TRUB1 ORF clone FHC09335

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC09335

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Clone info.

Species

human

Clone Name

pFN21AE5409 Sequence (with vector) Restriction Map

Product ID

FHC09335

ORF Sequence

DNA sequence (1047 bp)
Protein sequence (349 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5409

Symbol

TRUB1

Alias : PUS4

Description

TruB pseudouridine (psi) synthase family member 1

Original Clone ID

oc95409

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1047 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 349 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7591.1 5e-130 100.0 TRUB1 CCDS6897.1 1.5e-17 34.1 TRUB2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR002501 106 255 PF01509 Pseudouridine synthase II HMMTigr IPR014780 70 289 TIGR00431 tRNA pseudouridine synthase II
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_631908 349 610726 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5409 Download

>KIEE5409  1047 bp
ATGGCCGCTTCTGAGGCGGCGGTGGTGTCTTCGCCGTCTTTGAAAACAGACACATCCCCT
GTCCTTGAAACTGCAGGAACGGTCGCAGCAATGGCTGCGACCCCGTCAGCAAGGGCTGCA
GCCGCGGTGGTTGCGGCCGCGGCCAGGACCGGATCCGAAGCCAGGGTCTCCAAGGCCGCT
TTGGCTACCAAGCTGCTGTCCTTGAGCGGCGTGTTCGCCGTGCACAAGCCCAAAGGGCCC
ACTTCAGCCGAGCTGCTGAATCGGTTGAAGGAGAAGCTGCTGGCAGAAGCTGGAATGCCT
TCTCCAGAATGGACCAAGAGGAAAAAGCAGACTTTGAAAATTGGGCATGGAGGGACTCTA
GACAGCGCAGCCCGAGGAGTTCTGGTTGTTGGAATTGGAAGCGGAACAAAAATGTTGACC
AGTATGTTGTCAGGGTCCAAGAGATATACTGCCATTGGAGAACTGGGGAAAGCTACTGAT
ACACTAGATTCTACGGGGAGGGTAACAGAAGAAAAACCTTACGATAAAATAACACAAGAA
GATATTGAAGGCATTCTACAGAAATTTACTGGAAATATAATGCAAGTGCCCCCCCTCTAT
TCTGCATTAAAGAAAGATGGACAAAGACTTTCGACTTTGATGAAGAGAGGTGAAGTCGTA
GAAGCAAAACCTGCCAGGCCAGTGACTGTATACAGTATCTCCCTTCAAAAATTCCAGCCA
CCATTTTTCACATTAGATGTTGAATGTGGAGGAGGTTTTTATATCAGAAGCTTGGTCAGT
GACATTGGAAAAGAACTATCTTCCTGTGCCAATGTGCTAGAGCTGACCCGAACCAAACAG
GGACCATTTACGCTAGAAGAACATGCCCTTCCTGAAGACAAATGGACAATTGATGACATT
GCACAGTCTCTTGAGCATTGCTCATCTCTTTTCCCAGCAGAGTTGGCACTTAAAAAATCA
AAACCTGAGTCTAATGAACAGGTTTTGAGCTGTGAATATATAACTCTAAATGAGCCAAAG
AGAGAAGATGATGTAATTAAGACGTGT

ORF Protein

Cloned ORF protein sequence for pFN21AE5409 Download

>KIEE5409  349 aa
MAASEAAVVSSPSLKTDTSPVLETAGTVAAMAATPSARAAAAVVAAAARTGSEARVSKAA
LATKLLSLSGVFAVHKPKGPTSAELLNRLKEKLLAEAGMPSPEWTKRKKQTLKIGHGGTL
DSAARGVLVVGIGSGTKMLTSMLSGSKRYTAIGELGKATDTLDSTGRVTEEKPYDKITQE
DIEGILQKFTGNIMQVPPLYSALKKDGQRLSTLMKRGEVVEAKPARPVTVYSISLQKFQP
PFFTLDVECGGGFYIRSLVSDIGKELSSCANVLELTRTKQGPFTLEEHALPEDKWTIDDI
AQSLEHCSSLFPAELALKKSKPESNEQVLSCEYITLNEPKREDDVIKTC

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5409 Download

>pFN21AE5409  5752 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCGCTTCTGAGGCGGCGGTGGTGTCTTCGCC
GTCTTTGAAAACAGACACATCCCCTGTCCTTGAAACTGCAGGAACGGTCGCAGCAATGGC
TGCGACCCCGTCAGCAAGGGCTGCAGCCGCGGTGGTTGCGGCCGCGGCCAGGACCGGATC
CGAAGCCAGGGTCTCCAAGGCCGCTTTGGCTACCAAGCTGCTGTCCTTGAGCGGCGTGTT
CGCCGTGCACAAGCCCAAAGGGCCCACTTCAGCCGAGCTGCTGAATCGGTTGAAGGAGAA
GCTGCTGGCAGAAGCTGGAATGCCTTCTCCAGAATGGACCAAGAGGAAAAAGCAGACTTT
GAAAATTGGGCATGGAGGGACTCTAGACAGCGCAGCCCGAGGAGTTCTGGTTGTTGGAAT
TGGAAGCGGAACAAAAATGTTGACCAGTATGTTGTCAGGGTCCAAGAGATATACTGCCAT
TGGAGAACTGGGGAAAGCTACTGATACACTAGATTCTACGGGGAGGGTAACAGAAGAAAA
ACCTTACGATAAAATAACACAAGAAGATATTGAAGGCATTCTACAGAAATTTACTGGAAA
TATAATGCAAGTGCCCCCCCTCTATTCTGCATTAAAGAAAGATGGACAAAGACTTTCGAC
TTTGATGAAGAGAGGTGAAGTCGTAGAAGCAAAACCTGCCAGGCCAGTGACTGTATACAG
TATCTCCCTTCAAAAATTCCAGCCACCATTTTTCACATTAGATGTTGAATGTGGAGGAGG
TTTTTATATCAGAAGCTTGGTCAGTGACATTGGAAAAGAACTATCTTCCTGTGCCAATGT
GCTAGAGCTGACCCGAACCAAACAGGGACCATTTACGCTAGAAGAACATGCCCTTCCTGA
AGACAAATGGACAATTGATGACATTGCACAGTCTCTTGAGCATTGCTCATCTCTTTTCCC
AGCAGAGTTGGCACTTAAAAAATCAAAACCTGAGTCTAATGAACAGGTTTTGAGCTGTGA
ATATATAACTCTAAATGAGCCAAAGAGAGAAGATGATGTAATTAAGACGTGTGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT