GTF2B ORF clone FHC10598

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Halotag ORF Clone FHC10598

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Clone info.

Species

human

Clone Name

pFN21AB8232 Sequence (with vector) Restriction Map

Product ID

FHC10598

Flexi Clone PID

FXC10598

ORF Sequence

DNA sequence (948 bp)
Protein sequence (316 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB587461]

Gene

KIBB8232

Symbol

GTF2B

Alias : TF2B, TFIIB

Description

general transcription factor IIB

Original Clone ID

cp00592

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 948 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 316 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS715.1 1.3e-145 100.0 GTF2B CCDS10001.1 2.4e-11 23.8 BRF1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000812 27 47 PR00685 Transcription factor TFIIB IPR000812 49 62 PR00685 Transcription factor TFIIB IPR000812 63 84 PR00685 Transcription factor TFIIB IPR000812 161 180 PR00685 Transcription factor TFIIB IPR000812 187 202 PR00685 Transcription factor TFIIB IPR000812 214 232 PR00685 Transcription factor TFIIB IPR000812 248 264 PR00685 Transcription factor TFIIB IPR000812 280 294 PR00685 Transcription factor TFIIB HMMPfam IPR013137 14 55 PF08271 Zinc finger IPR013150 121 191 PF00382 Transcription factor TFIIB IPR013150 215 285 PF00382 Transcription factor TFIIB HMMSmart IPR013763 119 200 SM00385 Cyclin-like IPR013763 213 294 SM00385 Cyclin-like ProfileScan IPR013137 11 42 PS51134 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001505 316 189963 100.0 100.0 XP_011539601 285 189963 100.0 90.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB8232 Download

>KIBB8232  948 bp
ATGGCGTCTACCAGCCGTTTGGATGCTCTTCCAAGAGTCACATGTCCAAACCATCCAGAT
GCGATTTTAGTGGAGGACTACAGAGCCGGTGATATGATCTGTCCTGAATGTGGCTTGGTT
GTAGGTGACCGGGTTATTGATGTGGGATCTGAATGGCGAACTTTCAGCAATGACAAAGCA
ACAAAAGATCCATCTCGAGTTGGAGATTCTCAGAATCCTCTTCTGAGTGATGGAGATTTG
TCTACCATGATTGGCAAGGGCACAGGAGCTGCAAGTTTTGACGAATTTGGCAATTCTAAG
TACCAGAATCGGAGAACAATGAGCAGTTCTGATCGGGCAATGATGAATGCATTCAAAGAA
ATCACTACCATGGCAGACAGAATCAATCTACCTCGAAATATAGTTGATCGAACAAATAAT
TTATTCAAGCAAGTATATGAACAGAAGAGCCTGAAGGGAAGAGCTAATGATGCTATAGCT
TCTGCTTGTCTCTATATTGCCTGTAGACAAGAAGGGGTTCCTAGGACATTTAAAGAAATA
TGTGCCGTATCACGAATTTCTAAGAAAGAAATTGGTCGGTGTTTTAAACTTATTTTGAAA
GCGCTAGAAACCAGTGTGGATTTGATTACAACTGGGGACTTCATGTCCAGGTTCTGTTCC
AACCTTTGTCTTCCTAAACAAGTACAGATGGCAGCTACACATATAGCCCGTAAAGCTGTG
GAATTGGACTTGGTTCCTGGGAGGAGCCCCATCTCTGTGGCAGCGGCAGCTATTTACATG
GCCTCACAGGCATCAGCTGAAAAGAGGACCCAAAAAGAAATTGGAGATATTGCTGGTGTT
GCTGATGTTACAATCAGACAGTCCTATAGACTGATCTATCCTCGAGCCCCAGATCTGTTT
CCTACAGACTTCAAATTTGACACCCCAGTGGACAAACTACCACAGCTA

ORF Protein

Cloned ORF protein sequence for pFN21AB8232 Download

>KIBB8232  316 aa
MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKA
TKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKE
ITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEI
CAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAV
ELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLF
PTDFKFDTPVDKLPQL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB8232 Download

>pFN21AB8232  5653 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCGTCTACCAGCCGTTTGGATGCTCTTCCAAG
AGTCACATGTCCAAACCATCCAGATGCGATTTTAGTGGAGGACTACAGAGCCGGTGATAT
GATCTGTCCTGAATGTGGCTTGGTTGTAGGTGACCGGGTTATTGATGTGGGATCTGAATG
GCGAACTTTCAGCAATGACAAAGCAACAAAAGATCCATCTCGAGTTGGAGATTCTCAGAA
TCCTCTTCTGAGTGATGGAGATTTGTCTACCATGATTGGCAAGGGCACAGGAGCTGCAAG
TTTTGACGAATTTGGCAATTCTAAGTACCAGAATCGGAGAACAATGAGCAGTTCTGATCG
GGCAATGATGAATGCATTCAAAGAAATCACTACCATGGCAGACAGAATCAATCTACCTCG
AAATATAGTTGATCGAACAAATAATTTATTCAAGCAAGTATATGAACAGAAGAGCCTGAA
GGGAAGAGCTAATGATGCTATAGCTTCTGCTTGTCTCTATATTGCCTGTAGACAAGAAGG
GGTTCCTAGGACATTTAAAGAAATATGTGCCGTATCACGAATTTCTAAGAAAGAAATTGG
TCGGTGTTTTAAACTTATTTTGAAAGCGCTAGAAACCAGTGTGGATTTGATTACAACTGG
GGACTTCATGTCCAGGTTCTGTTCCAACCTTTGTCTTCCTAAACAAGTACAGATGGCAGC
TACACATATAGCCCGTAAAGCTGTGGAATTGGACTTGGTTCCTGGGAGGAGCCCCATCTC
TGTGGCAGCGGCAGCTATTTACATGGCCTCACAGGCATCAGCTGAAAAGAGGACCCAAAA
AGAAATTGGAGATATTGCTGGTGTTGCTGATGTTACAATCAGACAGTCCTATAGACTGAT
CTATCCTCGAGCCCCAGATCTGTTTCCTACAGACTTCAAATTTGACACCCCAGTGGACAA
ACTACCACAGCTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT