Restriction map for pF1KSDA0053
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
AlwNI       ApaI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbeI        BbrPI       BciVI       BfrI        
BglI        BlnI        BsaAI       BsaBI       BsaHI       
BsiWI       BspDI       BspLU11I    BsrDI       BsrFI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     Cfr10I      ClaI        
CpoI        Eam1105I    EcoO65I     EcoRV       EcoT22I     
FseI        FspI        Hin1I       HindIII     KasI        
KpnI        KspI        MamI        MfeI        MluI        
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
Tth111I     XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccIII       1230  TCCGGA               T^CCGGA
AlwI          990  GATCC                GGATC(4/5)
AocI          414  CCTCAGG              CC^T[ACGT]AGG
AvaI         1826  CCCGGG               C^[CT]CG[GA]G
BanII         709  GAGCCC               G[GA]GC[CT]^C
BbvCI         186  GCTGAGG              CCTCAGC(-5/-2)
BcgI          954  CGAAGACCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1367  AGATCT               A^GATCT
BlpI          784  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      784  GCTCAGC              GC^T[ACGT]AGC
BseAI        1230  TCCGGA               T^CCGGA
BsgI          527  GTGCAG               GTGCAG(16/14)
BsiEI         549  CGGCCG               CG[GA][CT]^CG
BsmBI         611  GAGACG               CGTCTC(1/5)
BsmI          765  GAATGC               GAATGC(1/-1)
BsoBI        1826  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      262  TGTACA               T^GTACA
BspEI        1230  TCCGGA               T^CCGGA
BspHI         971  TCATGA               T^CATGA
BspMI         854  GCAGGT               ACCTGC(4/8)
BsrBI         631  GAGCGG               CCGCTC(-3/-3)
BsrGI         262  TGTACA               T^GTACA
BssSI         821  CTCGTG               CACGAG(-5/-1)
Bsu36I        414  CCTCAGG              CC^T[ACGT]AGG
CelII         784  GCTCAGC              GC^T[ACGT]AGC
DraI         1372  TTTAAA               TTT^AAA
DraII        1507  GGGTCCC              [GA]G^G[ACGT]CC[CT]
DsaI          713  CCGTGG               C^C[GA][CT]GG
EagI          549  CGGCCG               C^GGCCG
EclXI         549  CGGCCG               C^GGCCG
Eco52I        549  CGGCCG               C^GGCCG
Eco81I        414  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1710  CCTTGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1507  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRI        1381  GAATTC               G^AATTC
FauI          105  CCCGC                CCCGC(4/6)
HgaI          613  GACGC                GACGC(5/10)
HincII        889  GTTAAC               GT[CT]^[GA]AC
HindII        889  GTTAAC               GT[CT]^[GA]AC
HpaI          889  GTTAAC               GTT^AAC
MroI         1230  TCCGGA               T^CCGGA
NspI          245  GCATGT               [GA]CATG^[CT]
PflMI         453  CCAGCGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1507  GGGTCCC              [GA]G^G[AT]CC[CT]
RcaI          971  TCATGA               T^CATGA
SexAI         593  ACCTGGT              A^CC[AT]GGT
SmaI         1826  CCCGGG               CCC^GGG
SspBI         262  TGTACA               T^GTACA
StuI         1853  AGGCCT               AGG^CCT
Van91I        453  CCAGCGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        1826  CCCGGG               C^CCGGG
XmaI         1826  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

Alw44I        661  GTGCAC               G^TGCAC
             1529  GTGCAC              
Aor51HI       455  AGCGCT               AGC^GCT
             1820  AGCGCT              
ApaLI         661  GTGCAC               G^TGCAC
             1529  GTGCAC              
Asp700I       316  GAAATCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1317  GAAATGTTTC          
BalI          374  TGGCCA               TGG^CCA
              450  TGGCCA              
BanI          504  GGTGCC               G^G[CT][GA]CC
             1345  GGTGCC              
BbsI          955  GAAGAC               GAAGAC(2/6)
             1135  GAAGAC              
BclI          968  TGATCA               T^GATCA
             1013  TGATCA              
BcnI         1826  CCCGG                CC^[GC]GG
             1827  CCGGG               
BfaI         1151  CTAG                 C^TAG
             1795  CTAG                
BpuAI         955  GAAGAC               GAAGAC(2/6)
             1135  GAAGAC              
BsaI          698  GAGACC               GGTCTC(1/5)
             1019  GAGACC              
BseRI         983  CTCCTC               GAGGAG(10/8)
              397  GAGGAG              
BstYI         989  AGATCC               [GA]^GATC[CT]
             1367  AGATCT              
DraIII        434  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
              664  CACACTGTG           
DrdI          358  GACTCCTATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1021  GACCATGAAGTC        
Eco47III      455  AGCGCT               AGC^GCT
             1820  AGCGCT              
FbaI          968  TGATCA               T^GATCA
             1013  TGATCA              
HaeII         455  AGCGCT               [GA]GCGC^[CT]
             1820  AGCGCT              
MaeI         1151  CTAG                 C^TAG
             1795  CTAG                
MaeII        1490  ACGT                 A^CGT
             1730  ACGT                
MflI          989  AGATCC               [GA]^GATC[CT]
             1367  AGATCT              
MluNI         374  TGGCCA               TGG^CCA
              450  TGGCCA              
MscI          374  TGGCCA               TGG^CCA
              450  TGGCCA              
NciI         1826  CCCGG                CC^[GC]GG
             1827  CCGGG               
PstI          852  CTGCAG               CTGCA^G
             1787  CTGCAG              
PvuII         604  CAGCTG               CAG^CTG
              874  CAGCTG              
SmlI         1439  CTCAAG               C^T[CT][GA]AG
             1711  CTTGAG              
TaqI          946  TCGA                 T^CGA
              953  TCGA                
TthHB8I       946  TCGA                 T^CGA
              953  TCGA                
XhoII         989  AGATCC               [GA]^GATC[CT]
             1367  AGATCT              
XmnI          316  GAAATCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1317  GAAATGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AspHI       AvaII       BbvI        BmyI        BpmI        
BsaJI       BsaWI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstXI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EaeI        EarI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeIII      MboI        
MboII       MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
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