(Circular) (Six-base) MAPSORT of: pFN21AB5324.seq Check: 6281 from: 1 to: 6085 pFN21AB5324 6085 bp With 182 enzymes: SgfI * February 27, 2009 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2032 2032 Size: 6085 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3895 278 Size: 53 83 186 936 2359 2468 Fragments arranged by size: 2468 2359 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3428 1937 Size: 1491 4594 Acc65I G'GTAC_C Cuts at: 2228 3406 2228 Size: 1178 4907 AclI AA'CG_TT Cuts at: 1563 4212 4585 1563 Size: 2649 373 3063 Fragments arranged by size: 3063 2649 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 1998 2663 4148 5532 5800 1632 Size: 366 665 1485 1384 268 1917 Fragments arranged by size: 1917 1485 1384 665 366 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5888 AflIII A'CryG_T Cuts at: 1203 4984 5961 1203 Size: 3781 977 1327 Fragments arranged by size: 3781 1327 977 AgeI A'CCGG_T Cuts at: 4916 4916 Size: 6085 AhdI GACnn_n'nnGTC Cuts at: 4814 4814 Size: 6085 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6085 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6085 AlwNI CAG_nnn'CTG Cuts at: 2503 2747 3828 5400 2503 Size: 244 1081 1572 3188 Fragments arranged by size: 3188 1572 1081 244 ApaI G_GGCC'C Cuts at: 2479 2800 2479 Size: 321 5764 ApaLI G'TGCA_C Cuts at: 2617 3359 4142 5298 2617 Size: 742 783 1156 3404 Fragments arranged by size: 3404 1156 783 742 ApoI r'AATT_y Cuts at: 1493 3394 3620 3772 5881 1493 Size: 1901 226 152 2109 1697 Fragments arranged by size: 2109 1901 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4639 160 Size: 872 3607 1606 Fragments arranged by size: 3607 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 AvaI C'yCGr_G Cuts at: 1870 1943 2930 3191 3410 1870 Size: 73 987 261 219 4545 Fragments arranged by size: 4545 987 261 219 73 BamHI G'GATC_C Cuts at: 3415 3782 3415 Size: 367 5718 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2053 2228 3406 4861 618 Size: 325 217 627 266 175 1178 1455 1842 Fragments arranged by size: 1842 1455 1178 627 325 266 217 175 BanII G_rGCy'C Cuts at: 1391 2172 2281 2479 2687 2756 2800 2969 3404 Size: 781 109 198 208 69 44 169 435 Cuts at: 3404 1391 Size: 4072 Fragments arranged by size: 4072 781 435 208 198 169 109 69 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3068 928 Size: 971 1169 3945 Fragments arranged by size: 3945 1169 971 BbvCI CC'TCA_GC Cuts at: 2748 2748 Size: 6085 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4276 4310 1903 Size: 34 2339 34 3678 Fragments arranged by size: 3678 2339 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3977 5187 3977 Size: 1210 4875 BclI T'GATC_A Cuts at: 1548 2907 1548 Size: 1359 4726 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4696 136 Size: 107 122 71 631 605 3024 1525 Fragments arranged by size: 3024 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6080 6080 Size: 6085 BlpI GC'TnA_GC Cuts at: 2198 3502 2198 Size: 1304 4781 Bme1580I G_kGCm'C Cuts at: 1790 2288 2479 2621 2800 3363 4146 5302 1790 Size: 498 191 142 179 563 783 1156 2573 Fragments arranged by size: 2573 1156 783 563 498 191 179 142 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6085 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2284 3816 4774 Size: 416 53 83 399 24 867 1532 958 Cuts at: 4774 442 Size: 1753 Fragments arranged by size: 1753 1532 958 867 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2117 2528 1056 Size: 1061 411 4613 Fragments arranged by size: 4613 1061 411 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2911 2911 Size: 6085 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2754 4745 1239 Size: 1515 1991 2579 Fragments arranged by size: 2579 1991 1515 Bpu10I CC'TnA_GC Cuts at: 2396 2748 2396 Size: 352 5733 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2156 4210 5075 5373 2156 Size: 2054 865 298 2868 Fragments arranged by size: 2868 2054 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2739 3188 4748 882 Size: 617 1240 449 1560 2219 Fragments arranged by size: 2219 1560 1240 617 449 BsaAI yAC'GTr Cuts at: 493 1204 2256 3876 493 Size: 711 1052 1620 2702 Fragments arranged by size: 2702 1620 1052 711 BsaBI GATnn'nnATC Cuts at: 6079 6079 Size: 6085 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2693 3892 4274 4909 Size: 53 83 186 936 1160 1199 382 635 Cuts at: 4909 275 Size: 1451 Fragments arranged by size: 1451 1199 1160 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4518 4916 5190 5337 5748 4518 Size: 398 274 147 411 4855 Fragments arranged by size: 4855 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2837 2867 1178 Size: 30 165 30 1434 30 4396 Fragments arranged by size: 4396 1434 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2757 2900 1186 Size: 1571 143 4371 Fragments arranged by size: 4371 1571 143 BseYI C'CCAG_C Cuts at: 2479 2528 2556 3208 3312 5288 5784 2479 Size: 49 28 652 104 1976 496 2780 Fragments arranged by size: 2780 1976 652 496 104 49 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5512 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3535 4296 5324 1540 Size: 275 186 1534 761 1028 2301 Fragments arranged by size: 2301 1534 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2172 2472 2621 2662 2969 3363 4146 4231 5302 Size: 300 149 41 307 394 783 85 1071 Cuts at: 5302 2172 Size: 2955 Fragments arranged by size: 2955 1071 783 394 307 300 149 85 41 BsmI GAATG_Cn' Cuts at: 3596 3689 3596 Size: 93 5992 BsmBI CGTCTCn'nnnn_ Cuts at: 2684 3316 2684 Size: 632 5453 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2281 2288 2472 2479 2621 2662 Size: 399 382 109 7 184 7 142 41 Cuts at: 2662 2687 2756 2800 2969 3363 3404 4146 4231 Size: 25 69 44 169 394 41 742 85 Cuts at: 4231 5302 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 394 382 184 169 142 109 85 69 44 41 41 25 7 7 BspEI T'CCGG_A Cuts at: 5748 5748 Size: 6085 BspHI T'CATG_A Cuts at: 2343 3974 5704 2343 Size: 1631 1730 2724 Fragments arranged by size: 2724 1730 1631 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2032 2142 2268 3440 844 Size: 1188 110 126 1172 3489 Fragments arranged by size: 3489 1188 1172 126 110 BsrBI CCG'CTC Cuts at: 1394 1823 2515 3972 1394 Size: 429 692 1457 3507 Fragments arranged by size: 3507 1457 692 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2338 4580 4754 65 Size: 1183 1090 2242 174 1396 Fragments arranged by size: 2242 1396 1183 1090 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4729 4916 1700 Size: 110 2919 187 2869 Fragments arranged by size: 2919 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6085 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6085 BssSI C'ACGA_G Cuts at: 4145 5157 4145 Size: 1012 5073 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6085 BstBI TT'CG_AA Cuts at: 4889 4889 Size: 6085 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6085 BstXI CCAn_nnnn'nTGG Cuts at: 3118 3118 Size: 6085 BstYI r'GATC_y Cuts at: 1972 3336 3415 3782 4167 4184 5636 5722 5734 Size: 1364 79 367 385 17 1452 86 12 Cuts at: 5734 6080 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1364 385 367 346 86 79 17 12 BtgI C'CryG_G Cuts at: 513 1065 2097 2583 2856 2960 3034 513 Size: 552 1032 486 273 104 74 3564 Fragments arranged by size: 3564 1032 552 486 273 104 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5346 Fragments arranged by size: 5346 683 56 BtsI GCAGTG_nn' Cuts at: 2540 3601 4395 4415 2540 Size: 1061 794 20 4210 Fragments arranged by size: 4210 1061 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6085 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2689 2724 529 Size: 35 2125 35 3890 Fragments arranged by size: 3890 2125 35 35 DraI TTT'AAA Cuts at: 3389 3389 Size: 6085 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5092 809 Size: 507 3776 1802 Fragments arranged by size: 3776 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2325 3051 3532 Size: 54 1425 50 275 18 495 726 481 Cuts at: 3532 4421 6050 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 726 495 481 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3532 1537 Size: 275 1720 4090 Fragments arranged by size: 4090 1720 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2495 2829 2835 4015 1353 Size: 1142 334 6 1180 3423 Fragments arranged by size: 3423 1180 1142 334 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4672 5046 5192 387 Size: 1064 138 3083 374 146 1280 Fragments arranged by size: 3083 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2170 2967 2170 Size: 797 5288 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1998 2663 2754 4148 4745 5532 5800 Size: 393 366 665 91 1394 597 787 268 Cuts at: 5800 1239 Size: 1524 Fragments arranged by size: 1524 1394 787 665 597 393 366 268 91 EcoO109I rG'GnC_Cy Cuts at: 2066 2476 2797 2941 2066 Size: 410 321 144 5210 Fragments arranged by size: 5210 410 321 144 EcoRI G'AATT_C Cuts at: 3394 3772 3394 Size: 378 5707 FspI TGC'GCA Cuts at: 4591 4591 Size: 6085 HaeII r_GCGC'y Cuts at: 1678 3349 5232 1678 Size: 1671 1883 2531 Fragments arranged by size: 2531 1883 1671 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2837 2869 4726 4758 4800 Size: 32 135 32 1265 32 1857 32 42 Cuts at: 4800 4832 1373 Size: 32 2626 Fragments arranged by size: 2626 1857 1265 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2161 3232 3429 3675 669 Size: 988 281 223 1071 197 246 3079 Fragments arranged by size: 3079 1071 988 281 246 223 197 HindIII A'AGCT_T Cuts at: 748 748 Size: 6085 HpaI GTT'AAC Cuts at: 3675 3675 Size: 6085 KpnI G_GTAC'C Cuts at: 2232 3410 2232 Size: 1178 4907 MfeI C'AATT_G Cuts at: 3684 3684 Size: 6085 MluI A'CGCG_T Cuts at: 5961 5961 Size: 6085 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5199 5383 1305 Size: 3894 184 2007 Fragments arranged by size: 3894 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2327 3053 10 Size: 54 1425 343 495 726 3042 Fragments arranged by size: 3042 1425 726 495 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2582 2612 3155 4043 4402 4561 518 Size: 648 1416 30 543 888 359 159 2042 Fragments arranged by size: 2042 1416 888 648 543 359 159 30 MspA1I CmG'CkG Cuts at: 1606 2778 2823 3049 3312 3501 4178 5326 5571 Size: 1172 45 226 263 189 677 1148 245 Cuts at: 5571 1606 Size: 2120 Fragments arranged by size: 2120 1172 1148 677 263 245 226 189 45 NcoI C'CATG_G Cuts at: 513 1065 2583 2856 513 Size: 552 1518 273 3742 Fragments arranged by size: 3742 1518 552 273 NdeI CA'TA_TG Cuts at: 387 387 Size: 6085 NheI G'CTAG_C Cuts at: 1052 2113 2524 1052 Size: 1061 411 4613 Fragments arranged by size: 4613 1061 411 NotI GC'GGCC_GC Cuts at: 3532 3532 Size: 6085 NspI r_CATG'y Cuts at: 1132 1229 3443 4988 1132 Size: 97 2214 1545 2229 Fragments arranged by size: 2229 2214 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6085 PciI A'CATG_T Cuts at: 4984 4984 Size: 6085 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6085 PfoI T'CCnGG_A Cuts at: 3279 3279 Size: 6085 PmeI GTTT'AAAC Cuts at: 3389 3389 Size: 6085 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4154 4186 5667 5699 4154 Size: 32 1481 32 4540 Fragments arranged by size: 4540 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2066 2066 Size: 6085 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6085 PsiI TTA'TAA Cuts at: 3655 3655 Size: 6085 PspOMI G'GGCC_C Cuts at: 2475 2796 2475 Size: 321 5764 PstI C_TGCA'G Cuts at: 830 2814 3437 830 Size: 1984 623 3478 Fragments arranged by size: 3478 1984 623 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6085 PvuII CAG'CTG Cuts at: 2823 3049 2823 Size: 226 5859 SacI G_AGCT'C Cuts at: 2172 2969 2172 Size: 797 5288 SalI G'TCGA_C Cuts at: 1936 3427 1936 Size: 1491 4594 SanDI GG'GwC_CC Cuts at: 2066 2066 Size: 6085 SbfI CC_TGCA'GG Cuts at: 3437 3437 Size: 6085 ScaI AGT'ACT Cuts at: 4333 6045 4333 Size: 1712 4373 SexAI A'CCwGG_T Cuts at: 2502 2502 Size: 6085 SfcI C'TryA_G Cuts at: 826 1045 2810 2818 3433 4568 5249 5440 826 Size: 219 1765 8 615 1135 681 191 1471 Fragments arranged by size: 1765 1471 1135 681 615 219 191 8 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6085 SmaI CCC'GGG Cuts at: 1872 3412 1872 Size: 1540 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2171 4189 5090 5352 820 Size: 197 926 228 2018 901 262 1553 Fragments arranged by size: 2018 1553 926 901 262 228 197 SnaBI TAC'GTA Cuts at: 493 3876 493 Size: 3383 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6085 SphI G_CATG'C Cuts at: 1132 3443 1132 Size: 2311 3774 SspI AAT'ATT Cuts at: 5 52 4009 5 Size: 47 3957 2081 Fragments arranged by size: 3957 2081 47 StuI AGG'CCT Cuts at: 1517 2816 1517 Size: 1299 4786 StyI C'CwwG_G Cuts at: 513 1065 2583 2856 3524 513 Size: 552 1518 273 668 3074 Fragments arranged by size: 3074 1518 668 552 273 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4112 4129 4282 4467 4912 325 Size: 1255 2532 17 153 185 445 1498 Fragments arranged by size: 2532 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4331 6043 96 Size: 274 80 33 51 1443 2354 1712 138 Fragments arranged by size: 2354 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3091 3123 6045 6077 3091 Size: 32 2922 32 3099 Fragments arranged by size: 3099 2922 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6085 XbaI T'CTAG_A Cuts at: 3421 3421 Size: 6085 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3032 3032 Size: 6085 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6085 XmaI C'CCGG_G Cuts at: 1870 3410 1870 Size: 1540 4545 XmnI GAAnn'nnTTC Cuts at: 3065 4214 3065 Size: 1149 4936 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3893 276 Size: 53 83 186 936 2359 2468 Fragments arranged by size: 2468 2359 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI