ZBTB24 ORF clone FHC00537

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00537

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Clone info.

Species

human

Clone Name

pFN21ASDA0441 Sequence (with vector) Restriction Map

Product ID

FHC00537

Flexi Clone PID

FXC00537

ORF Sequence

DNA sequence (2091 bp)
Protein sequence (697 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB383884]

Gene

KIAA0441

Symbol

ZBTB24

Alias : BIF1, ICF2, PATZ2, ZNF450

Description

zinc finger and BTB domain containing 24, transcript variant 1

Original Clone ID

hh00601s1

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2091 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 697 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS34509.1 2.2e-159 99.9 ZBTB24 CCDS35075.1 2.4e-26 42.7 ZNF782 CCDS74388.1 2.2e-25 47.4 ZNF227 CCDS12636.1 2.3e-25 47.4 ZNF227 CCDS58688.1 3.8e-25 45.7 ZNF211 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013069 27 131 PF00651 BTB/POZ IPR007087 434 456 PF00096 Zinc finger HMMSmart IPR000210 37 133 SM00225 BTB/POZ-like IPR015880 294 316 SM00355 Zinc finger IPR015880 322 344 SM00355 Zinc finger IPR015880 350 372 SM00355 Zinc finger IPR015880 378 400 SM00355 Zinc finger IPR015880 406 428 SM00355 Zinc finger IPR015880 434 456 SM00355 Zinc finger IPR015880 462 484 SM00355 Zinc finger IPR015880 490 512 SM00355 Zinc finger ProfileScan IPR000210 37 103 PS50097 BTB/POZ-like IPR007087 294 321 PS50157 Zinc finger IPR007087 322 349 PS50157 Zinc finger IPR007087 350 377 PS50157 Zinc finger IPR007087 378 405 PS50157 Zinc finger IPR007087 406 433 PS50157 Zinc finger IPR007087 434 461 PS50157 Zinc finger IPR007087 462 489 PS50157 Zinc finger IPR007087 490 517 PS50157 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055612 697 614064,614069 99.9 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0441 Download

>KIAA0441  2091 bp
ATGGCAGAAACATCGCCAGAGCCTTCTGGGCAGCTTGTTGTACACTCAGACGCTCACAGT
GACACTGTGCTGGCCAGTTTTGAGGATCAGAGGAAGAAAGGCTTCCTCTGTGACATTACT
TTAATCGTGGAGAATGTACATTTCCGGGCCCACAAAGCCTTACTTGCTGCCAGTAGTGAA
TACTTCTCAATGATGTTTGCAGAAGAGGGGGAAATCGGCCAATCCATTTATATGCTGGAA
GGCATGGTTGCAGACACCTTTGGTATCCTGCTGGAATTTATCTACACAGGTTATCTCCAT
GCCAGTGAGAAAAGTACAGAACAAATCCTGGCTACTGCTCAGTTCTTAAAAGTCTATGAC
CTGGTAAAGGCTTACACAGACTTCCAAAATAATCATAGCTCCCCAAAGCCAACAACTTTG
AACACTGCTGGTGCCCCAGTGGTTGTTATCCCTAATAAGAAAAACGATCCTCCAAAGCGG
AAACGGGGAAGACCAAAAAAAGTCAATACATTGCAGGAGGAGAAATCAGAACTGGCTGCA
GAGGAAGAAATACAGTTAAGAGTGAACAATTCAGTTCAGAATAGACAAAACTTTGTGGTT
AAAGGAGACAGTGGTGTACTGAATGAGCAAATTGCAGCAAAAGAAAAGGAAGAATCGGAG
CCTACTTGTGAGCCAAGTAGAGAGGAGGAAATGCCAGTTGAAAAAGATGAGAACTATGAT
CCCAAGACCGAGGATGGCCAGGCAAGCCAGAGTCGATACAGCAAGCGGAGGATTTGGAGA
TCCGTCAAACTTAAAGATTACAAACTTGTTGGGGATCAAGAGGACCATGGTTCAGCCAAG
AGGATCTGTGGAAGGAGAAAGCGCCCTGGAGGCCCTGAGGCCCGCTGTAAAGACTGTGGC
AAGGTCTTTAAGTACAATCACTTTTTAGCAATCCACCAGAGGAGCCACACAGGGGAGCGA
CCTTTCAAATGTAATGAGTGTGGAAAAGGCTTTGCCCAGAAGCACTCGCTACAGGTCCAC
ACCAGGATGCACACAGGCGAGCGGCCGTACACCTGCACCGTGTGCAGCAAGGCTCTGACC
ACCAAGCACTCACTGCTGGAGCACATGAGCCTGCACTCAGGACAGAAGTCTTTTACCTGT
GATCAATGCGGAAAATATTTCAGCCAGAACAGACAGCTAAAGAGCCATTACCGAGTTCAT
ACAGGCCACTCATTACCGGAATGCAAAGACTGCCATCGCAAATTCATGGATGTGTCTCAG
CTAAAGAAACATCTGCGAACACACACAGGTGAGAAGCCATTTACTTGTGAAATCTGTGGC
AAATCTTTCACAGCAAAGAGTTCTCTTCAGACCCACATCAGAATCCATCGAGGAGAAAAG
CCATACTCCTGTGGCATTTGTGGCAAATCCTTCTCTGACTCCAGTGCCAAAAGGAGACAC
TGCATTCTACACACTGGCAAGAAGCCTTTCTCCTGCCCTGAGTGTAACTTACAGTTTGCT
CGCTTAGACAACTTGAAGGCTCACTTGAAAATTCATAGCAAGGAGAAGCATGCTTCAGAT
GCCAGCAGCATTTCTGGCAGTAGTAATACAGAAGAGGTCAGGAATATTCTTCAGCTACAG
CCATATCAACTCTCTACCTCGGGAGAGCAGGAAATTCAGCTTCTCGTAACCGATTCTGTA
CATAACATCAATTTCATGCCCGGTCCTAGCCAGGGAATCAGCATTGTGACTGCAGAGAGT
TCCCAAAACATGACTGCAGACCAGGCTGCTAATCTTACCCTGCTCACGCAGCAGCCAGAG
CAACTGCAGAATTTAATTCTTTCAGCTCAACAGGAGCAAACAGAACACATTCAGAGCCTC
AATATGATTGAAAGCCAGATGGGGCCCTCACAAACAGAGCCAGTGCACGTGATCACTCTG
TCCAAGGAAACACTGGAACATCTTCATGCCCATCAAGAGCAAACAGAGGAGCTCCATTTA
GCTACAAGTACTTCAGATCCAGCTCAGCACCTGCAGCTGACACAGGAGCCTGGTCCGCCA
CCACCCACTCACCACGTGCCCCAGCCAACGCCACTTGGCCAGGAGCAGAGC

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0441 Download

>KIAA0441  697 aa
MAETSPEPSGQLVVHSDAHSDTVLASFEDQRKKGFLCDITLIVENVHFRAHKALLAASSE
YFSMMFAEEGEIGQSIYMLEGMVADTFGILLEFIYTGYLHASEKSTEQILATAQFLKVYD
LVKAYTDFQNNHSSPKPTTLNTAGAPVVVIPNKKNDPPKRKRGRPKKVNTLQEEKSELAA
EEEIQLRVNNSVQNRQNFVVKGDSGVLNEQIAAKEKEESEPTCEPSREEEMPVEKDENYD
PKTEDGQASQSRYSKRRIWRSVKLKDYKLVGDQEDHGSAKRICGRRKRPGGPEARCKDCG
KVFKYNHFLAIHQRSHTGERPFKCNECGKGFAQKHSLQVHTRMHTGERPYTCTVCSKALT
TKHSLLEHMSLHSGQKSFTCDQCGKYFSQNRQLKSHYRVHTGHSLPECKDCHRKFMDVSQ
LKKHLRTHTGEKPFTCEICGKSFTAKSSLQTHIRIHRGEKPYSCGICGKSFSDSSAKRRH
CILHTGKKPFSCPECNLQFARLDNLKAHLKIHSKEKHASDASSISGSSNTEEVRNILQLQ
PYQLSTSGEQEIQLLVTDSVHNINFMPGPSQGISIVTAESSQNMTADQAANLTLLTQQPE
QLQNLILSAQQEQTEHIQSLNMIESQMGPSQTEPVHVITLSKETLEHLHAHQEQTEELHL
ATSTSDPAQHLQLTQEPGPPPPTHHVPQPTPLGQEQS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0441 Download

>pFN21ASDA0441  6820 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGCAGAAACATCGCC
AGAGCCTTCTGGGCAGCTTGTTGTACACTCAGACGCTCACAGTGACACTGTGCTGGCCAG
TTTTGAGGATCAGAGGAAGAAAGGCTTCCTCTGTGACATTACTTTAATCGTGGAGAATGT
ACATTTCCGGGCCCACAAAGCCTTACTTGCTGCCAGTAGTGAATACTTCTCAATGATGTT
TGCAGAAGAGGGGGAAATCGGCCAATCCATTTATATGCTGGAAGGCATGGTTGCAGACAC
CTTTGGTATCCTGCTGGAATTTATCTACACAGGTTATCTCCATGCCAGTGAGAAAAGTAC
AGAACAAATCCTGGCTACTGCTCAGTTCTTAAAAGTCTATGACCTGGTAAAGGCTTACAC
AGACTTCCAAAATAATCATAGCTCCCCAAAGCCAACAACTTTGAACACTGCTGGTGCCCC
AGTGGTTGTTATCCCTAATAAGAAAAACGATCCTCCAAAGCGGAAACGGGGAAGACCAAA
AAAAGTCAATACATTGCAGGAGGAGAAATCAGAACTGGCTGCAGAGGAAGAAATACAGTT
AAGAGTGAACAATTCAGTTCAGAATAGACAAAACTTTGTGGTTAAAGGAGACAGTGGTGT
ACTGAATGAGCAAATTGCAGCAAAAGAAAAGGAAGAATCGGAGCCTACTTGTGAGCCAAG
TAGAGAGGAGGAAATGCCAGTTGAAAAAGATGAGAACTATGATCCCAAGACCGAGGATGG
CCAGGCAAGCCAGAGTCGATACAGCAAGCGGAGGATTTGGAGATCCGTCAAACTTAAAGA
TTACAAACTTGTTGGGGATCAAGAGGACCATGGTTCAGCCAAGAGGATCTGTGGAAGGAG
AAAGCGCCCTGGAGGCCCTGAGGCCCGCTGTAAAGACTGTGGCAAGGTCTTTAAGTACAA
TCACTTTTTAGCAATCCACCAGAGGAGCCACACAGGGGAGCGACCTTTCAAATGTAATGA
GTGTGGAAAAGGCTTTGCCCAGAAGCACTCGCTACAGGTCCACACCAGGATGCACACAGG
CGAGCGGCCGTACACCTGCACCGTGTGCAGCAAGGCTCTGACCACCAAGCACTCACTGCT
GGAGCACATGAGCCTGCACTCAGGACAGAAGTCTTTTACCTGTGATCAATGCGGAAAATA
TTTCAGCCAGAACAGACAGCTAAAGAGCCATTACCGAGTTCATACAGGCCACTCATTACC
GGAATGCAAAGACTGCCATCGCAAATTCATGGATGTGTCTCAGCTAAAGAAACATCTGCG
AACACACACAGGTGAGAAGCCATTTACTTGTGAAATCTGTGGCAAATCTTTCACAGCAAA
GAGTTCTCTTCAGACCCACATCAGAATCCATCGAGGAGAAAAGCCATACTCCTGTGGCAT
TTGTGGCAAATCCTTCTCTGACTCCAGTGCCAAAAGGAGACACTGCATTCTACACACTGG
CAAGAAGCCTTTCTCCTGCCCTGAGTGTAACTTACAGTTTGCTCGCTTAGACAACTTGAA
GGCTCACTTGAAAATTCATAGCAAGGAGAAGCATGCTTCAGATGCCAGCAGCATTTCTGG
CAGTAGTAATACAGAAGAGGTCAGGAATATTCTTCAGCTACAGCCATATCAACTCTCTAC
CTCGGGAGAGCAGGAAATTCAGCTTCTCGTAACCGATTCTGTACATAACATCAATTTCAT
GCCCGGTCCTAGCCAGGGAATCAGCATTGTGACTGCAGAGAGTTCCCAAAACATGACTGC
AGACCAGGCTGCTAATCTTACCCTGCTCACGCAGCAGCCAGAGCAACTGCAGAATTTAAT
TCTTTCAGCTCAACAGGAGCAAACAGAACACATTCAGAGCCTCAATATGATTGAAAGCCA
GATGGGGCCCTCACAAACAGAGCCAGTGCACGTGATCACTCTGTCCAAGGAAACACTGGA
ACATCTTCATGCCCATCAAGAGCAAACAGAGGAGCTCCATTTAGCTACAAGTACTTCAGA
TCCAGCTCAGCACCTGCAGCTGACACAGGAGCCTGGTCCGCCACCACCCACTCACCACGT
GCCCCAGCCAACGCCACTTGGCCAGGAGCAGAGCTACGTAGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT