################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AfaI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsgI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII BssSI BstBI BstEII BstPI BstXI Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HpaI KasI KpnI MamI MluI MluNI MroI MscI MslI NaeI NarI NciI NcoI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccI 688 GTATAC GT^[AC][GT]AC AflIII 1042 ACGTGT A^C[GA][CT]GT Alw44I 220 GTGCAC G^TGCAC ApaI 235 GGGCCC GGGCC^C ApaLI 220 GTGCAC G^TGCAC AspHI 220 GTGCAC G[AT]GC[AT]^C AspI 99 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 371 CCCGAG C^[CT]CG[GA]G BamHI 151 GGATCC G^GATCC BanII 235 GGGCCC G[GA]GC[CT]^C BpmI 302 CTCCAG CTGGAG(16/14) BsaI 165 GGTCTC GGTCTC(1/5) BsaWI 148 ACCGGA [AT]^CCGG[AT] BsiHKAI 220 GTGCAC G[AT]GC[AT]^C BsoBI 371 CCCGAG C^[CT]CG[GA]G BspMI 669 ACCTGC ACCTGC(4/8) BsrBI 204 GAGCGG CCGCTC(-3/-3) Bst1107I 688 GTATAC GTA^TAC BstF5I 53 CATCC GGATG(2/0) BstYI 151 GGATCC [GA]^GATC[CT] BstZ17I 688 GTATAC GTA^TAC Cac8I 279 GCTGGC GC[ACGT]^[ACGT]GC CfoI 365 GCGC GCG^C DpnII 152 GATC ^GATC DraII 234 AGGGCCC [GA]G^G[ACGT]CC[CT] EagI 134 CGGCCG C^GGCCG EarI 642 GAAGAG CTCTTC(1/4) EclXI 134 CGGCCG C^GGCCG Eco52I 134 CGGCCG C^GGCCG EcoO109I 234 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoT14I 170 CCAAGG C^C[AT][AT]GG FokI 53 CATCC GGATG(9/13) HapII 149 CCGG C^CGG HhaI 365 GCGC GCG^C HinP1I 365 GCGC G^CGC HincII 414 GTTGAC GT[CT]^[GA]AC HindII 414 GTTGAC GT[CT]^[GA]AC HindIII 768 AAGCTT A^AGCTT HpaII 149 CCGG C^CGG HphI 649 GGTGA GGTGA(8/7) Ksp632I 642 GAAGAG CTCTTC(1/4) MaeII 1042 ACGT A^CGT MboI 152 GATC ^GATC MfeI 887 CAATTG C^AATTG MflI 151 GGATCC [GA]^GATC[CT] MspI 149 CCGG C^CGG MunI 887 CAATTG C^AATTG NdeII 152 GATC ^GATC NotI 133 GCGGCCGC GC^GGCCGC NspI 861 ACATGC [GA]CATG^[CT] PflFI 99 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC Sau3AI 152 GATC ^GATC StyI 170 CCAAGG C^C[AT][AT]GG TaqI 631 TCGA T^CGA Tth111I 99 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 631 TCGA T^CGA XbaI 357 TCTAGA T^CTAGA XhoII 151 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 123 CGCG CG^CG 138 CGCG AcsI 562 AAATTT [GA]^AATT[CT] 709 AAATTC AlwI 152 GATCC GGATC(4/5) 151 GGATC AlwNI 284 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 680 CAGTGACTG ApoI 562 AAATTT [GA]^AATT[CT] 709 AAATTC Asp700I 293 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 547 GAAGGCATTC BbsI 874 GAAGAC GAAGAC(2/6) 27 GTCTTC BglI 175 GCCGCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 228 GCCCAAAGGGC BpuAI 874 GAAGAC GAAGAC(2/6) 27 GTCTTC BseRI 374 GAGGAG GAGGAG(10/8) 749 GAGGAG BsiEI 80 CGGTCG CG[GA][CT]^CG 134 CGGCCG BsiYI 137 CCGCGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 155 CCGAAGCCAGG BslI 137 CCGCGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 155 CCGAAGCCAGG BsmAI 166 GTCTC GTCTC(1/5) 906 GTCTC BsmFI 352 GGGAC GGGAC(10/14) 840 GGGAC BsmI 293 GAATGC GAATGC(1/-1) 551 GCATTC BstUI 123 CGCG CG^CG 138 CGCG DdeI 11 CTGAG C^T[ACGT]AG 965 CTGAG DsaI 122 CCGCGG C^C[GA][CT]GG 137 CCGCGG KspI 122 CCGCGG CCGC^GG 137 CCGCGG MspA1I 122 CCGCGG C[AC]G^C[GT]G 137 CCGCGG MvnI 123 CGCG CG^CG 138 CGCG NlaIII 346 CATG CATG^ 862 CATG PleI 354 GACTC GAGTC(4/5) 967 GAGTC PstI 71 CTGCAG CTGCA^G 116 CTGCAG SacII 122 CCGCGG CCGC^GG 137 CCGCGG SmlI 201 CTTGAG C^T[CT][GA]AG 910 CTTGAG TfiI 258 GAATC G^A[AT]TC 487 GATTC Tsp45I 682 GTGAC ^GT[GC]AC 779 GTGAC TspRI 680 CAGTG CA[GC]TG[ACGT][ACGT]^ 777 CAGTG XcmI 170 CCAAGGCCGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 452 CCATTGGAGAACTGG XmnI 293 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 547 GAAGGCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AciI AluI AvaII BbvI BfaI BmyI BsaJI Bsp1286I BsrDI BsrI BssKI BstAPI BstNI Cfr13I EaeI EcoRII Fnu4HI HaeIII HinfI ItaI MaeI MaeIII MboII MnlI MseI MvaI MwoI NlaIV Sau96I ScrFI SfcI Tru9I TseI Tsp509I