(Circular) (Six-base) MAPSORT of: pFN21AB5664.seq Check: 6318 from: 1 to: 5839 pFN21AB5664 5839 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2390 2390 Size: 5839 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3649 278 Size: 53 83 186 936 2113 2468 Fragments arranged by size: 2468 2113 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3182 1937 Size: 1245 4594 Acc65I G'GTAC_C Cuts at: 3160 3160 Size: 5839 AclI AA'CG_TT Cuts at: 1563 3138 3966 4339 1563 Size: 1575 828 373 3063 Fragments arranged by size: 3063 1575 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2120 3101 3902 5286 5554 1632 Size: 488 981 801 1384 268 1917 Fragments arranged by size: 1917 1384 981 801 488 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5642 AflIII A'CryG_T Cuts at: 1203 4738 5715 1203 Size: 3535 977 1327 Fragments arranged by size: 3535 1327 977 AgeI A'CCGG_T Cuts at: 4670 4670 Size: 5839 AhdI GACnn_n'nnGTC Cuts at: 4568 4568 Size: 5839 AleI CACnn'nnGTG Cuts at: 1166 2132 1166 Size: 966 4873 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2933 1184 Size: 1749 4090 AlwNI CAG_nnn'CTG Cuts at: 3582 5154 3582 Size: 1572 4267 ApaI G_GGCC'C Cuts at: 2974 2974 Size: 5839 ApaLI G'TGCA_C Cuts at: 3896 5052 3896 Size: 1156 4683 ApoI r'AATT_y Cuts at: 1493 2210 3148 3374 3526 5635 1493 Size: 717 938 226 152 2109 1697 Fragments arranged by size: 2109 1697 938 717 226 152 AseI AT'TA_AT Cuts at: 160 1032 4393 160 Size: 872 3361 1606 Fragments arranged by size: 3361 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 AvaI C'yCGr_G Cuts at: 1870 1943 3164 1870 Size: 73 1221 4545 Fragments arranged by size: 4545 1221 73 BamHI G'GATC_C Cuts at: 3169 3536 3169 Size: 367 5472 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2457 2510 3160 4615 618 Size: 325 217 627 670 53 650 1455 1842 Fragments arranged by size: 1842 1455 670 650 627 325 217 53 BanII G_rGCy'C Cuts at: 1391 2974 3158 1391 Size: 1583 184 4072 Fragments arranged by size: 4072 1583 184 BbeI G_GCGC'C Cuts at: 2514 2514 Size: 5839 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2315 2458 3069 928 Size: 971 416 143 611 3698 Fragments arranged by size: 3698 971 611 416 143 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2143 2177 2341 2375 4030 4064 1903 Size: 34 206 34 164 34 1655 34 3678 Fragments arranged by size: 3678 1655 206 164 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3731 4941 3731 Size: 1210 4629 BclI T'GATC_A Cuts at: 1548 2424 1548 Size: 876 4963 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2372 4450 136 Size: 107 122 71 631 605 700 2078 1525 Fragments arranged by size: 2078 1525 700 631 605 122 107 71 BglII A'GATC_T Cuts at: 5834 5834 Size: 5839 BlpI GC'TnA_GC Cuts at: 3256 3256 Size: 5839 Bme1580I G_kGCm'C Cuts at: 1790 2828 2974 3900 5056 1790 Size: 1038 146 926 1156 2573 Fragments arranged by size: 2573 1156 1038 926 146 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5839 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3570 4528 442 Size: 416 53 83 399 24 2153 958 1753 Fragments arranged by size: 2153 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5839 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2015 4499 1239 Size: 776 2484 2579 Fragments arranged by size: 2579 2484 776 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3964 4829 5127 3964 Size: 865 298 4676 Fragments arranged by size: 4676 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2045 4502 882 Size: 617 546 2457 2219 Fragments arranged by size: 2457 2219 617 546 BsaAI yAC'GTr Cuts at: 493 1204 2077 3630 493 Size: 711 873 1553 2702 Fragments arranged by size: 2702 1553 873 711 BsaBI GATnn'nnATC Cuts at: 2192 5833 2192 Size: 3641 2198 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2375 2445 2511 3646 Size: 53 83 186 936 842 70 66 1135 Cuts at: 3646 4028 4663 275 Size: 382 635 1451 Fragments arranged by size: 1451 1135 936 842 635 382 186 83 70 66 53 BsaWI w'CCGG_w Cuts at: 4272 4670 4944 5091 5502 4272 Size: 398 274 147 411 4609 Fragments arranged by size: 4609 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5614 Fragments arranged by size: 5614 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5839 BseYI C'CCAG_C Cuts at: 2506 5042 5538 2506 Size: 2536 496 2807 Fragments arranged by size: 2807 2536 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2525 2837 1163 Size: 573 789 312 4165 Fragments arranged by size: 4165 789 573 312 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3289 4050 5078 1540 Size: 275 186 1288 761 1028 2301 Fragments arranged by size: 2301 1288 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2139 2871 3900 3985 5056 2139 Size: 732 1029 85 1071 2922 Fragments arranged by size: 2922 1071 1029 732 85 BsmI GAATG_Cn' Cuts at: 3350 3443 3350 Size: 93 5746 BsmBI CGTCTCn'nnnn_ Cuts at: 2033 2033 Size: 5839 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2139 2828 2871 2974 3158 3900 3985 Size: 399 349 689 43 103 184 742 85 Cuts at: 3985 5056 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 689 399 349 184 103 85 43 BspEI T'CCGG_A Cuts at: 5502 5502 Size: 5839 BspHI T'CATG_A Cuts at: 3728 5458 3728 Size: 1730 4109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2268 2390 3075 3194 844 Size: 1424 122 685 119 3489 Fragments arranged by size: 3489 1424 685 122 119 BsrBI CCG'CTC Cuts at: 1394 1823 2410 2713 3726 1394 Size: 429 587 303 1013 3507 Fragments arranged by size: 3507 1013 587 429 303 BsrDI GCAATG_nn' Cuts at: 65 1248 2493 2592 4334 4508 65 Size: 1183 1245 99 1742 174 1396 Fragments arranged by size: 1742 1396 1245 1183 174 99 BsrFI r'CCGG_y Cuts at: 1700 1810 2035 2267 4483 4670 1700 Size: 110 225 232 2216 187 2869 Fragments arranged by size: 2869 2216 232 225 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5839 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5839 BssSI C'ACGA_G Cuts at: 3899 4911 3899 Size: 1012 4827 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5839 BstBI TT'CG_AA Cuts at: 4643 4643 Size: 5839 BstEII G'GTnAC_C Cuts at: 1184 2629 1184 Size: 1445 4394 BstYI r'GATC_y Cuts at: 1972 3126 3169 3536 3921 3938 5390 5476 5488 Size: 1154 43 367 385 17 1452 86 12 Cuts at: 5488 5834 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1154 385 367 346 86 43 17 12 Bsu36I CC'TnA_GG Cuts at: 2342 2342 Size: 5839 BtgI C'CryG_G Cuts at: 513 1065 2004 2328 2718 2736 513 Size: 552 939 324 390 18 3616 Fragments arranged by size: 3616 939 552 390 324 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2257 2383 2746 487 Size: 683 56 1031 126 363 3580 Fragments arranged by size: 3580 1031 683 363 126 56 BtsI GCAGTG_nn' Cuts at: 2723 3355 4149 4169 2723 Size: 632 794 20 4393 Fragments arranged by size: 4393 794 632 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5839 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5804 DraI TTT'AAA Cuts at: 3143 3143 Size: 5839 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4846 809 Size: 507 3530 1802 Fragments arranged by size: 3530 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2265 2394 2619 Size: 54 1425 50 275 18 435 129 225 Cuts at: 2619 2691 2904 3286 4175 5804 8 Size: 72 213 382 889 1629 43 Fragments arranged by size: 1629 1425 889 435 382 275 225 213 129 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3286 1537 Size: 275 1474 4090 Fragments arranged by size: 4090 1474 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2144 2786 2795 2840 3769 1353 Size: 791 642 9 45 929 3423 Fragments arranged by size: 3423 929 791 642 45 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2866 4426 4800 4946 387 Size: 1064 138 1277 1560 374 146 1280 Fragments arranged by size: 1560 1280 1277 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2015 2120 3101 3902 4499 5286 5554 Size: 393 383 105 981 801 597 787 268 Cuts at: 5554 1239 Size: 1524 Fragments arranged by size: 1524 981 801 787 597 393 383 268 105 EcoNI CCTnn'n_nnAGG Cuts at: 2345 2837 2345 Size: 492 5347 EcoO109I rG'GnC_Cy Cuts at: 2970 2970 Size: 5839 EcoRI G'AATT_C Cuts at: 3148 3526 3148 Size: 378 5461 FspI TGC'GCA Cuts at: 4345 4345 Size: 5839 HaeII r_GCGC'y Cuts at: 1678 2514 4986 1678 Size: 836 2472 2531 Fragments arranged by size: 2531 2472 836 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4480 4512 4554 4586 1373 Size: 32 135 32 2908 32 42 32 2626 Fragments arranged by size: 2908 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2287 3183 3429 669 Size: 988 281 349 896 246 3079 Fragments arranged by size: 3079 988 896 349 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5839 HpaI GTT'AAC Cuts at: 3429 3429 Size: 5839 KasI G'GCGC_C Cuts at: 2510 2510 Size: 5839 KpnI G_GTAC'C Cuts at: 3164 3164 Size: 5839 MfeI C'AATT_G Cuts at: 3438 3438 Size: 5839 MluI A'CGCG_T Cuts at: 5715 5715 Size: 5839 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4953 5137 1305 Size: 3648 184 2007 Fragments arranged by size: 3648 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2396 2693 2906 10 Size: 54 1425 343 564 297 213 2943 Fragments arranged by size: 2943 1425 564 343 297 213 54 MslI CAynn'nnrTG Cuts at: 518 1166 2132 2294 2627 2801 3797 4156 4315 Size: 648 966 162 333 174 996 359 159 Cuts at: 4315 518 Size: 2042 Fragments arranged by size: 2042 996 966 648 359 333 174 162 159 MspA1I CmG'CkG Cuts at: 1606 3255 3932 5080 5325 1606 Size: 1649 677 1148 245 2120 Fragments arranged by size: 2120 1649 1148 677 245 NaeI GCC'GGC Cuts at: 2269 2269 Size: 5839 NarI GG'CG_CC Cuts at: 2511 2511 Size: 5839 NcoI C'CATG_G Cuts at: 513 1065 2004 2718 513 Size: 552 939 714 3634 Fragments arranged by size: 3634 939 714 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5839 NgoMIV G'CCGG_C Cuts at: 2267 2267 Size: 5839 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5839 NotI GC'GGCC_GC Cuts at: 3286 3286 Size: 5839 NspI r_CATG'y Cuts at: 1132 1229 2227 3197 4742 1132 Size: 97 998 970 1545 2229 Fragments arranged by size: 2229 1545 998 970 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5839 PciI A'CATG_T Cuts at: 4738 4738 Size: 5839 PflMI CCAn_nnn'nTGG Cuts at: 1108 2077 2334 1108 Size: 969 257 4613 Fragments arranged by size: 4613 969 257 PmeI GTTT'AAAC Cuts at: 3143 3143 Size: 5839 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3908 3940 5421 5453 3908 Size: 32 1481 32 4294 Fragments arranged by size: 4294 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5839 PsiI TTA'TAA Cuts at: 3409 3409 Size: 5839 PspOMI G'GGCC_C Cuts at: 2970 2970 Size: 5839 PstI C_TGCA'G Cuts at: 830 2156 2387 2813 3191 830 Size: 1326 231 426 378 3478 Fragments arranged by size: 3478 1326 426 378 231 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5839 RsrII CG'GwC_CG Cuts at: 2733 2733 Size: 5839 SalI G'TCGA_C Cuts at: 1936 3181 1936 Size: 1245 4594 SapI GCTCTTCn'nnn_ Cuts at: 2144 2144 Size: 5839 SbfI CC_TGCA'GG Cuts at: 2813 3191 2813 Size: 378 5461 ScaI AGT'ACT Cuts at: 4087 5799 4087 Size: 1712 4127 SfcI C'TryA_G Cuts at: 826 1045 2152 2383 2809 3187 4322 5003 5194 Size: 219 1107 231 426 378 1135 681 191 Cuts at: 5194 826 Size: 1471 Fragments arranged by size: 1471 1135 1107 681 426 378 231 219 191 SfoI GGC'GCC Cuts at: 2512 2512 Size: 5839 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5839 SmaI CCC'GGG Cuts at: 1872 3166 1872 Size: 1294 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3943 4844 5106 820 Size: 197 926 2000 901 262 1553 Fragments arranged by size: 2000 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3630 493 Size: 3137 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5839 SphI G_CATG'C Cuts at: 1132 3197 1132 Size: 2065 3774 SspI AAT'ATT Cuts at: 5 52 3763 5 Size: 47 3711 2081 Fragments arranged by size: 3711 2081 47 StuI AGG'CCT Cuts at: 1517 2108 2437 1517 Size: 591 329 4919 Fragments arranged by size: 4919 591 329 StyI C'CwwG_G Cuts at: 513 1065 2004 2368 2718 3278 513 Size: 552 939 364 350 560 3074 Fragments arranged by size: 3074 939 560 552 364 350 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2998 3866 3883 4036 4221 4666 325 Size: 1255 1418 868 17 153 185 445 1498 Fragments arranged by size: 1498 1418 1255 868 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2226 2589 4085 Size: 274 80 33 51 1443 249 363 1496 Cuts at: 4085 5797 96 Size: 1712 138 Fragments arranged by size: 1712 1496 1443 363 274 249 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2769 2801 5799 5831 2769 Size: 32 2998 32 2777 Fragments arranged by size: 2998 2777 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5839 XbaI T'CTAG_A Cuts at: 3175 3175 Size: 5839 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5839 XmaI C'CCGG_G Cuts at: 1870 3164 1870 Size: 1294 4545 XmnI GAAnn'nnTTC Cuts at: 2312 3968 2312 Size: 1656 4183 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3647 276 Size: 53 83 186 936 2113 2468 Fragments arranged by size: 2468 2113 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI RsrII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI