ARHGAP25 ORF clone pF1KSDA0053

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Flexi ORF Clone pF1KSDA0053

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Clone info.

Species

human

Clone Name

pF1KSDA0053 Sequence (with vector) Restriction Map

Product ID

FXC00387

HaloTag Clone PID

FHC00387

ORF Sequence

DNA sequence (1914 bp)
Protein sequence (638 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB383728]

Gene

KIAA0053

Symbol

ARHGAP25

Alias : HEL-S-308, KAIA0053

Description

Rho GTPase activating protein 25, transcript variant 2

Original Clone ID

ha01417

Features of the cloned ORF sequence

Description

Length: 1914 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 638 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS46312.1 3.9e-153 100.0 ARHGAP25 CCDS54364.1 1.1e-152 99.8 ARHGAP25 CCDS33214.2 9.1e-150 99.8 ARHGAP25 CCDS54363.1 4.1e-115 93.8 ARHGAP25 CCDS7227.1 5.3e-45 42.9 ARHGAP22 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001849 41 142 PF00169 Pleckstrin homology domain IPR000198 170 318 PF00620 Rho GTPase-activating protein domain HMMSmart IPR001849 40 146 SM00233 Pleckstrin homology domain IPR000198 167 343 SM00324 Rho GTPase-activating protein domain ProfileScan IPR001849 39 144 PS50003 Pleckstrin homology domain IPR000198 152 346 PS50238 Rho GTPase-activating protein domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055697 638 610587 100.0 100.0 NP_001159748 639 610587 99.8 100.0 XP_016860913 649 610587 98.7 99.4 XP_005264732 645 610587 100.0 98.1 XP_016860912 650 610587 98.6 99.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KSDA0053 Download

>KIAA0053  1914 bp
ATGTCCCTCGGTCAGTCGGCCTGTCTGTTCCTCTCTATAGCTCGGTCAAGGAGTGTGATG
ACTGGCGAGCAGATGGCTGCCTTCCATCCATCGTCCACCCCCAACCCGCTGGAGAGGCCC
ATCAAGATGGGCTGGCTGAAGAAGCAGAGGTCCATCGTGAAGAACTGGCAGCAGAGGTAC
TTTGTGCTGAGGGCGCAGCAGCTCTACTACTACAAGGATGAAGAGGACACGAAGCCCCAG
GGCTGCATGTATCTACCAGGATGTACAATCAAGGAGATCGCCACAAACCCAGAAGAAGCT
GGGAAGTTTGTCTTTGAAATCATTCCAGCCTCATGGGACCAGAATCGCATGGGACAGGAC
TCCTATGTCCTCATGGCCAGCTCTCAGGCGGAGATGGAGGAGTGGGTTAAATTCCTCAGG
AGAGTTGCTGGCACACCCTGTGGAGTGTTTGGCCAGCGCTTGGATGAGACTGTGGCCTAT
GAACAGAAATTCGGCCCCCATCTGGTGCCCATCCTGGTGGAGAAATGTGCAGAGTTCATC
CTGGAGCACGGCCGGAATGAAGAGGGCATCTTCCGTCTGCCTGGGCAGGACAACCTGGTG
AAGCAGCTGAGAGACGCTTTTGATGCTGGGGAGCGGCCCTCCTTTGACAGAGACACAGAT
GTGCACACTGTGGCTTCCCTGTTAAAGCTCTACCTCCGAGACCTCCCAGAGCCCGTGGTT
CCCTGGAGCCAGTACGAAGGGTTCCTGCTCTGTGGGCAGCTCACGAATGCGGATGAGGCA
AAGGCTCAGCAGGAGTTGATGAAGCAGCTCTCCATCCTTCCTCGTGACAACTATAGTCTC
CTGAGCTACATCTGCAGGTTCCTACATGAAATACAGCTGAACTGTGCTGTTAACAAGATG
AGTGTGGACAACCTGGCTACTGTGATTGGTGTGAATCTCATCAGGTCGAAGGTCGAAGAC
CCTGCCGTGATCATGAGAGGGACTCCTCAGATCCAAAGAGTGATGACTATGATGATCAGA
GACCATGAAGTCCTCTTCCCCAAGTCCAAGGATATACCCCTGTCACCCCCTGCCCAGAAA
AATGACCCCAAGAAAGCTCCAGTGGCCCGAAGCTCTGTAGGCTGGGATGCCACTGAAGAC
CTCCGAATTTCTAGGACAGACAGCTTCAGTAGCATGACAAGCGACTCTGATACAACCAGC
CCCACCGGACAGCAGCCGAGCGATGCGTTTCCGGAGGACAGCAGCAAAGTACCCAGGGAA
AAGCCAGGAGACTGGAAAATGCAATCTCGTAAAAGGACTCAAACACTCCCTAACCGGAAA
TGTTTCTTGACATCAGCTTTTCAGGGTGCCAACAGCAGCAAAATGGAGATCTTTAAAAAT
GAATTCTGGTCGCCTTCCTCAGAGGCTAAGGCAGGGGAAGGGCACAGGAGAACGATGTCT
CAAGACTTGCGCCAACTTTCTGACTCCCAACGGACTTCCACCTACGATAACGTCCCTTCC
CTGCCAGGGTCCCCTGGGGAGGAAGCCAGTGCACTCTCTTCCCAAGCCTGTGACTCCAAG
GGAGATACTCTTGCCAGTCCAAACTCTGAAACTGGGCCTGGAAAAAAGAACTCTGGAGAA
GAGGAAATTGATTCTTTGCAGAGGATGGTCCAAGAGCTACGAAAGGAAATAGAAACACAG
AAGCAAATGTATGAGGAACAGATTAAAAACCTTGAGAAGGAAAATTATGACGTTTGGGCT
AAAGTGGTGAGGCTCAATGAAGAACTGGAGAAGGAAAAGAAGAAGTCTGCAGCCCTAGAG
ATCAGCCTCCGCAACATGGAGCGCTCCCGGGAGGATGTTGAGAAGAGGAACAAGGCCTTG
GAAGAAGAAGTCAAGGAATTTGTCAAATCCATGAAGGAACCCAAGACCGAGGCT

ORF Protein

Cloned ORF protein sequence for pF1KSDA0053 Download

>KIAA0053  638 aa
MSLGQSACLFLSIARSRSVMTGEQMAAFHPSSTPNPLERPIKMGWLKKQRSIVKNWQQRY
FVLRAQQLYYYKDEEDTKPQGCMYLPGCTIKEIATNPEEAGKFVFEIIPASWDQNRMGQD
SYVLMASSQAEMEEWVKFLRRVAGTPCGVFGQRLDETVAYEQKFGPHLVPILVEKCAEFI
LEHGRNEEGIFRLPGQDNLVKQLRDAFDAGERPSFDRDTDVHTVASLLKLYLRDLPEPVV
PWSQYEGFLLCGQLTNADEAKAQQELMKQLSILPRDNYSLLSYICRFLHEIQLNCAVNKM
SVDNLATVIGVNLIRSKVEDPAVIMRGTPQIQRVMTMMIRDHEVLFPKSKDIPLSPPAQK
NDPKKAPVARSSVGWDATEDLRISRTDSFSSMTSDSDTTSPTGQQPSDAFPEDSSKVPRE
KPGDWKMQSRKRTQTLPNRKCFLTSAFQGANSSKMEIFKNEFWSPSSEAKAGEGHRRTMS
QDLRQLSDSQRTSTYDNVPSLPGSPGEEASALSSQACDSKGDTLASPNSETGPGKKNSGE
EEIDSLQRMVQELRKEIETQKQMYEEQIKNLEKENYDVWAKVVRLNEELEKEKKKSAALE
ISLRNMERSREDVEKRNKALEEEVKEFVKSMKEPKTEA

ORF+Vector

Nucleotide Sequence (with vector) for pF1KSDA0053 Download

>pF1KSDA0053  5051 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCTTTCGAAGGAGAT
AGAACCATGTCCCTCGGTCAGTCGGCCTGTCTGTTCCTCTCTATAGCTCGGTCAAGGAGT
GTGATGACTGGCGAGCAGATGGCTGCCTTCCATCCATCGTCCACCCCCAACCCGCTGGAG
AGGCCCATCAAGATGGGCTGGCTGAAGAAGCAGAGGTCCATCGTGAAGAACTGGCAGCAG
AGGTACTTTGTGCTGAGGGCGCAGCAGCTCTACTACTACAAGGATGAAGAGGACACGAAG
CCCCAGGGCTGCATGTATCTACCAGGATGTACAATCAAGGAGATCGCCACAAACCCAGAA
GAAGCTGGGAAGTTTGTCTTTGAAATCATTCCAGCCTCATGGGACCAGAATCGCATGGGA
CAGGACTCCTATGTCCTCATGGCCAGCTCTCAGGCGGAGATGGAGGAGTGGGTTAAATTC
CTCAGGAGAGTTGCTGGCACACCCTGTGGAGTGTTTGGCCAGCGCTTGGATGAGACTGTG
GCCTATGAACAGAAATTCGGCCCCCATCTGGTGCCCATCCTGGTGGAGAAATGTGCAGAG
TTCATCCTGGAGCACGGCCGGAATGAAGAGGGCATCTTCCGTCTGCCTGGGCAGGACAAC
CTGGTGAAGCAGCTGAGAGACGCTTTTGATGCTGGGGAGCGGCCCTCCTTTGACAGAGAC
ACAGATGTGCACACTGTGGCTTCCCTGTTAAAGCTCTACCTCCGAGACCTCCCAGAGCCC
GTGGTTCCCTGGAGCCAGTACGAAGGGTTCCTGCTCTGTGGGCAGCTCACGAATGCGGAT
GAGGCAAAGGCTCAGCAGGAGTTGATGAAGCAGCTCTCCATCCTTCCTCGTGACAACTAT
AGTCTCCTGAGCTACATCTGCAGGTTCCTACATGAAATACAGCTGAACTGTGCTGTTAAC
AAGATGAGTGTGGACAACCTGGCTACTGTGATTGGTGTGAATCTCATCAGGTCGAAGGTC
GAAGACCCTGCCGTGATCATGAGAGGGACTCCTCAGATCCAAAGAGTGATGACTATGATG
ATCAGAGACCATGAAGTCCTCTTCCCCAAGTCCAAGGATATACCCCTGTCACCCCCTGCC
CAGAAAAATGACCCCAAGAAAGCTCCAGTGGCCCGAAGCTCTGTAGGCTGGGATGCCACT
GAAGACCTCCGAATTTCTAGGACAGACAGCTTCAGTAGCATGACAAGCGACTCTGATACA
ACCAGCCCCACCGGACAGCAGCCGAGCGATGCGTTTCCGGAGGACAGCAGCAAAGTACCC
AGGGAAAAGCCAGGAGACTGGAAAATGCAATCTCGTAAAAGGACTCAAACACTCCCTAAC
CGGAAATGTTTCTTGACATCAGCTTTTCAGGGTGCCAACAGCAGCAAAATGGAGATCTTT
AAAAATGAATTCTGGTCGCCTTCCTCAGAGGCTAAGGCAGGGGAAGGGCACAGGAGAACG
ATGTCTCAAGACTTGCGCCAACTTTCTGACTCCCAACGGACTTCCACCTACGATAACGTC
CCTTCCCTGCCAGGGTCCCCTGGGGAGGAAGCCAGTGCACTCTCTTCCCAAGCCTGTGAC
TCCAAGGGAGATACTCTTGCCAGTCCAAACTCTGAAACTGGGCCTGGAAAAAAGAACTCT
GGAGAAGAGGAAATTGATTCTTTGCAGAGGATGGTCCAAGAGCTACGAAAGGAAATAGAA
ACACAGAAGCAAATGTATGAGGAACAGATTAAAAACCTTGAGAAGGAAAATTATGACGTT
TGGGCTAAAGTGGTGAGGCTCAATGAAGAACTGGAGAAGGAAAAGAAGAAGTCTGCAGCC
CTAGAGATCAGCCTCCGCAACATGGAGCGCTCCCGGGAGGATGTTGAGAAGAGGAACAAG
GCCTTGGAAGAAGAAGTCAAGGAATTTGTCAAATCCATGAAGGAACCCAAGACCGAGGCT
TACGTAGTTTAAACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGG
CATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCAC
CGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTT
GCTGAAAGGAGGAACTATATCCGGTTCGCTTGCTGTCCATAAAACCGCCCAGTCTAGCTA
TCGCCATGTAAGCCCACTGCAAGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTCCCTTGT
CCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTT
CTACGTGTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCGTGAGCT
TCAAAAGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAAC
TGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAG
ACAGGATGACGGTCGTTTCGCATGCTTGAACAAGATGGATTGCACGCAGGTTCTCCGGCC
GCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGAT
GCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTG
TCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACG
GGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTA
TTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTA
TCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTC
GACCACCAAGCGAAACATCGCATCGAGCGAGCACGCACTCGGATGGAAGCCGGTCTTGTC
GATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGG
CTCAAGGCGCGTATGCCGGATGGTGAGGATCTCGTCGTGACTCATGGCGATGCCTGCTTG
CCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGT
GTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGC
GGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGC
ATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGA
CCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCAC
AGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAA
CCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCA
CAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGC
GTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATA
CCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTA
TCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCA
GCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGA
CTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGG
TGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGG
TATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGG
CAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAG
AAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAA
CGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGAT
CCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAA
ACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGG
TTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTG
GTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGC
GTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAA
ACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTTTTCAGCCTGAT
ACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAG
CGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGG
TAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGG
CTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGA
GTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGC
GGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGG
ATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCAT