Restriction map for
pFN21AB6324
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AcsI AcyI AflII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI ApoI AscI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstUI BstZ17I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I EcoRI EcoRV EcoT22I FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaI
HpaII KasI KpnI KspI MaeI
MamI MfeI MluI MroI MslI
MspA1I MspI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AclI 67 AACGTT AA^CGTT
AfaI 42 GTAC GT^AC
AflIII 121 ACGTGT A^C[GA][CT]GT
AluI 96 AGCT AG^CT
AocI 415 CCTGAGG CC^T[ACGT]AGG
BalI 53 TGGCCA TGG^CCA
BanII 484 GGGCTC G[GA]GC[CT]^C
BbrPI 120 CACGTG CAC^GTG
BclI 36 TGATCA T^GATCA
BmyI 484 GGGCTC G[AGT]GC[ACT]^C
BsaAI 120 CACGTG [CT]AC^GT[GA]
BsaI 421 GAGACC GGTCTC(1/5)
BsgI 317 CTGCAC GTGCAG(16/14)
BsmFI 307 GGGAC GGGAC(10/14)
Bsp1286I 484 GGGCTC G[AGT]GC[ACT]^C
BstEII 346 GGTTACC G^GT[ACGT]ACC
BstF5I 33 GGATG GGATG(2/0)
BstPI 346 GGTTACC G^GT[ACGT]ACC
BstXI 336 CCAAGGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 20 AGATCC [GA]^GATC[CT]
Bsu36I 415 CCTGAGG CC^T[ACGT]AGG
DraI 217 TTTAAA TTT^AAA
EaeI 53 TGGCCA [CT]^GGCC[GA]
EarI 229 GAAGAG CTCTTC(1/4)
Eco81I 415 CCTGAGG CC^T[ACGT]AGG
EcoNI 298 CCTATGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 346 GGTTACC G^GT[ACGT]ACC
EcoT14I 336 CCAAGG C^C[AT][AT]GG
FbaI 36 TGATCA T^GATCA
FokI 33 GGATG GGATG(9/13)
HaeIII 54 GGCC GG^CC
HincII 658 GTCAAC GT[CT]^[GA]AC
HindII 658 GTCAAC GT[CT]^[GA]AC
HphI 384 GGTGA GGTGA(8/7)
Ksp632I 229 GAAGAG CTCTTC(1/4)
MflI 20 AGATCC [GA]^GATC[CT]
MluNI 53 TGGCCA TGG^CCA
MscI 53 TGGCCA TGG^CCA
NspI 114 ACATGC [GA]CATG^[CT]
PmaCI 120 CACGTG CAC^GTG
PmlI 120 CACGTG CAC^GTG
PshAI 651 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 67 AACGTT AA^CGTT
RsaI 42 GTAC GT^AC
SfaNI 357 GATGC GCATC(5/9)
StyI 336 CCAAGG C^C[AT][AT]GG
Tsp45I 385 GTGAC ^GT[GC]AC
TspRI 155 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 20 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
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AlwI 21 GATCC GGATC(4/5)
289 GGATC
AseI 250 ATTAAT AT^TAAT
457 ATTAAT
AsnI 250 ATTAAT AT^TAAT
457 ATTAAT
Asp700I 631 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
823 GAAGAAGTTC
BbvI 443 GCAGC GCAGC(8/12)
812 GCTGC
BseRI 377 GAGGAG GAGGAG(10/8)
418 GAGGAG
BsiYI 24 CCAAGACGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
298 CCTATGCCAGG
BslI 24 CCAAGACGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
298 CCTATGCCAGG
BsmAI 421 GAGAC GTCTC(1/5)
543 GTCTC
BstBI 245 TTCGAA TT^CGAA
323 TTCGAA
Cac8I 55 GCCAGC GC[ACGT]^[ACGT]GC
177 GCAAGC
DdeI 416 CTGAG C^T[ACGT]AG
839 CTGAG
Fnu4HI 443 GCAGC GC^[ACGT]GC
812 GCTGC
ItaI 443 GCAGC GC^[ACGT]GC
812 GCTGC
MaeII 68 ACGT A^CGT
121 ACGT
NlaIII 115 CATG CATG^
540 CATG
NlaIV 411 GGAACC GG[ACGT]^[ACGT]CC
485 GGCTCC
NspV 245 TTCGAA TT^CGAA
323 TTCGAA
PleI 232 GAGTC GAGTC(4/5)
841 GAGTC
PshBI 250 ATTAAT AT^TAAT
457 ATTAAT
PstI 441 CTGCAG CTGCA^G
472 CTGCAG
SfcI 441 CTGCAG C^T[GA][CT]AG
472 CTGCAG
SfuI 245 TTCGAA TT^CGAA
323 TTCGAA
SspI 204 AATATT AAT^ATT
803 AATATT
TaqI 246 TCGA T^CGA
324 TCGA
TseI 443 GCAGC G^C[AT]GC
812 GCTGC
TthHB8I 246 TCGA T^CGA
324 TCGA
XmnI 631 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
823 GAAGAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
BsaJI BssKI BstNI DpnII EcoRII
HinfI MaeIII MboI MboII MnlI
MseI MvaI NdeII Sau3AI ScrFI
TfiI Tru9I Tsp509I