APPL1 ORF clone FHC04145

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC04145

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Clone info.

Species

human

Clone Name

pFN21AA1428 Sequence (with vector) Restriction Map

Product ID

FHC04145

Flexi Clone PID

FXC04145

ORF Sequence

DNA sequence (2127 bp)
Protein sequence (709 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB587353]

Gene

KIAA1428

Symbol

APPL1

Alias : APPL, DIP13alpha, MODY14

Description

adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1

Original Clone ID

fh02979s1

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2127 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 709 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2882.1 0 100.0 APPL1 CCDS9101.1 6.5e-104 55.0 APPL2 CCDS58276.1 4.2e-103 54.6 APPL2 CCDS58275.1 3.4e-94 54.1 APPL2 CCDS19.2 3.6e-15 24.2 ACAP3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR006020 502 629 PF00640 Phosphotyrosine interaction domain HMMSmart IPR001849 278 377 SM00233 Pleckstrin homology domain IPR006020 497 636 SM00462 Phosphotyrosine interaction domain ProfileScan IPR001849 277 375 PS50003 Pleckstrin homology domain IPR006020 502 638 PS01179 Phosphotyrosine interaction domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_036228 709 604299,616511 100.0 100.0 XP_011531885 692 604299,616511 100.0 97.5 NP_060641 664 606231 55.0 88.2 NP_001238833 670 606231 54.6 88.2 XP_016875040 651 606231 54.7 86.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA1428 Download

>KIAA1428  2127 bp
ATGCCGGGGATCGACAAGCTGCCCATCGAGGAGACGCTGGAGGACAGCCCGCAGACAAGG
TCTTTACTAGGTGTATTTGAAGAAGATGCCACAGCTATTTCCAACTATATGAACCAGTTG
TATCAAGCTATGCATCGGATTTATGATGCACAGAATGAATTAAGTGCAGCAACACACCTG
ACCTCAAAACTTTTAAAAGAATATGAAAAACAGCGTTTTCCATTGGGAGGTGATGATGAA
GTTATGAGCTCTACATTGCAACAGTTTTCAAAAGTTATAGATGAGCTTAGCTCTTGTCAT
GCAGTGCTTTCAACTCAACTTGCTGATGCCATGATGTTCCCCATTACCCAGTTTAAAGAA
AGAGATCTGAAAGAAATACTAACATTAAAGGAAGTATTTCAGATTGCAAGTAATGATCAT
GATGCCGCGATTAATAGATATAGCCGTTTATCAAAAAAAAGAGAAAATGACAAGGTGAAG
TATGAAGTAACAGAAGATGTGTACACATCCAGAAAGAAACAACACCAGACCATGATGCAT
TATTTTTGTGCATTAAATACTCTTCAGTACAAGAAGAAAATAGCATTGTTAGAACCTCTA
CTTGGGTACATGCAAGCTCAGATAAGTTTCTTTAAGATGGGTTCTGAAAATCTTAATGAA
CAACTGGAAGAATTTTTAGCTAATATTGGAACAAGCGTTCAGAATGTTCGCAGGGAAATG
GACAGTGATATAGAGACCATGCAACAGACAATAGAGGATTTGGAAGTAGCCAGTGATCCC
TTATATGTGCCTGACCCAGACCCCACCAAATTTCCTGTTAATCGAAATTTAACCCGAAAG
GCTGGATACCTTAATGCTAGGAATAAAACAGGCTTGGTGTCATCTACCTGGGACAGACAG
TTTTACTTCACGCAGGGTGGAAATTTAATGAGTCAGGCCCGTGGGGATGTAGCAGGAGGC
CTGGCCATGGACATAGACAACTGTTCAGTGATGGCTGTGGACTGTGAAGACAGACGATAT
TGTTTTCAGATCACCTCTTTCGATGGAAAAAAATCTTCAATTTTGCAAGCAGAGAGTAAA
AAAGATCATGAAGAGTGGATCTGTACAATAAACAACATATCTAAACAAATATACTTAAGT
GAAAATCCAGAGGAAACTGCTGCACGAGTAAATCAATCAGCTCTGGAAGCTGTCACTCCT
TCCCCATCTTTCCAGCAGAGGCACGAGAGCCTGCGGCCAGCAGCAGGACAATCTCGGCCA
CCGACAGCTCGAACCAGCAGTTCAGGATCCTTAGGATCTGAGTCTACAAATTTGGCTGCC
CTCTCTCTAGATTCTCTTGTTGCCCCAGACACCCCAATACAGTTTGACATAATTTCTCCT
GTGTGTGAAGATCAGCCTGGCCAGGCAAAAGCCTTTGGCCAGGGAGGCAGGCGTACAAAT
CCATTTGGAGAATCTGGAGGAAGTACAAAATCTGAAACTGAAGATTCTATTCTTCATCAG
TTATTTATTGTCCGATTCCTTGGTTCAATGGAGGTGAAATCAGATGACCATCCAGATGTT
GTTTATGAAACTATGCGCCAAATCTTAGCTGCCCGGGCCATCCATAACATCTTTCGTATG
ACAGAATCGCATTTATTAGTCACTTGTGACTGTTTAAAGTTAATTGATCCACAGACACAA
GTTACAAGGCTCACGTTTCCATTACCTTGTGTAGTTTTGTATGCTACACACCAGGAAAAT
AAGCGCCTTTTTGGATTTGTTCTTCGGACATCAAGCGGGAGAAGTGAAAGTAATCTGTCA
TCAGTCTGCTATATATTTGAGTCAAACAATGAGGGGGAAAAGATATGTGATTCTGTTGGA
CTGGCAAAACAGATAGCTTTGCATGCTGAACTGGATCGTAGGGCATCAGAAAAACAAAAA
GAAATAGAGAGAGTAAAAGAGAAGCAACAGAAAGAACTCAATAAACAAAAACAGATTGAA
AAGGACTTGGAAGAACAAAGTCGGTTGATAGCTGCTTCCAGTAGACCAAACCAAGCCAGT
AGTGAGGGGCAGTTTGTTGTCCTTAGCAGTAGCCAGTCAGAAGAGAGTGATTTGGGAGAA
GGAGGAAAGAAGAGAGAATCAGAAGCA

ORF Protein

Cloned ORF protein sequence for pFN21AA1428 Download

>KIAA1428  709 aa
MPGIDKLPIEETLEDSPQTRSLLGVFEEDATAISNYMNQLYQAMHRIYDAQNELSAATHL
TSKLLKEYEKQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPITQFKE
RDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMH
YFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNEQLEEFLANIGTSVQNVRREM
DSDIETMQQTIEDLEVASDPLYVPDPDPTKFPVNRNLTRKAGYLNARNKTGLVSSTWDRQ
FYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESK
KDHEEWICTINNISKQIYLSENPEETAARVNQSALEAVTPSPSFQQRHESLRPAAGQSRP
PTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTN
PFGESGGSTKSETEDSILHQLFIVRFLGSMEVKSDDHPDVVYETMRQILAARAIHNIFRM
TESHLLVTCDCLKLIDPQTQVTRLTFPLPCVVLYATHQENKRLFGFVLRTSSGRSESNLS
SVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEIERVKEKQQKELNKQKQIE
KDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEGGKKRESEA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA1428 Download

>pFN21AA1428  6832 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCGGGGATCGACAAGCTGCCCATCGAGGAGAC
GCTGGAGGACAGCCCGCAGACAAGGTCTTTACTAGGTGTATTTGAAGAAGATGCCACAGC
TATTTCCAACTATATGAACCAGTTGTATCAAGCTATGCATCGGATTTATGATGCACAGAA
TGAATTAAGTGCAGCAACACACCTGACCTCAAAACTTTTAAAAGAATATGAAAAACAGCG
TTTTCCATTGGGAGGTGATGATGAAGTTATGAGCTCTACATTGCAACAGTTTTCAAAAGT
TATAGATGAGCTTAGCTCTTGTCATGCAGTGCTTTCAACTCAACTTGCTGATGCCATGAT
GTTCCCCATTACCCAGTTTAAAGAAAGAGATCTGAAAGAAATACTAACATTAAAGGAAGT
ATTTCAGATTGCAAGTAATGATCATGATGCCGCGATTAATAGATATAGCCGTTTATCAAA
AAAAAGAGAAAATGACAAGGTGAAGTATGAAGTAACAGAAGATGTGTACACATCCAGAAA
GAAACAACACCAGACCATGATGCATTATTTTTGTGCATTAAATACTCTTCAGTACAAGAA
GAAAATAGCATTGTTAGAACCTCTACTTGGGTACATGCAAGCTCAGATAAGTTTCTTTAA
GATGGGTTCTGAAAATCTTAATGAACAACTGGAAGAATTTTTAGCTAATATTGGAACAAG
CGTTCAGAATGTTCGCAGGGAAATGGACAGTGATATAGAGACCATGCAACAGACAATAGA
GGATTTGGAAGTAGCCAGTGATCCCTTATATGTGCCTGACCCAGACCCCACCAAATTTCC
TGTTAATCGAAATTTAACCCGAAAGGCTGGATACCTTAATGCTAGGAATAAAACAGGCTT
GGTGTCATCTACCTGGGACAGACAGTTTTACTTCACGCAGGGTGGAAATTTAATGAGTCA
GGCCCGTGGGGATGTAGCAGGAGGCCTGGCCATGGACATAGACAACTGTTCAGTGATGGC
TGTGGACTGTGAAGACAGACGATATTGTTTTCAGATCACCTCTTTCGATGGAAAAAAATC
TTCAATTTTGCAAGCAGAGAGTAAAAAAGATCATGAAGAGTGGATCTGTACAATAAACAA
CATATCTAAACAAATATACTTAAGTGAAAATCCAGAGGAAACTGCTGCACGAGTAAATCA
ATCAGCTCTGGAAGCTGTCACTCCTTCCCCATCTTTCCAGCAGAGGCACGAGAGCCTGCG
GCCAGCAGCAGGACAATCTCGGCCACCGACAGCTCGAACCAGCAGTTCAGGATCCTTAGG
ATCTGAGTCTACAAATTTGGCTGCCCTCTCTCTAGATTCTCTTGTTGCCCCAGACACCCC
AATACAGTTTGACATAATTTCTCCTGTGTGTGAAGATCAGCCTGGCCAGGCAAAAGCCTT
TGGCCAGGGAGGCAGGCGTACAAATCCATTTGGAGAATCTGGAGGAAGTACAAAATCTGA
AACTGAAGATTCTATTCTTCATCAGTTATTTATTGTCCGATTCCTTGGTTCAATGGAGGT
GAAATCAGATGACCATCCAGATGTTGTTTATGAAACTATGCGCCAAATCTTAGCTGCCCG
GGCCATCCATAACATCTTTCGTATGACAGAATCGCATTTATTAGTCACTTGTGACTGTTT
AAAGTTAATTGATCCACAGACACAAGTTACAAGGCTCACGTTTCCATTACCTTGTGTAGT
TTTGTATGCTACACACCAGGAAAATAAGCGCCTTTTTGGATTTGTTCTTCGGACATCAAG
CGGGAGAAGTGAAAGTAATCTGTCATCAGTCTGCTATATATTTGAGTCAAACAATGAGGG
GGAAAAGATATGTGATTCTGTTGGACTGGCAAAACAGATAGCTTTGCATGCTGAACTGGA
TCGTAGGGCATCAGAAAAACAAAAAGAAATAGAGAGAGTAAAAGAGAAGCAACAGAAAGA
ACTCAATAAACAAAAACAGATTGAAAAGGACTTGGAAGAACAAAGTCGGTTGATAGCTGC
TTCCAGTAGACCAAACCAAGCCAGTAGTGAGGGGCAGTTTGTTGTCCTTAGCAGTAGCCA
GTCAGAAGAGAGTGATTTGGGAGAAGGAGGAAAGAAGAGAGAATCAGAAGCAGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT