(Circular) (Six-base) MAPSORT of: pFN21AB3226.seq Check: 6454 from: 1 to: 6565 pFN21AB3226 6565 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2185 3360 3422 2185 Size: 1175 62 5328 Fragments arranged by size: 5328 1175 62 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4375 278 Size: 53 83 186 936 2839 2468 Fragments arranged by size: 2839 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3908 1937 Size: 1971 4594 Acc65I G'GTAC_C Cuts at: 3886 3886 Size: 6565 AclI AA'CG_TT Cuts at: 1563 4692 5065 1563 Size: 3129 373 3063 Fragments arranged by size: 3129 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2180 4628 6012 6280 1632 Size: 548 2448 1384 268 1917 Fragments arranged by size: 2448 1917 1384 548 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6368 AflIII A'CryG_T Cuts at: 1203 3342 5464 6441 1203 Size: 2139 2122 977 1327 Fragments arranged by size: 2139 2122 1327 977 AgeI A'CCGG_T Cuts at: 5396 5396 Size: 6565 AhdI GACnn_n'nnGTC Cuts at: 2233 2302 5294 2233 Size: 69 2992 3504 Fragments arranged by size: 3504 2992 69 AleI CACnn'nnGTG Cuts at: 1166 2915 3728 1166 Size: 1749 813 4003 Fragments arranged by size: 4003 1749 813 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6565 AlwNI CAG_nnn'CTG Cuts at: 2112 2620 4308 5880 2112 Size: 508 1688 1572 2797 Fragments arranged by size: 2797 1688 1572 508 ApaI G_GGCC'C Cuts at: 2901 3064 3557 2901 Size: 163 493 5909 Fragments arranged by size: 5909 493 163 ApaLI G'TGCA_C Cuts at: 4622 5778 4622 Size: 1156 5409 ApoI r'AATT_y Cuts at: 1493 3874 4100 4252 6361 1493 Size: 2381 226 152 2109 1697 Fragments arranged by size: 2381 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5119 160 Size: 872 4087 1606 Fragments arranged by size: 4087 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6565 AvaI C'yCGr_G Cuts at: 1870 1943 2073 2590 2822 2842 2869 2894 3128 Size: 73 130 517 232 20 27 25 234 Cuts at: 3128 3179 3572 3890 1870 Size: 51 393 318 4545 Fragments arranged by size: 4545 517 393 318 234 232 130 73 51 27 25 20 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3011 3044 3011 Size: 33 6532 BamHI G'GATC_C Cuts at: 3764 3895 4262 3764 Size: 131 367 6067 Fragments arranged by size: 6067 367 131 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2264 2308 2499 2582 2680 Size: 325 217 627 477 44 191 83 98 Cuts at: 2680 2768 2827 2846 3886 5341 618 Size: 88 59 19 1040 1455 1842 Fragments arranged by size: 1842 1455 1040 627 477 325 217 191 98 88 83 59 44 19 BanII G_rGCy'C Cuts at: 1391 2552 2811 2843 2901 3064 3315 3557 3828 Size: 1161 259 32 58 163 251 242 271 Cuts at: 3828 3884 1391 Size: 56 4072 Fragments arranged by size: 4072 1161 271 259 251 242 163 58 56 32 BbeI G_GCGC'C Cuts at: 2312 2503 2586 2684 2772 2831 2850 2312 Size: 191 83 98 88 59 19 6027 Fragments arranged by size: 6027 191 98 88 83 59 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3443 3682 928 Size: 971 1544 239 3811 Fragments arranged by size: 3811 1544 971 239 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2231 2265 2305 2339 4756 4790 1903 Size: 34 294 34 40 34 2417 34 3678 Fragments arranged by size: 3678 2417 294 40 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3012 3853 4457 5667 3012 Size: 841 604 1210 3910 Fragments arranged by size: 3910 1210 841 604 BclI T'GATC_A Cuts at: 1548 1548 Size: 6565 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2281 2660 3327 Size: 107 122 71 631 605 609 379 667 Cuts at: 3327 3336 5176 136 Size: 9 1840 1525 Fragments arranged by size: 1840 1525 667 631 609 605 379 122 107 71 9 BglII A'GATC_T Cuts at: 6560 6560 Size: 6565 BlpI GC'TnA_GC Cuts at: 3822 3982 3822 Size: 160 6405 Bme1580I G_kGCm'C Cuts at: 1790 2217 2901 3064 3129 3410 3557 4626 5782 Size: 427 684 163 65 281 147 1069 1156 Cuts at: 5782 1790 Size: 2573 Fragments arranged by size: 2573 1156 1069 684 427 281 163 147 65 BmgBI CAC'GTC Cuts at: 1318 3106 3699 1318 Size: 1788 593 4184 Fragments arranged by size: 4184 1788 593 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2991 4296 5254 Size: 416 53 83 399 24 1574 1305 958 Cuts at: 5254 442 Size: 1753 Fragments arranged by size: 1753 1574 1305 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3801 1056 Size: 2745 3820 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2712 2739 5225 1239 Size: 1473 27 2486 2579 Fragments arranged by size: 2579 2486 1473 27 Bpu10I CC'TnA_GC Cuts at: 3309 3558 3309 Size: 249 6316 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4690 5555 5853 4690 Size: 865 298 5402 Fragments arranged by size: 5402 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5228 882 Size: 617 3729 2219 Fragments arranged by size: 3729 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4356 493 Size: 711 3152 2702 Fragments arranged by size: 3152 2702 711 BsaBI GATnn'nnATC Cuts at: 6559 6559 Size: 6565 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2202 2309 2500 2583 Size: 53 83 186 936 669 107 191 83 Cuts at: 2583 2681 2769 2828 2847 4372 4754 5389 275 Size: 98 88 59 19 1525 382 635 1451 Fragments arranged by size: 1525 1451 936 669 635 382 191 186 107 98 88 83 83 59 53 19 BsaWI w'CCGG_w Cuts at: 4998 5396 5670 5817 6228 4998 Size: 398 274 147 411 5335 Fragments arranged by size: 5335 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6340 Fragments arranged by size: 6340 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6565 BseYI C'CCAG_C Cuts at: 2205 2750 2901 5768 6264 2205 Size: 545 151 2867 496 2506 Fragments arranged by size: 2867 2506 545 496 151 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3062 3220 1163 Size: 573 1326 158 4508 Fragments arranged by size: 4508 1326 573 158 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3036 4015 4776 5804 1540 Size: 275 186 1035 979 761 1028 2301 Fragments arranged by size: 2301 1035 1028 979 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2106 3315 3564 3828 4626 4711 5782 2106 Size: 1209 249 264 798 85 1071 2889 Fragments arranged by size: 2889 1209 1071 798 264 249 85 BsmI GAATG_Cn' Cuts at: 4076 4169 4076 Size: 93 6472 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2217 2552 2811 2843 2901 3064 Size: 399 316 111 335 259 32 58 163 Cuts at: 3064 3129 3315 3410 3557 3564 3828 3884 4626 Size: 65 186 95 147 7 264 56 742 Cuts at: 4626 4711 5782 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 335 316 264 259 186 163 147 111 95 85 65 58 56 32 7 BspEI T'CCGG_A Cuts at: 6228 6228 Size: 6565 BspHI T'CATG_A Cuts at: 4454 6184 4454 Size: 1730 4835 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2185 2238 3360 3422 3920 844 Size: 1341 53 1122 62 498 3489 Fragments arranged by size: 3489 1341 1122 498 62 53 BsrBI CCG'CTC Cuts at: 1394 1823 2539 4452 1394 Size: 429 716 1913 3507 Fragments arranged by size: 3507 1913 716 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5060 5234 65 Size: 1183 3812 174 1396 Fragments arranged by size: 3812 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2496 2795 2889 2986 5209 5396 1700 Size: 110 686 299 94 97 2223 187 2869 Fragments arranged by size: 2869 2223 686 299 187 110 97 94 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6565 BssHII G'CGCG_C Cuts at: 1925 2638 2733 2861 2922 2924 2926 1925 Size: 713 95 128 61 2 2 5564 Fragments arranged by size: 5564 713 128 95 61 2 2 BssSI C'ACGA_G Cuts at: 3314 3362 4625 5637 3314 Size: 48 1263 1012 4242 Fragments arranged by size: 4242 1263 1012 48 BstAPI GCAn_nnn'nTGC Cuts at: 794 2239 2620 3243 794 Size: 1445 381 623 4116 Fragments arranged by size: 4116 1445 623 381 BstBI TT'CG_AA Cuts at: 3193 5369 3193 Size: 2176 4389 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6565 BstXI CCAn_nnnn'nTGG Cuts at: 3247 3247 Size: 6565 BstYI r'GATC_y Cuts at: 1972 3741 3764 3895 4262 4647 4664 6116 6202 Size: 1769 23 131 367 385 17 1452 86 Cuts at: 6202 6214 6560 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1769 1452 385 367 346 131 86 23 17 12 BtgI C'CryG_G Cuts at: 513 1065 2020 2181 2417 2544 2577 2834 3708 Size: 552 955 161 236 127 33 257 874 Cuts at: 3708 3726 3781 513 Size: 18 55 3297 Fragments arranged by size: 3297 955 874 552 257 236 161 127 55 33 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2418 487 Size: 683 56 1192 4634 Fragments arranged by size: 4634 1192 683 56 BtsI GCAGTG_nn' Cuts at: 2621 3410 3607 4081 4875 4895 2621 Size: 789 197 474 794 20 4291 Fragments arranged by size: 4291 794 789 474 197 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6565 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6530 DraI TTT'AAA Cuts at: 3869 3869 Size: 6565 DraIII CAC_nnn'GTG Cuts at: 2136 2136 Size: 6565 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5572 809 Size: 507 4256 1802 Fragments arranged by size: 4256 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2012 2127 3033 Size: 54 1425 50 275 18 182 115 906 Cuts at: 3033 3337 3366 4012 4901 6530 8 Size: 304 29 646 889 1629 43 Fragments arranged by size: 1629 1425 906 889 646 304 275 182 115 54 50 43 29 18 EagI C'GGCC_G Cuts at: 1537 1812 3033 4012 1537 Size: 275 1221 979 4090 Fragments arranged by size: 4090 1221 979 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4495 1353 Size: 3142 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2871 5152 5526 5672 387 Size: 1064 138 1282 2281 374 146 1280 Fragments arranged by size: 2281 1282 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3313 3826 3313 Size: 513 6052 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2180 2712 2739 4628 5225 6012 6280 Size: 393 548 532 27 1889 597 787 268 Cuts at: 6280 1239 Size: 1524 Fragments arranged by size: 1889 1524 787 597 548 532 393 268 27 EcoNI CCTnn'n_nnAGG Cuts at: 2192 2192 Size: 6565 EcoO109I rG'GnC_Cy Cuts at: 2723 2898 2947 3061 3553 3554 2723 Size: 175 49 114 492 1 5734 Fragments arranged by size: 5734 492 175 114 49 1 EcoRI G'AATT_C Cuts at: 3874 4252 3874 Size: 378 6187 FspI TGC'GCA Cuts at: 5071 5071 Size: 6565 HaeII r_GCGC'y Cuts at: 1678 2312 2503 2586 2674 2684 2757 2772 2789 Size: 634 191 83 88 10 73 15 17 Cuts at: 2789 2831 2850 5712 1678 Size: 42 19 2862 2531 Fragments arranged by size: 2862 2531 634 191 88 83 73 42 19 17 15 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2219 2251 2288 2320 2417 Size: 32 135 32 647 32 37 32 97 Cuts at: 2417 2449 3160 3192 3749 3756 3781 3788 5206 Size: 32 711 32 557 7 25 7 1418 Cuts at: 5206 5238 5280 5312 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1418 711 647 557 135 97 42 37 32 32 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2629 3909 4155 669 Size: 988 281 691 1280 246 3079 Fragments arranged by size: 3079 1280 988 691 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6565 HpaI GTT'AAC Cuts at: 4155 4155 Size: 6565 KasI G'GCGC_C Cuts at: 2308 2499 2582 2680 2768 2827 2846 2308 Size: 191 83 98 88 59 19 6027 Fragments arranged by size: 6027 191 98 88 83 59 19 KpnI G_GTAC'C Cuts at: 3890 3890 Size: 6565 MfeI C'AATT_G Cuts at: 4164 4164 Size: 6565 MluI A'CGCG_T Cuts at: 6441 6441 Size: 6565 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2673 3699 3726 3792 5679 5863 1305 Size: 1368 1026 27 66 1887 184 2007 Fragments arranged by size: 2007 1887 1368 1026 184 66 27 MscI TGG'CCA Cuts at: 10 64 1489 1832 3339 3368 10 Size: 54 1425 343 1507 29 3207 Fragments arranged by size: 3207 1507 1425 343 54 29 MslI CAynn'nnrTG Cuts at: 518 1166 2915 3245 3728 4523 4882 5041 518 Size: 648 1749 330 483 795 359 159 2042 Fragments arranged by size: 2042 1749 795 648 483 359 330 159 MspA1I CmG'CkG Cuts at: 1606 2112 2698 3333 3981 4658 5806 6051 1606 Size: 506 586 635 648 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 648 635 586 506 245 NaeI GCC'GGC Cuts at: 2498 2797 2498 Size: 299 6266 NarI GG'CG_CC Cuts at: 2309 2500 2583 2681 2769 2828 2847 2309 Size: 191 83 98 88 59 19 6027 Fragments arranged by size: 6027 191 98 88 83 59 19 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6013 NdeI CA'TA_TG Cuts at: 387 387 Size: 6565 NgoMIV G'CCGG_C Cuts at: 2496 2795 2496 Size: 299 6266 NheI G'CTAG_C Cuts at: 1052 3797 1052 Size: 2745 3820 NotI GC'GGCC_GC Cuts at: 4012 4012 Size: 6565 NspI r_CATG'y Cuts at: 1132 1229 2473 3205 3346 3923 5468 1132 Size: 97 1244 732 141 577 1545 2229 Fragments arranged by size: 2229 1545 1244 732 577 141 97 PasI CC'CwG_GG Cuts at: 1348 3835 1348 Size: 2487 4078 PciI A'CATG_T Cuts at: 3342 5464 3342 Size: 2122 4443 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6565 PmeI GTTT'AAAC Cuts at: 3869 3869 Size: 6565 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4634 4666 6147 6179 4634 Size: 32 1481 32 5020 Fragments arranged by size: 5020 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2947 2947 Size: 6565 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6565 PsiI TTA'TAA Cuts at: 4135 4135 Size: 6565 PspOMI G'GGCC_C Cuts at: 2897 3060 3553 2897 Size: 163 493 5909 Fragments arranged by size: 5909 493 163 PstI C_TGCA'G Cuts at: 830 2235 3405 3856 3917 830 Size: 1405 1170 451 61 3478 Fragments arranged by size: 3478 1405 1170 451 61 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6565 SacI G_AGCT'C Cuts at: 3315 3828 3315 Size: 513 6052 SalI G'TCGA_C Cuts at: 1936 3907 1936 Size: 1971 4594 SanDI GG'GwC_CC Cuts at: 2947 2947 Size: 6565 SbfI CC_TGCA'GG Cuts at: 3917 3917 Size: 6565 ScaI AGT'ACT Cuts at: 4813 6525 4813 Size: 1712 4853 SfcI C'TryA_G Cuts at: 826 1045 2231 3401 3852 3913 5048 5729 5920 Size: 219 1186 1170 451 61 1135 681 191 Cuts at: 5920 826 Size: 1471 Fragments arranged by size: 1471 1186 1170 1135 681 451 219 191 61 SfiI GGCCn_nnn'nGGCC Cuts at: 3327 3336 3327 Size: 9 6556 SfoI GGC'GCC Cuts at: 2310 2501 2584 2682 2770 2829 2848 2310 Size: 191 83 98 88 59 19 6027 Fragments arranged by size: 6027 191 98 88 83 59 19 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6565 SgrAI Cr'CCGG_yG Cuts at: 1700 2496 1700 Size: 796 5769 SmaI CCC'GGG Cuts at: 1872 2075 2824 2844 2871 2896 3130 3574 3892 Size: 203 749 20 27 25 234 444 318 Cuts at: 3892 1872 Size: 4545 Fragments arranged by size: 4545 749 444 318 234 203 27 25 20 SmlI C'TyrA_G Cuts at: 820 1017 1943 4669 5570 5832 820 Size: 197 926 2726 901 262 1553 Fragments arranged by size: 2726 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4356 493 Size: 3863 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6565 SphI G_CATG'C Cuts at: 1132 3923 1132 Size: 2791 3774 SrfI GCCC'GGGC Cuts at: 2844 2896 2844 Size: 52 6513 SspI AAT'ATT Cuts at: 5 52 4489 5 Size: 47 4437 2081 Fragments arranged by size: 4437 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6565 StyI C'CwwG_G Cuts at: 513 1065 3858 4004 513 Size: 552 2793 146 3074 Fragments arranged by size: 3074 2793 552 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3770 4592 4609 4762 4947 5392 325 Size: 1255 2190 822 17 153 185 445 1498 Fragments arranged by size: 2190 1498 1255 822 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3372 4811 6523 Size: 274 80 33 51 1443 1395 1439 1712 Cuts at: 6523 96 Size: 138 Fragments arranged by size: 1712 1443 1439 1395 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6525 6557 6525 Size: 32 6533 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6565 XbaI T'CTAG_A Cuts at: 3901 3901 Size: 6565 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2656 2656 Size: 6565 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6565 XmaI C'CCGG_G Cuts at: 1870 2073 2822 2842 2869 2894 3128 3572 3890 Size: 203 749 20 27 25 234 444 318 Cuts at: 3890 1870 Size: 4545 Fragments arranged by size: 4545 749 444 318 234 203 27 25 20 XmnI GAAnn'nnTTC Cuts at: 3581 4694 3581 Size: 1113 5452 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4373 276 Size: 53 83 186 936 2839 2468 Fragments arranged by size: 2839 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SapI SexAI SwaI