GLI4 ORF clone FHC08726

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC08726

How to Order Order Information

Clone info.

Species

human

Clone Name

pFN21AE1053 Sequence (with vector) Restriction Map

Product ID

FHC08726

ORF Sequence

DNA sequence (1128 bp)
Protein sequence (376 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590236]

Gene

KIEE1053

Symbol

GLI4

Alias : HKR4, ZNF928

Description

GLI family zinc finger 4

Original Clone ID

cp01520

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1128 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 376 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS6398.1 1e-89 100.0 GLI4 CCDS43619.1 4.6e-30 47.6 ZNF3 CCDS74472.1 2.1e-29 54.5 ZNF135 CCDS74471.1 2.1e-29 54.5 ZNF135 CCDS12970.2 2.2e-29 54.5 ZNF135 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR007087 183 205 PF00096 Zinc finger IPR007087 239 261 PF00096 Zinc finger IPR007087 267 289 PF00096 Zinc finger IPR007087 323 345 PF00096 Zinc finger HMMSmart IPR015880 183 205 SM00355 Zinc finger IPR015880 211 233 SM00355 Zinc finger IPR015880 239 261 SM00355 Zinc finger IPR015880 267 289 SM00355 Zinc finger IPR015880 295 317 SM00355 Zinc finger IPR015880 323 345 SM00355 Zinc finger IPR015880 351 373 SM00355 Zinc finger ProfileScan IPR007087 183 210 PS50157 Zinc finger IPR007087 211 238 PS50157 Zinc finger IPR007087 239 266 PS50157 Zinc finger IPR007087 267 294 PS50157 Zinc finger IPR007087 295 322 PS50157 Zinc finger IPR007087 323 350 PS50157 Zinc finger IPR007087 351 376 PS50157 Zinc finger
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_612474 376 165280 100.0 100.0 XP_016868071 410 194510 47.6 86.2 XP_006716174 410 194510 47.6 86.2 NP_001265221 410 194510 47.6 86.2 XP_016868072 410 194510 47.6 86.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1053 Download

>KIEE1053  1128 bp
ATGGCAGCCCTAGGGGACATTCAGGAGTCCCCTTCTGTCCCGTCCCCTGTCAGTCTCTCA
TCACCGGGGACACCTGGAACCCAGCACCACGAGCCTCAGCTTCACCTCCATGGGCATCAA
CATGGCTCCCCTGGCTCCAGCCCTAAGGTGCTCTCCCAGCCGTCCGACCTGGATCTCCAA
GACGTAGAGGAAGTGGAGATCGGCAGAGACACCTTCTGGCCCGACTCCGAGCCCAAGCCG
GAGCAGGCTCCACGCTCTCCTGGCTCTCAGGCCCCTGACGAGGGGGCGGGCGGGGCGCTG
CGCAGCCTCCTGAGGAGCCTTCCCCGCAGGGCCCGGTGCAGCGCCGGCTTCGGGCCTGAA
TCCAGCGCGGAGCGGCCGGCGGGCCAGCCGCCTGGGGCCGTCCCTTGCGCCCAGCCGCGG
GGCGCCTGGCGCGTGACGCTCGTGCAGCAAGCAGCGGCTGGGCCCGAGGGTGCGCCCGAG
CGGGCTGCCGAGCTGGGAGTCAACTTCGGTCGGAGCCGGCAGGGCAGCGCGCGGGGGGCC
AAGCCGCACAGGTGCGAGGCCTGCGGCAAGAGTTTCAAGTATAACTCGCTGCTCCTGAAG
CACCAGCGCATCCACACGGGCGAGAAGCCCTACGCCTGCCACGAGTGCGGCAAGCGCTTC
CGCGGCTGGTCGGGCTTCATCCAGCACCACCGCATCCACACGGGCGAGAAGCCCTACGAG
TGCGGCCAGTGCGGCCGCGCCTTCAGCCACAGCTCGCACTTCACGCAGCACCTGCGCATC
CACAACGGCGAGAAGCCCTACAAGTGCGGCGAGTGCGGCCAGGCCTTCAGCCAGAGCTCC
AACCTGGTGCGCCACCAGCGGCTGCACACGGGTGAGAAGCCCTACGCCTGCAGCCAGTGC
GGCAAGGCCTTCATCTGGAGCTCCGTGCTCATCGAGCACCAGCGCATCCACACTGGCGAG
AAGCCCTACGAGTGCTCCGACTGCGGCAAAGCCTTCCGCGGCCGCTCGCACTTCTTCCGG
CACCTGCGGACCCACACGGGCGAGAAGCCCTTCGCGTGTGGCGCCTGCGGCAAGGCCTTC
GGCCAGAGCTCCCAGCTCATCCAGCACCAGCGGGTGCACTACCGCGAG

ORF Protein

Cloned ORF protein sequence for pFN21AE1053 Download

>KIEE1053  376 aa
MAALGDIQESPSVPSPVSLSSPGTPGTQHHEPQLHLHGHQHGSPGSSPKVLSQPSDLDLQ
DVEEVEIGRDTFWPDSEPKPEQAPRSPGSQAPDEGAGGALRSLLRSLPRRARCSAGFGPE
SSAERPAGQPPGAVPCAQPRGAWRVTLVQQAAAGPEGAPERAAELGVNFGRSRQGSARGA
KPHRCEACGKSFKYNSLLLKHQRIHTGEKPYACHECGKRFRGWSGFIQHHRIHTGEKPYE
CGQCGRAFSHSSHFTQHLRIHNGEKPYKCGECGQAFSQSSNLVRHQRLHTGEKPYACSQC
GKAFIWSSVLIEHQRIHTGEKPYECSDCGKAFRGRSHFFRHLRTHTGEKPFACGACGKAF
GQSSQLIQHQRVHYRE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1053 Download

>pFN21AE1053  5833 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAGCCCTAGGGGACATTCAGGAGTCCCCTTC
TGTCCCGTCCCCTGTCAGTCTCTCATCACCGGGGACACCTGGAACCCAGCACCACGAGCC
TCAGCTTCACCTCCATGGGCATCAACATGGCTCCCCTGGCTCCAGCCCTAAGGTGCTCTC
CCAGCCGTCCGACCTGGATCTCCAAGACGTAGAGGAAGTGGAGATCGGCAGAGACACCTT
CTGGCCCGACTCCGAGCCCAAGCCGGAGCAGGCTCCACGCTCTCCTGGCTCTCAGGCCCC
TGACGAGGGGGCGGGCGGGGCGCTGCGCAGCCTCCTGAGGAGCCTTCCCCGCAGGGCCCG
GTGCAGCGCCGGCTTCGGGCCTGAATCCAGCGCGGAGCGGCCGGCGGGCCAGCCGCCTGG
GGCCGTCCCTTGCGCCCAGCCGCGGGGCGCCTGGCGCGTGACGCTCGTGCAGCAAGCAGC
GGCTGGGCCCGAGGGTGCGCCCGAGCGGGCTGCCGAGCTGGGAGTCAACTTCGGTCGGAG
CCGGCAGGGCAGCGCGCGGGGGGCCAAGCCGCACAGGTGCGAGGCCTGCGGCAAGAGTTT
CAAGTATAACTCGCTGCTCCTGAAGCACCAGCGCATCCACACGGGCGAGAAGCCCTACGC
CTGCCACGAGTGCGGCAAGCGCTTCCGCGGCTGGTCGGGCTTCATCCAGCACCACCGCAT
CCACACGGGCGAGAAGCCCTACGAGTGCGGCCAGTGCGGCCGCGCCTTCAGCCACAGCTC
GCACTTCACGCAGCACCTGCGCATCCACAACGGCGAGAAGCCCTACAAGTGCGGCGAGTG
CGGCCAGGCCTTCAGCCAGAGCTCCAACCTGGTGCGCCACCAGCGGCTGCACACGGGTGA
GAAGCCCTACGCCTGCAGCCAGTGCGGCAAGGCCTTCATCTGGAGCTCCGTGCTCATCGA
GCACCAGCGCATCCACACTGGCGAGAAGCCCTACGAGTGCTCCGACTGCGGCAAAGCCTT
CCGCGGCCGCTCGCACTTCTTCCGGCACCTGCGGACCCACACGGGCGAGAAGCCCTTCGC
GTGTGGCGCCTGCGGCAAGGCCTTCGGCCAGAGCTCCCAGCTCATCCAGCACCAGCGGGT
GCACTACCGCGAGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT