Gene/Protein Characteristic Table for
FHC01507
Species
human
Product ID
FHC01507
Linker Type
Cloning Site
SgfI-PmeI
Gene
KIBB5324
Description
cholinergic receptor, muscarinic 1
Original Clone ID
bm03576
Characterization of the cloned ORF
N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand
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Length: 1380 bp
Restriction map A
Restriction map B
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Length: 460 aa
Result of homology search against nr database (
FASTA output,
Multiple alignment)
Entry Exp ID% Protein Source P11229 3.2e-171 100.0 Muscarinic acet... Homo sapiens AAP36136 3.2e-171 100.0 cholinergic rec... synthetic construct CAA33334 5.5e-171 99.7 unnamed protein... Homo sapiens P56489 1.2e-170 99.5 Muscarinic acet... Macaca mulatta ABM81651 1.8e-170 100.0 cholinergic rec... synthetic construct
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
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Result of InterProScan
Search method interpro_ID From To Entry Definition FPrintScan IPR002228 6 22 PR00538 Muscarinic acetylcholine receptor M1 IPR000276 27 51 PR00237 Rhodopsin-like GPCR superfamily IPR000995 27 39 PR00243 Muscarinic acetylcholine receptor IPR000995 48 56 PR00243 Muscarinic acetylcholine receptor IPR000276 60 81 PR00237 Rhodopsin-like GPCR superfamily IPR000995 75 85 PR00243 Muscarinic acetylcholine receptor IPR000276 105 127 PR00237 Rhodopsin-like GPCR superfamily IPR000995 106 115 PR00243 Muscarinic acetylcholine receptor IPR000995 125 139 PR00243 Muscarinic acetylcholine receptor IPR000276 141 162 PR00237 Rhodopsin-like GPCR superfamily IPR000995 162 172 PR00243 Muscarinic acetylcholine receptor IPR000995 182 193 PR00243 Muscarinic acetylcholine receptor IPR000276 189 212 PR00237 Rhodopsin-like GPCR superfamily IPR002228 258 277 PR00538 Muscarinic acetylcholine receptor M1 IPR002228 281 299 PR00538 Muscarinic acetylcholine receptor M1 IPR002228 303 320 PR00538 Muscarinic acetylcholine receptor M1 IPR002228 333 349 PR00538 Muscarinic acetylcholine receptor M1 IPR000276 363 387 PR00237 Rhodopsin-like GPCR superfamily IPR000995 390 408 PR00243 Muscarinic acetylcholine receptor IPR000276 400 426 PR00237 Rhodopsin-like GPCR superfamily IPR000995 421 435 PR00243 Muscarinic acetylcholine receptor IPR002228 441 458 PR00538 Muscarinic acetylcholine receptor M1 HMMPfam IPR000276 42 418 PF00001 Rhodopsin-like GPCR superfamily ProfileScan IPR000276 42 418 PS50262 Rhodopsin-like GPCR superfamily ScanRegExp IPR000276 111 127 PS00237 Rhodopsin-like GPCR superfamily
Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length SOSUI2 1 23 WQVAFIGITTGLLSLATVTGNLL 45 SECONDARY 23 2 58 TVNNYFLLSLACADLIIGTFSMN 80 SECONDARY 23 3 83 TTYLLMGHWALGTLACDLWLALD 105 SECONDARY 23 4 148 LAWLVSFVLWAPAILFWQYLVGE 170 PRIMARY 23 5 185 QPIITFGTAMAAFYLPVTVMCTL 207 SECONDARY 23 6 366 TLSAILLAFILTWTPYNIMVLVS 388 PRIMARY 23 7 397 ETLWELGYWLCYVNSTINPMCYA 419 SECONDARY 23
Result of homology search against disease genes in OMIM database
H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_000729 460 118510 100.0 100.0 NP_000731 590 118494 70.3 94.6 NP_036257 532 118496 69.3 92.8 NP_000732 479 118495 72.7 90.0 NP_001006631 466 118493 72.2 95.0 Inquiries or Suggestions ?
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