATGTCATCTCCCAAATGCTCTCAGCTCGCTTCAGTGGCAACTTGCTGGTCAGCCTG
CTGGGCACCTGGTCGGACACGTCTTCTGGGGGCCCAGCACGTGCTTATCCAGTTGGTGGC
CTTTGCTATTACCTGTCCCCTCCAGAATCTTTTGGCTCCGTGTTAGAAGACCCGGTCCAT
GCAGTTGTATACATAGTGTTCATGCTGGGCTCCTGTGCATTCTTCTCCAAAACGTGGATT
GAGGTCTCAGGTTCCTCTGCCAAAGATGTTGCAAAGCAGCTGAAGGAGCAGCAGATGGTG
ATGAGAGGCCACCGAGAGACCTCCATGGTCCATGAACTCAACCGGTACATCCCCACAGCC
GCGGCCTTTGGTGGGCTGTGCATCGGGGCCCTCTCGGTCCTGGCTGACTTCCTAGGCGCC
ATTGGGTCTGGAACCGGGATCCTGCTCGCAGTCACAATCATCTACCAGTACTTTGAGATC
TTCGTTAAGGAGCAAAGCGAGGTTGGCAGCATGGGGGCCCTGCTCTTC

ORF Protein

Cloned ORF protein sequence for pFN21AE0592 Download

>KIEE0592  476 aa
MAIKFLEVIKPFCVILPEIQKPERKIQFKEKVLWTAITLFIFLVCCQIPLFGIMSSDSAD
PFYWMRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVGDTPKDRALFNGAQKLFG
MIITIGQSIVYVMTGMYGDPSEMGAGICLLITIQLFVAGLIVLLLDELLQKGYGLGSGIS
LFIATNICETIVWKAFSPTTVNTGRGMEFEGAIIALFHLLATRTDKVRALREAFYRQNLP
NLMNLIATIFVFAVVIYFQGFRVDLPIKSARYRGQYNTYPIKLFYTSNIPIILQSALVSN
LYVISQMLSARFSGNLLVSLLGTWSDTSSGGPARAYPVGGLCYYLSPPESFGSVLEDPVH
AVVYIVFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRETSMVHELNRYIPTA
AAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQYFEIFVKEQSEVGSMGALLF

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE0592 Download

>pFN21AE0592  6133 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAATCAAATTTCTGGAAGTCATCAAGCCCTT
CTGTGTCATCCTGCCGGAAATTCAGAAGCCAGAGAGGAAGATTCAGTTTAAGGAGAAAGT
GCTGTGGACCGCTATCACCCTCTTTATCTTCTTAGTGTGCTGCCAGATTCCCCTGTTTGG
GATCATGTCTTCAGATTCAGCTGACCCTTTCTATTGGATGAGAGTGATTCTAGCCTCTAA
CAGAGGCACATTGATGGAGCTAGGGATCTCTCCTATTGTCACGTCTGGCCTTATAATGCA
ACTCTTGGCTGGCGCCAAGATAATTGAAGTTGGTGACACCCCAAAAGACCGAGCTCTCTT
CAACGGAGCCCAAAAGTTATTTGGCATGATCATTACTATCGGCCAGTCTATCGTGTATGT
GATGACCGGGATGTATGGGGACCCTTCTGAAATGGGTGCTGGAATTTGCCTGCTAATCAC
CATTCAGCTCTTTGTTGCTGGCTTAATTGTCCTACTTTTGGATGAACTCCTGCAAAAAGG
ATATGGCCTTGGCTCTGGTATTTCTCTCTTCATTGCAACTAACATCTGTGAAACCATCGT
ATGGAAGGCATTCAGCCCCACTACTGTCAACACTGGCCGAGGAATGGAATTTGAAGGTGC
TATCATCGCACTTTTCCATCTGCTGGCCACACGCACAGACAAGGTCCGAGCCCTTCGGGA
GGCGTTCTACCGCCAGAATCTTCCCAACCTCATGAATCTCATCGCCACCATCTTTGTCTT
TGCAGTGGTCATCTATTTCCAGGGCTTCCGAGTGGACCTGCCAATCAAGTCGGCCCGCTA
CCGTGGCCAGTACAACACCTATCCCATCAAGCTCTTCTATACGTCCAACATCCCCATCAT
CCTGCAGTCTGCCCTGGTGTCCAACCTTTATGTCATCTCCCAAATGCTCTCAGCTCGCTT
CAGTGGCAACTTGCTGGTCAGCCTGCTGGGCACCTGGTCGGACACGTCTTCTGGGGGCCC
AGCACGTGCTTATCCAGTTGGTGGCCTTTGCTATTACCTGTCCCCTCCAGAATCTTTTGG
CTCCGTGTTAGAAGACCCGGTCCATGCAGTTGTATACATAGTGTTCATGCTGGGCTCCTG
TGCATTCTTCTCCAAAACGTGGATTGAGGTCTCAGGTTCCTCTGCCAAAGATGTTGCAAA
GCAGCTGAAGGAGCAGCAGATGGTGATGAGAGGCCACCGAGAGACCTCCATGGTCCATGA
ACTCAACCGGTACATCCCCACAGCCGCGGCCTTTGGTGGGCTGTGCATCGGGGCCCTCTC
GGTCCTGGCTGACTTCCTAGGCGCCATTGGGTCTGGAACCGGGATCCTGCTCGCAGTCAC
AATCATCTACCAGTACTTTGAGATCTTCGTTAAGGAGCAAAGCGAGGTTGGCAGCATGGG
GGCCCTGCTCTTCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT