(Circular) (Six-base) MAPSORT of: pFN21ASDA0142.seq Check: 6153 from: 1 to: 6667 pFN21ASDA0142 6667 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2118 4477 278 Size: 53 83 186 936 582 2359 2468 Fragments arranged by size: 2468 2359 936 582 186 83 53 AccI GT'mk_AC Cuts at: 1937 4010 1937 Size: 2073 4594 Acc65I G'GTAC_C Cuts at: 3988 3988 Size: 6667 AclI AA'CG_TT Cuts at: 1563 4794 5167 1563 Size: 3231 373 3063 Fragments arranged by size: 3231 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2273 2342 3552 3941 4730 6114 6382 1632 Size: 641 69 1210 389 789 1384 268 1917 Fragments arranged by size: 1917 1384 1210 789 641 389 268 69 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6470 AflIII A'CryG_T Cuts at: 1203 2934 5566 6543 1203 Size: 1731 2632 977 1327 Fragments arranged by size: 2632 1731 1327 977 AgeI A'CCGG_T Cuts at: 5498 5498 Size: 6667 AhdI GACnn_n'nnGTC Cuts at: 5396 5396 Size: 6667 AleI CACnn'nnGTG Cuts at: 1166 2132 3230 1166 Size: 966 1098 4603 Fragments arranged by size: 4603 1098 966 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2216 2519 1184 Size: 1032 303 5332 Fragments arranged by size: 5332 1032 303 AlwNI CAG_nnn'CTG Cuts at: 2872 2947 3184 3628 4410 5982 2872 Size: 75 237 444 782 1572 3557 Fragments arranged by size: 3557 1572 782 444 237 75 ApaLI G'TGCA_C Cuts at: 4724 5880 4724 Size: 1156 5511 ApoI r'AATT_y Cuts at: 1493 3976 4202 4354 6463 1493 Size: 2483 226 152 2109 1697 Fragments arranged by size: 2483 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5221 160 Size: 872 4189 1606 Fragments arranged by size: 4189 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6667 AvaI C'yCGr_G Cuts at: 1870 1943 2747 3992 1870 Size: 73 804 1245 4545 Fragments arranged by size: 4545 1245 804 73 AvrII C'CTAG_G Cuts at: 3032 3032 Size: 6667 BamHI G'GATC_C Cuts at: 3997 4364 3997 Size: 367 6300 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2657 3377 3988 5443 618 Size: 325 217 627 870 720 611 1455 1842 Fragments arranged by size: 1842 1455 870 720 627 611 325 217 BanII G_rGCy'C Cuts at: 1391 2261 2748 3518 3886 3986 1391 Size: 870 487 770 368 100 4072 Fragments arranged by size: 4072 870 770 487 368 100 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5696 BbvCI CC'TCA_GC Cuts at: 3478 3478 Size: 6667 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4858 4892 1903 Size: 34 2921 34 3678 Fragments arranged by size: 3678 2921 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3790 4559 5769 3790 Size: 769 1210 4688 Fragments arranged by size: 4688 1210 769 BclI T'GATC_A Cuts at: 1548 1548 Size: 6667 BfrBI ATG'CAT Cuts at: 3122 3809 3122 Size: 687 5980 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2178 5278 136 Size: 107 122 71 631 605 506 3100 1525 Fragments arranged by size: 3100 1525 631 605 506 122 107 71 BglII A'GATC_T Cuts at: 6662 6662 Size: 6667 BlpI GC'TnA_GC Cuts at: 2514 4084 2514 Size: 1570 5097 Bme1580I G_kGCm'C Cuts at: 1790 2162 4728 5884 1790 Size: 372 2566 1156 2573 Fragments arranged by size: 2573 2566 1156 372 BmgBI CAC'GTC Cuts at: 1318 3228 1318 Size: 1910 4757 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2902 3409 4398 Size: 416 53 83 399 24 1485 507 989 Cuts at: 4398 5356 442 Size: 958 1753 Fragments arranged by size: 1753 1485 989 958 507 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6667 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3431 3457 3917 5327 1239 Size: 2192 26 460 1410 2579 Fragments arranged by size: 2579 2192 1410 460 26 Bpu10I CC'TnA_GC Cuts at: 2696 3443 3478 2696 Size: 747 35 5885 Fragments arranged by size: 5885 747 35 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2879 3116 4792 5657 5955 2879 Size: 237 1676 865 298 3591 Fragments arranged by size: 3591 1676 865 298 237 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2642 3944 5330 882 Size: 617 1143 1302 1386 2219 Fragments arranged by size: 2219 1386 1302 1143 617 BsaAI yAC'GTr Cuts at: 493 1204 2200 2577 3964 4458 493 Size: 711 996 377 1387 494 2702 Fragments arranged by size: 2702 1387 996 711 494 377 BsaBI GATnn'nnATC Cuts at: 6661 6661 Size: 6667 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2115 3338 4474 4856 Size: 53 83 186 936 582 1223 1136 382 Cuts at: 4856 5491 275 Size: 635 1451 Fragments arranged by size: 1451 1223 1136 936 635 582 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2841 2886 5100 5498 5772 5919 6330 2841 Size: 45 2214 398 274 147 411 3178 Fragments arranged by size: 3178 2214 411 398 274 147 45 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3569 3599 1178 Size: 30 165 30 2166 30 4246 Fragments arranged by size: 4246 2166 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2642 3590 1186 Size: 1456 948 4263 Fragments arranged by size: 4263 1456 948 BseYI C'CCAG_C Cuts at: 2189 5870 6366 2189 Size: 3681 496 2490 Fragments arranged by size: 3681 2490 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3634 1163 Size: 573 1898 4196 Fragments arranged by size: 4196 1898 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2173 4117 4878 5906 1540 Size: 275 186 172 1944 761 1028 2301 Fragments arranged by size: 2301 1944 1028 761 275 186 172 BsiHKAI G_wGCw'C Cuts at: 2748 3204 4728 4813 5884 2748 Size: 456 1524 85 1071 3531 Fragments arranged by size: 3531 1524 1071 456 85 BsmI GAATG_Cn' Cuts at: 2498 4178 4271 2498 Size: 1680 93 4894 Fragments arranged by size: 4894 1680 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2106 2106 Size: 6667 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2162 2261 2748 3204 3518 3886 3986 Size: 399 372 99 487 456 314 368 100 Cuts at: 3986 4728 4813 5884 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 487 456 399 372 368 314 100 99 85 BspEI T'CCGG_A Cuts at: 2841 2886 6330 2841 Size: 45 3444 3178 Fragments arranged by size: 3444 3178 45 BspHI T'CATG_A Cuts at: 4556 6286 4556 Size: 1730 4937 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4022 844 Size: 3178 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3151 4554 1394 Size: 429 1328 1403 3507 Fragments arranged by size: 3507 1403 1328 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2387 5162 5336 65 Size: 1183 1139 2775 174 1396 Fragments arranged by size: 2775 1396 1183 1139 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2177 5311 5498 1700 Size: 110 367 3134 187 2869 Fragments arranged by size: 3134 2869 367 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6667 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6667 BssSI C'ACGA_G Cuts at: 2678 4727 5739 2678 Size: 2049 1012 3606 Fragments arranged by size: 3606 2049 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3184 794 Size: 2390 4277 BstBI TT'CG_AA Cuts at: 2007 5471 2007 Size: 3464 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6667 BstYI r'GATC_y Cuts at: 1972 2868 3181 3502 3997 4364 4749 4766 6218 Size: 896 313 321 495 367 385 17 1452 Cuts at: 6218 6304 6316 6662 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 896 495 385 367 346 321 313 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2688 2799 3373 513 Size: 552 1623 111 574 3807 Fragments arranged by size: 3807 1623 574 552 111 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2916 487 Size: 683 56 1690 4238 Fragments arranged by size: 4238 1690 683 56 BtsI GCAGTG_nn' Cuts at: 2607 4183 4977 4997 2607 Size: 1576 794 20 4277 Fragments arranged by size: 4277 1576 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6667 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3696 3731 529 Size: 35 3132 35 3465 Fragments arranged by size: 3465 3132 35 35 DraI TTT'AAA Cuts at: 2541 3971 2541 Size: 1430 5237 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5674 809 Size: 507 4358 1802 Fragments arranged by size: 4358 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2170 2384 4114 Size: 54 1425 50 275 18 340 214 1730 Cuts at: 4114 5003 6632 8 Size: 889 1629 43 Fragments arranged by size: 1730 1629 1425 889 340 275 214 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2170 4114 1537 Size: 275 358 1944 4090 Fragments arranged by size: 4090 1944 358 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2133 2249 2840 3002 3723 4597 1353 Size: 780 116 591 162 721 874 3423 Fragments arranged by size: 3423 874 780 721 591 162 116 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5254 5628 5774 387 Size: 1064 138 3665 374 146 1280 Fragments arranged by size: 3665 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2746 2746 Size: 6667 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 2342 3431 3457 3552 3917 3941 Size: 393 641 69 1089 26 95 365 24 Cuts at: 3941 4730 5327 6114 6382 1239 Size: 789 597 787 268 1524 Fragments arranged by size: 1524 1089 789 787 641 597 393 365 268 95 69 26 24 EcoNI CCTnn'n_nnAGG Cuts at: 2265 2265 Size: 6667 EcoO109I rG'GnC_Cy Cuts at: 2944 3404 2944 Size: 460 6207 EcoRI G'AATT_C Cuts at: 3976 4354 3976 Size: 378 6289 EcoRV GAT'ATC Cuts at: 2987 2987 Size: 6667 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2086 3734 2086 Size: 1648 5019 FspI TGC'GCA Cuts at: 5173 5173 Size: 6667 HaeII r_GCGC'y Cuts at: 1678 3660 5814 1678 Size: 1982 2154 2531 Fragments arranged by size: 2531 2154 1982 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2103 2135 3569 3601 5308 Size: 32 135 32 531 32 1434 32 1707 Cuts at: 5308 5340 5382 5414 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1707 1434 531 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4011 4257 669 Size: 988 281 2073 246 3079 Fragments arranged by size: 3079 2073 988 281 246 HindIII A'AGCT_T Cuts at: 748 3062 3092 3642 748 Size: 2314 30 550 3773 Fragments arranged by size: 3773 2314 550 30 HpaI GTT'AAC Cuts at: 4257 4257 Size: 6667 KpnI G_GTAC'C Cuts at: 3992 3992 Size: 6667 MfeI C'AATT_G Cuts at: 4266 4266 Size: 6667 MluI A'CGCG_T Cuts at: 6543 6543 Size: 6667 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2506 3092 5781 5965 1305 Size: 1201 586 2689 184 2007 Fragments arranged by size: 2689 2007 1201 586 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4845 Fragments arranged by size: 4845 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2132 2933 3117 3230 4625 4984 5143 Size: 648 966 801 184 113 1395 359 159 Cuts at: 5143 518 Size: 2042 Fragments arranged by size: 2042 1395 966 801 648 359 184 159 113 MspA1I CmG'CkG Cuts at: 1606 3334 3482 3602 4083 4760 5908 6153 1606 Size: 1728 148 120 481 677 1148 245 2120 Fragments arranged by size: 2120 1728 1148 677 481 245 148 120 NcoI C'CATG_G Cuts at: 513 1065 2799 513 Size: 552 1734 4381 Fragments arranged by size: 4381 1734 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6667 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6667 NotI GC'GGCC_GC Cuts at: 4114 4114 Size: 6667 NsiI A_TGCA'T Cuts at: 3124 3811 3124 Size: 687 5980 NspI r_CATG'y Cuts at: 1132 1229 2938 4025 5570 1132 Size: 97 1709 1087 1545 2229 Fragments arranged by size: 2229 1709 1545 1087 97 PasI CC'CwG_GG Cuts at: 1348 2669 1348 Size: 1321 5346 PciI A'CATG_T Cuts at: 2934 5566 2934 Size: 2632 4035 PflMI CCAn_nnn'nTGG Cuts at: 1108 3399 3435 1108 Size: 2291 36 4340 Fragments arranged by size: 4340 2291 36 PfoI T'CCnGG_A Cuts at: 3587 3587 Size: 6667 PmeI GTTT'AAAC Cuts at: 3971 3971 Size: 6667 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4736 4768 6249 6281 4736 Size: 32 1481 32 5122 Fragments arranged by size: 5122 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2944 3404 2944 Size: 460 6207 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6667 PsiI TTA'TAA Cuts at: 4237 4237 Size: 6667 PstI C_TGCA'G Cuts at: 830 2602 2868 3189 4019 830 Size: 1772 266 321 830 3478 Fragments arranged by size: 3478 1772 830 321 266 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6667 PvuII CAG'CTG Cuts at: 3482 3602 3482 Size: 120 6547 RsrII CG'GwC_CG Cuts at: 2175 2175 Size: 6667 SacI G_AGCT'C Cuts at: 2748 2748 Size: 6667 SalI G'TCGA_C Cuts at: 1936 4009 1936 Size: 2073 4594 SanDI GG'GwC_CC Cuts at: 3404 3404 Size: 6667 SapI GCTCTTCn'nnn_ Cuts at: 2249 2249 Size: 6667 SbfI CC_TGCA'GG Cuts at: 4019 4019 Size: 6667 ScaI AGT'ACT Cuts at: 4915 6627 4915 Size: 1712 4955 SfcI C'TryA_G Cuts at: 826 1045 2315 2598 2864 3185 3206 4015 5150 Size: 219 1270 283 266 321 21 809 1135 Cuts at: 5150 5831 6022 826 Size: 681 191 1471 Fragments arranged by size: 1471 1270 1135 809 681 321 283 266 219 191 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6667 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6667 SmaI CCC'GGG Cuts at: 1872 3994 1872 Size: 2122 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2747 2858 3095 4771 5672 5934 Size: 197 926 804 111 237 1676 901 262 Cuts at: 5934 820 Size: 1553 Fragments arranged by size: 1676 1553 926 901 804 262 237 197 111 SnaBI TAC'GTA Cuts at: 493 3964 4458 493 Size: 3471 494 2702 Fragments arranged by size: 3471 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6667 SphI G_CATG'C Cuts at: 1132 4025 1132 Size: 2893 3774 SspI AAT'ATT Cuts at: 5 52 2323 2345 4591 5 Size: 47 2271 22 2246 2081 Fragments arranged by size: 2271 2246 2081 47 22 StuI AGG'CCT Cuts at: 1517 1517 Size: 6667 StyI C'CwwG_G Cuts at: 513 1065 2799 3032 4106 513 Size: 552 1734 233 1074 3074 Fragments arranged by size: 3074 1734 1074 552 233 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2042 3184 3442 4694 4711 4864 5049 Size: 1255 462 1142 258 1252 17 153 185 Cuts at: 5049 5494 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1252 1142 462 445 258 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4913 6625 96 Size: 274 80 33 51 1443 2936 1712 138 Fragments arranged by size: 2936 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6627 6659 6627 Size: 32 6635 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6667 XbaI T'CTAG_A Cuts at: 2443 3865 4003 2443 Size: 1422 138 5107 Fragments arranged by size: 5107 1422 138 XhoI C'TCGA_G Cuts at: 1943 2747 1943 Size: 804 5863 XmaI C'CCGG_G Cuts at: 1870 3992 1870 Size: 2122 4545 XmnI GAAnn'nnTTC Cuts at: 4796 4796 Size: 6667 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2116 4475 276 Size: 53 83 186 936 582 2359 2468 Fragments arranged by size: 2468 2359 936 582 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BplI BsiWI BstXI BstZ17I Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI SacII SexAI SfiI SfoI SrfI SwaI XcmI