DTX1 ORF clone FHC01256

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC01256

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Clone info.

Species

human

Clone Name

pFN21AB3226 Sequence (with vector) Restriction Map

Product ID

FHC01256

Flexi Clone PID

FXC01256

Original Clone PID

ORK01256

ORF Sequence

DNA sequence (1860 bp)
Protein sequence (620 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384763]

Gene

KIBB3226

Symbol

DTX1

Alias : RNF140, hDx-1

Description

deltex 1, E3 ubiquitin ligase

Original Clone ID

fk00023

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1860 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 620 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9164.1 1.3e-104 100.0 DTX1 CCDS76408.1 6.7e-42 62.4 DTX4 CCDS44612.1 7.6e-42 64.9 DTX4 CCDS5587.1 2.9e-40 48.2 DTX2 CCDS43605.1 5.4e-25 45.8 DTX2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR004170 23 94 PF02825 WWE domain IPR004170 101 171 PF02825 WWE domain HMMSmart IPR018123 23 102 SM00678 WWE domain IPR018123 104 179 SM00678 WWE domain IPR001841 411 471 SM00184 Zinc finger ProfileScan IPR004170 14 94 PS50918 WWE domain IPR004170 95 171 PS50918 WWE domain IPR001841 411 472 PS50089 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011536311 620 602582 100.0 100.0 NP_004407 620 602582 100.0 100.0 NP_055992 619 616110 64.9 95.3 XP_005250189 622 613141 48.2 95.6 NP_001096064 622 613141 48.2 95.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB3226 Download

>KIBB3226  1860 bp
ATGTCACGGCCAGGCCACGGTGGGCTGATGCCTGTGAATGGTCTGGGCTTCCCACCGCAG
AACGTGGCCCGGGTGGTGGTGTGGGAGTGGCTGAATGAGCACAGCCGCTGGCGGCCCTAC
ACGGCCACCGTGTGCCACCACATTGAGAACGTGCTGAAGGAGGACGCTCGCGGTTCCGTG
GTCCTGGGGCAGGTGGACGCCCAGCTTGTGCCCTACATCATCGACCTGCAGTCCATGCAC
CAGTTTCGCCAGGACACAGGCACCATGCGGCCCGTGCGGCGCAACTTCTACGACCCGTCG
TCGGCGCCGGGCAAGGGCATCGTGTGGGAGTGGGAGAACGACGGCGGCGCATGGACGGCC
TACGATATGGACATCTGCATCACCATCCAGAACGCCTACGAGAAGCAGCACCCGTGGCTC
GACCTCTCATCGCTAGGCTTCTGCTACCTCATCTACTTCAACAGCATGTCGCAGATGAAC
CGCCAGACGCGCCGGCGCCGCCGCCTGCGCCGCCGCCTGGACCTCGCCTACCCGCTCACC
GTGGGCTCCATCCCTAAGTCGCAGTCGTGGCCCGTGGGCGCCAGCTCGGGCCAGCCCTGC
TCCTGCCAGCAGTGCCTGCTGGTCAACAGCACGCGCGCCGCCTCCAACGCCATCCTGGCC
TCGCAGCGCCGCAAGGCGCCCCCCGCGCCCCCGCTGCCGCCGCCGCCGCCACCTGGAGGG
CCTCCAGGCGCGCTTGCCGTGCGCCCCAGCGCCACCTTCACAGGCGCCGCGCTCTGGGCA
GCGCCCGCCGCCGGCCCCGCCGAGCCCGCGCCGCCTCCCGGGGCGCCCCCACGGAGCCCG
GGCGCCCCCGGCGGAGCGCGCACCCCGGGGCAGAACAACCTCAACCGGCCCGGGCCCCAG
CGCACCACCAGCGTGAGCGCGCGCGCCTCCATCCCGCCGGGGGTCCCCGCACTCCCGGTG
AAGAACTTGAATGGTACTGGGCCGGTCCATCCGGCCCTGGCAGGGATGACCGGGATACTG
CTGTGCGCGGCCGGGCTGCCCGTGTGCCTGACGCGGGCCCCCAAGCCCATCCTGCACCCG
CCGCCCGTGAGCAAGAGCGACGTGAAGCCCGTGCCTGGCGTGCCCGGGGTGTGCCGCAAG
ACCAAGAAGAAGCACCTTAAAAAGAGTAAGAATCCCGAGGATGTGGTTCGAAGATACATG
CAGAAGGTGAAAAACCCACCTGATGAGGACTGCACCATCTGCATGGAGCGACTGGTCACA
GCATCAGGCTACGAGGGCGTGCTTCGGCACAAGGGCGTGCGGCCTGAGCTCGTGGGCCGC
CTGGGCCGCTGTGGCCACATGTACCACCTGCTGTGCCTCGTGGCCATGTACTCCAATGGC
AACAAGGATGGCAGCCTGCAGTGCCCCACCTGCAAGGCCATCTACGGGGAGAAGACGGGT
ACGCAGCCGCCTGGGAAGATGGAGTTCCACCTCATCCCCCACTCGCTGCCCGGCTTCCCT
GATACCCAGACCATCCGCATCGTCTATGACATCCCCACAGGCATCCAGGGCCCTGAGCAC
CCCAACCCCGGGAAGAAGTTCACCGCAAGAGGATTCCCTCGCCACTGCTATCTACCCAAC
AACGAGAAAGGCCGGAAGGTGCTGCGGCTGCTCATCACGGCCTGGGAGAGAAGACTCATC
TTCACTATCGGCACGTCCAACACCACGGGCGAGTCGGACACCGTGGTGTGGAACGAGATC
CACCACAAGACCGAGTTTGGATCCAACCTCACGGGCCACGGCTACCCGGACGCTAGCTAC
CTAGACAACGTGCTGGCTGAGCTCACAGCCCAGGGCGTATCCGAGGCTGCAGCCAAGGCT

ORF Protein

Cloned ORF protein sequence for pFN21AB3226 Download

>KIBB3226  620 aa
MSRPGHGGLMPVNGLGFPPQNVARVVVWEWLNEHSRWRPYTATVCHHIENVLKEDARGSV
VLGQVDAQLVPYIIDLQSMHQFRQDTGTMRPVRRNFYDPSSAPGKGIVWEWENDGGAWTA
YDMDICITIQNAYEKQHPWLDLSSLGFCYLIYFNSMSQMNRQTRRRRRLRRRLDLAYPLT
VGSIPKSQSWPVGASSGQPCSCQQCLLVNSTRAASNAILASQRRKAPPAPPLPPPPPPGG
PPGALAVRPSATFTGAALWAAPAAGPAEPAPPPGAPPRSPGAPGGARTPGQNNLNRPGPQ
RTTSVSARASIPPGVPALPVKNLNGTGPVHPALAGMTGILLCAAGLPVCLTRAPKPILHP
PPVSKSDVKPVPGVPGVCRKTKKKHLKKSKNPEDVVRRYMQKVKNPPDEDCTICMERLVT
ASGYEGVLRHKGVRPELVGRLGRCGHMYHLLCLVAMYSNGNKDGSLQCPTCKAIYGEKTG
TQPPGKMEFHLIPHSLPGFPDTQTIRIVYDIPTGIQGPEHPNPGKKFTARGFPRHCYLPN
NEKGRKVLRLLITAWERRLIFTIGTSNTTGESDTVVWNEIHHKTEFGSNLTGHGYPDASY
LDNVLAELTAQGVSEAAAKA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB3226 Download

