Restriction map for
pFN21AB8232
(Circular) (Six-base) MAPSORT of: pFN21AB8232.seq Check: 4230 from: 1 to: 5653
pFN21AB8232 5653 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2012 2246 2509 2996 1937
Size: 75 234 263 487 4594
Fragments arranged by size:
4594 487 263 234 75
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 3780 4153 1563
Size: 2217 373 3063
Fragments arranged by size:
3063 2217 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2477 3716 5100 5368 1632
Size: 845 1239 1384 268 1917
Fragments arranged by size:
1917 1384 1239 845 268
AfeI AGC'GCT
Cuts at: 2607 2607
Size: 5653
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5456
AflIII A'CryG_T
Cuts at: 1203 2045 4552 5529 1203
Size: 842 2507 977 1327
Fragments arranged by size:
2507 1327 977 842
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 2647 4382 2647
Size: 1735 3918
AleI CACnn'nnGTG
Cuts at: 1166 2930 1166
Size: 1764 3889
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5653
AlwNI CAG_nnn'CTG
Cuts at: 2273 2658 3396 4968 2273
Size: 385 738 1572 2958
Fragments arranged by size:
2958 1572 738 385
ApaLI G'TGCA_C
Cuts at: 3710 4866 3710
Size: 1156 4497
ApoI r'AATT_y
Cuts at: 1493 2288 2559 2918 2962 3188 3340 5449 1493
Size: 795 271 359 44 226 152 2109 1697
Fragments arranged by size:
2109 1697 795 359 271 226 152 44
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2199 2886 2978 1870
Size: 73 256 687 92 4545
Fragments arranged by size:
4545 687 256 92 73
AvrII C'CTAG_G
Cuts at: 2525 2525
Size: 5653
BamHI G'GATC_C
Cuts at: 2983 3350 2983
Size: 367 5286
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2974 4429 618
Size: 325 217 627 1187 1455 1842
Fragments arranged by size:
1842 1455 1187 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2753 2893 2972 1391
Size: 1362 140 79 4072
Fragments arranged by size:
4072 1362 140 79
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2666 928
Size: 971 767 3915
Fragments arranged by size:
3915 971 767
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3844 3878 1903
Size: 34 1907 34 3678
Fragments arranged by size:
3678 1907 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3545 4755 3545
Size: 1210 4443
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5653
BfrBI ATG'CAT
Cuts at: 2354 2354
Size: 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2792 4264 136
Size: 107 122 71 631 605 1120 1472 1525
Fragments arranged by size:
1525 1472 1120 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2896 5648 2896
Size: 2752 2901
BlpI GC'TnA_GC
Cuts at: 3070 3070
Size: 5653
Bme1580I G_kGCm'C
Cuts at: 1790 2267 3714 4870 1790
Size: 477 1447 1156 2573
Fragments arranged by size:
2573 1447 1156 477
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5653
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2646 2924 3384
Size: 416 53 83 399 24 1229 278 460
Cuts at: 3384 4342 442
Size: 958 1753
Fragments arranged by size:
1753 1229 958 460 416 399 278 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4313 1239
Size: 3074 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3778 4643 4941 3778
Size: 865 298 4490
Fragments arranged by size:
4490 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4316 882
Size: 617 2817 2219
Fragments arranged by size:
2817 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3444 493
Size: 711 2240 2702
Fragments arranged by size:
2702 2240 711
BsaBI GATnn'nnATC
Cuts at: 5647 5647
Size: 5653
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3460 3842 4477
Size: 53 83 186 936 476 1451 382 635
Cuts at: 4477 275
Size: 1451
Fragments arranged by size:
1451 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4086 4484 4758 4905 5316 4086
Size: 398 274 147 411 4423
Fragments arranged by size:
4423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5428
Fragments arranged by size:
5428 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2761 1186
Size: 1575 4078
BseYI C'CCAG_C
Cuts at: 4856 5352 4856
Size: 496 5157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5080
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3103 3864 4892 1540
Size: 275 186 1102 761 1028 2301
Fragments arranged by size:
2301 1102 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3714 3799 4870 3714
Size: 85 1071 4497
Fragments arranged by size:
4497 1071 85
BsmI GAATG_Cn'
Cuts at: 2354 2356 3164 3257 2354
Size: 2 808 93 4750
Fragments arranged by size:
4750 808 93 2
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2267 2753 2893 2972 3714 3799 4870
Size: 399 477 486 140 79 742 85 1071
Cuts at: 4870 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 486 477 399 140 85
79
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 3542 5272 3542
Size: 1730 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3008 844
Size: 2164 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3540 1394
Size: 429 1717 3507
Fragments arranged by size:
3507 1717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2179 2349 4148 4322 65
Size: 1183 931 170 1799 174 1396
Fragments arranged by size:
1799 1396 1183 931 174 170
BsrFI r'CCGG_y
Cuts at: 1700 1810 2090 4297 4484 1700
Size: 110 280 2207 187 2869
Fragments arranged by size:
2869 2207 280 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5653
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 3713 4725 3713
Size: 1012 4641
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5653
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 2129 1184
Size: 945 4708
BstXI CCAn_nnnn'nTGG
Cuts at: 2023 2761 2023
Size: 738 4915
BstYI r'GATC_y
Cuts at: 1972 2150 2191 2896 2983 3350 3735 3752 5204
Size: 178 41 705 87 367 385 17 1452
Cuts at: 5204 5290 5302 5648 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 705 385 367 346 178 87
86 41 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2373 513
Size: 552 939 369 3793
Fragments arranged by size:
3793 939 552 369
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4914
Fragments arranged by size:
4914 683 56
BtsI GCAGTG_nn'
Cuts at: 3169 3963 3983 3169
Size: 794 20 4839
Fragments arranged by size:
4839 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5618
DraI TTT'AAA
Cuts at: 2536 2590 2957 2536
Size: 54 367 5232
Fragments arranged by size:
5232 367 54
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4660 809
Size: 507 3344 1802
Fragments arranged by size:
3344 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3100 3989 5618
Size: 54 1425 50 275 18 1270 889 1629
Cuts at: 5618 8
Size: 43
Fragments arranged by size:
1629 1425 1270 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2037 2229 2444 2458 3583 1353
Size: 684 192 215 14 1125 3423
Fragments arranged by size:
3423 1125 684 215 192 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2477 3716 4313 5100 5368 1239
Size: 393 845 1239 597 787 268 1524
Fragments arranged by size:
1524 1239 845 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2811 2811
Size: 5653
EcoRI G'AATT_C
Cuts at: 2962 3340 2962
Size: 378 5275
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 2609 4800 1678
Size: 931 2191 2531
Fragments arranged by size:
2531 2191 931
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2167 2633 2665 2869
Size: 32 135 32 563 32 466 32 204
Cuts at: 2869 2901 4294 4326 4368 4400 1373
Size: 32 1393 32 42 32 2626
Fragments arranged by size:
2626 1393 563 466 204 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2997 3243 669
Size: 988 281 1059 246 3079
Fragments arranged by size:
3079 1059 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2185 2688 4767 4951 1305
Size: 880 503 2079 184 2007
Fragments arranged by size:
2079 2007 880 503 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3831
Fragments arranged by size:
3831 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2061 2372 2759 2930 3611 3970 4129
Size: 648 895 311 387 171 681 359 159
Cuts at: 4129 518
Size: 2042
Fragments arranged by size:
2042 895 681 648 387 359 311 171
159
MspA1I CmG'CkG
Cuts at: 1606 2768 2801 3069 3746 4894 5139 1606
Size: 1162 33 268 677 1148 245 2120
Fragments arranged by size:
2120 1162 1148 677 268 245 33
NcoI C'CATG_G
Cuts at: 513 1065 2004 2373 513
Size: 552 939 369 3793
Fragments arranged by size:
3793 939 552 369
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NsiI A_TGCA'T
Cuts at: 2356 2356
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 2049 3011 4556 1132
Size: 97 820 962 1545 2229
Fragments arranged by size:
2229 1545 962 820 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5653
PciI A'CATG_T
Cuts at: 2045 4552 2045
Size: 2507 3146
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5653
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 5235 5267 3722
Size: 32 1481 32 4108
Fragments arranged by size:
4108 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2811 2811
Size: 5653
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PspXI vC'TCGA_Gb
Cuts at: 2886 2886
Size: 5653
PstI C_TGCA'G
Cuts at: 830 3005 830
Size: 2175 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
PvuII CAG'CTG
Cuts at: 2801 2801
Size: 5653
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SapI GCTCTTCn'nnn_
Cuts at: 2037 2444 2458 2037
Size: 407 14 5232
Fragments arranged by size:
5232 407 14
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 3901 5613 3901
Size: 1712 3941
SfcI C'TryA_G
Cuts at: 826 1045 2083 2478 2506 2869 2907 3001 4136
Size: 219 1038 395 28 363 38 94 1135
Cuts at: 4136 4817 5008 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1038 681 395 363 219 191
94 38 28
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2980 1872
Size: 1108 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2199 2886 3757 4658 4920 820
Size: 197 926 256 687 871 901 262 1553
Fragments arranged by size:
1553 926 901 871 687 262 256 197
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5653
StyI C'CwwG_G
Cuts at: 513 1065 2004 2373 2525 3092 513
Size: 552 939 369 152 567 3074
Fragments arranged by size:
3074 939 567 552 369 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3680 3697 3850 4035 4480 325
Size: 1255 2100 17 153 185 445 1498
Fragments arranged by size:
2100 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2686 3899 5611
Size: 274 80 33 51 1443 709 1213 1712
Cuts at: 5611 96
Size: 138
Fragments arranged by size:
1712 1443 1213 709 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5613 5645 5613
Size: 32 5621
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5653
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2822 2822
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 2199 2886 1943
Size: 256 687 4710
Fragments arranged by size:
4710 687 256
XmaI C'CCGG_G
Cuts at: 1870 2978 1870
Size: 1108 4545
XmnI GAAnn'nnTTC
Cuts at: 2354 2520 3782 2354
Size: 166 1262 4225
Fragments arranged by size:
4225 1262 166
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspOMI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI