Restriction map for
pFN21AB8232
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspHI AspI AviII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I Cac8I CelII ClaI CpoI
DraIII DrdI EaeI EagI EclXI
Eco52I Eco81I EcoNI EcoRI EcoRV
FauI FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AciI 763 GCGG CCGC(-3/-1)
AcyI 3 GGCGTC G[GA]^CG[CT]C
AflIII 40 ACATGT A^C[GA][CT]GT
AhdI 637 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 600 AGCGCT AGC^GCT
AspEI 637 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 806 GGACC G^G[AT]CC
AvrII 520 CCTAGG C^CTAGG
BbsI 668 GTCTTC GAAGAC(2/6)
BcnI 129 CCGGG CC^[GC]GG
BglI 781 GCCTCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 891 AGATCT A^GATCT
BlnI 520 CCTAGG C^CTAGG
BpuAI 668 GTCTTC GAAGAC(2/6)
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BseRI 741 GAGGAG GAGGAG(10/8)
BsiYI 103 CCTGAATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 103 CCTGAATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 488 GTCTC GTCTC(1/5)
BsmFI 635 GGGAC GGGAC(10/14)
BspLU11I 40 ACATGT A^CATGT
BsrFI 85 GCCGGT [GA]^CCGG[CT]
BstEII 124 GGTGACC G^GT[ACGT]ACC
BstPI 124 GGTGACC G^GT[ACGT]ACC
CfoI 601 GCGC GCG^C
Cfr10I 85 GCCGGT [GA]^CCGG[CT]
Cfr13I 806 GGACC G^G[ACGT]CC
DraII 805 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 368 CCATGG C^C[GA][CT]GG
Eam1105I 637 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 600 AGCGCT AGC^GCT
EcoO109I 805 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 124 GGTGACC G^GT[ACGT]ACC
EcoT22I 347 ATGCAT ATGCA^T
HaeII 600 AGCGCT [GA]GCGC^[CT]
HaeIII 780 GGCC GG^CC
HgaI 4 GCGTC GACGC(5/10)
HhaI 601 GCGC GCG^C
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HinP1I 601 GCGC G^CGC
NciI 129 CCGGG CC^[GC]GG
NcoI 368 CCATGG C^CATGG
NsiI 347 ATGCAT ATGCA^T
NspI 40 ACATGT [GA]CATG^[CT]
PleI 35 GAGTC GAGTC(4/5)
PpuMI 805 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 794 CAGCTG CAG^CTG
Sau96I 806 GGACC G^G[ACGT]CC
XcmI 810 CCAAAAAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 300 GTAC GT^AC
682 GTAC
AlwI 187 GATCC GGATC(4/5)
145 GGATC
AlwNI 263 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
648 CAGGTTCTG
Asp700I 345 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
511 GAAGGGGTTC
AvaI 194 CTCGAG C^[CT]CG[GA]G
881 CTCGAG
BanII 744 GAGCCC G[GA]GC[CT]^C
884 GAGCCC
BfaI 521 CTAG C^TAG
604 CTAG
BsmI 345 GAATGC GAATGC(1/-1)
349 GCATTC
BsoBI 194 CTCGAG C^[CT]CG[GA]G
881 CTCGAG
BsrDI 167 GCAATG GCAATG(2/0)
337 GCAATG
BstF5I 52 CATCC GGATG(2/0)
21 GGATG
BstNI 647 CCAGG CC^[AT]GG
736 CCTGG
BstXI 11 CCAGCCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
749 CCATCTCTGTGG
DraI 529 TTTAAA TTT^AAA
583 TTTAAA
EcoRII 647 CCAGG ^CC[AT]GG
736 CCTGG
EcoT14I 368 CCATGG C^C[AT][AT]GG
520 CCTAGG
FokI 52 CATCC GGATG(9/13)
21 GGATG
HapII 86 CCGG C^CGG
129 CCGG
HpaII 86 CCGG C^CGG
129 CCGG
HphI 88 GGTGA GGTGA(8/7)
124 GGTGA
MaeI 521 CTAG C^TAG
604 CTAG
MseI 530 TTAA T^TAA
584 TTAA
MspA1I 761 CAGCGG C[AC]G^C[GT]G
794 CAGCTG
MspI 86 CCGG C^CGG
129 CCGG
MvaI 647 CCAGG CC^[AT]GG
736 CCTGG
PaeR7I 194 CTCGAG C^TCGAG
881 CTCGAG
RsaI 300 GTAC GT^AC
682 GTAC
SmlI 194 CTCGAG C^T[CT][GA]AG
881 CTCGAG
StyI 368 CCATGG C^C[AT][AT]GG
520 CCTAGG
Tru9I 530 TTAA T^TAA
584 TTAA
Tsp45I 37 GTCAC ^GT[GC]AC
125 GTGAC
TspRI 612 CAGTG CA[GC]TG[ACGT][ACGT]^
926 CAGTG
XhoI 194 CTCGAG C^TCGAG
881 CTCGAG
XmnI 345 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
511 GAAGGGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccI AcsI AluI ApoI BbvI
BmyI BsaJI Bsp1286I BsrI BssKI
BstYI DdeI DpnII EarI Fnu4HI
HinfI ItaI Ksp632I MaeIII MboI
MboII MflI MnlI MslI MwoI
NdeII NlaIII NlaIV SapI Sau3AI
ScrFI SfaNI SfcI TaqI TfiI
TseI Tsp509I TthHB8I XhoII