(Circular) (Six-base) MAPSORT of: pFN21AE0592.seq Check: 3486 from: 1 to: 6133 pFN21AE0592 6133 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3943 278 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3093 3476 1937 Size: 1156 383 4594 Fragments arranged by size: 4594 1156 383 Acc65I G'GTAC_C Cuts at: 3454 3454 Size: 6133 AclI AA'CG_TT Cuts at: 1563 4260 4633 1563 Size: 2697 373 3063 Fragments arranged by size: 3063 2697 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2154 2923 3206 4196 5580 5848 1632 Size: 522 769 283 990 1384 268 1917 Fragments arranged by size: 1917 1384 990 769 522 283 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5936 AflIII A'CryG_T Cuts at: 1203 2982 5032 6009 1203 Size: 1779 2050 977 1327 Fragments arranged by size: 2050 1779 1327 977 AgeI A'CCGG_T Cuts at: 3246 4964 3246 Size: 1718 4415 AhdI GACnn_n'nnGTC Cuts at: 4862 4862 Size: 6133 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6133 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6133 AlwNI CAG_nnn'CTG Cuts at: 3876 5448 3876 Size: 1572 4561 ApaI G_GGCC'C Cuts at: 2999 3295 3424 2999 Size: 296 129 5708 Fragments arranged by size: 5708 296 129 ApaLI G'TGCA_C Cuts at: 4190 5346 4190 Size: 1156 4977 ApoI r'AATT_y Cuts at: 1493 2015 2058 2442 2627 3442 3668 3820 5929 Size: 522 43 384 185 815 226 152 2109 Cuts at: 5929 1493 Size: 1697 Fragments arranged by size: 2109 1697 815 522 384 226 185 152 43 AseI AT'TA_AT Cuts at: 160 1032 4687 160 Size: 872 3655 1606 Fragments arranged by size: 3655 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 AvaI C'yCGr_G Cuts at: 1870 1943 3458 1870 Size: 73 1515 4545 Fragments arranged by size: 4545 1515 73 AvrII C'CTAG_G Cuts at: 3316 3316 Size: 6133 BamHI G'GATC_C Cuts at: 3342 3463 3830 3342 Size: 121 367 5645 Fragments arranged by size: 5645 367 121 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2291 2969 3320 3454 4909 Size: 325 217 627 504 678 351 134 1455 Cuts at: 4909 618 Size: 1842 Fragments arranged by size: 1842 1455 678 627 504 351 325 217 134 BanII G_rGCy'C Cuts at: 1391 2335 2350 2692 2999 3116 3295 3424 3452 Size: 944 15 342 307 117 179 129 28 Cuts at: 3452 1391 Size: 4072 Fragments arranged by size: 4072 944 342 307 179 129 117 28 15 BbeI G_GCGC'C Cuts at: 2295 3324 2295 Size: 1029 5104 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2160 2979 3078 928 Size: 971 261 819 99 3983 Fragments arranged by size: 3983 971 819 261 99 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4324 4358 1903 Size: 34 2387 34 3678 Fragments arranged by size: 3678 2387 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4025 5235 4025 Size: 1210 4923 BclI T'GATC_A Cuts at: 1548 2367 1548 Size: 819 5314 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2967 4744 136 Size: 107 122 71 631 605 1295 1777 1525 Fragments arranged by size: 1777 1525 1295 631 605 122 107 71 BglII A'GATC_T Cuts at: 3381 6128 3381 Size: 2747 3386 BlpI GC'TnA_GC Cuts at: 3550 3550 Size: 6133 Bme1580I G_kGCm'C Cuts at: 1790 2972 2999 3295 3424 4194 5350 1790 Size: 1182 27 296 129 770 1156 2573 Fragments arranged by size: 2573 1182 1156 770 296 129 27 BmgBI CAC'GTC Cuts at: 1318 2262 2985 1318 Size: 944 723 4466 Fragments arranged by size: 4466 944 723 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3864 4822 442 Size: 416 53 83 399 24 2447 958 1753 Fragments arranged by size: 2447 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6133 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3030 4793 1239 Size: 1791 1763 2579 Fragments arranged by size: 2579 1791 1763 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4258 5123 5421 4258 Size: 865 298 4970 Fragments arranged by size: 4970 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3154 3215 4796 882 Size: 617 1655 61 1581 2219 Fragments arranged by size: 2219 1655 1581 617 61 BsaAI yAC'GTr Cuts at: 493 1204 3005 3924 493 Size: 711 1801 919 2702 Fragments arranged by size: 2702 1801 919 711 BsaBI GATnn'nnATC Cuts at: 6127 6127 Size: 6133 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2292 3321 3940 4322 Size: 53 83 186 936 759 1029 619 382 Cuts at: 4322 4957 275 Size: 635 1451 Fragments arranged by size: 1451 1029 936 759 635 619 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3246 4566 4964 5238 5385 5796 3246 Size: 1320 398 274 147 411 3583 Fragments arranged by size: 3583 1320 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5908 Fragments arranged by size: 5908 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6133 BseYI C'CCAG_C Cuts at: 2965 2998 3109 5336 5832 2965 Size: 33 111 2227 496 3266 Fragments arranged by size: 3266 2227 496 111 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5560 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3583 4344 5372 1540 Size: 275 186 1582 761 1028 2301 Fragments arranged by size: 2301 1582 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2335 4194 4279 5350 2335 Size: 1859 85 1071 3118 Fragments arranged by size: 3118 1859 1071 85 BsmI GAATG_Cn' Cuts at: 2588 3122 3644 3737 2588 Size: 534 522 93 4984 Fragments arranged by size: 4984 534 522 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2335 2350 2692 2972 2999 3116 3295 Size: 399 545 15 342 280 27 117 179 Cuts at: 3295 3424 3452 4194 4279 5350 1391 Size: 129 28 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 545 399 342 280 179 129 117 85 28 27 15 BspEI T'CCGG_A Cuts at: 5796 5796 Size: 6133 BspHI T'CATG_A Cuts at: 2730 4022 5752 2730 Size: 1292 1730 3111 Fragments arranged by size: 3111 1730 1292 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2805 3488 844 Size: 1961 683 3489 Fragments arranged by size: 3489 1961 683 BsrBI CCG'CTC Cuts at: 1394 1823 4020 1394 Size: 429 2197 3507 Fragments arranged by size: 3507 2197 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2550 4628 4802 65 Size: 1183 1302 2078 174 1396 Fragments arranged by size: 2078 1396 1302 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3246 4777 4964 1700 Size: 110 1436 1531 187 2869 Fragments arranged by size: 2869 1531 1436 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6133 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6133 BssSI C'ACGA_G Cuts at: 4193 5205 4193 Size: 1012 5121 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6133 BstBI TT'CG_AA Cuts at: 4937 4937 Size: 6133 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6133 BstYI r'GATC_y Cuts at: 1972 2244 3342 3381 3463 3830 4215 4232 5684 Size: 272 1098 39 82 367 385 17 1452 Cuts at: 5684 5770 5782 6128 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1098 385 367 346 272 86 82 39 17 12 BstZ17I GTA'TAC Cuts at: 3094 3094 Size: 6133 BtgI C'CryG_G Cuts at: 513 1065 2004 2821 3228 3264 513 Size: 552 939 817 407 36 3382 Fragments arranged by size: 3382 939 817 552 407 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2629 2725 487 Size: 683 56 1403 96 3895 Fragments arranged by size: 3895 1403 683 96 56 BtsI GCAGTG_nn' Cuts at: 2769 3649 4443 4463 2769 Size: 880 794 20 4439 Fragments arranged by size: 4439 880 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6133 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2734 2769 529 Size: 35 2170 35 3893 Fragments arranged by size: 3893 2170 35 35 DraI TTT'AAA Cuts at: 3437 3437 Size: 6133 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5140 809 Size: 507 3824 1802 Fragments arranged by size: 3824 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2380 2614 2664 Size: 54 1425 50 275 18 550 234 50 Cuts at: 2664 2824 3580 4469 6098 8 Size: 160 756 889 1629 43 Fragments arranged by size: 1629 1425 889 756 550 275 234 160 54 50 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3580 1537 Size: 275 1768 4090 Fragments arranged by size: 4090 1768 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2342 2552 2858 3434 4063 1353 Size: 989 210 306 576 629 3423 Fragments arranged by size: 3423 989 629 576 306 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4720 5094 5240 387 Size: 1064 138 3131 374 146 1280 Fragments arranged by size: 3131 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2333 2333 Size: 6133 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2154 2923 3030 3206 4196 4793 5580 Size: 393 522 769 107 176 990 597 787 Cuts at: 5580 5848 1239 Size: 268 1524 Fragments arranged by size: 1524 990 787 769 597 522 393 268 176 107 EcoNI CCTnn'n_nnAGG Cuts at: 2696 2696 Size: 6133 EcoO109I rG'GnC_Cy Cuts at: 2419 2995 3291 3292 3420 3421 2419 Size: 576 296 1 128 1 5131 Fragments arranged by size: 5131 576 296 128 1 1 EcoRI G'AATT_C Cuts at: 3442 3820 3442 Size: 378 5755 FspI TGC'GCA Cuts at: 4639 4639 Size: 6133 HaeII r_GCGC'y Cuts at: 1678 2295 3324 5280 1678 Size: 617 1029 1956 2531 Fragments arranged by size: 2531 1956 1029 617 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4774 4806 4848 4880 1373 Size: 32 135 32 3202 32 42 32 2626 Fragments arranged by size: 3202 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2608 3477 3723 669 Size: 988 281 670 869 246 3079 Fragments arranged by size: 3079 988 869 670 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6133 HpaI GTT'AAC Cuts at: 3723 3723 Size: 6133 KasI G'GCGC_C Cuts at: 2291 3320 2291 Size: 1029 5104 KpnI G_GTAC'C Cuts at: 3458 3458 Size: 6133 MfeI C'AATT_G Cuts at: 3732 3732 Size: 6133 MluI A'CGCG_T Cuts at: 6009 6009 Size: 6133 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2889 2925 2958 5247 5431 1305 Size: 1584 36 33 2289 184 2007 Fragments arranged by size: 2289 2007 1584 184 36 33 MscI TGG'CCA Cuts at: 10 64 1489 1832 2666 2826 10 Size: 54 1425 343 834 160 3317 Fragments arranged by size: 3317 1425 834 343 160 54 MslI CAynn'nnrTG Cuts at: 518 1166 2231 4091 4450 4609 518 Size: 648 1065 1860 359 159 2042 Fragments arranged by size: 2042 1860 1065 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2180 3184 3266 3549 4226 5374 5619 1606 Size: 574 1004 82 283 677 1148 245 2120 Fragments arranged by size: 2120 1148 1004 677 574 283 245 82 NarI GG'CG_CC Cuts at: 2292 3321 2292 Size: 1029 5104 NcoI C'CATG_G Cuts at: 513 1065 2004 3228 513 Size: 552 939 1224 3418 Fragments arranged by size: 3418 1224 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6133 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6133 NotI GC'GGCC_GC Cuts at: 3580 3580 Size: 6133 NspI r_CATG'y Cuts at: 1132 1229 3491 5036 1132 Size: 97 2262 1545 2229 Fragments arranged by size: 2262 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6133 PciI A'CATG_T Cuts at: 5032 5032 Size: 6133 PflMI CCAn_nnn'nTGG Cuts at: 1108 2580 2662 3020 3138 1108 Size: 1472 82 358 118 4103 Fragments arranged by size: 4103 1472 358 118 82 PmeI GTTT'AAAC Cuts at: 3437 3437 Size: 6133 PmlI CAC'GTG Cuts at: 3005 3005 Size: 6133 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4202 4234 5715 5747 4202 Size: 32 1481 32 4588 Fragments arranged by size: 4588 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2419 2419 Size: 6133 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6133 PsiI TTA'TAA Cuts at: 2273 3703 2273 Size: 1430 4703 PspOMI G'GGCC_C Cuts at: 2995 3291 3420 2995 Size: 296 129 5708 Fragments arranged by size: 5708 296 129 PstI C_TGCA'G Cuts at: 830 2886 3485 830 Size: 2056 599 3478 Fragments arranged by size: 3478 2056 599 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6133 PvuII CAG'CTG Cuts at: 2180 3184 2180 Size: 1004 5129 SacI G_AGCT'C Cuts at: 2335 2335 Size: 6133 SacII CC_GC'GG Cuts at: 3267 3267 Size: 6133 SalI G'TCGA_C Cuts at: 1936 3475 1936 Size: 1539 4594 SanDI GG'GwC_CC Cuts at: 2419 2419 Size: 6133 SapI GCTCTTCn'nnn_ Cuts at: 2858 3434 2858 Size: 576 5557 SbfI CC_TGCA'GG Cuts at: 3485 3485 Size: 6133 ScaI AGT'ACT Cuts at: 3374 4381 6093 3374 Size: 1007 1712 3414 Fragments arranged by size: 3414 1712 1007 SexAI A'CCwGG_T Cuts at: 2972 2972 Size: 6133 SfcI C'TryA_G Cuts at: 826 1045 2882 3481 4616 5297 5488 826 Size: 219 1837 599 1135 681 191 1471 Fragments arranged by size: 1837 1471 1135 681 599 219 191 SfoI GGC'GCC Cuts at: 2293 3322 2293 Size: 1029 5104 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6133 SmaI CCC'GGG Cuts at: 1872 3460 1872 Size: 1588 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4237 5138 5400 820 Size: 197 926 2294 901 262 1553 Fragments arranged by size: 2294 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3924 493 Size: 3431 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6133 SphI G_CATG'C Cuts at: 1132 3491 1132 Size: 2359 3774 SspI AAT'ATT Cuts at: 5 52 4057 5 Size: 47 4005 2081 Fragments arranged by size: 4005 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6133 StyI C'CwwG_G Cuts at: 513 1065 2004 2527 3228 3316 3572 513 Size: 552 939 523 701 88 256 3074 Fragments arranged by size: 3074 939 701 552 523 256 88 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2343 2423 3289 4160 4177 4330 4515 Size: 1255 763 80 866 871 17 153 185 Cuts at: 4515 4960 325 Size: 445 1498 Fragments arranged by size: 1498 1255 871 866 763 445 185 153 80 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2829 3372 4379 Size: 274 80 33 51 1443 852 543 1007 Cuts at: 4379 6091 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1007 852 543 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6093 6125 6093 Size: 32 6101 Tth111I GACn'n_nGTC Cuts at: 1316 2681 3077 1316 Size: 1365 396 4372 Fragments arranged by size: 4372 1365 396 XbaI T'CTAG_A Cuts at: 3469 3469 Size: 6133 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2357 2357 Size: 6133 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6133 XmaI C'CCGG_G Cuts at: 1870 3458 1870 Size: 1588 4545 XmnI GAAnn'nnTTC Cuts at: 2588 4262 2588 Size: 1674 4459 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3941 276 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SfiI SrfI SwaI