>pFN21AB3226  6565 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCACGGCCAGGCCACGGTGGGCTGATGCCTGT
GAATGGTCTGGGCTTCCCACCGCAGAACGTGGCCCGGGTGGTGGTGTGGGAGTGGCTGAA
TGAGCACAGCCGCTGGCGGCCCTACACGGCCACCGTGTGCCACCACATTGAGAACGTGCT
GAAGGAGGACGCTCGCGGTTCCGTGGTCCTGGGGCAGGTGGACGCCCAGCTTGTGCCCTA
CATCATCGACCTGCAGTCCATGCACCAGTTTCGCCAGGACACAGGCACCATGCGGCCCGT
GCGGCGCAACTTCTACGACCCGTCGTCGGCGCCGGGCAAGGGCATCGTGTGGGAGTGGGA
GAACGACGGCGGCGCATGGACGGCCTACGATATGGACATCTGCATCACCATCCAGAACGC
CTACGAGAAGCAGCACCCGTGGCTCGACCTCTCATCGCTAGGCTTCTGCTACCTCATCTA
CTTCAACAGCATGTCGCAGATGAACCGCCAGACGCGCCGGCGCCGCCGCCTGCGCCGCCG
CCTGGACCTCGCCTACCCGCTCACCGTGGGCTCCATCCCTAAGTCGCAGTCGTGGCCCGT
GGGCGCCAGCTCGGGCCAGCCCTGCTCCTGCCAGCAGTGCCTGCTGGTCAACAGCACGCG
CGCCGCCTCCAACGCCATCCTGGCCTCGCAGCGCCGCAAGGCGCCCCCCGCGCCCCCGCT
GCCGCCGCCGCCGCCACCTGGAGGGCCTCCAGGCGCGCTTGCCGTGCGCCCCAGCGCCAC
CTTCACAGGCGCCGCGCTCTGGGCAGCGCCCGCCGCCGGCCCCGCCGAGCCCGCGCCGCC
TCCCGGGGCGCCCCCACGGAGCCCGGGCGCCCCCGGCGGAGCGCGCACCCCGGGGCAGAA
CAACCTCAACCGGCCCGGGCCCCAGCGCACCACCAGCGTGAGCGCGCGCGCCTCCATCCC
GCCGGGGGTCCCCGCACTCCCGGTGAAGAACTTGAATGGTACTGGGCCGGTCCATCCGGC
CCTGGCAGGGATGACCGGGATACTGCTGTGCGCGGCCGGGCTGCCCGTGTGCCTGACGCG
GGCCCCCAAGCCCATCCTGCACCCGCCGCCCGTGAGCAAGAGCGACGTGAAGCCCGTGCC
TGGCGTGCCCGGGGTGTGCCGCAAGACCAAGAAGAAGCACCTTAAAAAGAGTAAGAATCC
CGAGGATGTGGTTCGAAGATACATGCAGAAGGTGAAAAACCCACCTGATGAGGACTGCAC
CATCTGCATGGAGCGACTGGTCACAGCATCAGGCTACGAGGGCGTGCTTCGGCACAAGGG
CGTGCGGCCTGAGCTCGTGGGCCGCCTGGGCCGCTGTGGCCACATGTACCACCTGCTGTG
CCTCGTGGCCATGTACTCCAATGGCAACAAGGATGGCAGCCTGCAGTGCCCCACCTGCAA
GGCCATCTACGGGGAGAAGACGGGTACGCAGCCGCCTGGGAAGATGGAGTTCCACCTCAT
CCCCCACTCGCTGCCCGGCTTCCCTGATACCCAGACCATCCGCATCGTCTATGACATCCC
CACAGGCATCCAGGGCCCTGAGCACCCCAACCCCGGGAAGAAGTTCACCGCAAGAGGATT
CCCTCGCCACTGCTATCTACCCAACAACGAGAAAGGCCGGAAGGTGCTGCGGCTGCTCAT
CACGGCCTGGGAGAGAAGACTCATCTTCACTATCGGCACGTCCAACACCACGGGCGAGTC
GGACACCGTGGTGTGGAACGAGATCCACCACAAGACCGAGTTTGGATCCAACCTCACGGG
CCACGGCTACCCGGACGCTAGCTACCTAGACAACGTGCTGGCTGAGCTCACAGCCCAGGG
CGTATCCGAGGCTGCAGCCAAGGCTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT