Restriction map for
pFN21AA1428
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AvaII AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BlnI
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Cfr10I ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MroI
MspA1I MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI Van91I
XcmI XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 426 CGCG CG^CG
AflII 1134 CTTAAG C^TTAAG
AocI 1289 CCTTAGG CC^T[ACGT]AGG
AseI 430 ATTAAT AT^TAAT
AsnI 430 ATTAAT AT^TAAT
Asp700I 391 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 246 GAGCTC G[AT]GC[AT]^C
AspI 54 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1592 CCCGGG C^[CT]CG[GA]G
BamHI 1285 GGATCC G^GATCC
BanII 246 GAGCTC G[GA]GC[CT]^C
BbsI 1006 GAAGAC GAAGAC(2/6)
BciVI 844 GGATAC GTATCC(6/5)
BclI 414 TGATCA T^GATCA
BfrI 1134 CTTAAG C^TTAAG
BglI 1418 GCCAGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 363 AGATCT A^GATCT
BlpI 285 GCTTAGC GC^T[ACGT]AGC
BmyI 246 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 285 GCTTAGC GC^T[ACGT]AGC
BpuAI 1006 GAAGAC GAAGAC(2/6)
BsaBI 1543 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 733 GAGACC GGTCTC(1/5)
BseRI 28 GAGGAG GAGGAG(10/8)
BsiHKAI 246 GAGCTC G[AT]GC[AT]^C
BsmBI 31 GAGACG CGTCTC(1/5)
BsmFI 890 GGGAC GGGAC(10/14)
BsoBI 1592 CCCGGG C^[CT]CG[GA]G
Bsp1286I 246 GAGCTC G[AGT]GC[ACT]^C
BsrDI 254 CATTGC GCAATG(2/0)
BstUI 426 CGCG CG^CG
Bsu36I 1289 CCTTAGG CC^T[ACGT]AGG
CelII 285 GCTTAGC GC^T[ACGT]AGC
Eco81I 1289 CCTTAGG CC^T[ACGT]AGG
FbaI 414 TGATCA T^GATCA
HaeII 1742 AGCGCC [GA]GCGC^[CT]
HgaI 33 GACGC GACGC(5/10)
MaeII 1693 ACGT A^CGT
MamI 1543 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1549 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 426 CGCG CG^CG
NcoI 965 CCATGG C^CATGG
NlaIV 1285 GGATCC GG[ACGT]^[ACGT]CC
PflFI 54 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 430 ATTAAT AT^TAAT
SacI 246 GAGCTC GAGCT^C
SmaI 1592 CCCGGG CCC^GGG
SmlI 1134 CTTAAG C^T[CT][GA]AG
SphI 1881 GCATGC GCATG^C
SspI 682 AATATT AAT^ATT
StuI 957 AGGCCT AGG^CCT
Tth111I 54 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1326 TCTAGA T^CTAGA
XmaCI 1592 CCCGGG C^CCGGG
XmaI 1592 CCCGGG C^CCGGG
XmnI 391 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1302 GTCTAC GT^[AC][GT]AC
2021 GTAGAC
BpmI 37 CTGGAG CTGGAG(16/14)
1454 CTGGAG
BsgI 1160 CTGCAC GTGCAG(16/14)
164 GTGCAG
BsmAI 31 GAGAC GTCTC(1/5)
733 GAGAC
Bsp1407I 500 TGTACA T^GTACA
1102 TGTACA
BspHI 417 TCATGA T^CATGA
1086 TCATGA
BsrGI 500 TGTACA T^GTACA
1102 TGTACA
BssSI 1163 CACGAG CACGAG(-5/-1)
1222 CACGAG
CfoI 1575 GCGC GCG^C
1743 GCGC
Cfr13I 936 GGCCC G^G[ACGT]CC
1595 GGGCC
DsaI 939 CCGTGG C^C[GA][CT]GG
965 CCATGG
EcoT14I 965 CCATGG C^C[AT][AT]GG
1518 CCTTGG
EcoT22I 130 ATGCAT ATGCA^T
535 ATGCAT
FauI 48 CCCGC CCCGC(4/6)
1775 GCGGG
HapII 4 CCGG C^CGG
1593 CCGG
HhaI 1575 GCGC GCG^C
1743 GCGC
HinP1I 1575 GCGC G^CGC
1743 GCGC
HpaII 4 CCGG C^CGG
1593 CCGG
MspI 4 CCGG C^CGG
1593 CCGG
NsiI 130 ATGCAT ATGCA^T
535 ATGCAT
NspI 608 ACATGC [GA]CATG^[CT]
1881 GCATGC
RcaI 417 TCATGA T^CATGA
1086 TCATGA
Sau96I 936 GGCCC G^G[ACGT]CC
1595 GGGCC
SspBI 500 TGTACA T^GTACA
1102 TGTACA
StyI 965 CCATGG C^C[AT][AT]GG
1518 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BalI BbvI BcnI BfaI
BsaJI BsiYI BslI BsrI BssKI
BstF5I BstNI BstYI Cac8I DdeI
DpnII DraI EaeI EarI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MvaI MwoI NciI NdeII
NlaIII PleI RsaI Sau3AI ScrFI
SfaNI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII