Restriction map for
pFN21AB3226
##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspI AviII AvrII
BbrPI BbvCI BclI BfrI BglII
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI Bst1107I BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI EarI Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MunI NcoI NdeI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp509I Tth111I Van91I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 1337 ACATGT A^C[GA][CT]GT
Asp700I 1572 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1759 GGATCC G^GATCC
BlpI 1816 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1816 GCTGAGC GC^T[ACGT]AGC
BsiEI 1028 CGGCCG CG[GA][CT]^CG
BsmFI 943 GTCCC GGGAC(10/14)
BspLU11I 1337 ACATGT A^CATGT
BsrBI 532 CCGCTC CCGCTC(-3/-3)
BstBI 1187 TTCGAA TT^CGAA
BstXI 1235 CCATCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1816 GCTGAGC GC^T[ACGT]AGC
DraIII 126 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1028 CGGCCG C^GGCCG
EclXI 1028 CGGCCG C^GGCCG
Eco52I 1028 CGGCCG C^GGCCG
EcoNI 183 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1853 CCAAGG C^C[AT][AT]GG
HincII 622 GTCAAC GT[CT]^[GA]AC
HindII 622 GTCAAC GT[CT]^[GA]AC
MseI 1157 TTAA T^TAA
NheI 1792 GCTAGC G^CTAGC
NspV 1187 TTCGAA TT^CGAA
PpuMI 941 GGGTCCC [GA]G^G[AT]CC[CT]
SfuI 1187 TTCGAA TT^CGAA
SgrAI 490 CGCCGGCG C[GA]^CCGG[CT]G
StyI 1853 CCAAGG C^C[AT][AT]GG
Tru9I 1157 TTAA T^TAA
XcmI 644 CCAACGCCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1572 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AhdI 223 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
292 GACCCGTCGTC
AlwNI 102 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
610 CAGTGCCTG
AspEI 223 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
292 GACCCGTCGTC
BalI 1332 TGGCCA TGG^CCA
1361 TGGCCA
BbsI 1431 GAAGAC GAAGAC(2/6)
1670 GAAGAC
BcgI 237 GCACCAGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
311 GCAAGGGCATCG
BciVI 1013 GGATAC GTATCC(6/5)
1837 GTATCC
BpmI 722 CTCCAG CTGGAG(16/14)
713 CTGGAG
BpuAI 1431 GAAGAC GAAGAC(2/6)
1670 GAAGAC
BsgI 1072 CTGCAC GTGCAG(16/14)
1230 CTGCAC
BssSI 1309 CTCGTG CACGAG(-5/-1)
1357 CTCGTG
BstYI 1736 AGATCC [GA]^GATC[CT]
1759 GGATCC
DpnII 1737 GATC ^GATC
1760 GATC
Eam1105I 223 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
292 GACCCGTCGTC
MaeIII 3 GTCAC ^GT[ACGT]AC
1255 GTCAC
MboI 1737 GATC ^GATC
1760 GATC
MflI 1736 AGATCC [GA]^GATC[CT]
1759 GGATCC
MluNI 1332 TGGCCA TGG^CCA
1361 TGGCCA
MscI 1332 TGGCCA TGG^CCA
1361 TGGCCA
NaeI 491 GCCGGC GCC^GGC
790 GCCGGC
NdeII 1737 GATC ^GATC
1760 GATC
NgoMIV 491 GCCGGC G^CCGGC
790 GCCGGC
PleI 1673 GACTC GAGTC(4/5)
1711 GAGTC
SacI 1306 GAGCTC GAGCT^C
1819 GAGCTC
Sau3AI 1737 GATC ^GATC
1760 GATC
SfiI 1315 GGCCGCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1324 GGCCGCTGTGGCC
TfiI 1170 GAATC G^A[AT]TC
1592 GATTC
Tsp45I 3 GTCAC ^GT[GC]AC
1255 GTCAC
XhoII 1736 AGATCC [GA]^GATC[CT]
1759 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BfaI BglI BmyI BsaHI BsaJI
BsiHKAI BsiYI BslI BsoBI Bsp1286I
BspMI BsrFI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeI
MaeII MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NlaIII NlaIV NspI PstI
RsaI Sau96I ScrFI SfaNI SfcI
SfoI SmaI TaqI TseI TspRI
TthHB8I XmaCI XmaI