S1PR3 ORF clone FHC01830

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Halotag ORF Clone FHC01830

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Clone info.

Species

human

Clone Name

pFN21AB5664 Sequence (with vector) Restriction Map

Product ID

FHC01830

Flexi Clone PID

FXC01830

Original Clone PID

ORK01830

ORF Sequence

DNA sequence (1134 bp)
Protein sequence (378 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385149]

Gene

KIBB5664

Symbol

S1PR3

Alias : C9orf108, C9orf47, EDG-3, EDG3, LPB3, S1P3, bA791O21.3

Description

sphingosine-1-phosphate receptor 3

Original Clone ID

ej00993

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1134 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 378 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS6680.1 5.9e-89 100.0 S1PR3 CCDS777.1 1.4e-42 55.8 S1PR1 CCDS12229.1 5.6e-33 47.9 S1PR2 CCDS12105.1 3.6e-27 42.8 S1PR4 CCDS6777.1 8.3e-24 38.4 LPAR1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR004061 13 25 PR01523 Sphingosine 1-phosphate receptor IPR004062 24 40 PR01524 EDG-3 sphingosine 1-phosphate receptor IPR000276 41 65 PR00237 GPCR IPR000276 74 95 PR00237 GPCR IPR004061 92 101 PR01523 Sphingosine 1-phosphate receptor IPR004061 112 123 PR01523 Sphingosine 1-phosphate receptor IPR000276 118 140 PR00237 GPCR IPR004062 139 155 PR01524 EDG-3 sphingosine 1-phosphate receptor IPR000276 153 174 PR00237 GPCR IPR004061 194 204 PR01523 Sphingosine 1-phosphate receptor IPR000276 196 219 PR00237 GPCR IPR004061 215 224 PR01523 Sphingosine 1-phosphate receptor IPR004062 223 233 PR01524 EDG-3 sphingosine 1-phosphate receptor IPR000276 241 265 PR00237 GPCR IPR004061 272 283 PR01523 Sphingosine 1-phosphate receptor IPR000276 280 306 PR00237 GPCR IPR004061 301 311 PR01523 Sphingosine 1-phosphate receptor IPR004062 314 323 PR01524 EDG-3 sphingosine 1-phosphate receptor IPR004062 326 340 PR01524 EDG-3 sphingosine 1-phosphate receptor HMMPfam IPR000276 56 298 PF00001 GPCR ProfileScan IPR017452 56 298 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 41 LTTVLFLVICSFIVLENLMVLIA 63 PRIMARY 23 2 74 MYFFIGNLALCDLLAGIAYKVNI 96 SECONDARY 23 3 117 SMFVALGASTCSLLAIAIERHLT 139 SECONDARY 23 4 154 VFLLIGMCWLIAFTLGALPILGW 176 PRIMARY 23 5 200 CISIFTAILVTIVILYARIYFLV 222 PRIMARY 23 6 242 LRTVVIVVSVFIACWSPLFILFL 264 PRIMARY 23 7 277 LFKAQWFIVLAVLNSAMNPVIYT 299 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005217 378 601965 100.0 100.0 NP_001307659 382 601974 55.8 90.5 NP_001391 382 601974 55.8 90.5 NP_004221 353 605111,610419,610419 47.9 87.8 NP_003766 384 603751 42.8 88.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB5664 Download

>KIBB5664  1134 bp
ATGGCAACTGCCCTCCCGCCGCGTCTCCAGCCGGTGCGGGGGAACGAGACCCTGCGGGAG
CATTACCAGTACGTGGGGAAGTTGGCGGGCAGGCTGAAGGAGGCCTCCGAGGGCAGCACG
CTCACCACCGTGCTCTTCTTGGTCATCTGCAGCTTCATCGTCTTGGAGAACCTGATGGTT
TTGATTGCCATCTGGAAAAACAATAAATTTCACAACCGCATGTACTTTTTCATTGGCAAC
CTGGCTCTCTGCGACCTGCTGGCCGGCATCGCTTACAAGGTCAACATTCTGATGTCTGGC
AAGAAGACGTTCAGCCTGTCTCCCACGGTCTGGTTCCTCAGGGAGGGCAGTATGTTCGTG
GCCCTTGGGGCGTCCACCTGCAGCTTACTGGCCATCGCCATCGAGCGGCACTTGACAATG
ATCAAAATGAGGCCTTACGACGCCAACAAGAGGCACCGCGTCTTCCTCCTGATCGGGATG
TGCTGGCTCATTGCCTTCACGCTGGGCGCCCTGCCCATTCTGGGCTGGAACTGCCTGCAC
AATCTCCCTGACTGCTCTACCATCCTGCCCCTCTACTCCAAGAAGTACATTGCCTTCTGC
ATCAGCATCTTCACGGCCATCCTGGTGACCATCGTGATCCTCTACGCACGCATCTACTTC
CTGGTGAAGTCCAGCAGCCGTAAGGTGGCCAACCACAACAACTCGGAGCGGTCCATGGCA
CTGCTGCGGACCGTGGTGATTGTGGTGAGCGTGTTCATCGCCTGCTGGTCCCCACTCTTC
ATCCTCTTCCTCATTGATGTGGCCTGCAGGGTGCAGGCGTGCCCCATCCTCTTCAAGGCT
CAGTGGTTCATCGTGTTGGCTGTGCTCAACTCCGCCATGAACCCGGTCATCTACACGCTG
GCCAGCAAGGAGATGCGGCGGGCCTTCTTCCGTCTGGTCTGCAACTGCCTGGTCAGGGGA
CGGGGGGCCCGCGCCTCACCCATCCAGCCTGCGCTCGACCCAAGCAGAAGTAAATCAAGC
AGCAGCAACAATAGCAGCCACTCTCCGAAGGTCAAGGAAGACCTGCCCCACACAGCCCCC
TCATCCTGCATCATGGACAAGAACGCAGCACTTCAGAATGGGATCTTCTGCAAC

ORF Protein

Cloned ORF protein sequence for pFN21AB5664 Download

>KIBB5664  378 aa
MATALPPRLQPVRGNETLREHYQYVGKLAGRLKEASEGSTLTTVLFLVICSFIVLENLMV
LIAIWKNNKFHNRMYFFIGNLALCDLLAGIAYKVNILMSGKKTFSLSPTVWFLREGSMFV
ALGASTCSLLAIAIERHLTMIKMRPYDANKRHRVFLLIGMCWLIAFTLGALPILGWNCLH
NLPDCSTILPLYSKKYIAFCISIFTAILVTIVILYARIYFLVKSSSRKVANHNNSERSMA
LLRTVVIVVSVFIACWSPLFILFLIDVACRVQACPILFKAQWFIVLAVLNSAMNPVIYTL
ASKEMRRAFFRLVCNCLVRGRGARASPIQPALDPSRSKSSSSNNSSHSPKVKEDLPHTAP
SSCIMDKNAALQNGIFCN

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB5664 Download

>pFN21AB5664  5839 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAACTGCCCTCCCGCCGCGTCTCCAGCCGGT
GCGGGGGAACGAGACCCTGCGGGAGCATTACCAGTACGTGGGGAAGTTGGCGGGCAGGCT
GAAGGAGGCCTCCGAGGGCAGCACGCTCACCACCGTGCTCTTCTTGGTCATCTGCAGCTT
CATCGTCTTGGAGAACCTGATGGTTTTGATTGCCATCTGGAAAAACAATAAATTTCACAA
CCGCATGTACTTTTTCATTGGCAACCTGGCTCTCTGCGACCTGCTGGCCGGCATCGCTTA
CAAGGTCAACATTCTGATGTCTGGCAAGAAGACGTTCAGCCTGTCTCCCACGGTCTGGTT
CCTCAGGGAGGGCAGTATGTTCGTGGCCCTTGGGGCGTCCACCTGCAGCTTACTGGCCAT
CGCCATCGAGCGGCACTTGACAATGATCAAAATGAGGCCTTACGACGCCAACAAGAGGCA
CCGCGTCTTCCTCCTGATCGGGATGTGCTGGCTCATTGCCTTCACGCTGGGCGCCCTGCC
CATTCTGGGCTGGAACTGCCTGCACAATCTCCCTGACTGCTCTACCATCCTGCCCCTCTA
CTCCAAGAAGTACATTGCCTTCTGCATCAGCATCTTCACGGCCATCCTGGTGACCATCGT
GATCCTCTACGCACGCATCTACTTCCTGGTGAAGTCCAGCAGCCGTAAGGTGGCCAACCA
CAACAACTCGGAGCGGTCCATGGCACTGCTGCGGACCGTGGTGATTGTGGTGAGCGTGTT
CATCGCCTGCTGGTCCCCACTCTTCATCCTCTTCCTCATTGATGTGGCCTGCAGGGTGCA
GGCGTGCCCCATCCTCTTCAAGGCTCAGTGGTTCATCGTGTTGGCTGTGCTCAACTCCGC
CATGAACCCGGTCATCTACACGCTGGCCAGCAAGGAGATGCGGCGGGCCTTCTTCCGTCT
GGTCTGCAACTGCCTGGTCAGGGGACGGGGGGCCCGCGCCTCACCCATCCAGCCTGCGCT
CGACCCAAGCAGAAGTAAATCAAGCAGCAGCAACAATAGCAGCCACTCTCCGAAGGTCAA
GGAAGACCTGCCCCACACAGCCCCCTCATCCTGCATCATGGACAAGAACGCAGCACTTCA
GAATGGGATCTTCTGCAACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT