(Circular) (Six-base) MAPSORT of: pFN21ASDA0441.seq Check: 1365 from: 1 to: 6820 pFN21ASDA0441 6820 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3083 4041 3083 Size: 958 5862 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4630 278 Size: 53 83 186 936 3094 2468 Fragments arranged by size: 3094 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4163 1937 Size: 2226 4594 Acc65I G'GTAC_C Cuts at: 4141 4141 Size: 6820 AclI AA'CG_TT Cuts at: 1563 4947 5320 1563 Size: 3384 373 3063 Fragments arranged by size: 3384 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3353 3561 3617 3999 4883 6267 6535 1632 Size: 1721 208 56 382 884 1384 268 1917 Fragments arranged by size: 1917 1721 1384 884 382 268 208 56 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6623 AflIII A'CryG_T Cuts at: 1203 5719 6696 1203 Size: 4516 977 1327 Fragments arranged by size: 4516 1327 977 AgeI A'CCGG_T Cuts at: 5651 5651 Size: 6820 AhdI GACnn_n'nnGTC Cuts at: 5549 5549 Size: 6820 AleI CACnn'nnGTG Cuts at: 1166 3309 1166 Size: 2143 4677 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6820 AlwNI CAG_nnn'CTG Cuts at: 4033 4563 6135 4033 Size: 530 1572 4718 Fragments arranged by size: 4718 1572 530 ApaI G_GGCC'C Cuts at: 2173 3909 2173 Size: 1736 5084 ApaLI G'TGCA_C Cuts at: 3926 4877 6033 3926 Size: 951 1156 4713 Fragments arranged by size: 4713 1156 951 ApoI r'AATT_y Cuts at: 1493 2297 3263 3552 3675 3832 4129 4355 4507 Size: 804 966 289 123 157 297 226 152 Cuts at: 4507 6616 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 966 804 297 289 226 157 152 123 AseI AT'TA_AT Cuts at: 160 1032 5374 160 Size: 872 4342 1606 Fragments arranged by size: 4342 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6820 AvaI C'yCGr_G Cuts at: 1870 1943 3661 4145 1870 Size: 73 1718 484 4545 Fragments arranged by size: 4545 1718 484 73 BamHI G'GATC_C Cuts at: 4150 4517 4150 Size: 367 6453 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2453 4141 5596 618 Size: 325 217 627 666 1688 1455 1842 Fragments arranged by size: 1842 1688 1455 666 627 325 217 BanII G_rGCy'C Cuts at: 1391 2173 3909 3996 4139 1391 Size: 782 1736 87 143 4072 Fragments arranged by size: 4072 1736 782 143 87 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2518 928 Size: 971 619 5230 Fragments arranged by size: 5230 971 619 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2016 2050 5011 5045 1903 Size: 34 79 34 2961 34 3678 Fragments arranged by size: 3678 2961 79 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2297 4712 5922 2297 Size: 2415 1210 3195 Fragments arranged by size: 3195 2415 1210 BclI T'GATC_A Cuts at: 1548 3163 3933 1548 Size: 1615 770 4435 Fragments arranged by size: 4435 1615 770 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2050 2892 5431 Size: 107 122 71 631 605 378 842 2539 Cuts at: 5431 136 Size: 1525 Fragments arranged by size: 2539 1525 842 631 605 378 122 107 71 BglII A'GATC_T Cuts at: 6815 6815 Size: 6820 BlpI GC'TnA_GC Cuts at: 4026 4237 4026 Size: 211 6609 Bme1580I G_kGCm'C Cuts at: 1790 2173 2458 3909 3930 4083 4881 6037 1790 Size: 383 285 1451 21 153 798 1156 2573 Fragments arranged by size: 2573 1451 1156 798 383 285 153 21 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6820 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2453 4551 5509 Size: 416 53 83 399 24 1036 2098 958 Cuts at: 5509 442 Size: 1753 Fragments arranged by size: 2098 1753 1036 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6820 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2910 3140 3427 5480 1239 Size: 1671 230 287 2053 2579 Fragments arranged by size: 2579 2053 1671 287 230 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4945 5810 6108 4945 Size: 865 298 5657 Fragments arranged by size: 5657 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5483 882 Size: 617 3984 2219 Fragments arranged by size: 3984 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3931 4078 4117 4611 493 Size: 711 2727 147 39 494 2702 Fragments arranged by size: 2727 2702 711 494 147 39 BsaBI GATnn'nnATC Cuts at: 6814 6814 Size: 6820 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4627 5009 5644 275 Size: 53 83 186 936 3094 382 635 1451 Fragments arranged by size: 3094 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3238 5253 5651 5925 6072 6483 3238 Size: 2015 398 274 147 411 3575 Fragments arranged by size: 3575 2015 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6595 Fragments arranged by size: 6595 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2555 2720 2977 3408 4004 1186 Size: 1369 165 257 431 596 4002 Fragments arranged by size: 4002 1369 596 431 257 165 BseYI C'CCAG_C Cuts at: 4083 6023 6519 4083 Size: 1940 496 4384 Fragments arranged by size: 4384 1940 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3061 3106 3119 1163 Size: 573 1325 45 13 4864 Fragments arranged by size: 4864 1325 573 45 13 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3068 4270 5031 6059 1540 Size: 275 186 1067 1202 761 1028 2301 Fragments arranged by size: 2301 1202 1067 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3126 3930 3996 4881 4966 6037 3126 Size: 804 66 885 85 1071 3909 Fragments arranged by size: 3909 1071 885 804 85 66 BsmI GAATG_Cn' Cuts at: 3248 3465 4331 4424 3248 Size: 217 866 93 5644 Fragments arranged by size: 5644 866 217 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2173 2458 3126 3909 3930 3996 4083 Size: 399 383 285 668 783 21 66 87 Cuts at: 4083 4139 4881 4966 6037 1391 Size: 56 742 85 1071 2174 Fragments arranged by size: 2174 1071 783 742 668 399 383 285 87 85 66 56 21 BspEI T'CCGG_A Cuts at: 6483 6483 Size: 6820 BspHI T'CATG_A Cuts at: 4709 6439 4709 Size: 1730 5090 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3083 4041 4175 844 Size: 2239 958 134 3489 Fragments arranged by size: 3489 2239 958 134 BsrBI CCG'CTC Cuts at: 1394 1823 3064 4707 1394 Size: 429 1241 1643 3507 Fragments arranged by size: 3507 1643 1241 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2531 5315 5489 65 Size: 1183 1283 2784 174 1396 Fragments arranged by size: 2784 1396 1283 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5464 5651 1700 Size: 110 3654 187 2869 Fragments arranged by size: 3654 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2062 2158 3700 96 Size: 1966 96 1542 3216 Fragments arranged by size: 3216 1966 1542 96 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6820 BssSI C'ACGA_G Cuts at: 4880 5892 4880 Size: 1012 5808 BstAPI GCAn_nnn'nTGC Cuts at: 794 3084 3115 3252 794 Size: 2290 31 137 4362 Fragments arranged by size: 4362 2290 137 31 BstBI TT'CG_AA Cuts at: 2007 5624 2007 Size: 3617 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6820 BstYI r'GATC_y Cuts at: 1972 2801 2865 4018 4150 4517 4902 4919 6371 Size: 829 64 1153 132 367 385 17 1452 Cuts at: 6371 6457 6469 6815 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1153 829 385 367 346 132 86 64 17 12 Bsu36I CC'TnA_GG Cuts at: 2898 2898 Size: 6820 BtgI C'CryG_G Cuts at: 513 1065 2022 2848 513 Size: 552 957 826 4485 Fragments arranged by size: 4485 957 826 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2019 3242 487 Size: 683 56 793 1223 4065 Fragments arranged by size: 4065 1223 793 683 56 BtsI GCAGTG_nn' Cuts at: 2445 3113 3460 4336 5130 5150 2445 Size: 668 347 876 794 20 4115 Fragments arranged by size: 4115 876 794 668 347 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6820 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6785 DraI TTT'AAA Cuts at: 4124 4124 Size: 6820 DraIII CAC_nnn'GTG Cuts at: 3084 4078 3084 Size: 994 5826 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5827 809 Size: 507 4511 1802 Fragments arranged by size: 4511 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2239 2758 Size: 54 1425 50 275 18 264 145 519 Cuts at: 2758 3065 4099 4267 5156 6785 8 Size: 307 1034 168 889 1629 43 Fragments arranged by size: 1629 1425 1034 889 519 307 275 264 168 145 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3065 4267 1537 Size: 275 1253 1202 4090 Fragments arranged by size: 4090 1253 1202 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2220 3372 3609 4750 1353 Size: 867 1152 237 1141 3423 Fragments arranged by size: 3423 1152 1141 867 237 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4047 5407 5781 5927 387 Size: 1064 138 2458 1360 374 146 1280 Fragments arranged by size: 2458 1360 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3994 3994 Size: 6820 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2910 3140 3353 3427 3561 3617 3999 Size: 393 1278 230 213 74 134 56 382 Cuts at: 3999 4883 5480 6267 6535 1239 Size: 884 597 787 268 1524 Fragments arranged by size: 1524 1278 884 787 597 393 382 268 230 213 134 74 56 EcoNI CCTnn'n_nnAGG Cuts at: 2387 2387 Size: 6820 EcoO109I rG'GnC_Cy Cuts at: 2894 3905 3906 2894 Size: 1011 1 5808 Fragments arranged by size: 5808 1011 1 EcoRI G'AATT_C Cuts at: 4129 4507 4129 Size: 378 6442 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2140 2201 3562 2140 Size: 61 1361 5398 Fragments arranged by size: 5398 1361 61 FspI TGC'GCA Cuts at: 5326 5326 Size: 6820 HaeII r_GCGC'y Cuts at: 1678 2887 5967 1678 Size: 1209 3080 2531 Fragments arranged by size: 3080 2531 1209 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2531 2563 5461 5493 5535 Size: 32 135 32 959 32 2898 32 42 Cuts at: 5535 5567 1373 Size: 32 2626 Fragments arranged by size: 2898 2626 959 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4164 4410 669 Size: 988 281 2226 246 3079 Fragments arranged by size: 3079 2226 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6820 HpaI GTT'AAC Cuts at: 4410 4410 Size: 6820 KpnI G_GTAC'C Cuts at: 4145 4145 Size: 6820 MfeI C'AATT_G Cuts at: 4419 4419 Size: 6820 MluI A'CGCG_T Cuts at: 6696 6696 Size: 6820 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5934 6118 1305 Size: 4629 184 2007 Fragments arranged by size: 4629 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2096 2760 4101 10 Size: 54 1425 343 264 664 1341 2729 Fragments arranged by size: 2729 1425 1341 664 343 264 54 MslI CAynn'nnrTG Cuts at: 518 1166 2252 2325 3047 3272 3309 3966 4778 Size: 648 1086 73 722 225 37 657 812 Cuts at: 4778 5137 5296 518 Size: 359 159 2042 Fragments arranged by size: 2042 1086 812 722 657 648 359 225 159 73 37 MspA1I CmG'CkG Cuts at: 1606 2907 4039 4236 4913 6061 6306 1606 Size: 1301 1132 197 677 1148 245 2120 Fragments arranged by size: 2120 1301 1148 1132 677 245 197 NcoI C'CATG_G Cuts at: 513 1065 2022 2848 513 Size: 552 957 826 4485 Fragments arranged by size: 4485 957 826 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6820 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6820 NotI GC'GGCC_GC Cuts at: 4267 4267 Size: 6820 NspI r_CATG'y Cuts at: 1132 1229 3575 4178 5723 1132 Size: 97 2346 603 1545 2229 Fragments arranged by size: 2346 2229 1545 603 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6820 PciI A'CATG_T Cuts at: 5719 5719 Size: 6820 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6820 PmeI GTTT'AAAC Cuts at: 4124 4124 Size: 6820 PmlI CAC'GTG Cuts at: 3931 4078 3931 Size: 147 6673 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4889 4921 6402 6434 4889 Size: 32 1481 32 5275 Fragments arranged by size: 5275 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3148 1613 Size: 1535 5285 PsiI TTA'TAA Cuts at: 4390 4390 Size: 6820 PspOMI G'GGCC_C Cuts at: 2169 3905 2169 Size: 1736 5084 PstI C_TGCA'G Cuts at: 830 2563 3757 3781 3831 4038 4172 830 Size: 1733 1194 24 50 207 134 3478 Fragments arranged by size: 3478 1733 1194 207 134 50 24 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6820 PvuII CAG'CTG Cuts at: 4039 4039 Size: 6820 SacI G_AGCT'C Cuts at: 3996 3996 Size: 6820 SalI G'TCGA_C Cuts at: 1936 4162 1936 Size: 2226 4594 SbfI CC_TGCA'GG Cuts at: 4172 4172 Size: 6820 ScaI AGT'ACT Cuts at: 4012 5068 6780 4012 Size: 1056 1712 4052 Fragments arranged by size: 4052 1712 1056 SexAI A'CCwGG_T Cuts at: 2382 2382 Size: 6820 SfcI C'TryA_G Cuts at: 826 1045 2559 3032 3638 3753 3777 3827 4034 Size: 219 1514 473 606 115 24 50 207 Cuts at: 4034 4168 5303 5984 6175 826 Size: 134 1135 681 191 1471 Fragments arranged by size: 1514 1471 1135 681 606 473 219 207 191 134 115 50 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6820 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6820 SmaI CCC'GGG Cuts at: 1872 4147 1872 Size: 2275 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4924 5825 6087 820 Size: 197 926 2981 901 262 1553 Fragments arranged by size: 2981 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4117 4611 493 Size: 3624 494 2702 Fragments arranged by size: 3624 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6820 SphI G_CATG'C Cuts at: 1132 3575 4178 1132 Size: 2443 603 3774 Fragments arranged by size: 3774 2443 603 SspI AAT'ATT Cuts at: 5 52 3179 3628 4744 5 Size: 47 3127 449 1116 2081 Fragments arranged by size: 3127 2081 1116 449 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6820 StyI C'CwwG_G Cuts at: 513 1065 2022 2848 3945 4259 513 Size: 552 957 826 1097 314 3074 Fragments arranged by size: 3074 1097 957 826 552 314 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2765 4081 4847 4864 5017 5202 5647 Size: 1255 1185 1316 766 17 153 185 445 Cuts at: 5647 325 Size: 1498 Fragments arranged by size: 1498 1316 1255 1185 766 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2062 2158 2336 Size: 274 80 33 51 1443 85 96 178 Cuts at: 2336 2638 2934 3700 4010 5066 6778 96 Size: 302 296 766 310 1056 1712 138 Fragments arranged by size: 1712 1443 1056 766 310 302 296 274 178 138 96 85 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6780 6812 6780 Size: 32 6788 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6820 XbaI T'CTAG_A Cuts at: 4156 4156 Size: 6820 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2254 3952 2254 Size: 1698 5122 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6820 XmaI C'CCGG_G Cuts at: 1870 4145 1870 Size: 2275 4545 XmnI GAAnn'nnTTC Cuts at: 2123 3488 3870 3963 4949 2123 Size: 1365 382 93 986 3994 Fragments arranged by size: 3994 1365 986 382 93 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4628 276 Size: 53 83 186 936 3094 2468 Fragments arranged by size: 3094 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI