WASF2 ORF clone FHC03721

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC03721

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Clone info.

Species

human

Clone Name

pFN21AB5891 Sequence (with vector) Restriction Map

Product ID

FHC03721

Flexi Clone PID

FXC03721

ORF Sequence

DNA sequence (1494 bp)
Protein sequence (498 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB527794]

Gene

KIBB5891

Symbol

WASF2

Alias : IMD2, SCAR2, WASF4, WAVE2, dJ393P12.2

Description

WAS protein family, member 2, transcript variant 1

Original Clone ID

cs00591

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1494 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 498 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS304.1 2.3e-62 100.0 WASF2 CCDS55582.1 1.6e-30 99.3 WASF2 CCDS76626.1 1.8e-27 53.5 WASF3 CCDS9318.1 1.5e-25 50.6 WASF3 CCDS5080.1 7.2e-19 48.6 WASF1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003124 436 453 PF02205 Actin-binding WH2 HMMSmart IPR003124 436 453 SM00246 Actin-binding WH2 ProfileScan IPR003124 436 453 PS51082 Actin-binding WH2
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_008921 498 605875 100.0 100.0 NP_001278894 499 605068 53.5 100.0 XP_011533191 502 605068 50.6 100.0 XP_011533192 502 605068 50.6 100.0 NP_006637 502 605068 50.6 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5891 Download

>KIBB5891  1494 bp
ATGCCGTTAGTAACGAGGAACATCGAGCCAAGGCACCTGTGCCGTCAGACGTTGCCTAGC
GTTAGAAGCGAGCTGGAATGCGTGACCAACATCACCCTGGCAAATGTCATCCGACAGCTG
GGCAGCCTGAGTAAATATGCAGAGGACATTTTTGGAGAGCTCTTTACTCAGGCAAATACC
TTTGCCTCTCGGGTAAGCTCCCTTGCTGAGAGGGTCGACCGACTACAGGTTAAAGTCACT
CAGCTGGATCCCAAGGAAGAAGAAGTGTCACTGCAAGGAATCAACACCCGAAAAGCCTTC
AGAAGTTCCACCATTCAAGACCAGAAGCTTTTTGACAGAAACTCTCTCCCAGTGCCTGTC
TTAGAAACATACAATACCTGTGATACTCCTCCCCCTCTCAACAATCTTACCCCTTACAGG
GACGATGGAAAAGAGGCACTCAAATTCTACACAGACCCTTCATACTTCTTTGATCTTTGG
AAGGAGAAGATGCTGCAGGACACCAAGGATATCATGAAAGAGAAGAGAAAGCATAGGAAA
GAAAAGAAAGATAATCCAAATCGAGGGAATGTAAACCCACGTAAAATCAAGACACGTAAG
GAAGAGTGGGAGAAAATGAAGATGGGGCAAGAATTTGTGGAGTCCAAAGAAAAGCTGGGG
ACTTCTGGGTATCCACCCACTTTGGTGTACCAGAATGGCAGCATTGGCTGTGTTGAAAAC
GTGGATGCAAGTAGCTATCCGCCACCACCACAGTCAGACTCTGCTTCTTCACCTTCTCCT
TCCTTCTCCGAGGACAACTTGCCTCCTCCACCAGCAGAATTCAGTTACCCAGTGGACAAC
CAAAGAGGATCTGGTTTGGCTGGACCCAAAAGATCCAGTGTGGTCAGCCCAAGCCATCCA
CCACCAGCTCCTCCTCTAGGCTCTCCACCAGGCCCTAAACCCGGGTTTGCTCCACCACCT
GCCCCTCCGCCACCTCCGCCTCCAATGATAGGCATCCCACCTCCACCACCGCCTGTAGGA
TTTGGGTCTCCAGGGACGCCTCCACCACCCTCACCCCCATCTTTCCCACCTCACCCTGAT
TTTGCTGCCCCTCCACCTCCTCCTCCACCACCAGCAGCTGACTACCCAACTCTGCCACCA
CCTCCCTTGTCCCAGCCAACAGGAGGAGCACCTCCTCCTCCCCCTCCTCCTCCTCCTCCG
GGGCCCCCTCCTCCCCCTTTCACTGGTGCAGATGGCCAGCCTGCTATACCACCACCGCTT
TCTGATACCACCAAGCCCAAGTCCTCCTTGCCTGCCGTGAGCGATGCCCGTAGCGACCTG
CTTTCAGCCATCCGTCAAGGTTTTCAGCTGCGCAGGGTTGAGGAGCAGCGGGAACAAGAG
AAGCGGGATGTTGTGGGCAATGACGTGGCCACCATCTTGTCTCGTCGCATTGCTGTTGAG
TACAGTGACTCAGAAGATGACTCCTCTGAATTTGATGAGGACGACTGGTCCGAT

ORF Protein

Cloned ORF protein sequence for pFN21AB5891 Download

>KIBB5891  498 aa
MPLVTRNIEPRHLCRQTLPSVRSELECVTNITLANVIRQLGSLSKYAEDIFGELFTQANT
FASRVSSLAERVDRLQVKVTQLDPKEEEVSLQGINTRKAFRSSTIQDQKLFDRNSLPVPV
LETYNTCDTPPPLNNLTPYRDDGKEALKFYTDPSYFFDLWKEKMLQDTKDIMKEKRKHRK
EKKDNPNRGNVNPRKIKTRKEEWEKMKMGQEFVESKEKLGTSGYPPTLVYQNGSIGCVEN
VDASSYPPPPQSDSASSPSPSFSEDNLPPPPAEFSYPVDNQRGSGLAGPKRSSVVSPSHP
PPAPPLGSPPGPKPGFAPPPAPPPPPPPMIGIPPPPPPVGFGSPGTPPPPSPPSFPPHPD
FAAPPPPPPPPAADYPTLPPPPLSQPTGGAPPPPPPPPPPGPPPPPFTGADGQPAIPPPL
SDTTKPKSSLPAVSDARSDLLSAIRQGFQLRRVEEQREQEKRDVVGNDVATILSRRIAVE
YSDSEDDSSEFDEDDWSD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5891 Download

>pFN21AB5891  6199 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCGTTAGTAACGAGGAACATCGAGCCAAGGCA
CCTGTGCCGTCAGACGTTGCCTAGCGTTAGAAGCGAGCTGGAATGCGTGACCAACATCAC
CCTGGCAAATGTCATCCGACAGCTGGGCAGCCTGAGTAAATATGCAGAGGACATTTTTGG
AGAGCTCTTTACTCAGGCAAATACCTTTGCCTCTCGGGTAAGCTCCCTTGCTGAGAGGGT
CGACCGACTACAGGTTAAAGTCACTCAGCTGGATCCCAAGGAAGAAGAAGTGTCACTGCA
AGGAATCAACACCCGAAAAGCCTTCAGAAGTTCCACCATTCAAGACCAGAAGCTTTTTGA
CAGAAACTCTCTCCCAGTGCCTGTCTTAGAAACATACAATACCTGTGATACTCCTCCCCC
TCTCAACAATCTTACCCCTTACAGGGACGATGGAAAAGAGGCACTCAAATTCTACACAGA
CCCTTCATACTTCTTTGATCTTTGGAAGGAGAAGATGCTGCAGGACACCAAGGATATCAT
GAAAGAGAAGAGAAAGCATAGGAAAGAAAAGAAAGATAATCCAAATCGAGGGAATGTAAA
CCCACGTAAAATCAAGACACGTAAGGAAGAGTGGGAGAAAATGAAGATGGGGCAAGAATT
TGTGGAGTCCAAAGAAAAGCTGGGGACTTCTGGGTATCCACCCACTTTGGTGTACCAGAA
TGGCAGCATTGGCTGTGTTGAAAACGTGGATGCAAGTAGCTATCCGCCACCACCACAGTC
AGACTCTGCTTCTTCACCTTCTCCTTCCTTCTCCGAGGACAACTTGCCTCCTCCACCAGC
AGAATTCAGTTACCCAGTGGACAACCAAAGAGGATCTGGTTTGGCTGGACCCAAAAGATC
CAGTGTGGTCAGCCCAAGCCATCCACCACCAGCTCCTCCTCTAGGCTCTCCACCAGGCCC
TAAACCCGGGTTTGCTCCACCACCTGCCCCTCCGCCACCTCCGCCTCCAATGATAGGCAT
CCCACCTCCACCACCGCCTGTAGGATTTGGGTCTCCAGGGACGCCTCCACCACCCTCACC
CCCATCTTTCCCACCTCACCCTGATTTTGCTGCCCCTCCACCTCCTCCTCCACCACCAGC
AGCTGACTACCCAACTCTGCCACCACCTCCCTTGTCCCAGCCAACAGGAGGAGCACCTCC
TCCTCCCCCTCCTCCTCCTCCTCCGGGGCCCCCTCCTCCCCCTTTCACTGGTGCAGATGG
CCAGCCTGCTATACCACCACCGCTTTCTGATACCACCAAGCCCAAGTCCTCCTTGCCTGC
CGTGAGCGATGCCCGTAGCGACCTGCTTTCAGCCATCCGTCAAGGTTTTCAGCTGCGCAG
GGTTGAGGAGCAGCGGGAACAAGAGAAGCGGGATGTTGTGGGCAATGACGTGGCCACCAT
CTTGTCTCGTCGCATTGCTGTTGAGTACAGTGACTCAGAAGATGACTCCTCTGAATTTGA
TGAGGACGACTGGTCCGATGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT