RAD1 ORF clone FHC08307

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Halotag ORF Clone FHC08307

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Clone info.

Species

human

Clone Name

pFN21AB6324 Sequence (with vector) Restriction Map

Product ID

FHC08307

Flexi Clone PID

FXC08307

ORF Sequence

DNA sequence (846 bp)
Protein sequence (282 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB528352]

Gene

KIBB6324

Symbol

RAD1

Alias : HRAD1, REC1

Description

RAD1 checkpoint DNA exonuclease, transcript variant 1

Original Clone ID

cs00924

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 846 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 282 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3905.1 7e-127 100.0 RAD1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR003021 19 36 PR01245 Repair protein Rad1/Rec1 IPR003011 41 54 PR01246 Repair protein Rad1 IPR003021 44 61 PR01245 Repair protein Rad1/Rec1 IPR003021 61 77 PR01245 Repair protein Rad1/Rec1 IPR003011 80 96 PR01246 Repair protein Rad1 IPR003021 108 125 PR01245 Repair protein Rad1/Rec1 IPR003021 127 147 PR01245 Repair protein Rad1/Rec1 IPR003011 203 216 PR01246 Repair protein Rad1 IPR003021 223 240 PR01245 Repair protein Rad1/Rec1 IPR003011 223 241 PR01246 Repair protein Rad1 IPR003021 240 257 PR01245 Repair protein Rad1/Rec1 IPR003011 254 267 PR01246 Repair protein Rad1 IPR003021 261 276 PR01245 Repair protein Rad1/Rec1 HMMPfam IPR003021 17 257 PF02144 Repair protein Rad1/Rec1
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002844 282 603153 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB6324 Download

>KIBB6324  846 bp
ATGCCCCTTCTGACCCAACAGATCCAAGACGAGGATGATCAGTACAGCCTTGTGGCCAGC
CTTGACAACGTTAGGAATCTCTCCACTATCTTGAAAGCTATTCATTTCCGAGAACATGCC
ACGTGTTTCGCAACTAAAAATGGTATCAAAGTAACAGTGGAAAATGCAAAGTGTGTGCAA
GCAAATGCTTTTATTCAGGCTGGAATATTTCAGGAGTTTAAAGTTCAGGAAGAGTCTGTT
ACTTTTCGAATTAATTTAACTGTCCTTTTAGACTGTTTATCTATTTTTGGATCAAGTCCT
ATGCCAGGGACTTTAACTGCACTTCGAATGTGTTACCAAGGTTATGGTTACCCTTTGATG
CTGTTCCTGGAAGAAGGAGGAGTGGTGACAGTCTGCAAAATCAATACACAGGAACCTGAG
GAGACCCTGGACTTTGATTTCTGCAGCACCAATGTTATTAATAAAATTATTCTGCAGTCA
GAGGGGCTCCGTGAAGCATTTTCTGAATTGGATATGACGAGTGAAGTCCTACAAATTACC
ATGTCTCCTGACAAGCCTTATTTCAGGTTATCTACTTTTGGAAATGCAGGAAGTTCCCAC
CTTGACTATCCCAAAGATTCTGATTTGATGGAAGCATTTCATTGTAATCAGACCCAAGTC
AACAGATACAAGATTTCCTTACTGAAACCCTCTACAAAGGCATTAGTCCTATCTTGTAAG
GTATCTATTCGGACAGATAACAGAGGCTTCCTTTCATTACAGTATATGATTAGAAATGAA
GATGGACAAATATGTTTTGTGGAATATTACTGCTGCCCTGATGAAGAAGTTCCTGAATCT
GAGTCT

ORF Protein

Cloned ORF protein sequence for pFN21AB6324 Download

>KIBB6324  282 aa
MPLLTQQIQDEDDQYSLVASLDNVRNLSTILKAIHFREHATCFATKNGIKVTVENAKCVQ
ANAFIQAGIFQEFKVQEESVTFRINLTVLLDCLSIFGSSPMPGTLTALRMCYQGYGYPLM
LFLEEGGVVTVCKINTQEPEETLDFDFCSTNVINKIILQSEGLREAFSELDMTSEVLQIT
MSPDKPYFRLSTFGNAGSSHLDYPKDSDLMEAFHCNQTQVNRYKISLLKPSTKALVLSCK
VSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEEVPESES

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB6324 Download

>pFN21AB6324  5551 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCCCTTCTGACCCAACAGATCCAAGACGAGGA
TGATCAGTACAGCCTTGTGGCCAGCCTTGACAACGTTAGGAATCTCTCCACTATCTTGAA
AGCTATTCATTTCCGAGAACATGCCACGTGTTTCGCAACTAAAAATGGTATCAAAGTAAC
AGTGGAAAATGCAAAGTGTGTGCAAGCAAATGCTTTTATTCAGGCTGGAATATTTCAGGA
GTTTAAAGTTCAGGAAGAGTCTGTTACTTTTCGAATTAATTTAACTGTCCTTTTAGACTG
TTTATCTATTTTTGGATCAAGTCCTATGCCAGGGACTTTAACTGCACTTCGAATGTGTTA
CCAAGGTTATGGTTACCCTTTGATGCTGTTCCTGGAAGAAGGAGGAGTGGTGACAGTCTG
CAAAATCAATACACAGGAACCTGAGGAGACCCTGGACTTTGATTTCTGCAGCACCAATGT
TATTAATAAAATTATTCTGCAGTCAGAGGGGCTCCGTGAAGCATTTTCTGAATTGGATAT
GACGAGTGAAGTCCTACAAATTACCATGTCTCCTGACAAGCCTTATTTCAGGTTATCTAC
TTTTGGAAATGCAGGAAGTTCCCACCTTGACTATCCCAAAGATTCTGATTTGATGGAAGC
ATTTCATTGTAATCAGACCCAAGTCAACAGATACAAGATTTCCTTACTGAAACCCTCTAC
AAAGGCATTAGTCCTATCTTGTAAGGTATCTATTCGGACAGATAACAGAGGCTTCCTTTC
ATTACAGTATATGATTAGAAATGAAGATGGACAAATATGTTTTGTGGAATATTACTGCTG
CCCTGATGAAGAAGTTCCTGAATCTGAGTCTGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT