(Circular) (Six-base) MAPSORT of: pFN21AB6324.seq Check: 6474 from: 1 to: 5551 pFN21AB6324 5551 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3361 278 Size: 53 83 186 936 1825 2468 Fragments arranged by size: 2468 1825 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2894 1937 Size: 957 4594 Acc65I G'GTAC_C Cuts at: 2872 2872 Size: 5551 AclI AA'CG_TT Cuts at: 1563 2073 3678 4051 1563 Size: 510 1605 373 3063 Fragments arranged by size: 3063 1605 510 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3614 4998 5266 1632 Size: 1982 1384 268 1917 Fragments arranged by size: 1982 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5354 AflIII A'CryG_T Cuts at: 1203 2126 4450 5427 1203 Size: 923 2324 977 1327 Fragments arranged by size: 2324 1327 977 923 AgeI A'CCGG_T Cuts at: 4382 4382 Size: 5551 AhdI GACnn_n'nnGTC Cuts at: 4280 4280 Size: 5551 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5551 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2205 1184 Size: 1021 4530 AlwNI CAG_nnn'CTG Cuts at: 3294 4866 3294 Size: 1572 3979 ApaLI G'TGCA_C Cuts at: 3608 4764 3608 Size: 1156 4395 ApoI r'AATT_y Cuts at: 1493 2860 3086 3238 5347 1493 Size: 1367 226 152 2109 1697 Fragments arranged by size: 2109 1697 1367 226 152 AseI AT'TA_AT Cuts at: 160 1032 2256 2463 4105 160 Size: 872 1224 207 1642 1606 Fragments arranged by size: 1642 1606 1224 872 207 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5551 AvaI C'yCGr_G Cuts at: 1870 1943 2876 1870 Size: 73 933 4545 Fragments arranged by size: 4545 933 73 BamHI G'GATC_C Cuts at: 2881 3248 2881 Size: 367 5184 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2872 4327 618 Size: 325 217 627 1085 1455 1842 Fragments arranged by size: 1842 1455 1085 627 325 217 BanII G_rGCy'C Cuts at: 1391 2493 2870 1391 Size: 1102 377 4072 Fragments arranged by size: 4072 1102 377 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4580 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3742 3776 1903 Size: 34 50 34 1721 34 3678 Fragments arranged by size: 3678 1721 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3443 4653 3443 Size: 1210 4341 BclI T'GATC_A Cuts at: 1548 2041 1548 Size: 493 5058 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4162 136 Size: 107 122 71 631 605 2490 1525 Fragments arranged by size: 2490 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5546 5546 Size: 5551 BlpI GC'TnA_GC Cuts at: 2968 2968 Size: 5551 Bme1580I G_kGCm'C Cuts at: 1790 3612 4768 1790 Size: 1822 1156 2573 Fragments arranged by size: 2573 1822 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5551 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3282 4240 442 Size: 416 53 83 399 24 1865 958 1753 Fragments arranged by size: 1865 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5551 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4211 1239 Size: 2972 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3676 4541 4839 3676 Size: 865 298 4388 Fragments arranged by size: 4388 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2420 4214 882 Size: 617 921 1794 2219 Fragments arranged by size: 2219 1794 921 617 BsaAI yAC'GTr Cuts at: 493 1204 2127 3342 493 Size: 711 923 1215 2702 Fragments arranged by size: 2702 1215 923 711 BsaBI GATnn'nnATC Cuts at: 5545 5545 Size: 5551 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3358 3740 4375 275 Size: 53 83 186 936 1825 382 635 1451 Fragments arranged by size: 1825 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3984 4382 4656 4803 5214 3984 Size: 398 274 147 411 4321 Fragments arranged by size: 4321 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2373 2403 2533 2563 1178 Size: 30 165 30 970 30 130 30 4166 Fragments arranged by size: 4166 970 165 130 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2397 2438 1186 Size: 1211 41 4299 Fragments arranged by size: 4299 1211 41 BseYI C'CCAG_C Cuts at: 4754 5250 4754 Size: 496 5055 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2307 1163 Size: 573 571 4407 Fragments arranged by size: 4407 573 571 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3001 3762 4790 1540 Size: 275 186 1000 761 1028 2301 Fragments arranged by size: 2301 1028 1000 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3612 3697 4768 3612 Size: 85 1071 4395 Fragments arranged by size: 4395 1071 85 BsmI GAATG_Cn' Cuts at: 3062 3155 3062 Size: 93 5458 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2493 2870 3612 3697 4768 1391 Size: 399 703 377 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 703 399 377 85 BspEI T'CCGG_A Cuts at: 5214 5214 Size: 5551 BspHI T'CATG_A Cuts at: 3440 5170 3440 Size: 1730 3821 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2906 844 Size: 2062 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3438 1394 Size: 429 1615 3507 Fragments arranged by size: 3507 1615 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4046 4220 65 Size: 1183 2798 174 1396 Fragments arranged by size: 2798 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4195 4382 1700 Size: 110 2385 187 2869 Fragments arranged by size: 2869 2385 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5551 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5551 BssSI C'ACGA_G Cuts at: 3611 4623 3611 Size: 1012 4539 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5551 BstBI TT'CG_AA Cuts at: 2251 2329 4355 2251 Size: 78 2026 3447 Fragments arranged by size: 3447 2026 78 BstEII G'GTnAC_C Cuts at: 1184 2351 1184 Size: 1167 4384 BstXI CCAn_nnnn'nTGG Cuts at: 2348 2348 Size: 5551 BstYI r'GATC_y Cuts at: 1972 2025 2881 3248 3633 3650 5102 5188 5200 Size: 53 856 367 385 17 1452 86 12 Cuts at: 5200 5546 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 856 385 367 346 86 53 17 12 Bsu36I CC'TnA_GG Cuts at: 2421 2421 Size: 5551 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4999 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4812 Fragments arranged by size: 4812 683 56 BtsI GCAGTG_nn' Cuts at: 3067 3861 3881 3067 Size: 794 20 4737 Fragments arranged by size: 4737 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5551 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5516 DraI TTT'AAA Cuts at: 2224 2855 2224 Size: 631 4920 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4558 809 Size: 507 3242 1802 Fragments arranged by size: 3242 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2998 3887 Size: 54 1425 50 275 18 228 940 889 Cuts at: 3887 5516 8 Size: 1629 43 Fragments arranged by size: 1629 1425 940 889 275 228 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2998 1537 Size: 275 1186 4090 Fragments arranged by size: 4090 1186 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2229 3481 1353 Size: 876 1252 3423 Fragments arranged by size: 3423 1252 876 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4138 4512 4658 387 Size: 1064 138 2549 374 146 1280 Fragments arranged by size: 2549 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3614 4211 4998 5266 1239 Size: 393 1982 597 787 268 1524 Fragments arranged by size: 1982 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2307 2307 Size: 5551 EcoRI G'AATT_C Cuts at: 2860 3238 2860 Size: 378 5173 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2698 2698 Size: 5551 FspI TGC'GCA Cuts at: 4057 4057 Size: 5551 HaeII r_GCGC'y Cuts at: 1678 4698 1678 Size: 3020 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2267 2299 4192 4224 4266 Size: 32 135 32 695 32 1893 32 42 Cuts at: 4266 4298 1373 Size: 32 2626 Fragments arranged by size: 2626 1893 695 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2665 2895 3141 669 Size: 988 281 727 230 246 3079 Fragments arranged by size: 3079 988 727 281 246 230 HindIII A'AGCT_T Cuts at: 748 748 Size: 5551 HpaI GTT'AAC Cuts at: 3141 3141 Size: 5551 KpnI G_GTAC'C Cuts at: 2876 2876 Size: 5551 MfeI C'AATT_G Cuts at: 3150 3150 Size: 5551 MluI A'CGCG_T Cuts at: 5427 5427 Size: 5551 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4665 4849 1305 Size: 3360 184 2007 Fragments arranged by size: 3360 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2060 10 Size: 54 1425 343 228 3501 Fragments arranged by size: 3501 1425 343 228 54 MslI CAynn'nnrTG Cuts at: 518 1166 3509 3868 4027 518 Size: 648 2343 359 159 2042 Fragments arranged by size: 2343 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2967 3644 4792 5037 1606 Size: 1361 677 1148 245 2120 Fragments arranged by size: 2120 1361 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4999 NdeI CA'TA_TG Cuts at: 387 387 Size: 5551 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5551 NotI GC'GGCC_GC Cuts at: 2998 2998 Size: 5551 NspI r_CATG'y Cuts at: 1132 1229 2123 2909 4454 1132 Size: 97 894 786 1545 2229 Fragments arranged by size: 2229 1545 894 786 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5551 PciI A'CATG_T Cuts at: 4450 4450 Size: 5551 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5551 PfoI T'CCnGG_A Cuts at: 2370 2370 Size: 5551 PmeI GTTT'AAAC Cuts at: 2855 2855 Size: 5551 PmlI CAC'GTG Cuts at: 2127 2127 Size: 5551 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3620 3652 5133 5165 3620 Size: 32 1481 32 4006 Fragments arranged by size: 4006 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2660 1613 Size: 1047 4504 PsiI TTA'TAA Cuts at: 3121 3121 Size: 5551 PstI C_TGCA'G Cuts at: 830 2450 2481 2903 830 Size: 1620 31 422 3478 Fragments arranged by size: 3478 1620 422 31 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5551 SalI G'TCGA_C Cuts at: 1936 2893 1936 Size: 957 4594 SbfI CC_TGCA'GG Cuts at: 2903 2903 Size: 5551 ScaI AGT'ACT Cuts at: 3799 5511 3799 Size: 1712 3839 SfcI C'TryA_G Cuts at: 826 1045 2446 2477 2899 4034 4715 4906 826 Size: 219 1401 31 422 1135 681 191 1471 Fragments arranged by size: 1471 1401 1135 681 422 219 191 31 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5551 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5551 SmaI CCC'GGG Cuts at: 1872 2878 1872 Size: 1006 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3655 4556 4818 820 Size: 197 926 1712 901 262 1553 Fragments arranged by size: 1712 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3342 493 Size: 2849 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5551 SphI G_CATG'C Cuts at: 1132 2909 1132 Size: 1777 3774 SspI AAT'ATT Cuts at: 5 52 2211 2810 3475 5 Size: 47 2159 599 665 2081 Fragments arranged by size: 2159 2081 665 599 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5551 StyI C'CwwG_G Cuts at: 513 1065 2341 2990 513 Size: 552 1276 649 3074 Fragments arranged by size: 3074 1276 649 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3578 3595 3748 3933 4378 325 Size: 1255 1998 17 153 185 445 1498 Fragments arranged by size: 1998 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2046 3797 5509 Size: 274 80 33 51 1443 69 1751 1712 Cuts at: 5509 96 Size: 138 Fragments arranged by size: 1751 1712 1443 274 138 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2373 2405 5511 5543 2373 Size: 32 3106 32 2381 Fragments arranged by size: 3106 2381 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5551 XbaI T'CTAG_A Cuts at: 2887 2887 Size: 5551 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5551 XmaI C'CCGG_G Cuts at: 1870 2876 1870 Size: 1006 4545 XmnI GAAnn'nnTTC Cuts at: 2640 2832 3680 2640 Size: 192 848 4511 Fragments arranged by size: 4511 848 192 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3359 276 Size: 53 83 186 936 1825 2468 Fragments arranged by size: 2468 1825 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI