Restriction map for
pFN21AB5891
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AvrII BbeI BbrPI
BbsI BbvCI BcgI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoT22I FbaI FseI
HaeII HpaI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 214 GTCGAC GT^[AC][GT]AC
AcyI 1035 GACGCC G[GA]^CG[CT]C
AflIII 592 ACACGT A^C[GA][CT]GT
AhdI 1480 GACGACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1201 GGGCCC GGGCC^C
AspEI 1480 GACGACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1349 TGCGCA TGC^GCA
BamHI 246 GGATCC G^GATCC
BanI 32 GGCACC G^G[CT][GA]CC
BciVI 669 GTATCC GTATCC(6/5)
BpmI 1028 CTCCAG CTGGAG(16/14)
BsaBI 987 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1035 GACGCC G[GA]^CG[CT]C
BsaI 1025 GGTCTC GGTCTC(1/5)
BsgI 1226 GTGCAG GTGCAG(16/14)
BsiEI 216 CGACCG CG[GA][CT]^CG
BsmI 76 GAATGC GAATGC(1/-1)
BspHI 512 TCATGA T^CATGA
CfoI 1350 GCGC GCG^C
Eam1105I 1480 GACGACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 910 CCTCCTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 817 GAATTC G^AATTC
EcoRV 508 GATATC GAT^ATC
FspI 1349 TGCGCA TGC^GCA
HgaI 1035 GACGC GACGC(5/10)
HhaI 1350 GCGC GCG^C
Hin1I 1035 GACGCC G[GA]^CG[CT]C
HinP1I 1350 GCGC G^CGC
HincII 214 GTCGAC GT[CT]^[GA]AC
HindII 214 GTCGAC GT[CT]^[GA]AC
HindIII 325 AAGCTT A^AGCTT
MamI 987 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 230 TTAA T^TAA
MslI 678 CACTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 513 CATG CATG^
PstI 493 CTGCAG CTGCA^G
RcaI 512 TCATGA T^CATGA
SacI 157 GAGCTC GAGCT^C
SalI 214 GTCGAC G^TCGAC
SmaI 940 CCCGGG CCC^GGG
TfiI 278 GAATC G^A[AT]TC
Tru9I 230 TTAA T^TAA
XmaCI 940 CCCGGG C^CCGGG
XmaI 940 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 687 GTAC GT^AC
1440 GTAC
AspHI 157 GAGCTC G[AT]GC[AT]^C
1166 GAGCAC
AvaI 188 CTCGGG C^[CT]CG[GA]G
940 CCCGGG
AvaII 862 GGACC G^G[AT]CC
1487 GGTCC
BalI 1233 TGGCCA TGG^CCA
1406 TGGCCA
BanII 157 GAGCTC G[GA]GC[CT]^C
1201 GGGCCC
BfaI 56 CTAG C^TAG
916 CTAG
BsaAI 578 CACGTA [CT]AC^GT[GA]
593 CACGTA
BsiHKAI 157 GAGCTC G[AT]GC[AT]^C
1166 GAGCAC
BsmAI 1026 GTCTC GTCTC(1/5)
1419 GTCTC
BsoBI 188 CTCGGG C^[CT]CG[GA]G
940 CCCGGG
BspMI 957 ACCTGC ACCTGC(4/8)
1316 ACCTGC
BsrDI 1428 CATTGC GCAATG(2/0)
1397 GCAATG
EaeI 1233 TGGCCA [CT]^GGCC[GA]
1406 TGGCCA
EarI 522 GAAGAG CTCTTC(1/4)
601 GAAGAG
FauI 1368 GCGGG CCCGC(4/6)
1383 GCGGG
HapII 941 CCGG C^CGG
1198 CCGG
HpaII 941 CCGG C^CGG
1198 CCGG
Ksp632I 522 GAAGAG CTCTTC(1/4)
601 GAAGAG
MaeI 56 CTAG C^TAG
916 CTAG
MluNI 1233 TGGCCA TGG^CCA
1406 TGGCCA
MscI 1233 TGGCCA TGG^CCA
1406 TGGCCA
MspI 941 CCGG C^CGG
1198 CCGG
RsaI 687 GTAC GT^AC
1440 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MvaI MwoI NciI NdeII
NlaIV PleI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII