Restriction map for
pFN21AE0592
(Linear) (Six-base) MAPSORT of: KIEE0592.seq Check: 6333 from: 1 to: 1428
KIEE0592.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 0 1088 1428
Size: 1088 340
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 918 1201 1428
Size: 149 769 283 227
Fragments arranged by size:
769 283 227 149
AflIII A'CryG_T
Cuts at: 0 977 1428
Size: 977 451
AgeI A'CCGG_T
Cuts at: 0 1241 1428
Size: 1241 187
ApaI G_GGCC'C
Cuts at: 0 994 1290 1419 1428
Size: 994 296 129 9
Fragments arranged by size:
994 296 129 9
ApoI r'AATT_y
Cuts at: 0 10 53 437 622 1428
Size: 10 43 384 185 806
Fragments arranged by size:
806 384 185 43 10
AvrII C'CTAG_G
Cuts at: 0 1311 1428
Size: 1311 117
BamHI G'GATC_C
Cuts at: 0 1337 1428
Size: 1337 91
BanI G'GyrC_C
Cuts at: 0 286 964 1315 1428
Size: 286 678 351 113
Fragments arranged by size:
678 351 286 113
BanII G_rGCy'C
Cuts at: 0 330 345 687 994 1111 1290 1419 1428
Size: 330 15 342 307 117 179 129 9
Fragments arranged by size:
342 330 307 179 129 117 15 9
BbeI G_GCGC'C
Cuts at: 0 290 1319 1428
Size: 290 1029 109
Fragments arranged by size:
1029 290 109
BbsI GAAGACnn'nnnn_
Cuts at: 0 155 974 1073 1428
Size: 155 819 99 355
Fragments arranged by size:
819 355 155 99
BclI T'GATC_A
Cuts at: 0 362 1428
Size: 362 1066
BglI GCCn_nnn'nGGC
Cuts at: 0 962 1428
Size: 962 466
BglII A'GATC_T
Cuts at: 0 1376 1428
Size: 1376 52
Bme1580I G_kGCm'C
Cuts at: 0 967 994 1290 1419 1428
Size: 967 27 296 129 9
Fragments arranged by size:
967 296 129 27 9
BmgBI CAC'GTC
Cuts at: 0 257 980 1428
Size: 257 723 448
Fragments arranged by size:
723 448 257
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1025 1428
Size: 1025 403
BsaI GGTCTCn'nnnn_
Cuts at: 0 1149 1210 1428
Size: 1149 61 218
Fragments arranged by size:
1149 218 61
BsaAI yAC'GTr
Cuts at: 0 1000 1428
Size: 1000 428
BsaHI Gr'CG_yC
Cuts at: 0 287 1316 1428
Size: 287 1029 112
Fragments arranged by size:
1029 287 112
BsaWI w'CCGG_w
Cuts at: 0 1241 1428
Size: 1241 187
BseYI C'CCAG_C
Cuts at: 0 960 993 1104 1428
Size: 960 33 111 324
Fragments arranged by size:
960 324 111 33
BsiHKAI G_wGCw'C
Cuts at: 0 330 1428
Size: 330 1098
BsmI GAATG_Cn'
Cuts at: 0 583 1117 1428
Size: 583 534 311
Fragments arranged by size:
583 534 311
Bsp1286I G_dGCh'C
Cuts at: 0 330 345 687 967 994 1111 1290 1419
Size: 330 15 342 280 27 117 179 129
Cuts at: 1419 1428
Size: 9
Fragments arranged by size:
342 330 280 179 129 117 27 15
9
BspHI T'CATG_A
Cuts at: 0 725 1428
Size: 725 703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 800 1428
Size: 800 628
BsrDI GCAATG_nn'
Cuts at: 0 545 1428
Size: 545 883
BsrFI r'CCGG_y
Cuts at: 0 1241 1428
Size: 1241 187
BstYI r'GATC_y
Cuts at: 0 239 1337 1376 1428
Size: 239 1098 39 52
Fragments arranged by size:
1098 239 52 39
BstZ17I GTA'TAC
Cuts at: 0 1089 1428
Size: 1089 339
BtgI C'CryG_G
Cuts at: 0 816 1223 1259 1428
Size: 816 407 36 169
Fragments arranged by size:
816 407 169 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 624 720 1428
Size: 624 96 708
Fragments arranged by size:
708 624 96
BtsI GCAGTG_nn'
Cuts at: 0 764 1428
Size: 764 664
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 729 764 1428
Size: 729 35 664
Fragments arranged by size:
729 664 35
EaeI y'GGCC_r
Cuts at: 0 375 609 659 819 1428
Size: 375 234 50 160 609
Fragments arranged by size:
609 375 234 160 50
EarI CTCTTCn'nnn_
Cuts at: 0 337 547 853 1428
Size: 337 210 306 575
Fragments arranged by size:
575 337 306 210
EcoICRI GAG'CTC
Cuts at: 0 328 1428
Size: 328 1100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 918 1025 1201 1428
Size: 149 769 107 176 227
Fragments arranged by size:
769 227 176 149 107
EcoNI CCTnn'n_nnAGG
Cuts at: 0 691 1428
Size: 691 737
EcoO109I rG'GnC_Cy
Cuts at: 0 414 990 1286 1287 1415 1416 1428
Size: 414 576 296 1 128 1 12
Fragments arranged by size:
576 414 296 128 12 1 1
HaeII r_GCGC'y
Cuts at: 0 290 1319 1428
Size: 290 1029 109
Fragments arranged by size:
1029 290 109
HincII GTy'rAC
Cuts at: 0 603 1428
Size: 603 825
KasI G'GCGC_C
Cuts at: 0 286 1315 1428
Size: 286 1029 113
Fragments arranged by size:
1029 286 113
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 884 920 953 1428
Size: 884 36 33 475
Fragments arranged by size:
884 475 36 33
MscI TGG'CCA
Cuts at: 0 661 821 1428
Size: 661 160 607
Fragments arranged by size:
661 607 160
MslI CAynn'nnrTG
Cuts at: 0 226 1428
Size: 226 1202
MspA1I CmG'CkG
Cuts at: 0 175 1179 1261 1428
Size: 175 1004 82 167
Fragments arranged by size:
1004 175 167 82
NarI GG'CG_CC
Cuts at: 0 287 1316 1428
Size: 287 1029 112
Fragments arranged by size:
1029 287 112
NcoI C'CATG_G
Cuts at: 0 1223 1428
Size: 1223 205
PflMI CCAn_nnn'nTGG
Cuts at: 0 575 657 1015 1133 1428
Size: 575 82 358 118 295
Fragments arranged by size:
575 358 295 118 82
PmlI CAC'GTG
Cuts at: 0 1000 1428
Size: 1000 428
PpuMI rG'GwC_Cy
Cuts at: 0 414 1428
Size: 414 1014
PsiI TTA'TAA
Cuts at: 0 268 1428
Size: 268 1160
PspOMI G'GGCC_C
Cuts at: 0 990 1286 1415 1428
Size: 990 296 129 13
Fragments arranged by size:
990 296 129 13
PstI C_TGCA'G
Cuts at: 0 881 1428
Size: 881 547
PvuII CAG'CTG
Cuts at: 0 175 1179 1428
Size: 175 1004 249
Fragments arranged by size:
1004 249 175
SacI G_AGCT'C
Cuts at: 0 330 1428
Size: 330 1098
SacII CC_GC'GG
Cuts at: 0 1262 1428
Size: 1262 166
SanDI GG'GwC_CC
Cuts at: 0 414 1428
Size: 414 1014
SapI GCTCTTCn'nnn_
Cuts at: 0 853 1428
Size: 853 575
ScaI AGT'ACT
Cuts at: 0 1369 1428
Size: 1369 59
SexAI A'CCwGG_T
Cuts at: 0 967 1428
Size: 967 461
SfcI C'TryA_G
Cuts at: 0 877 1428
Size: 877 551
SfoI GGC'GCC
Cuts at: 0 288 1317 1428
Size: 288 1029 111
Fragments arranged by size:
1029 288 111
StyI C'CwwG_G
Cuts at: 0 522 1223 1311 1428
Size: 522 701 88 117
Fragments arranged by size:
701 522 117 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 338 418 1284 1428
Size: 338 80 866 144
Fragments arranged by size:
866 338 144 80
TatI w'GTAC_w
Cuts at: 0 824 1367 1428
Size: 824 543 61
Fragments arranged by size:
824 543 61
Tth111I GACn'n_nGTC
Cuts at: 0 676 1072 1428
Size: 676 396 356
Fragments arranged by size:
676 396 356
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 352 1428
Size: 352 1076
XmnI GAAnn'nnTTC
Cuts at: 0 583 1428
Size: 583 845
Enzymes that do cut:
AccI AcuI AflIII AgeI ApaI ApoI AvrII BamHI
BanI BanII BbeI BbsI BclI BglI BglII Bme1580I
BmgBI BpmI BsaI BsaAI BsaHI BsaWI BseYI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BstYI BstZ17I
BtgI BtgZI BtsI CspCI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I HaeII HincII KasI MmeI MscI MslI
MspA1I NarI NcoI PflMI PmlI PpuMI PsiI PspOMI
PstI PvuII SacI SacII SanDI SapI ScaI SexAI
SfcI SfoI StyI TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AhdI AleI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvaI
BaeI BbvCI BcgI BciVI BfrBI BlpI BmrI BmtI
BplI Bpu10I BpuEI BsaBI BsaXI BseRI BsgI BsiEI
BsiWI BsmBI BspEI BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI Bsu36I ClaI DraI DraIII DrdI
EagI EciI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HindIII HpaI KpnI MfeI MluI NaeI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PpiI PshAI PspXI PsrI PvuI
RsrII SalI SbfI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
XbaI XhoI XmaI ZraI