Restriction map for pFN21AB5891
 (Linear) (Six-base) MAPSORT of: KIBB5891.seq  Check: 8141  from: 1  to: 1494



KIBB5891.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     966    1494 
   Size:        966     528
 
AccI GT'mk_AC

Cuts at:      0     215    1494 
   Size:        215    1279
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     282    1494 
   Size:        282    1212
 
AflIII A'CryG_T

Cuts at:      0     592    1494 
   Size:        592     902
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1485    1494 
   Size:       1485       9
 
AleI CACnn'nnGTG

Cuts at:      0     682    1494 
   Size:        682     812
 
AlwNI CAG_nnn'CTG

Cuts at:      0     355     760    1117    1494 
   Size:        355     405     357     377

  Fragments arranged by size: 

                 405     377     357     355
 
ApaI G_GGCC'C

Cuts at:      0    1205    1494 
   Size:       1205     289
 
ApoI r'AATT_y

Cuts at:      0     442     631     817    1468    1494 
   Size:        442     189     186     651      26

  Fragments arranged by size: 

                 651     442     189     186      26
 
AvaI C'yCGr_G

Cuts at:      0     188     940    1494 
   Size:        188     752     554

  Fragments arranged by size: 

                 752     554     188
 
BamHI G'GATC_C

Cuts at:      0     246    1494 
   Size:        246    1248
 
BanI G'GyrC_C

Cuts at:      0      32    1494 
   Size:         32    1462
 
BanII G_rGCy'C

Cuts at:      0     161    1205    1494 
   Size:        161    1044     289

  Fragments arranged by size: 

                1044     289     161
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     680    1494 
   Size:        680     814
 
Bme1580I G_kGCm'C

Cuts at:      0    1205    1494 
   Size:       1205     289
 
BmgBI CAC'GTC

Cuts at:      0    1404    1494 
   Size:       1404      90
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     343     823    1494 
   Size:        343     480     671

  Fragments arranged by size: 

                 671     480     343
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1013    1494 
   Size:       1013     481
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1031    1494 
   Size:       1031     463
 
BsaAI yAC'GTr

Cuts at:      0     580     595    1494 
   Size:        580      15     899

  Fragments arranged by size: 

                 899     580      15
 
BsaBI GATnn'nnATC

Cuts at:      0     991    1494 
   Size:        991     503
 
BsaHI Gr'CG_yC

Cuts at:      0    1036    1494 
   Size:       1036     458
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     377     794     899     902    1088    1091    1163    1166 
   Size:        377     417     105       3     186       3      72       3

Cuts at:   1166    1175    1178    1181    1184    1199    1375    1452    1494 
   Size:          9       3       3       3      15     176      77      42

  Fragments arranged by size: 

                 417     377     186     176     105      77      72      42
                  15       9       3       3       3       3       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0     117     654    1151    1494 
   Size:        117     537     497     343

  Fragments arranged by size: 

                 537     497     343     117
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1247    1494 
   Size:       1247     247
 
BsiEI CG_ry'CG

Cuts at:      0     219    1494 
   Size:        219    1275
 
BsiHKAI G_wGCw'C

Cuts at:      0     161    1170    1494 
   Size:        161    1009     324

  Fragments arranged by size: 

                1009     324     161
 
BsmI GAATG_Cn'

Cuts at:      0      82    1494 
   Size:         82    1412
 
Bsp1286I G_dGCh'C

Cuts at:      0     161    1170    1205    1494 
   Size:        161    1009      35     289

  Fragments arranged by size: 

                1009     289     161      35
 
BspHI T'CATG_A

Cuts at:      0     512    1494 
   Size:        512     982
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     966    1325    1494 
   Size:        966     359     169

  Fragments arranged by size: 

                 966     359     169
 
BsrDI GCAATG_nn'

Cuts at:      0    1404    1427    1494 
   Size:       1404      23      67

  Fragments arranged by size: 

                1404      67      23
 
BstYI r'GATC_y

Cuts at:      0     246     847     871    1494 
   Size:        246     601      24     623

  Fragments arranged by size: 

                 623     601     246      24
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1316    1494 
   Size:       1316     178
 
BtsI GCAGTG_nn'

Cuts at:      0     268    1494 
   Size:        268    1226
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     662     697    1127    1162    1494 
   Size:        662      35     430      35     332

  Fragments arranged by size: 

                 662     430     332      35      35
 
EaeI y'GGCC_r

Cuts at:      0    1233    1406    1494 
   Size:       1233     173      88

  Fragments arranged by size: 

                1233     173      88
 
EarI CTCTTCn'nnn_

Cuts at:      0     517     596    1494 
   Size:        517      79     898

  Fragments arranged by size: 

                 898     517      79
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     728     956     965    1494 
   Size:        728     228       9     529

  Fragments arranged by size: 

                 728     529     228       9
 
EcoICRI GAG'CTC

Cuts at:      0     159    1494 
   Size:        159    1335
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     282    1013    1494 
   Size:        282     731     481

  Fragments arranged by size: 

                 731     481     282
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     914    1494 
   Size:        914     580
 
EcoO109I rG'GnC_Cy

Cuts at:      0     931    1201    1202    1494 
   Size:        931     270       1     292

  Fragments arranged by size: 

                 931     292     270       1
 
EcoRI G'AATT_C

Cuts at:      0     817    1494 
   Size:        817     677
 
EcoRV GAT'ATC

Cuts at:      0     510    1494 
   Size:        510     984
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1302    1494 
   Size:       1302     192
 
FspI TGC'GCA

Cuts at:      0    1351    1494 
   Size:       1351     143
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     325     357    1447    1471    1479    1494 
   Size:        325      32    1090      24       8      15

  Fragments arranged by size: 

                1090     325      32      24      15       8
 
HincII GTy'rAC

Cuts at:      0     216    1494 
   Size:        216    1278
 
HindIII A'AGCT_T

Cuts at:      0     325    1494 
   Size:        325    1169
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     135    1494 
   Size:        135    1359
 
MscI TGG'CCA

Cuts at:      0    1235    1408    1494 
   Size:       1235     173      86

  Fragments arranged by size: 

                1235     173      86
 
MslI CAynn'nnrTG

Cuts at:      0     682    1494 
   Size:        682     812
 
MspA1I CmG'CkG

Cuts at:      0     117     243    1117    1347    1368    1494 
   Size:        117     126     874     230      21     126

  Fragments arranged by size: 

                 874     230     126     126     117      21
 
PspOMI G'GGCC_C

Cuts at:      0    1201    1494 
   Size:       1201     293
 
PstI C_TGCA'G

Cuts at:      0     497    1494 
   Size:        497     997
 
PvuII CAG'CTG

Cuts at:      0     117     243    1117    1347    1494 
   Size:        117     126     874     230     147

  Fragments arranged by size: 

                 874     230     147     126     117
 
SacI G_AGCT'C

Cuts at:      0     161    1494 
   Size:        161    1333
 
SalI G'TCGA_C

Cuts at:      0     214    1494 
   Size:        214    1280
 
SfcI C'TryA_G

Cuts at:      0     223     493    1013    1494 
   Size:        223     270     520     481

  Fragments arranged by size: 

                 520     481     270     223
 
SmaI CCC'GGG

Cuts at:      0     942    1494 
   Size:        942     552
 
StyI C'CwwG_G

Cuts at:      0      28     251     503    1494 
   Size:         28     223     252     991

  Fragments arranged by size: 

                 991     252     223      28
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     233     690    1494 
   Size:        233     457     804

  Fragments arranged by size: 

                 804     457     233
 
TatI w'GTAC_w

Cuts at:      0    1439    1494 
   Size:       1439      55
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      93     651     847    1494 
   Size:         93     558     196     647

  Fragments arranged by size: 

                 647     558     196      93
 
XmaI C'CCGG_G

Cuts at:      0     940    1494 
   Size:        940     554
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AhdI     AleI    AlwNI     ApaI
     ApoI     AvaI    BamHI     BanI    BanII    BciVI Bme1580I    BmgBI
     BmrI     BpmI     BsaI    BsaAI    BsaBI    BsaHI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI
    BstYI    BtgZI     BtsI    CspCI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    Hin4I
   HincII  HindIII     MmeI     MscI     MslI   MspA1I   PspOMI     PstI
    PvuII     SacI     SalI     SfcI     SmaI     StyI    TaqII     TatI
     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AlfI     AloI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI     BmtI
     BplI   Bpu10I    BpuEI    BsaWI    BsaXI    BsiWI    BsmBI    BspEI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI     ClaI     DraI   DraIII     DrdI
     EagI     FseI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII    SacII    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XhoI
     XmnI     ZraI
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