################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI ApoI AscI AseI AsnI Asp718I AspEI AspI BalI BamHI BbrPI BbsI BciVI BclI BfrI BglII BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EarI EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI Ksp632I MamI MfeI MluI MluNI MroI MscI MseI MunI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SalI SapI SbfI ScaI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### Alw44I 1114 GTGCAC G^TGCAC AlwI 171 GGATC GGATC(4/5) Aor51HI 653 AGCGCT AGC^GCT ApaLI 1114 GTGCAC G^TGCAC Asp700I 1044 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 1028 GGACC G^G[AT]CC AvrII 9 CCTAGG C^CTAGG BbvCI 94 CCTCAGC CCTCAGC(-5/-2) BfaI 10 CTAG C^TAG BlnI 9 CCTAGG C^CTAGG BseRI 312 GAGGAG GAGGAG(10/8) BssHII 527 GCGCGC G^CGCGC BstXI 384 CCAGCCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 171 GGATCT [GA]^GATC[CT] Eco47III 653 AGCGCT AGC^GCT HgaI 435 GACGC GACGC(5/10) HincII 499 GTCAAC GT[CT]^[GA]AC HindII 499 GTCAAC GT[CT]^[GA]AC MaeI 10 CTAG C^TAG MaeII 182 ACGT A^CGT MaeIII 433 GTGAC ^GT[ACGT]AC MboII 1013 TCTTC GAAGA(8/7) MflI 171 GGATCT [GA]^GATC[CT] NcoI 108 CCATGG C^CATGG PstI 888 CTGCAG CTGCA^G SexAI 842 ACCTGGT A^CC[AT]GGT SfcI 888 CTGCAG C^T[GA][CT]AG SfiI 724 GGCCAGTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC TaqI 932 TCGA T^CGA TfiI 358 GAATC G^A[AT]TC Tsp45I 433 GTGAC ^GT[GC]AC TthHB8I 932 TCGA T^CGA XhoII 171 GGATCT [GA]^GATC[CT] XmnI 1044 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcyI 421 GGCGCC G[GA]^CG[CT]C 1060 GGCGCC AocI 142 CCTAAGG CC^T[ACGT]AGG 309 CCTGAGG ApaI 329 GGGCCC GGGCC^C 460 GGGCCC AvaI 463 CCCGAG C^[CT]CG[GA]G 475 CCCGAG AviII 299 TGCGCA TGC^GCA 773 TGCGCA BbeI 421 GGCGCC GGCGC^C 1060 GGCGCC BcgI 477 CGAGCGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1070 GCAAGGCCTTCG BcnI 64 CCGGG CC^[GC]GG 332 CCCGG BpmI 135 CTCCAG CTGGAG(16/14) 915 CTGGAG BsaHI 421 GGCGCC G[GA]^CG[CT]C 1060 GGCGCC BsmAI 206 GAGAC GTCTC(1/5) 53 GTCTC BsoBI 463 CCCGAG C^[CT]CG[GA]G 475 CCCGAG BspMI 770 ACCTGC ACCTGC(4/8) 1022 ACCTGC BstAPI 765 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 890 GCAGCCAGTGC Bsu36I 142 CCTAAGG CC^T[ACGT]AGG 309 CCTGAGG DpnII 172 GATC ^GATC 198 GATC DraII 269 AGGCCCC [GA]G^G[ACGT]CC[CT] 328 AGGGCCC Eco81I 142 CCTAAGG CC^T[ACGT]AGG 309 CCTGAGG EcoO109I 269 AGGCCCC [GA]G^G[ACGT]CC[CT] 328 AGGGCCC EcoT14I 9 CCTAGG C^C[AT][AT]GG 108 CCATGG FspI 299 TGCGCA TGC^GCA 773 TGCGCA Hin1I 421 GGCGCC G[GA]^CG[CT]C 1060 GGCGCC KasI 421 GGCGCC G^GCGCC 1060 GGCGCC MboI 172 GATC ^GATC 198 GATC NarI 421 GGCGCC GG^CGCC 1060 GGCGCC NciI 64 CCGGG CC^[GC]GG 332 CCCGG NdeII 172 GATC ^GATC 198 GATC NlaIII 109 CATG CATG^ 121 CATG NotI 731 GCGGCCGC GC^GGCCGC 998 GCGGCCGC Sau3AI 172 GATC ^GATC 198 GATC SfoI 421 GGCGCC GGC^GCC 1060 GGCGCC StyI 9 CCTAGG C^C[AT][AT]GG 108 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AluI AlwNI AspHI BanI BanII BbvI BglI BmyI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrBI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DsaI EaeI EagI EclXI Eco52I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI KspI MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NgoMIV NlaIV PleI SacI SacII Sau96I ScrFI SfaNI StuI TseI TspRI