(Linear) (Six-base) MAPSORT of: KIEE1053.seq Check: 1106 from: 1 to: 1128 KIEE1053.orf With 182 enzymes: SgfI * March 2, 2010 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 779 1031 1128 Size: 779 252 97 Fragments arranged by size: 779 252 97 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 616 726 810 1128 Size: 616 110 84 318 Fragments arranged by size: 616 318 110 84 AfeI AGC'GCT Cuts at: 0 655 1128 Size: 655 473 AleI CACnn'nnGTG Cuts at: 0 869 1128 Size: 869 259 AlwNI CAG_nnn'CTG Cuts at: 0 457 771 861 1128 Size: 457 314 90 267 Fragments arranged by size: 457 314 267 90 ApaI G_GGCC'C Cuts at: 0 333 464 1128 Size: 333 131 664 Fragments arranged by size: 664 333 131 ApaLI G'TGCA_C Cuts at: 0 1114 1128 Size: 1114 14 AvaI C'yCGr_G Cuts at: 0 463 475 1128 Size: 463 12 653 Fragments arranged by size: 653 463 12 AvrII C'CTAG_G Cuts at: 0 9 1128 Size: 9 1119 BanI G'GyrC_C Cuts at: 0 421 1019 1060 1128 Size: 421 598 41 68 Fragments arranged by size: 598 421 68 41 BanII G_rGCy'C Cuts at: 0 233 333 464 838 922 1090 1128 Size: 233 100 131 374 84 168 38 Fragments arranged by size: 374 233 168 131 100 84 38 BbeI G_GCGC'C Cuts at: 0 425 1064 1128 Size: 425 639 64 Fragments arranged by size: 639 425 64 BbvCI CC'TCA_GC Cuts at: 0 95 1128 Size: 95 1033 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 466 500 1059 1093 1128 Size: 466 34 559 34 35 Fragments arranged by size: 559 466 35 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 521 731 899 1128 Size: 521 210 168 229 Fragments arranged by size: 521 229 210 168 Bme1580I G_kGCm'C Cuts at: 0 333 464 1118 1128 Size: 333 131 654 10 Fragments arranged by size: 654 333 131 10 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 120 936 1128 Size: 120 816 192 Fragments arranged by size: 816 192 120 Bpu10I CC'TnA_GC Cuts at: 0 95 1128 Size: 95 1033 BsaHI Gr'CG_yC Cuts at: 0 422 1061 1128 Size: 422 639 67 Fragments arranged by size: 639 422 67 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 958 988 1128 Size: 958 30 140 Fragments arranged by size: 958 140 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 327 1128 Size: 327 801 BseYI C'CCAG_C Cuts at: 0 80 155 410 457 492 1091 1128 Size: 80 75 255 47 35 599 37 Fragments arranged by size: 599 255 80 75 47 37 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 357 463 847 1128 Size: 357 106 384 281 Fragments arranged by size: 384 357 281 106 BsiEI CG_ry'CG Cuts at: 0 376 510 735 1002 1128 Size: 376 134 225 267 126 Fragments arranged by size: 376 267 225 134 126 BsiHKAI G_wGCw'C Cuts at: 0 152 838 922 929 938 976 1090 1118 Size: 152 686 84 7 9 38 114 28 Cuts at: 1118 1128 Size: 10 Fragments arranged by size: 686 152 114 84 38 28 10 9 7 Bsp1286I G_dGCh'C Cuts at: 0 152 233 333 464 838 922 929 938 Size: 152 81 100 131 374 84 7 9 Cuts at: 938 976 1090 1118 1128 Size: 38 114 28 10 Fragments arranged by size: 374 152 131 114 100 84 81 38 28 10 9 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 779 1031 1128 Size: 779 252 97 Fragments arranged by size: 779 252 97 BsrBI CCG'CTC Cuts at: 0 372 480 1004 1128 Size: 372 108 524 124 Fragments arranged by size: 524 372 124 108 BsrFI r'CCGG_y Cuts at: 0 343 375 515 1128 Size: 343 32 140 613 Fragments arranged by size: 613 343 140 32 BssHII G'CGCG_C Cuts at: 0 527 1128 Size: 527 601 BssSI C'ACGA_G Cuts at: 0 88 439 640 1128 Size: 88 351 201 488 Fragments arranged by size: 488 351 201 88 BstAPI GCAn_nnn'nTGC Cuts at: 0 771 896 1128 Size: 771 125 232 Fragments arranged by size: 771 232 125 BstXI CCAn_nnnn'nTGG Cuts at: 0 391 1128 Size: 391 737 BstYI r'GATC_y Cuts at: 0 171 1128 Size: 171 957 Bsu36I CC'TnA_GG Cuts at: 0 143 310 1128 Size: 143 167 818 Fragments arranged by size: 818 167 143 BtgI C'CryG_G Cuts at: 0 108 415 660 996 1128 Size: 108 307 245 336 132 Fragments arranged by size: 336 307 245 132 108 EaeI y'GGCC_r Cuts at: 0 373 723 732 816 999 1080 1128 Size: 373 350 9 84 183 81 48 Fragments arranged by size: 373 350 183 84 81 48 9 EagI C'GGCC_G Cuts at: 0 373 732 999 1128 Size: 373 359 267 129 Fragments arranged by size: 373 359 267 129 EcoICRI GAG'CTC Cuts at: 0 836 920 1088 1128 Size: 836 84 168 40 Fragments arranged by size: 836 168 84 40 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 120 616 726 810 936 1128 Size: 120 496 110 84 126 192 Fragments arranged by size: 496 192 126 120 110 84 EcoO109I rG'GnC_Cy Cuts at: 0 270 329 1128 Size: 270 59 799 Fragments arranged by size: 799 270 59 FspI TGC'GCA Cuts at: 0 301 775 1128 Size: 301 474 353 Fragments arranged by size: 474 353 301 HaeII r_GCGC'y Cuts at: 0 298 344 425 657 1064 1128 Size: 298 46 81 232 407 64 Fragments arranged by size: 407 298 232 81 64 46 HincII GTy'rAC Cuts at: 0 501 1128 Size: 501 627 KasI G'GCGC_C Cuts at: 0 421 1060 1128 Size: 421 639 68 Fragments arranged by size: 639 421 68 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 188 490 863 1001 1128 Size: 188 302 373 138 127 Fragments arranged by size: 373 302 188 138 127 MslI CAynn'nnrTG Cuts at: 0 107 119 869 1128 Size: 107 12 750 259 Fragments arranged by size: 750 259 107 12 MspA1I CmG'CkG Cuts at: 0 417 454 662 858 998 1110 1128 Size: 417 37 208 196 140 112 18 Fragments arranged by size: 417 208 196 140 112 37 18 NaeI GCC'GGC Cuts at: 0 345 377 517 1128 Size: 345 32 140 611 Fragments arranged by size: 611 345 140 32 NarI GG'CG_CC Cuts at: 0 422 1061 1128 Size: 422 639 67 Fragments arranged by size: 639 422 67 NcoI C'CATG_G Cuts at: 0 108 1128 Size: 108 1020 NgoMIV G'CCGG_C Cuts at: 0 343 375 515 1128 Size: 343 32 140 613 Fragments arranged by size: 613 343 140 32 NotI GC'GGCC_GC Cuts at: 0 732 999 1128 Size: 732 267 129 Fragments arranged by size: 732 267 129 PspOMI G'GGCC_C Cuts at: 0 329 460 1128 Size: 329 131 668 Fragments arranged by size: 668 329 131 PstI C_TGCA'G Cuts at: 0 892 1128 Size: 892 236 SacI G_AGCT'C Cuts at: 0 838 922 1090 1128 Size: 838 84 168 38 Fragments arranged by size: 838 168 84 38 SacII CC_GC'GG Cuts at: 0 418 663 999 1128 Size: 418 245 336 129 Fragments arranged by size: 418 336 245 129 SexAI A'CCwGG_T Cuts at: 0 842 1128 Size: 842 286 SfcI C'TryA_G Cuts at: 0 888 1128 Size: 888 240 SfiI GGCCn_nnn'nGGCC Cuts at: 0 731 1128 Size: 731 397 SfoI GGC'GCC Cuts at: 0 423 1062 1128 Size: 423 639 66 Fragments arranged by size: 639 423 66 StuI AGG'CCT Cuts at: 0 559 823 907 1075 1128 Size: 559 264 84 168 53 Fragments arranged by size: 559 264 168 84 53 StyI C'CwwG_G Cuts at: 0 9 108 1128 Size: 9 99 1020 Fragments arranged by size: 1020 99 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 496 1128 Size: 496 632 XmnI GAAnn'nnTTC Cuts at: 0 1048 1128 Size: 1048 80 Enzymes that do cut: AarI AcuI AfeI AleI AlwNI ApaI ApaLI AvaI AvrII BanI BanII BbeI BbvCI BcgI BglI Bme1580I BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI EaeI EagI EcoICRI Eco57MI EcoO109I FspI HaeII HincII KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NotI PspOMI PstI SacI SacII SexAI SfcI SfiI SfoI StuI StyI TaqII XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI BaeI BamHI BbsI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI EciI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I HindIII HpaI KpnI MfeI MluI MscI NdeI NheI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI