GIT2 ORF clone FHC00029

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00029

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Clone info.

Species

human

Clone Name

pFN21AA0148 Sequence (with vector) Restriction Map

Product ID

FHC00029

Flexi Clone PID

FXC00029

ORF Sequence

DNA sequence (2043 bp)
Protein sequence (681 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385285]

Gene

KIAA0148

Symbol

GIT2

Alias : CAT-2, CAT2, PKL

Description

G protein-coupled receptor kinase interacting ArfGAP 2, transcript variant 6

Original Clone ID

fh23975

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2043 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 681 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS55884.1 1.2e-180 100.0 GIT2 CCDS81736.1 2.5e-130 95.4 GIT2 CCDS44969.1 6.7e-108 89.1 GIT2 CCDS81737.1 1.1e-107 99.3 GIT2 CCDS9139.1 1.4e-107 97.7 GIT2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001164 8 27 PR00405 Arf GTPase activating protein IPR001164 27 44 PR00405 Arf GTPase activating protein IPR001164 48 69 PR00405 Arf GTPase activating protein HMMPfam IPR001164 6 118 PF01412 Arf GTPase activating protein IPR013724 268 298 PF08518 Spa2 homology (SHD) of GIT IPR013724 332 361 PF08518 Spa2 homology (SHD) of GIT IPR022018 555 676 PF12205 G protein-coupled receptor kinase-interacting protein 1 C term HMMSmart IPR001164 1 124 SM00105 Arf GTPase activating protein IPR002110 132 161 SM00248 Ankyrin repeat IPR002110 166 195 SM00248 Ankyrin repeat IPR002110 199 228 SM00248 Ankyrin repeat IPR013724 268 298 SM00555 Spa2 homology (SHD) of GIT IPR013724 332 362 SM00555 Spa2 homology (SHD) of GIT ProfileScan IPR001164 1 124 PS50115 Arf GTPase activating protein IPR020683 132 221 PS50297 Ankyrin repeat-containing domain IPR002110 166 198 PS50088 Ankyrin repeat
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001128685 681 608564 100.0 100.0 NP_055591 679 608564 99.6 100.0 XP_006719772 709 608564 95.4 100.0 NP_001317082 708 608564 95.4 100.0 XP_006719775 596 608564 87.4 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA0148 Download

>KIAA0148  2043 bp
ATGTCGAAACGGCTCCGGAGCAGCGAGGTGTGCGCTGACTGCAGCGGGCCGGATCCTTCC
TGGGCATCAGTAAATAGGGGAACGTTTTTATGTGATGAGTGCTGCAGTGTCCATCGGAGT
CTAGGGCGCCATATCTCCCAAGTGAGGCATCTGAAACACACACCGTGGCCTCCAACACTG
CTTCAGATGGTTGAGACCTTGTATAATAACGGTGCTAACTCTATATGGGAGCATTCTTTG
CTGGACCCTGCGTCTATTATGAGTGGAAGACGTAAAGCTAATCCACAGGATAAAGTACAT
CCCAATAAAGCGGAATTCATCAGAGCCAAGTATCAGATGTTAGCGTTCGTCCATCGCTTG
CCCTGCCGGGATGACGATAGTGTGACTGCCAAAGATCTTAGCAAGCAACTCCATTCGAGC
GTGAGAACAGGGAATCTTGAAACCTGTTTGAGACTGTTATCTTTAGGAGCACAAGCCAAC
TTCTTTCATCCTGAAAAAGGAAACACCCCACTCCATGTTGCCTCCAAAGCAGGGCAGATT
TTACAGGCTGAATTATTGGCAGTATATGGAGCAGACCCAGGCACACAGGATTCTAGTGGG
AAAACTCCCGTTGATTATGCAAGGCAAGGAGGGCACCATGAGCTGGCAGAGCGCCTCGTG
GAAATACAGTATGAGCTAACGGACAGACTAGCCTTCTATCTCTGTGGCAGGAAACCAGAT
CACAAAAATGGACAGCACTTTATAATACCTCAAATGGCAGACAGACGGCTCAGCCTGGAT
TTGTCTGAATTGGCAAAAGCTGCTAAGAAGAAACTTCAATCTCTAAGTAATCATTTGTTT
GAAGAACTTGCCATGGATGTGTACGATGAAGTTGACAGGCGAGAGACGGATGCAGTCTGG
CTTGCCACGCAAAACCACAGCGCCCTGGTAACCGAGACAACGGTCGTCCCCTTTCTTCCG
GTCAATCCTGAGTACTCATCAACACGAAATCAGGGCAGACAGAAGTTAGCTCGGTTCAAC
GCCCATGAGTTTGCCACGCTGGTCATTGACATTCTCAGTGACGCCAAGAGGAGACAGCAG
GGCAGTTCTCTCTCGGGTTCAAAAGACAATGTGGAGCTCATACTGAAAACCATCAATAAC
CAGCACAGCGTTGAGAGTCAAGACAACGATCAGCCCGACTATGACAGCGTGGCATCAGAC
GAAGACACAGATTTGGAAACCACTGCAAGCAAAACAAACCGGCAGAAGCTTCAAACACTC
CAGAGTGAAAATTCGAACCTCAGGAAACAGGCCACAACCAATGTATATCAGGTGCAAACT
GGTTCTGAGTACACAGACACTTCCAACCACTCTTCCTTAAAGAGACGTCCGTCTGCCCGG
GGCAGTAGGCCCATGTCCATGTACGAGACCGGATCAGGTCAGAAACCATATCTCCCAATG
GGAGAAGCGAGCCGCCCCGAAGAGAGCAGGATGAGACTCCAGCCCTTCCCCGCGCACGCA
TCCAGGCTGGAGAAGCAGAACAGCACACCTGAGAGTGACTACGACAACACTCCCAACGAC
ATGGAGCCAGATGGCATGGGGTCAAGCCGAAAGGGACGGCAAAGAAGTATGGTGTGGCCA
GGGGATGGCTTGGTACCAGACACAGCAGAACCCCATGTGGCCCCAAGCCCCACTCTCCCT
AGCACCGAAGATGTCATCAGGAAGACTGAACAGATCACCAAAAACATACAGGAGCTCTTA
AGAGCAGCCCAAGAAAATAAACATGACAGTTATATTCCCTGCTCAGAGAGGATACACGTA
GCTGTTACAGAAATGGCAGCATTATTCCCCAAAAAACCCAAGTCTGATATGGTGAGGACT
TCCCTTCGTTTACTGACGTCCAGTGCCTACCGACTGCAGTCAGAGTGCAAGAAGACCCTC
CCAGGGGACCCCGGCTCACCCACAGACGTTCAGCTGGTCACGCAGCAGGTCATCCAGTGT
GCGTACGACATCGCCAAGGCTGCCAAGCAGCTGGTTACCATCACCACCAAAGAGAACAAC
AAC

ORF Protein

Cloned ORF protein sequence for pFN21AA0148 Download

>KIAA0148  681 aa
MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTL
LQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRL
PCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQI
LQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPD
HKNGQHFIIPQMADRRLSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVW
LATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQ
GSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKLQTL
QSENSNLRKQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPM
GEASRPEESRMRLQPFPAHASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKGRQRSMVWP
GDGLVPDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHV
AVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPGSPTDVQLVTQQVIQC
AYDIAKAAKQLVTITTKENNN

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA0148 Download

>pFN21AA0148  6748 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCGAAACGGCTCCGGAGCAGCGAGGTGTGCGC
TGACTGCAGCGGGCCGGATCCTTCCTGGGCATCAGTAAATAGGGGAACGTTTTTATGTGA
TGAGTGCTGCAGTGTCCATCGGAGTCTAGGGCGCCATATCTCCCAAGTGAGGCATCTGAA
ACACACACCGTGGCCTCCAACACTGCTTCAGATGGTTGAGACCTTGTATAATAACGGTGC
TAACTCTATATGGGAGCATTCTTTGCTGGACCCTGCGTCTATTATGAGTGGAAGACGTAA
AGCTAATCCACAGGATAAAGTACATCCCAATAAAGCGGAATTCATCAGAGCCAAGTATCA
GATGTTAGCGTTCGTCCATCGCTTGCCCTGCCGGGATGACGATAGTGTGACTGCCAAAGA
TCTTAGCAAGCAACTCCATTCGAGCGTGAGAACAGGGAATCTTGAAACCTGTTTGAGACT
GTTATCTTTAGGAGCACAAGCCAACTTCTTTCATCCTGAAAAAGGAAACACCCCACTCCA
TGTTGCCTCCAAAGCAGGGCAGATTTTACAGGCTGAATTATTGGCAGTATATGGAGCAGA
CCCAGGCACACAGGATTCTAGTGGGAAAACTCCCGTTGATTATGCAAGGCAAGGAGGGCA
CCATGAGCTGGCAGAGCGCCTCGTGGAAATACAGTATGAGCTAACGGACAGACTAGCCTT
CTATCTCTGTGGCAGGAAACCAGATCACAAAAATGGACAGCACTTTATAATACCTCAAAT
GGCAGACAGACGGCTCAGCCTGGATTTGTCTGAATTGGCAAAAGCTGCTAAGAAGAAACT
TCAATCTCTAAGTAATCATTTGTTTGAAGAACTTGCCATGGATGTGTACGATGAAGTTGA
CAGGCGAGAGACGGATGCAGTCTGGCTTGCCACGCAAAACCACAGCGCCCTGGTAACCGA
GACAACGGTCGTCCCCTTTCTTCCGGTCAATCCTGAGTACTCATCAACACGAAATCAGGG
CAGACAGAAGTTAGCTCGGTTCAACGCCCATGAGTTTGCCACGCTGGTCATTGACATTCT
CAGTGACGCCAAGAGGAGACAGCAGGGCAGTTCTCTCTCGGGTTCAAAAGACAATGTGGA
GCTCATACTGAAAACCATCAATAACCAGCACAGCGTTGAGAGTCAAGACAACGATCAGCC
CGACTATGACAGCGTGGCATCAGACGAAGACACAGATTTGGAAACCACTGCAAGCAAAAC
AAACCGGCAGAAGCTTCAAACACTCCAGAGTGAAAATTCGAACCTCAGGAAACAGGCCAC
AACCAATGTATATCAGGTGCAAACTGGTTCTGAGTACACAGACACTTCCAACCACTCTTC
CTTAAAGAGACGTCCGTCTGCCCGGGGCAGTAGGCCCATGTCCATGTACGAGACCGGATC
AGGTCAGAAACCATATCTCCCAATGGGAGAAGCGAGCCGCCCCGAAGAGAGCAGGATGAG
ACTCCAGCCCTTCCCCGCGCACGCATCCAGGCTGGAGAAGCAGAACAGCACACCTGAGAG
TGACTACGACAACACTCCCAACGACATGGAGCCAGATGGCATGGGGTCAAGCCGAAAGGG
ACGGCAAAGAAGTATGGTGTGGCCAGGGGATGGCTTGGTACCAGACACAGCAGAACCCCA
TGTGGCCCCAAGCCCCACTCTCCCTAGCACCGAAGATGTCATCAGGAAGACTGAACAGAT
CACCAAAAACATACAGGAGCTCTTAAGAGCAGCCCAAGAAAATAAACATGACAGTTATAT
TCCCTGCTCAGAGAGGATACACGTAGCTGTTACAGAAATGGCAGCATTATTCCCCAAAAA
ACCCAAGTCTGATATGGTGAGGACTTCCCTTCGTTTACTGACGTCCAGTGCCTACCGACT
GCAGTCAGAGTGCAAGAAGACCCTCCCAGGGGACCCCGGCTCACCCACAGACGTTCAGCT
GGTCACGCAGCAGGTCATCCAGTGTGCGTACGACATCGCCAAGGCTGCCAAGCAGCTGGT
TACCATCACCACCAAAGAGAACAACAACGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT