(Circular) (Six-base) MAPSORT of: pFN21AA1428.seq Check: 9213 from: 1 to: 6832 pFN21AA1428 6832 bp With 182 enzymes: SgfI * June 30, 2009 15:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4642 278 Size: 53 83 186 936 3106 2468 Fragments arranged by size: 3106 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3308 4027 4175 1937 Size: 1371 719 148 4594 Fragments arranged by size: 4594 1371 719 148 Acc65I G'GTAC_C Cuts at: 4153 4153 Size: 6832 AclI AA'CG_TT Cuts at: 1563 4959 5332 1563 Size: 3396 373 3063 Fragments arranged by size: 3396 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2552 3504 4895 6279 6547 1632 Size: 920 952 1391 1384 268 1917 Fragments arranged by size: 1917 1391 1384 952 920 268 AflII C'TTAA_G Cuts at: 820 1017 3139 820 Size: 197 2122 4513 Fragments arranged by size: 4513 2122 197 AflIII A'CryG_T Cuts at: 1203 5731 6708 1203 Size: 4528 977 1327 Fragments arranged by size: 4528 1327 977 AgeI A'CCGG_T Cuts at: 5663 5663 Size: 6832 AhdI GACnn_n'nnGTC Cuts at: 5561 5561 Size: 6832 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6832 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6832 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2685 2717 2685 Size: 32 6800 AlwNI CAG_nnn'CTG Cuts at: 4575 6147 4575 Size: 1572 5260 ApaLI G'TGCA_C Cuts at: 4889 6045 4889 Size: 1156 5676 ApoI r'AATT_y Cuts at: 1493 2675 2813 2830 2926 3313 4141 4367 4519 Size: 1182 138 17 96 387 828 226 152 Cuts at: 4519 6628 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1182 828 387 226 152 138 96 17 AseI AT'TA_AT Cuts at: 160 1032 2436 5386 160 Size: 872 1404 2950 1606 Fragments arranged by size: 2950 1606 1404 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6832 AvaI C'yCGr_G Cuts at: 1870 1943 3597 4157 1870 Size: 73 1654 560 4545 Fragments arranged by size: 4545 1654 560 73 BamHI G'GATC_C Cuts at: 3290 4162 4529 3290 Size: 872 367 5593 Fragments arranged by size: 5593 872 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4153 5608 618 Size: 325 217 627 2366 1455 1842 Fragments arranged by size: 2366 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2255 4151 1391 Size: 864 1896 4072 Fragments arranged by size: 4072 1896 864 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3018 928 Size: 971 1119 4742 Fragments arranged by size: 4742 1119 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5023 5057 1903 Size: 34 50 34 3002 34 3678 Fragments arranged by size: 3678 3002 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2843 4724 5934 2843 Size: 1881 1210 3741 Fragments arranged by size: 3741 1881 1210 BclI T'GATC_A Cuts at: 1548 2419 1548 Size: 871 5961 BfrBI ATG'CAT Cuts at: 2137 2542 2137 Size: 405 6427 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3429 5443 136 Size: 107 122 71 631 605 1757 2014 1525 Fragments arranged by size: 2014 1757 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2368 6827 2368 Size: 4459 2373 BlpI GC'TnA_GC Cuts at: 2291 4249 2291 Size: 1958 4874 Bme1580I G_kGCm'C Cuts at: 1790 4893 6049 1790 Size: 3103 1156 2573 Fragments arranged by size: 3103 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6832 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2347 4563 5521 Size: 416 53 83 399 24 930 2216 958 Cuts at: 5521 442 Size: 1753 Fragments arranged by size: 2216 1753 958 930 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6832 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2311 2311 Size: 6832 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2063 3480 5492 1239 Size: 824 1417 2012 2579 Fragments arranged by size: 2579 2012 1417 824 Bpu10I CC'TnA_GC Cuts at: 4067 4067 Size: 6832 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4957 5822 6120 4957 Size: 865 298 5669 Fragments arranged by size: 5669 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2732 5495 882 Size: 617 1233 2763 2219 Fragments arranged by size: 2763 2219 1233 617 BsaAI yAC'GTr Cuts at: 493 1204 4623 493 Size: 711 3419 2702 Fragments arranged by size: 3419 2702 711 BsaBI GATnn'nnATC Cuts at: 3552 6826 3552 Size: 3274 3558 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4639 5021 5656 275 Size: 53 83 186 936 3106 382 635 1451 Fragments arranged by size: 3106 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5265 5663 5937 6084 6495 5265 Size: 398 274 147 411 5602 Fragments arranged by size: 5602 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6607 Fragments arranged by size: 6607 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 1186 Size: 862 5970 BseYI C'CCAG_C Cuts at: 6035 6531 6035 Size: 496 6336 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2190 3150 1163 Size: 573 454 960 4845 Fragments arranged by size: 4845 960 573 454 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4282 5043 6071 1540 Size: 275 186 2281 761 1028 2301 Fragments arranged by size: 2301 2281 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2255 4893 4978 6049 2255 Size: 2638 85 1071 3038 Fragments arranged by size: 3038 2638 1071 85 BsmI GAATG_Cn' Cuts at: 4343 4436 4343 Size: 93 6739 BsmBI CGTCTCn'nnnn_ Cuts at: 2030 2030 Size: 6832 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2255 4151 4893 4978 6049 1391 Size: 399 465 1896 742 85 1071 2174 Fragments arranged by size: 2174 1896 1071 742 465 399 85 BspEI T'CCGG_A Cuts at: 6495 6495 Size: 6832 BspHI T'CATG_A Cuts at: 2422 3091 4721 6451 2422 Size: 669 1630 1730 2803 Fragments arranged by size: 2803 1730 1630 669 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4187 844 Size: 3343 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4719 1394 Size: 429 2896 3507 Fragments arranged by size: 3507 2896 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2258 5327 5501 65 Size: 1183 1010 3069 174 1396 Fragments arranged by size: 3069 1396 1183 1010 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5476 5663 1700 Size: 110 3666 187 2869 Fragments arranged by size: 3666 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2505 3107 96 Size: 2409 602 3821 Fragments arranged by size: 3821 2409 602 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6832 BssSI C'ACGA_G Cuts at: 3168 3227 4892 5904 3168 Size: 59 1665 1012 4096 Fragments arranged by size: 4096 1665 1012 59 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6832 BstBI TT'CG_AA Cuts at: 5636 5636 Size: 6832 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6832 BstYI r'GATC_y Cuts at: 1972 2368 3102 3290 3299 4162 4529 4914 4931 Size: 396 734 188 9 863 367 385 17 Cuts at: 4931 6383 6469 6481 6827 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 863 734 396 385 367 346 188 86 17 12 9 Bsu36I CC'TnA_GG Cuts at: 3295 3295 Size: 6832 BtgI C'CryG_G Cuts at: 513 1065 2944 2970 513 Size: 552 1879 26 4375 Fragments arranged by size: 4375 1879 552 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6093 Fragments arranged by size: 6093 683 56 BtsI GCAGTG_nn' Cuts at: 2313 4348 5142 5162 2313 Size: 2035 794 20 3983 Fragments arranged by size: 3983 2035 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6832 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6797 DraI TTT'AAA Cuts at: 2199 2359 3660 4136 2199 Size: 160 1301 476 4895 Fragments arranged by size: 4895 1301 476 160 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5839 809 Size: 507 4523 1802 Fragments arranged by size: 4523 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2967 3239 3260 Size: 54 1425 50 275 18 1137 272 21 Cuts at: 3260 3403 3421 4279 5168 6797 8 Size: 143 18 858 889 1629 43 Fragments arranged by size: 1629 1425 1137 889 858 275 272 143 54 50 43 21 18 18 EagI C'GGCC_G Cuts at: 1537 1812 4279 1537 Size: 275 2467 4090 Fragments arranged by size: 4090 2467 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2571 3090 4080 4109 4762 1353 Size: 1218 519 990 29 653 3423 Fragments arranged by size: 3423 1218 990 653 519 29 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5419 5793 5939 387 Size: 1064 138 3830 374 146 1280 Fragments arranged by size: 3830 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2253 2253 Size: 6832 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2552 3480 3504 4895 5492 6279 Size: 393 431 489 928 24 1391 597 787 Cuts at: 6279 6547 1239 Size: 268 1524 Fragments arranged by size: 1524 1391 928 787 597 489 431 393 268 24 EcoRI G'AATT_C Cuts at: 4141 4519 4141 Size: 378 6454 FspI TGC'GCA Cuts at: 5338 5338 Size: 6832 HaeII r_GCGC'y Cuts at: 1678 3751 5979 1678 Size: 2073 2228 2531 Fragments arranged by size: 2531 2228 2073 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3527 3559 5473 5505 5547 Size: 32 135 32 1955 32 1914 32 42 Cuts at: 5547 5579 1373 Size: 32 2626 Fragments arranged by size: 2626 1955 1914 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4176 4422 669 Size: 988 281 2238 246 3079 Fragments arranged by size: 3079 2238 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6832 HpaI GTT'AAC Cuts at: 4422 4422 Size: 6832 KpnI G_GTAC'C Cuts at: 4157 4157 Size: 6832 MfeI C'AATT_G Cuts at: 4431 4431 Size: 6832 MluI A'CGCG_T Cuts at: 6708 6708 Size: 6832 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2130 3841 5946 6130 1305 Size: 825 1711 2105 184 2007 Fragments arranged by size: 2105 2007 1711 825 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2969 3405 3423 10 Size: 54 1425 343 1137 436 18 3419 Fragments arranged by size: 3419 1425 1137 436 343 54 18 MslI CAynn'nnrTG Cuts at: 518 1166 3558 4790 5149 5308 518 Size: 648 2392 1232 359 159 2042 Fragments arranged by size: 2392 2042 1232 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4248 4925 6073 6318 1606 Size: 2642 677 1148 245 2120 Fragments arranged by size: 2642 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2970 513 Size: 552 1905 4375 Fragments arranged by size: 4375 1905 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6832 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6832 NotI GC'GGCC_GC Cuts at: 4279 4279 Size: 6832 NsiI A_TGCA'T Cuts at: 2139 2544 2139 Size: 405 6427 NspI r_CATG'y Cuts at: 1132 1229 2617 3890 4190 5735 1132 Size: 97 1388 1273 300 1545 2229 Fragments arranged by size: 2229 1545 1388 1273 300 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6832 PciI A'CATG_T Cuts at: 5731 5731 Size: 6832 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6832 PmeI GTTT'AAAC Cuts at: 4136 4136 Size: 6832 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4901 4933 6414 6446 4901 Size: 32 1481 32 5287 Fragments arranged by size: 5287 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6832 PsiI TTA'TAA Cuts at: 4402 4402 Size: 6832 PstI C_TGCA'G Cuts at: 830 4184 830 Size: 3354 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6832 SacI G_AGCT'C Cuts at: 2255 2255 Size: 6832 SalI G'TCGA_C Cuts at: 1936 4174 1936 Size: 2238 4594 SbfI CC_TGCA'GG Cuts at: 4184 4184 Size: 6832 ScaI AGT'ACT Cuts at: 5080 6792 5080 Size: 1712 5120 SfcI C'TryA_G Cuts at: 826 1045 4180 5315 5996 6187 826 Size: 219 3135 1135 681 191 1471 Fragments arranged by size: 3135 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6832 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6832 SmaI CCC'GGG Cuts at: 1872 3599 4159 1872 Size: 1727 560 4545 Fragments arranged by size: 4545 1727 560 SmlI C'TyrA_G Cuts at: 820 1017 1943 3139 4936 5837 6099 820 Size: 197 926 1196 1797 901 262 1553 Fragments arranged by size: 1797 1553 1196 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4623 493 Size: 4130 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6832 SphI G_CATG'C Cuts at: 1132 3890 4190 1132 Size: 2758 300 3774 Fragments arranged by size: 3774 2758 300 SrfI GCCC'GGGC Cuts at: 3599 3599 Size: 6832 SspI AAT'ATT Cuts at: 5 52 2689 4756 5 Size: 47 2637 2067 2081 Fragments arranged by size: 2637 2081 2067 47 StuI AGG'CCT Cuts at: 1517 2964 1517 Size: 1447 5385 StyI C'CwwG_G Cuts at: 513 1065 2970 3523 4271 513 Size: 552 1905 553 748 3074 Fragments arranged by size: 3074 1905 748 553 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4859 4876 5029 5214 5659 325 Size: 1255 3279 17 153 185 445 1498 Fragments arranged by size: 3279 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2505 2571 3107 Size: 274 80 33 51 1443 528 66 536 Cuts at: 3107 3467 5078 6790 96 Size: 360 1611 1712 138 Fragments arranged by size: 1712 1611 1443 536 528 360 274 138 80 66 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2800 2832 6792 6824 2800 Size: 32 3960 32 2808 Fragments arranged by size: 3960 2808 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2062 1316 Size: 746 6086 XbaI T'CTAG_A Cuts at: 3331 4168 3331 Size: 837 5995 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6832 XmaI C'CCGG_G Cuts at: 1870 3597 4157 1870 Size: 1727 560 4545 Fragments arranged by size: 4545 1727 560 XmnI GAAnn'nnTTC Cuts at: 2400 4961 2400 Size: 2561 4271 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4640 276 Size: 53 83 186 936 3106 2468 Fragments arranged by size: 3106 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SwaI XcmI